Calcibiocavitological investigations

Calcibiocavitation is a major poroblem in the marine environment and a detailed study on these aspects has been undertaken and the salient findings are presented here. Gregarious molluscs such as the sacred chank Xancus pvrum, mussels (both green and brown), rock oysters (Crassostrea spp.), pearl oysters, Thais rudolphi (Lam.) and corals which inhabit the southwest coast of Kerala and the Gulf of Mannar have been investigated. The wide fluctuations noted in the abundance and population structure of the various pests in the molluscan beds during the short period of two years clearly indicated that they were in severe competetion for suitable substrata and the shells of gregarious mollusks which inhabit this area provide ample opportunities for the pests to flourish. The incidence of boring sponges is found to be rather high among raft-cultured pearl oysters both at Tuticorin and Vizhinjam. It is difficult to control the infection of boring animals in the natural beds, but the low rate of incidence recorded year after year under suggests that the nature controls this to lower level.Another important observation was the wide distribution of the boring sponge C.vastifica in the Ashtamudi Lake, Quilonwhich may form a major threat to our future rock oyster farms along the estuaries

The edible oyster culture

In 1970s the CMFR Institute initiated oyster culture through rack and tray method. Large scale spat collection, preparation of spat collectors, season and seed availability were studied. In 1980, a hatchery was set up with annual production capacity of one million edible oyster seed and the techniques involved in hatchery system were standardized. The seed were supplied to other centres of this Institute and Gujarat Fisheries Department. The production rates for rack and tray, string and stake method were estimated as 120 t and 22 t respectively. As part of extension, one lab to land programme, 8 training programmes, one workshop and a summer insitute were conducted. Results of experimental oyster culture work carried out at Mandapam, Madras, Bheemunipatnam, Kakinada, Mulki, Dharmadam, Ashtamudi and Cochin backwaters are given. Rearing 600 strings in 0.04 ha at Ashtamudi and harvesting 2.5t oysters pointed out 44.8% return with estimated production of 80- 105t/ha. Further research priorities on oyster culture aspects are indicated

Experience paper on the operation of pilot project on oyster culture at Tuticorin

The technology of oyster culture was developed in India at the Central Marine Fisheries Research Institute. An experimental farm was established at Tuticorin bay in an area of 0.25 hectares. Different growout methods have been tried and it has been assessed that the shell string method could be practised with relatively low cost inputs with a production rate comparable to highly efficient systems such as rack and tray method or raft culture

Invasion of Cliona margaritifera Dendy and C.lobata Hancock on the molluscan beds along the Indian coast

In the recent past the invasion of two new sponge pests Cliona margaritifera Dendy and C. lobata Hancock on the molluscan beds of the southwest coast of India is reported. These two new pests made their first appearance on cultured pearl oysters on raft at Vizhinjam in 1980 and thence started spreading to the economically important molluscan beds in and around Vizhinjam. The spreading of these pests along the southwest coast of India was rather fast and from this coast C. margaritifera could migrate to the raft-cultured pear! oysters at Tuticorin and C. lobata to the chank beds off Tbiruchendur (southeast coast) within two years i.e. by 1982

Experimental study of the settlement and collection Of pearl oyster spat from Tuticorin area

For the first time settlement and growth of pearl oyst«rs have be-en observed on granite stones forming the embankments of the New Tuticorin Port. Large numbers of pearl oyster spat have also been collected by employing diflfeirent types of spat collectors and the rate of growth of the oysters in the farm has been studied

Improving the interpretation of afternoon cortisol levels and SSTs to prevent misdiagnosis of Adrenal Insufficiency

Introduction Adrenal Insufficiency (AI), especially iatrogenic-AI, is a treatable cause of mortality. The difficulty in obtaining 9am cortisol levels means samples are taken at suboptimal times, including a substantial proportion in the afternoon. Low afternoon cortisol levels often provoke short Synacthen Tests (SSTs). It is important that this does not lead to patients misdiagnosed with AI, exposing them to the excess mortality and morbidity of inappropriate steroid replacement therapy. Methods This retrospective study collected 60,178 cortisol results. Medical records, including subsequent SSTs of initial cortisol results measured after midday were reviewed. Results ROC analysis (AUC- 0.89) on 6531 suitable cortisol values showed that a limit of 95% on the Abbott analyser platform. Conclusion An afternoon cortisol >234nmol/L excludes AI on Abbott analyser platforms. In patients who have an afternoon cortisol <234nmol/L, including both a 30-minute and a 60-minute SST cortisol values prevents unnecessary glucocorticoid replacement therapy in 22.3% of individuals in this study. The Abbott analyser SST cortisol cut-offs used to define AI should be 366.5nmol/L and 418.5nmol/L at 30- and 60-minutes respectively. All patients remained well subsequently with at least one year longitudinal follow up

Maternal Anti-Dengue IgG Fucosylation Predicts Susceptibility to Dengue Disease in Infants

Infant mortality from dengue disease is a devastating global health burden that could be minimized with the ability to identify susceptibility for severe disease prior to infection. Although most primary infant dengue infections are asymptomatic, maternally derived anti-dengue immunoglobulin G (IgGs) present during infection can trigger progression to severe disease through antibody-dependent enhancement mechanisms. Importantly, specific characteristics of maternal IgGs that herald progression to severe infant dengue are unknown. Here, we define \u3e /=10% afucosylation of maternal anti-dengue IgGs as a risk factor for susceptibility of infants to symptomatic dengue infections. Mechanistic experiments show that afucosylation of anti-dengue IgGs promotes FcgammaRIIIa signaling during infection, in turn enhancing dengue virus replication in FcgammaRIIIa(+) monocytes. These studies identify a post-translational modification of anti-dengue IgGs that correlates with risk for symptomatic infant dengue infections and define a mechanism by which afucosylated antibodies and FcgammaRIIIa enhance dengue infections

Pharmacological levels of withaferin A (Withania somnifera) trigger clinically relevant anticancer effects specific to triple negative breast cancer cells

Withaferin A (WA) isolated from Withania somnifera (Ashwagandha) has recently become an attractive phytochemical under investigation in various preclinical studies for treatment of different cancer types. In the present study, a comparative pathway-based transcriptome analysis was applied in epithelial-like MCF-7 and triple negative mesenchymal MDA-MB-231 breast cancer cells exposed to different concentrations of WA which can be detected systemically in in vivo experiments. Whereas WA treatment demonstrated attenuation of multiple cancer hallmarks, the withanolide analogue Withanone (WN) did not exert any of the described effects at comparable concentrations. Pathway enrichment analysis revealed that WA targets specific cancer processes related to cell death, cell cycle and proliferation, which could be functionally validated by flow cytometry and real-time cell proliferation assays. WA also strongly decreased MDA-MB-231 invasion as determined by single-cell collagen invasion assay. This was further supported by decreased gene expression of extracellular matrix-degrading proteases (uPA, PLAT, ADAM8), cell adhesion molecules (integrins, laminins), pro-inflammatory mediators of the metastasis-promoting tumor microenvironment (TNFSF12, IL6, ANGPTL2, CSF1R) and concomitant increased expression of the validated breast cancer metastasis suppressor gene (BRMS1). In line with the transcriptional changes, nanomolar concentrations of WA significantly decreased protein levels and corresponding activity of uPA in MDA-MB-231 cell supernatant, further supporting its anti-metastatic properties. Finally, hierarchical clustering analysis of 84 chromatin writer-reader-eraser enzymes revealed that WA treatment of invasive mesenchymal MDA-MB-231 cells reprogrammed their transcription levels more similarly towards the pattern observed in non-invasive MCF-7 cells. In conclusion, taking into account that sub-cytotoxic concentrations of WA target multiple metastatic effectors in therapy-resistant triple negative breast cancer, WA-based therapeutic strategies targeting the uPA pathway hold promise for further (pre)clinical development to defeat aggressive metastatic breast cancer