The actin binding proteins cortactin and HS1 are dispensable for platelet actin nodule and megakaryocyte podosome formation

A dynamic, properly organised actin cytoskeleton is critical for the production and haemostatic function of platelets. The Wiskott Aldrich Syndrome protein (WASp) and Actin-Related Proteins 2 & 3 Complex (Arp2/3 complex) are critical mediators of actin polymerisation and organisation in many cell types. In platelets and megakaryocytes, these proteins have been shown to be important for proper platelet production and function. The cortactin family of proteins (Cttn & HS1) are known to regulate WASp-Arp2/3-mediated actin polymerisation in other cell types and so here we address the role of these proteins in platelets using knockout mouse models. We generated mice lacking Cttn and HS1 in the megakaryocyte/platelet lineage. These mice had normal platelet production, with platelet number, size and surface receptor profile comparable to controls. Platelet function was also unaffected by loss of Cttn/HS1 with no differences observed in a range of platelet function assays including aggregation, secretion, spreading, clot retraction or tyrosine phosphorylation. No effect on tail bleeding time or in thrombosis models was observed. In addition, platelet actin nodules, and megakaryocyte podosomes, actin-based structures known to be dependent on WASp and the Arp2/3 complex, formed normally. We conclude that despite the importance of WASp and the Arp2/3 complex in regulating F-actin dynamics in many cells types, the role of cortactin in their regulation appears to be fulfilled by other proteins in platelets

Avaliação dos impactos da Tecnologia Acasalamento de Outono em Bovinos de Corte.

Identificação da tecnologia; Procedimentos metodológicos; Identificação dos impactos na cadeia produtiva; Impactos econômicos; Impactos sociais; Impactos ambientais; Avaliação integrada e comparativa dos impactos gerados; Custos para a geração da tecnologia; Ações sociais.bitstream/item/64239/1/DT89.pd

Complementary stereotyping of ethnic minorities predicts system justification in Poland

We investigate the phenomenon of complementary stereotyping of ethnic minorities in Poland and its relationship to system justification. Using results from a nationally representative survey we test the hypothesis that complementary stereotypes—according to which ethnic minorities are seen as possessing distinctive, offsetting strengths and weaknesses—would be associated with system justification among Polish majority citizens. For four minorities, results indicated that stereotyping them as (a) low in morality but high in competence or (b) high in morality but low in competence predicted greater system justification. These results suggest that even in a context that is low in support for the status quo, complementary stereotyping of ethnic minorities is linked to system justification processes. For the three minority groups that were lowest in social status, complementary stereotyping was unrelated to system justification. It appears that negative attitude towards these groups can be expressed openly, regardless of one’s degree of system justification

The Effects of Sex-Role Attitudes and Group Composition on Men and Women in Groups

The dual impact of group gender composition and sex-role attitudes on self-perceptions and social behavior was explored. Androgynous and stereotyped men and women were placed in groups of skewed sex composition. Subjects\u27 self-descriptions of masculine attributes shifted significantly in the group environment. In some instances, sex role-stereotyped subjects responded most stereotypically when their gender was in the minority in the group. Differences between men and women and between androgynous and stereotyped subjects in sex role-related preferences for group roles and discussion topics were also found

Aquifer Vulnerability Mapping in Katsina-Ala Area, Central Nigeria Using Integrated Electrical Conductivity (IEC)

The present demand for water are not only to detect and develop new groundwater systems to meet up with the millennium development goals, but also to protect existing water reservoirs against contaminants. This research work is aimed at mapping out aquifer vulnerability by integrated electrical conductivity (IEC) in Katsina-Ala area, Central Nigeria. With Schlumberger configuration, Twenty-Six (26) vertical electrical soundings (VES) were occupied in the present study using Abem terrameter SAS 300c at the peak of dry season (January to March) from which the VES data collected was interpreted for geo-electrical parameters. The parameters estimated for overburden was used to calculate the IEC and was indexed to generate a vulnerability map of the study area. The indexes revealed three strong regimes which include vulnerable ( , highly vulnerable (IEC  and extremely vulnerable (IEC from 0 to 1240脳10-3siesmens)"> . The result will aid in the design of modalities for monitoring and protection of aquifer front against contaminants in the study area. Keywords: Aquifer, Integrated electrical conductivity, Overburden, Vulnerability, Katsina-Al

Transistor sizing analysis of regular fabrics

This paper presents an extensive transistor sizing analysis for regular transistor fabrics. Several evaluation methods have been exploited, such as DC simulations, ring oscillators and single-gate open chain structures. Different design aspects are addressed taking into account stacked transistors, cells with drive strengths and circuit critical paths. The performance degradation of using regular fabrics in comparison to standard cells is naturally expected, but it is quite important to evaluate the dimension of such impact. The results were obtained for predictive PTM45 CMOS parameters, and the conclusions can be easily extrapolated to other technology nodes and fabrication processesPostprint (published version