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Syk-dependent Phosphorylation of CLEC-2: A Novel Mechanism of Hem-Immunoreceptor Tyrosine-Based Activation Motif Signaling
The C-type lectin-like receptor CLEC-2 signals via phosphorylation of a single cytoplasmic YXXL sequence known as a hem-immunoreceptor tyrosine-based activation motif (hemITAM). In this study, we show that phosphorylation of CLEC-2 by the snake toxin rhodocytin is abolished in the absence of the tyrosine kinase Syk but is not altered in the absence of the major platelet Src family kinases, Fyn, Lyn, and Src, or the tyrosine phosphatase CD148, which regulates the basal activity of Src family kinases. Further, phosphorylation of CLEC-2 by rhodocytin is not altered in the presence of the Src family kinase inhibitor PP2, even though PLCγ2 phosphorylation and platelet activation are abolished. A similar dependence of phosphorylation of CLEC-2 on Syk is also seen in response to stimulation by an IgG mAb to CLEC-2, although interestingly CLEC-2 phosphorylation is also reduced in the absence of Lyn. These results provide the first definitive evidence that Syk mediates phosphorylation of the CLEC-2 hemITAM receptor with Src family kinases playing a critical role further downstream through the regulation of Syk and other effector proteins, providing a new paradigm in signaling by YXXL-containing receptors
Roles of Mitochondrial Dynamics under Stressful and Normal Conditions in Yeast Cells
Eukaryotic cells contain dynamic mitochondrial filaments: they fuse and divide. Here we summarize data on the protein machinery driving mitochondrial dynamics in yeast and also discuss the factors that affect the fusion-fission balance. Fission is a general stress response of cells, and in the case of yeast this response appears to be prosurvival. At the same time, even under normal conditions yeast mitochondria undergo continuous cycles of fusion and fission. This seems to be a futile cycle and also expensive from the energy point of view. Why does it exist? Benefits might be the same as in the case of sexual reproduction. Indeed, mixing and separating of mitochondrial content allows mitochondrial DNA to segregate and recombine randomly, leading to high variation in the numbers of mutations per individual mitochondrion. This opens a possibility for effective purifying selection-elimination of mitochondria highly contaminated by deleterious mutations. The beneficial action presumes a mechanism for removal of defective mitochondria. We argue that selective mitochondrial autophagy or asymmetrical distribution of mitochondria during cell division could be at the core of such mechanism
Phenomenon of Cloning and specificity of its usage
Cloning is studied by different branches of science. Medicine is interested in cloning because of its ability to transplant special tissues and organs, genetics - with the purpose of studying heredity and succession, sociology deals with moral and ethic aspects of the phenomenon. The paper is devoted to the study of cloning, its special features and usage in different spheres of social life. The article represents main types of cloning, specificity of vegetative and animal cloning and problems of its expansion. The paper also demonstrates the actual topic of nowadays studies connected with human cloning and its aftereffects for science and society. The article may be useful for a wide audience and for people, who are interested in studies of cloning and problems of its realization
Physical nature of critical wave functions in Fibonacci systems
We report on a new class of critical states in the energy spectrum of general
Fibonacci systems. By introducing a transfer matrix renormalization technique,
we prove that the charge distribution of these states spreads over the whole
system, showing transport properties characteristic of electronic extended
states. Our analytical method is a first step to find out the link between the
spatial structure of these critical wave functions and the quasiperiodic order
of the underlying lattice.Comment: REVTEX 3.0, 11 pages, 2 figures available upon request. To appear in
Phys. Rev. Let
Trace and antitrace maps for aperiodic sequences, their extensions and applications
We study aperiodic systems based on substitution rules by means of a
transfer-matrix approach. In addition to the well-known trace map, we
investigate the so-called `antitrace' map, which is the corresponding map for
the difference of the off-diagonal elements of the 2x2 transfer matrix. The
antitrace maps are obtained for various binary, ternary and quaternary
aperiodic sequences, such as the Fibonacci, Thue-Morse, period-doubling,
Rudin-Shapiro sequences, and certain generalizations. For arbitrary
substitution rules, we show that not only trace maps, but also antitrace maps
exist. The dimension of the our antitrace map is r(r+1)/2, where r denotes the
number of basic letters in the aperiodic sequence. Analogous maps for specific
matrix elements of the transfer matrix can also be constructed, but the maps
for the off-diagonal elements and for the difference of the diagonal elements
coincide with the antitrace map. Thus, from the trace and antitrace map, we can
determine any physical quantity related to the global transfer matrix of the
system. As examples, we employ these dynamical maps to compute the transmission
coefficients for optical multilayers, harmonic chains, and electronic systems.Comment: 13 pages, REVTeX, now also includes applications to electronic
systems, some references adde
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