66 research outputs found

    Description of Maribacter forsetii sp nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter

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    Three rod-shaped, Gram-negative, chemo-organotrophic, heterotrophic, strictly aerobic, gliding bacterial strains, KT02ds18-4, KT02ds18-5 and KT02ds18-6T, were isolated from North Sea surface waters near the island of Helgoland, Germany. Their taxonomic position was investigated by a polyphasic approach. The three strains were light yellow, oxidase- and catalase-positive, and grew optimally at 25 degrees C, at pH 7.5, and in the presence of 2.5 % (w/v) NaCl. The Chargaff's coefficient was 34.2-34.4 mol%. The three strains shared >90 % DNA-DNA relatedness and an identical 16S rRNA gene sequence. Comparative 16S rRNA gene sequence analysis allocated the three strains to the genus Maribacter in the family Flavobacteriaceae, with similarities of 97.0-97.4 % to five of the recognized Maribacter species. Their low level of DNA-DNA relatedness (<20 %) with these species and differentiating phenotypic characteristics demonstrated that they constitute a new Maribacter species for which the name Maribacter forsetii sp. nov. is proposed. Strain KT02ds18-6T (=CIP 109504T=DSM 18668T) is the type strain. An emended description of the genus Maribacter is also proposed

    Genetic Epidemiology of Glioblastoma Multiforme: Confirmatory and New Findings from Analyses of Human Leukocyte Antigen Alleles and Motifs

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    Human leukocyte antigen (HLA) class I genes mediate cytotoxic T-lymphocyte responses and natural killer cell function. In a previous study, several HLA-B and HLA-C alleles and haplotypes were positively or negatively associated with the occurrence and prognosis of glioblastoma multiforme (GBM).As an extension of the Upper Midwest Health Study, we have performed HLA genotyping for 149 GBM patients and 149 healthy control subjects from a non-metropolitan population consisting almost exclusively of European Americans. Conditional logistic regression models did not reproduce the association of HLA-B*07 or the B*07-Cw*07 haplotype with GBM. Nonetheless, HLA-A*32, which has previously been shown to predispose GBM patients to a favorable prognosis, was negatively associated with occurrence of GBM (odds ratio=0.41, p=0.04 by univariate analysis). Other alleles (A*29, A*30, A*31 and A*33) within the A19 serology group to which A*32 belongs showed inconsistent trends. Sequencing-based HLA-A genotyping established that A*3201 was the single A*32 allele underlying the observed association. Additional evaluation of HLA-A promoter and exon 1 sequences did not detect any unexpected single nucleotide polymorphisms that could suggest differential allelic expression. Further analyses restricted to female GBM cases and controls revealed a second association with a specific HLA-B sequence motif corresponding to Bw4-80Ile (odds ratio=2.71, p=0.02).HLA-A allelic product encoded by A*3201 is likely to be functionally important to GBM. The novel, sex-specific association will require further confirmation in other representative study populations

    Data for: Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage.

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    Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage.Stéphane L’Haridona*, Léna Gouhiera, Emily St. Johnb and Anna-Louise ReysenbachbUniv Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280, Plouzané, Francea; Portland State University, Department of Biology and Center for Life in Extreme Environments, P.O. Box 751, Portland, OR 97207 USAb

    Data for: Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage.

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    Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage.Stéphane L’Haridona*, Léna Gouhiera, Emily St. Johnb and Anna-Louise ReysenbachbUniv Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280, Plouzané, Francea; Portland State University, Department of Biology and Center for Life in Extreme Environments, P.O. Box 751, Portland, OR 97207 USAb.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

    Data for: Parametric study of uniform and pitting corrosion of aluminium alloy 6061-T6 and characterisation of the hydroxide layers

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    Thicknesses of aluminium hydroxide after corrosion tests at differents temperatures and pH and chimical composition of the solutions.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

    Study of the influence of the initial pH on the aqueous corrosion of an Al-Mg-Si alloy at 70°C

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    International audienceAs many components of research nuclear reactors are composed of aluminium alloys, the study of the corrosion of aluminium alloys is important for their safe and efficient operation. In particular, at 70°C, pH can be optimized. In order to study the impact of initial pH of aqueous solutions on corrosion of an Al-Mg-Si alloy at 70°C, three corrosion experiments at different pH are performed on AA-6061-T6 alloy, in autoclaves for 33 days. Corrosion solutions with initial pH of 5, 6 and 7 are obtained by dilution of nitric acid in pure water. Various characterization techniques are used to study hydroxide phase, composition and structure. X-ray diffraction on corrosion product indicates bayerite (α-Al(OH3)) is the main crystalline phase formed during the corrosion experiments. Generalized corrosion thickness measured with Scanning Electron Microscopy are the same for all three pH. However, mass gain is most important for samples corroded at pH 6. This difference in mass gain is attributed to pitting corrosion caused by intermetallic Fe-Si-Al in the aluminum matrix. Detailed study of these precipitates by EDS has been performed in order to further understand corrosion mechanisms. From this study, suggestions on optimal pH for corrosion resistance can be proposed
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