580 research outputs found
The SOD2 C47T polymorphism influences NAFLD fibrosis severity: evidence from case-control and intra-familial allele association studies.
AIMS:
Non-alcoholic fatty liver disease (NAFLD) is a complex disease trait where genetic variations and environment interact to determine disease progression. The association of PNPLA3 with advanced disease has been consistently demonstrated but many other modifier genes remain unidentified. In NAFLD, increased fatty acid oxidation produces high levels of reactive oxygen species. Manganese-dependent superoxide dismutase (MnSOD), encoded by the SOD2 gene, plays an important role in protecting cells from oxidative stress. A common non-synonymous polymorphism in SOD2 (C47T; rs4880) is associated with decreased MnSOD mitochondrial targeting and activity making it a good candidate modifier of NAFLD severity.
METHODS:
The relevance of the SOD2 C47T polymorphism to fibrotic NAFLD was assessed by two complementary approaches: we sought preferential transmission of alleles from parents to affected children in 71 family trios and adopted a case-control approach to compare genotype frequencies in a cohort of 502 European NAFLD patients.
RESULTS:
In the family study, 55 families were informative. The T allele was transmitted on 47/76 (62%) possible occasions whereas the C allele was transmitted on only 29/76 (38%) occasions, p=0.038. In the case control study, the presence of advanced fibrosis (stage>1) increased with the number of T alleles, p=0.008 for trend. Multivariate analysis showed susceptibility to advanced fibrotic disease was determined by SOD2 genotype (OR 1.56 (95% CI 1.09-2.25), p=0.014), PNPLA3 genotype (p=0.041), type 2 diabetes mellitus (p=0.009) and histological severity of NASH (p=2.0×10(-16)).
CONCLUSIONS:
Carriage of the SOD2 C47T polymorphism is associated with more advanced fibrosis in NASH
Reconstruction of a function from its spherical (circular) means with the centers lying on the surface of certain polygons and polyhedra
We present explicit filtration/backprojection-type formulae for the inversion
of the spherical (circular) mean transform with the centers lying on the
boundary of some polyhedra (or polygons, in 2D). The formulae are derived using
the double layer potentials for the wave equation, for the domains with certain
symmetries. The formulae are valid for a rectangle and certain triangles in 2D,
and for a cuboid, certain right prisms and a certain pyramid in 3D. All the
present inversion formulae yield exact reconstruction within the domain
surrounded by the acquisition surface even in the presence of exterior sources.Comment: 9 figure
Uniqueness of reconstruction and an inversion procedure for thermoacoustic and photoacoustic tomography
The paper contains a simple approach to reconstruction in Thermoacoustic and
Photoacoustic Tomography. The technique works for any geometry of point
detectors placement and for variable sound speed satisfying a non-trapping
condition. A uniqueness of reconstruction result is also obtained
Thermoacoustic tomography with variable sound speed
We study the mathematical model of thermoacoustic tomography in media with a
variable speed for a fixed time interval, greater than the diameter of the
domain. In case of measurements on the whole boundary, we give an explicit
solution in terms of a Neumann series expansion. We give necessary and
sufficient conditions for uniqueness and stability when the measurements are
taken on a part of the boundary
On the injectivity of the circular Radon transform arising in thermoacoustic tomography
The circular Radon transform integrates a function over the set of all
spheres with a given set of centers. The problem of injectivity of this
transform (as well as inversion formulas, range descriptions, etc.) arises in
many fields from approximation theory to integral geometry, to inverse problems
for PDEs, and recently to newly developing types of tomography. The article
discusses known and provides new results that one can obtain by methods that
essentially involve only the finite speed of propagation and domain dependence
for the wave equation.Comment: To appear in Inverse Problem
Genomic catastrophes frequently arise in esophageal adenocarcinoma and drive tumorigenesis
Oesophageal adenocarcinoma (EAC) incidence is rapidly increasing in Western countries. A better understanding of EAC underpins efforts to improve early detection and treatment outcomes. While large EAC exome sequencing efforts to date have found recurrent loss-offunction mutations, oncogenic driving events have been underrepresented. Here we use a combination of whole-genome sequencing (WGS) and single-nucleotide polymorphism-array profiling to show that genomic catastrophes are frequent in EAC, with almost a third (32%, n¼40/123) undergoing chromothriptic events. WGS of 22 EAC cases show that catastrophes may lead to oncogene amplification through chromothripsis-derived double-minute chromosome formation (MYC and MDM2) or breakage-fusion-bridge (KRAS, MDM2 and RFC3). Telomere shortening is more prominent in EACs bearing localized complex rearrangements. Mutational signature analysis also confirms that extreme genomic instability in EAC can be driven by somatic BRCA2 mutations. These findings suggest that genomic catastrophes have a significant role in the malignant transformation of EAC
Thermoacoustic tomography arising in brain imaging
We study the mathematical model of thermoacoustic and photoacoustic
tomography when the sound speed has a jump across a smooth surface. This models
the change of the sound speed in the skull when trying to image the human
brain. We derive an explicit inversion formula in the form of a convergent
Neumann series under the assumptions that all singularities from the support of
the source reach the boundary
Developing an intervention to facilitate family communication about inherited genetic conditions, and training genetic counsellors in its delivery.
Many families experience difficulty in talking about an inherited genetic condition that affects one or more of them. There have now been a number of studies identifying the issues in detail, however few have developed interventions to assist families. The SPRinG collaborative have used the UK Medical Research Council's guidance on Developing and Evaluating Complex Interventions, to work with families and genetic counsellors (GCs) to co-design a psycho-educational intervention to facilitate family communication and promote better coping and adaptation to living with an inherited genetic condition for parents and their children (<18 years). The intervention is modelled on multi-family discussion groups (MFDGs) used in psychiatric settings. The MFDG was developed and tested over three phases. First focus groups with parents, young people, children and health professionals discussed whether MFDG was acceptable and proposed a suitable design. Using evidence and focus group data, the intervention and a training manual were developed and three GCs were trained in its delivery. Finally, a prototype MFDG was led by a family therapist and co-facilitated by the three GCs. Data analysis showed that families attending the focus groups and intervention thought MFDG highly beneficial, and the pilot sessions had a significant impact on their family' functioning. We also demonstrated that it is possible to train GCs to deliver the MFDG intervention. Further studies are now required to test the feasibility of undertaking a definitive randomised controlled trial to evaluate its effectiveness in improving family outcomes before implementing into genetic counselling practice.The National Institute of Health Research funded the study but any views expressed do not necessarily reflect those of the Authority. Funded by NIHR reference number: RP-DG-1211-10015
Lymphangiogenesis in myocardial remodelling after infarction
Ishikawa Y, Akishima-Fukasawa Y, Ito K, Akasaka Y, Tanaka M, Shimokawa R, Kimura-Matsumoto M, Morita H, Sato S, Kamata I & Ishii T (2007) Histopathology51, 345–35
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An empirical algorithm for light absorption by ocean water based on color
Empirical algorithms for the total absorption coefficient and absorption
coefficient by pigments for surface waters at 440 nm were developed by applying a quadratic formula that combines two spectral ratios of remote-sensing reflectance. For
total absorption coefficients ranging from 0.02 to 2.0 m⁻¹, a goodness of fit was achieved
between the measured and modeled data with a root-mean-squared difference between the
measured and modeled values for log10 scale(RMSDₗₒ₁₀) of 0.062 (15.3% for linear
scale, number of samples N = 63), while RMSDₗₒ₁₀ is 0.111 ( 29.1% for linear scale,
N = 126) for pigment absorption (ranging from 0.01 to 1.0 m⁻¹). As alternatives to
pigment concentration algorithms, the absorption algorithms developed can be applied to
the coastal zone color scanner and sea-viewing wide-field-of-view sensor data to derive
inherent optical properties of the ocean. For the same data sets, we also directly related
the chlorophyll a concentrations to the spectral ratios and obtained an RMSDₗₒ₁₀ value
of 0.218 (65.2% for linear scale, N = 120) for concentrations ranging from 0.06 to 50.0
mg m⁻³. These results indicate that it is more accurate to estimate the absorption
coefficients than the pigment concentrations from remotely sensed data. This is likely due
to the fact that for the broad range of waters studied the pigment-specific absorption
coefficient at 440nm ranged from 0.02 to 0.2 m² (mg chl)⁻¹. As an indirect test of the
algorithms developed, the chlorophyll a concentration algorithm is applied to an
independent global dataset and an RMSDₗₒ₁₀ of 0.191( 55.2% for linear scale, N = 919)
is obtained. There is no independent global absorption data set available as yet to test the
absorption algorithms
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