The Role of Endophytic Fungi in \u3ci\u3eBrachiaria\u3c/i\u3e, a Tropical Forage Grass

In temperate zones, endophytic fungi are widely used as biological protection agents for forage and turf grasses. They form nonpathogenic and intercellular associations with grasses and sedges, completing their entire life cycle within the plants’ aerial parts. Our surveys and studies confirmed that various endophytic fungi, including Acremonium spp., also inhabit native savanna grasses and introduced tropical forage grasses. We are now determining the potentially symbiotic relationships between these fungi and C4 tropical forages, specifically between the endophyte A. implicatum and Brachiaria grasses. We treated half of a group of genetically identical clones of Brachiaria with fungicide to generate endophyte-free plants. So far, we have found that, under severe water stress, endophyte-infected plants of B. arrecta CIAT 16845 maintained better leaf expansion and produced significantly more leaf biomass than did clean plants. We also found that the endophyte protects B. brizantha from pathogenic fungi such as Drechslera sp. (causal agent of leaf spot), the grass showing fewer and smaller lesions than did endophyte-free plants. The endophyte also inhibits the growth of Rhizoctonia solani (causal agent of foliar blight in Brachiaria) and Pyricularia oryzae (causal agent of rice blast). It may even protect Brachiaria from pests such as the aphid Rhopalosiphum maidis. However, several years of research has shown that, in infected temperate grasses, endophytes reduce livestock productivity. Whether this is true for tropical forage grasses such as Brachiaria is not yet known, although what little evidence exists suggests that endophytes may cause various health disorders in livestock

Detecting bacterial endophytes in tropical grasses of the Brachiaria genus and determining their role in improving plant growth

Plant-growth-promoting (PGP) bacteria include a diverse group of soil bacteria thought to stimulate plant growth by various mechanisms. Brachiaria forage grasses, of African origin, are perennials that often grow under low-input conditions and are likely to harbour unique populations of PGP bacteria. Three bacterial strains that tested positive for nitrogenase reductase gene sequences (nifH) were isolated from Brachiaria hybrid CIAT 36062 and introduced into Brachiaria hybrid cv. Mulato, which also had indigenous endophytic bacteria testing positive for nifH gene sequences. Under conditions of nutrient deficiency, inoculated Mulato plants had significantly higher biomass production, chlorophyll and total nitrogen contents in leaves than do control plants and were darker green. Strains ofendophytic bacteria were then artificially introduced into Brachiaria  brizantha CIAT 6294, which does not have indigenous endophytic bacteria. Results were consistent with those obtained with artificially inoculated Mulato plants, suggesting that these endophytic bacteria do benefit plant growth. DNA sequence analysis demonstrated that the nifH gene sequences were highly similar to those from Klebsiella pneumoniae and other N2-fixing organisms and that the nif genes had consensus  sequences identical to those of other N2-fixing bacteria.Key words: Bacterial endophytes, green fluorescent protein, nitrogenase reductase, plant growth-promoting bacteria, Brachiaria

Genetic Diversity in the Anthracnose Pathogen Infecting \u3ci\u3eStylosanthes\u3c/i\u3e in Brazil, India and China

This work aimed to determine the genetic diversity of Colletotrichum gloeosporioides infecting Stylosanthes spp. in Brazil, China and India. A total of 132 isolate originating from S. seabrana, S. macrocephala, S. capitata, S. scabra, and S. guianensis were used. Four major genetic groups were identified from an analysis of genetic diversity using selection-neutral DNA markers. Group 1 contained 20 isolates and this may represent a genotype that migrated from the center of diversity in Brazil and Colombia to Australia, Thailand and India. Group 2 consisted of 66 Brazilian isolates and group 3 had 19 isolates from Australia, Burundi, Brazil, China, Colombia, Ivory Coast and Peru. The 27 isolates in group 4 were very diverse with \u3e50% dissimilarity between some isolates. Genetic diversity in Brazil and China was more extensive than in the Indian pathogen population

Global overview of locusts as food, feed and other uses

This review assesses the potential of harnessing locust swarms for beneficial uses as a more sustainable management strategy than using pesticides. As well, it highlights the global distribution of locust species; their nutritional value; historical practices of their use as food, feed and other applications; harvesting technologies; and regulatory framework. Locusts have traditionally been consumed by humans or fed to animals for millennia. The nutritional composition is comparable or superior to that of conventional meat. They are potential raw materials for chitin, oil and nutraceutical products. Safety concerns in the beneficial use of locusts are insecticides, allergens and microbial contaminants.Australian Centre for International Agricultural ResearchNorwegian Agency for Development CooperationBioinnovate Africa Programme through SIDARockefeller FoundationUnited Kingdom’s Foreign, Commonwealth & Development OfficeSwedish International Development Cooperation AgencySwiss Agency for Development and CooperationFederal Democratic Republic of EthiopiaGovernment of the Republic of Keny

A maize landrace that emits defense volatiles in response to herbivore eggs possesses a strongly inducible terpene synthase gene.

Maize (Zea mays) emits volatile terpenes in response to insect feeding and egg deposition to defend itself against harmful pests. However, maize cultivars differ strongly in their ability to produce the defense signal. To further understand the agroecological role and underlying genetic mechanisms for variation in terpene emission among maize cultivars, we studied the production of an important signaling component (E)-caryophyllene in a South American maize landrace Braz1006 possessing stemborer Chilo partellus egg inducible defense trait, in comparison with the European maize line Delprim and North American inbred line B73. The (E)-caryophyllene production level and transcript abundance of TPS23, terpene synthase responsible for (E)-caryophyllene formation, were compared between Braz1006, Delprim, and B73 after mimicked herbivory. Braz1006-TPS23 was heterologously expressed in E. coli, and amino acid sequences were determined. Furthermore, electrophysiological and behavioral responses of a key parasitic wasp Cotesia sesamiae to C. partellus egg-induced Braz1006 volatiles were determined using coupled gas chromatography electroantennography and olfactometer bioassay studies. After elicitor treatment, Braz1006 released eightfold higher (E)-caryophyllene than Delprim, whereas no (E)-caryophyllene was detected in B73. The superior (E)-caryophyllene production by Braz1006 was positively correlated with high transcript levels of TPS23 in the landrace compared to Delprim. TPS23 alleles from Braz1006 showed dissimilarities at different sequence positions with Delprim and B73 and encodes an active enzyme. Cotesia sesamiae was attracted to egg-induced volatiles from Braz1006 and synthetic (E)-caryophyllene. The variation in (E)-caryophyllene emission between Braz1006 and Delprim is positively correlated with induced levels of TPS23 transcripts. The enhanced TPS23 activity and corresponding (E)-caryophyllene production by the maize landrace could be attributed to the differences in amino acid sequence with the other maize lines. This study suggested that the same analogous genes could have contrasting expression patterns in different maize genetic backgrounds. The current findings provide valuable insight not only into genetic mechanisms underlying variation in defense signal production but also the prospect of introgressing the novel defense traits into elite maize varieties for effective and ecologically sound protection of crops against damaging insect pests

Exploring black soldier fly frass as novel fertilizer for improved growth, yield, and nitrogen use efficiency of maize under field conditions

Open Access Journal; Published online: 23 Sept 2020Black soldier fly frass fertilizer (BSFFF) is increasingly gaining momentum worldwide as organic fertilizer. However, research on its performance on crop production remains largely unknown. Here, we evaluate the comparative performance of BSFFF and commercial organic fertilizer (SAFI) on maize (H513) production. Both fertilizers were applied at the rates of 0, 2.5, 5, and 7.5 t ha-1, and 0, 30, 60, and 100 kg nitrogen (N) ha-1. Mineral fertilizer (urea) was also applied at 0, 30, 60 and 100 kg N ha-1 to establish the N fertilizer equivalence (NFE) of the organic fertilizers. Maize grown in plots treated with BSFFF had the tallest plants and highest chlorophyll concentrations. Plots treated with 7.5 t ha-1 of BSFFF had 14% higher grain yields than plots treated with a similar rate of SAFI. There was a 27% and 7% increase in grain yields in plots treated with 100 kg N ha-1 of BSFFF compared to those treated with equivalent rates of SAFI and urea fertilizers, respectively. Application of BSFFF at 7.5 t ha-1 significantly increased N uptake by up to 23% compared to the equivalent rate of SAFI. Likewise, application of BSFFF at 100 kg N ha-1 increased maize N uptake by 76% and 29% compared to SAFI and urea, respectively. Maize treated with BSFFF at 2.5 t ha-1 and 30 kg N ha-1 had higher nitrogen recovery efficiencies compared to equivalent rates of SAFI. The agronomic N use efficiency (AEN) of maize treated with 2.5 t ha-1 of BSFFF was 2.4 times higher than the value achieved using an equivalent rate of SAFI. Also, the AEN of maize grown using 30 kg N ha-1 was 27% and 116% higher than the values obtained using equivalent rates of SAFI and urea fertilizers, respectively. The NFE of BSFFF (108%) was 2.5 times higher than that of SAFI. Application rates of 2.5 t ha-1 and 30 kg N ha-1 of BSFFF were found to be effective in improving maize yield, while double rates of SAFI were required. Our findings demonstrate that BSFFF is a promising and sustainable alternative to commercial fertilizers for increased maize production

Harnessing data science to improve integrated management of invasive pest species across Africa: an application to Fall armyworm (Spodoptera frugiperda) (J.E. Smith) (Lepidoptera: Noctuidae)

Open Access Journal; Published online: 11 Feb 2022After five years of its first report on the African continent, Fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith) is considered a major threat to maize, sorghum, and millet production in sub-Saharan Africa. Despite the rigorous work already conducted to reduce FAW prevalence, the dynamics and invasion mechanisms of FAW in Africa are still poorly understood. This study applied interdisciplinary tools, analytics, and algorithms on a FAW dataset with a spatial lens to provide insights and project the intensity of FAW infestation across Africa. The data collected between January 2018 and December 2020 in selected locations were matched with the monthly average data of the climatic and environmental variables. The multilevel analytics aimed to identify the key factors that influence the dynamics of spatial and temporal pest density and occurrence at a 2 km x 2 km grid resolution. The seasonal variations of the identified factors and dynamics were used to calibrate rule-based analytics employed to simulate the monthly densities and occurrence of the FAW for the years 2018, 2019, and 2020. Three FAW density level classes were inferred, i.e., low (0–10 FAW moth per trap), moderate (11–30 FAW moth per trap), and high (>30 FAW moth per trap). Results show that monthly density projections were sensitive to the type of FAW host vegetation and the seasonal variability of climatic factors. Moreover, the diversity in the climate patterns and cropping systems across the African sub-regions are considered the main drivers of FAW abundance and variation. An optimum overall accuracy of 53% was obtained across the three years and at a continental scale, however, a gradual increase in prediction accuracy was observed among the years, with 2020 predictions providing accuracies greater than 70%. Apart from the low amount of data in 2018 and 2019, the average level of accuracy obtained could also be explained by the non-inclusion of data related to certain key factors such as the influence of natural enemies (predators, parasitoids, and pathogens) into the analysis. Further detailed data on the occurrence and efficiency of FAW natural enemies in the region may help to complete the tri-trophic interactions between the host plants, pests, and beneficial organisms. Nevertheless, the tool developed in this study provides a framework for field monitoring of FAW in Africa that may be a basis for a future decision support system (DSS)