Ultrastructure of intermediate stages in polarity reversal of thyroid epithelium in follicles in suspension culture.

Separated thyroid follicles can be maintained in suspension culture in Coon's modified F-12 medium in 0.5\% calf serum. If the serum concentration is raised to 5\%, the follicles undergo inversion in 3-5 d. During the process of inversion, epithelial cells can be observed in intermediate stages of polarity reversal. The earliest ultrastructural changes recognized are surface changes in which tight junctions and microvilli appear at the lateral margins of the cell near the medium. Later, changes in the distribution of intracellular organelles occur. The Golgi apparatus shifts towards the end of the cell facing the medium, and lysosomes shift toward the luminal end of the cell. The right junctions and microvilli at the luminal end of the cell disappear sometime after the cytoplasmic organelles rearrange. The luminal colloid disappears only after the surface changes (loss of tight junctions and microvilli) occur at the luminal end of the cell. There appears to be some regulation of the order in which changes occur during polarity reversal of the thyroid epithelial cell

A Mass-Loss Rate Determination For Zeta Puppis From The Quantitative Analysis Of X-Ray Emission-Line Profiles

We fit every emission line in the high-resolution Chandra grating spectrum of. Pup with an empirical line profile model that accounts for the effects of Doppler broadening and attenuation by the bulk wind. For each of 16 lines or line complexes that can be reliably measured, we determine a best-fitting fiducial optical depth, tau(*) equivalent to kappa(M) over dot/4 pi R(*)upsilon(infinity), and place confidence limits on this parameter. These 16 lines include seven that have not previously been reported on in the literature. The extended wavelength range of these lines allows us to infer, for the first time, a clear increase in tau(*) with line wavelength, as expected from the wavelength increase of bound-free absorption opacity. The small overall values of tau(*), reflected in the rather modest asymmetry in the line profiles, can moreover all be fitted simultaneously by simply assuming a moderate mass-loss rate of 3.5 +/- 0.3 x 10(-6) M(circle dot) yr(-1), without any need to invoke porosity effects in the wind. The quoted uncertainty is statistical, but the largest source of uncertainty in the derived mass-loss rate is due to the uncertainty in the elemental abundances of zeta Pup, which affects the continuum opacity of the wind, and which we estimate to be a factor of 2. Even so, the mass-loss rate we find is significantly below the most recent smooth-wind H alpha mass-loss rate determinations for zeta Pup, but is in line with newer determinations that account for small-scale wind clumping. If zeta Pup is representative of other massive stars, these results will have important implications for stellar and Galactic evolution

Chandra X-Ray Spectroscopy Of The Very Early O Supergiant HD 93129A: Constraints On Wind Shocks And The Mass-Loss Rate

We present an analysis of both the resolved X-ray emission-line profiles and the broad-band X-ray spectrum of the O-2 If* star HD 93129A, measured with the Chandra High Energy Transmission Grating Spectrometer ( HETGS). This star is among the earliest and most massive stars in the Galaxy, and provides a test of the embedded wind-shock scenario in a very dense and powerful wind. A major new result is that continuum absorption by the dense wind is the primary cause of the hardness of the observed X-ray spectrum, while intrinsically hard emission from colliding wind shocks contributes less than 10 per cent of the X-ray flux. We find results consistent with the predictions of numerical simulations of the line-driving instability, including line broadening indicating an onset radius of X-ray emission of several tenths of R-*. Helium-like forbidden-to-intercombination line ratios are consistent with this onset radius, and inconsistent with being formed in a wind-collision interface with the star\u27s closest visual companion at a distance of 100 au. The broad-band X-ray spectrum is fitted with a dominant emission temperature of just kT = 0.6 keV along with significant wind absorption. The broad-band wind absorption and the line profiles provide two independent measurements of the wind mass-loss rate:. M = 5.2(-1.5)(+1.8) x 10(-6) and 6.8(-2.2)(+2.8) x 10(-6) M-circle dot yr(-1), respectively. This is the first consistent modelling of the X-ray line-profile shapes and broad-band X-ray spectral energy distribution in a massive star, and represents a reduction of a factor of 3-4 compared to the standard H alpha mass-loss rate that assumes a smooth wind

Polarization Selection Rules and Superconducting Gap Anisotropy in Bi2Sr2CaCu2O8Bi_2Sr_2CaCu_2O_8

We discuss polarization selection rules for angle-resolved photoemission spectroscopy in Bi2212. Using these we show that the ``hump'' in the superconducting gap observed in the $X$ quadrant in our earlier work is not on the main $CuO_2$ band, but rather on an umklapp band arising from the structural superlattice. The intrinsic gap is most likely quite small over a range of $\pm 10^\circ$ about the diagonal directions.Comment: 3 pages, revtex, 3 uuencoded postscript figure

Asymmetric magnetic interference patterns in 0-pi Josephson junctions

We examine the magnetic interference patterns of Josephson junctions with a region of 0- and of pi-phase shift. Such junctions have recently been realized as c-axis YBCO-Pb junctions with a single twin boundary in YBCO. We show that in general the junction generates self-fields which introduces an asymmetry in the critical current under reversal of the magnetic field. Numerical calculations of these asymmetries indicate they account well for the unexplained features observed in single twin boundary junctions.Comment: 4 pages, 3 figure

Spontaneous Flux and Magnetic Interference Patterns in 0-pi Josephson Junctions

The spontaneous flux generation and magnetic field modulation of the critical current in a 0-pi Josephson junction are calculated for different ratios of the junction length to the Josephson penetration depth, and different ratios of the 0-junction length to the pi-junction length. These calculations apply to a Pb-YBCO c-axis oriented junction with one YBCO twin boundary, as well as other experimental systems. Measurements of such a junction can provide information on the nature of the c-axis Josephson coupling and the symmetry of the order parameter in YBCO. We find spontaneous flux even for very short symmetric 0-pi junctions, but asymmetric junctions have qualitatively different behavior.Comment: 13 pages, TEX,+ 7 figures, postscrip

C-axis Josephson Tunneling Between YBCO and Pb: Direct Evidence for Mixed Order Parameter Symmetry in a High-T_c Superconductor

We report a new class of $c$-axis Josephson tunneling experiments in which a conventional superconductor (Pb) is deposited across a single twin boundary of a YBa_2Cu_3O_{7-\delta} crystal. We measure the critical current as a function of magnitude and angle of magnetic field applied in the plane of the junction. In all samples, we observe a clear experimental signature of an order parameter phase shift across the twin boundary. These results provide strong evidence for mixed $d$- and $s$-wave pairing in YBCO, with a reversal in the sign of the $s$-wave component across the twin boundary.Comment: 4 pages RevTex, 4 postscript figures included, submitted to Phys. Rev. Let