Use of a hyperbolic grid generation scheme in simulating supersonic viscous flow about three-dimensional winged configuration

The present paper describes a numerical mesh generation technique to be used with an implicit finite difference method for simulating visous supersonic flow about low-aspect-ratio wing body configurations using a single grid strategy. The computational domain is segmented into multiple regions, with borders located in supersonic areas to avoid the otherwise costly interfacing procedure between adjacent segments. The numerical procedure is applied to calculate the turbulent flow around the shuttle orbiter and a canard projectile at supersonic free stream Mach number

Parámetros de espectros U.V. para investigar las influencias de intensificaciones de cultivos de soja con sorgo y maíz sobre las propiedades físicas de aceites de soja

Using U.V. spectra measurements as a tool to investigate the influences of intensifications or intercroping of crops on the physical properties of the constituents of the yielded grains or seeds. In this paper the authors used U.V. spectra measurement to deduce the effects of intensifications of Clark and Crowmord soybean varieties on some sorghum and maize varieties on the physical properties of the oils of the soybean seed varieties. Results of this work revealed that the highest U.V. spectra measurements at bands 270 nm, were 2.5 for the oils of Crowmord soybean seed variety intensificated on Giza 15 sorghum variety intensity 2:4 (2 rows soybean: 4 rows sorghum) and Giza 2, Hz 215 and Hz 310 maize varieties, intensity 2:2, 2:4, 2:4 and 2:2 respectively in the regions of spectra differences. The intensification of Clark and Crowmord soybean varieties on sorghum and maize is effective as the physical properties of their oils improved by increasing the cromophor and carotenoids compound levels.Se usaron medidas de espectros U.V. como una herramienta para investigar las influencias de intensificaciones o intercultivos sobre las propiedades físicas de los constituyentes de granos o semillas producidos. En este trabajo el autor usó medidas de espectros U.V. para deducir los efectos de intensificaciones de variedades de soja Clark y Crowmord con variedades de sorgo y maíz sobre las propiedades físicas de los aceites de soja. Los resultados de este trabajo revelaron que los valores de espectros U.V. más elevados a 270 nm, fueron 2'5 para los aceites de variedades de semilla de soja Crowmord intensificado con la variedad sorgo Giza 15 (2 filas soja: 4 filas sorgo) y las variedades de maíz Giza 2 (2:2 y 2:4), Hz 215 (2:4) y Hz 310 (2:2). La intensificación de variedades de soja Clark y Crowmord con sorgo y maíz es efectiva, ya que las propiedades físicas de sus aceites mejoraron por aumento de los niveles de compuestos cromóforos y carotenoides

Successful Use of Microporous Polytetrafluoroethylene Flexible Thin Sheets in NASA's OSIRIS-REx Mission

Microporous black polytetrafluoroethylene (PTFE) flexible thin sheets are successfully flown as solar diffusers on NASA's Origins, Spectral Interpretation, Resource Identification, and Security-Regolith Explorer (OSIRIS-REx) spacecraft. They serve as multilayer insulation (MLI) blanket outer covers for the arm of the Touch And Go Sample Acquisition Mechanism (TAGSAM), the sunshade of the OSIRIS-REx Camera Suite (OCAMS) PolyCam imager, and the motor riser of the OCAMS SamCam imager. Additionally, microporous white PTFE flexible thin sheets are successfully flown as a MLI blanket outer cover with a low ratio of absorptance to emittance for the Regolith X-ray Imaging Spectrometer (REXIS). For ground testing, microporous black and white PTFE flexible thin sheets were successfully used as optical targets of the Touch And Go Camera System (TAGCAMS) NavCam imagers in the flight system thermal vacuum test

Differentiating between models of Epothilone binding to microtubules using tubulin mutagenesis, cytotoxicity, and molecular modeling

This is the peer reviewed version of the following article: Entwistle, R. A., Rizk, R. S., Cheng, D. M., Lushington, G. H., Himes, R. H., & Gupta, M. L. (2012). Differentiating between models of Epothilone binding to microtubules using tubulin mutagenesis, cytotoxicity, and molecular modeling. ChemMedChem, 7(9), 1580–1586. http://doi.org/10.1002/cmdc.201200286, which has been published in final form at doi.org/10.1002/cmdc.201200286. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.Microtubule stabilizers are powerful anti-mitotic compounds and represent a proven cancer treatment strategy. Several classes of compounds in clinical use or trials, such as the taxanes and epothilones, bind to the same region of β-tubulin. Determining how these molecules interact with tubulin and stabilize microtubules is important both for understanding the mechanism of action and enhancing chemotherapeutic potential, e.g. reducing side effects, increasing solubility, and overcoming resistance. Structural studies using nonpolymerized tubulin or stabilized polymers have produced different models of epothilone binding. Here, we used directed mutagenesis of the binding site on Saccharomyces cerevisiae β-tubulin to analyze interactions between Epothilone B and its biologically relevant substrate, dynamic microtubules. Five engineered amino acid changes contributed to a 125-fold increase in Epothilone B cytotoxicity independent of inherent microtubule stability. The mutagenesis of endogenous β-tubulin was done in otherwise isogenic strains. This facilitated the correlation of amino acid substitutions with altered cytotoxicity using molecular mechanics simulations. The results, which are based on the interaction between Epothilone B and dynamic microtubules, most strongly support the binding mode determined by NMR spectroscopy-based studies. This work establishes a system for discriminating between potential binding modes and among various compounds and/or analogues using a sensitive biological activity-based readout

Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of beta-catenin in adrenocortical carcinoma

Adrenocortical cancer (ACC) is a very aggressive tumor, and genomics studies demonstrate that the most frequent alterations of driver genes in these cancers activate the Wnt/beta-catenin signaling pathway. However, the adrenal-specific targets of oncogenic beta-catenin-mediating tumorigenesis have not being established. A combined transcriptomic analysis from two series of human tumors and the human ACC cell line H295R harboring a spontaneous beta-catenin activating mutation was done to identify the Wnt/beta-catenin targets. Seven genes were consistently identified in the three studies. Among these genes, we found that AFF3 mediates the oncogenic effects of beta-catenin in ACC. The Wnt response element site located at nucleotide position - 1408 of the AFF3 transcriptional start sites (TSS) mediates the regulation by the Wnt/beta-catenin signaling pathway. AFF3 silencing decreases cell proliferation and increases apoptosis in the ACC cell line H295R. AFF3 is located in nuclear speckles, which play an important role in RNA splicing. AFF3 overexpression in adrenocortical cells interferes with the organization and/or biogenesis of these nuclear speckles and alters the distribution of CDK9 and cyclin T1 such that they accumulate at the sites of AFF3/speckles. We demonstrate that AFF3 is a new target of Wnt/beta-catenin pathway involved in ACC, acting on transcription and RNA splicing

Overcoming the Challenges Associated with Image-based Mapping of Small Bodies in Preparation for the OSIRIS-REx Mission to (101955) Bennu

The OSIRIS-REx Asteroid Sample Return Mission is the third mission in NASA's New Frontiers Program and is the first U.S. mission to return samples from an asteroid to Earth. The most important decision ahead of the OSIRIS-REx team is the selection of a prime sample-site on the surface of asteroid (101955) Bennu. Mission success hinges on identifying a site that is safe and has regolith that can readily be ingested by the spacecraft's sampling mechanism. To inform this mission-critical decision, the surface of Bennu is mapped using the OSIRIS-REx Camera Suite and the images are used to develop several foundational data products. Acquiring the necessary inputs to these data products requires observational strategies that are defined specifically to overcome the challenges associated with mapping a small irregular body. We present these strategies in the context of assessing candidate sample-sites at Bennu according to a framework of decisions regarding the relative safety, sampleability, and scientific value across the asteroid's surface. To create data products that aid these assessments, we describe the best practices developed by the OSIRIS-REx team for image-based mapping of irregular small bodies. We emphasize the importance of using 3D shape models and the ability to work in body-fixed rectangular coordinates when dealing with planetary surfaces that cannot be uniquely addressed by body-fixed latitude and longitude.Comment: 31 pages, 10 figures, 2 table

Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of beta-catenin in adrenocortical carcinoma

Adrenocortical cancer (ACC) is a very aggressive tumor, and genomics studies demonstrate that the most frequent alterations of driver genes in these cancers activate the Wnt/beta-catenin signaling pathway. However, the adrenal-specific targets of oncogenic beta-catenin-mediating tumorigenesis have not being established. A combined transcriptomic analysis from two series of human tumors and the human ACC cell line H295R harboring a spontaneous beta-catenin activating mutation was done to identify the Wnt/beta-catenin targets. Seven genes were consistently identified in the three studies. Among these genes, we found that AFF3 mediates the oncogenic effects of beta-catenin in ACC. The Wnt response element site located at nucleotide position - 1408 of the AFF3 transcriptional start sites (TSS) mediates the regulation by the Wnt/beta-catenin signaling pathway. AFF3 silencing decreases cell proliferation and increases apoptosis in the ACC cell line H295R. AFF3 is located in nuclear speckles, which play an important role in RNA splicing. AFF3 overexpression in adrenocortical cells interferes with the organization and/or biogenesis of these nuclear speckles and alters the distribution of CDK9 and cyclin T1 such that they accumulate at the sites of AFF3/speckles. We demonstrate that AFF3 is a new target of Wnt/beta-catenin pathway involved in ACC, acting on transcription and RNA splicing

Reachability in Biochemical Dynamical Systems by Quantitative Discrete Approximation (extended abstract)

In this paper, a novel computational technique for finite discrete approximation of continuous dynamical systems suitable for a significant class of biochemical dynamical systems is introduced. The method is parameterized in order to affect the imposed level of approximation provided that with increasing parameter value the approximation converges to the original continuous system. By employing this approximation technique, we present algorithms solving the reachability problem for biochemical dynamical systems. The presented method and algorithms are evaluated on several exemplary biological models and on a real case study.Comment: In Proceedings CompMod 2011, arXiv:1109.104