Calaminus, C.

Fonslet, Jesper

Menegakis, A.

Napieczynska, H.

Pichler, B. J.

Severin, G. W.

Wiehr, S.

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017Imaging neuronal pathways with 52 Mn PET in ratsNapieczynska, H.; Severin, Gregory; Fonslet, Jesper; Menegakis, A.; Wiehr, S.; Pichler, B. J.; Calaminus,C.Published in:Journal of Cerebral Blood Flow and MetabolismPublication date:2017Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Napieczynska, H., Severin, G. W., Fonslet, J., Menegakis, A., Wiehr, S., Pichler, B. J., & Calaminus, C. (2017).Imaging neuronal pathways with 52 Mn PET in rats. Journal of Cerebral Blood Flow and Metabolism, 37(Suppl.1), 149. [PS01-083].PS01-083Poster Viewing Session IImaging neuronal pathways with 52MnPET in ratsH. Napieczynska1,2, G.W. Severin3,J. Fonslet3, A. Menegakis4, S. Wiehr1,B.J. Pichler1 and C. Calaminus11Eberhard Karls University Tuebingen, Werner Siemens ImagingCenter, Department of Preclinical Imaging and Radiopharmacy,Tuebingen, Germany2International Max Planck Research School for Cognitive andSystems Neuroscience, Tuebingen, Germany3Technical University of Denmark, The Hevesy Laboratory,Center for Nuclear Technologies, Roskilde, Denmark4Eberhard Karls University Tuebingen, Medical Faculty andUniversity Hospital, Department of Radiation Oncology,Tuebingen, GermanyAbstractObjectives: We aimed at applying 52Mn for PET imagingof neuronal pathways in rats, and testing potential toxicityof the tracer to the dopaminergic neurons.Methods: 52Mn was produced by proton irradiation ofnatCr. We have elaborated the purification procedure andthe tracer was prepared for intracerebral administration inNa ascorbate-buffered saline. Rats were stereotacticallyinjected into the ventral tegmental area (VTA) or striatum(STR) with app. 170kBq and scanned 24 h later. Behavioral(rotameter test) and histological (TH-staining) evaluationwas performed after 4 weeks. Another group of rats wasinjected with a reduced radioactivity dose and the sametoxicity evaluation took place at three time points. Theinfluence of the radiation dose on the DNA integritywas tested with gH2AX-staining in a separate experiment.Finally, gradual transport of 52Mn along the dopaminergicpathways was imaged continuously for 6 h in additionalanimals.Results: 52Mn transport along the mesolimbic and nigros-triatal pathways was clearly visible in PET images of theVTA-injected rats, as well as along the striatonigral tractsfollowing the intrastriatal administration. Quantitative ana-lysis of the PET data confirmed the tracer distribution inexpected brain regions. Impaired motor control and dopa-minergic lesion were found in some animals treated withthe high radioactivity dose. This effect was completelyabolished by reducing the dose to 20 5 kBq. Therewas also no increase in the double strand DNA breaksin the brain tissues treated with the lower dose as com-pared to the controls. Gradual 52Mn transport along themesolimbic pathway could be observed in dynamic PETimages.Conclusions: 52Mn traces neuronal pathways which canbe imaged with PET. The optimized experimental protocolprevents lesioning dopaminergic neurons and affecting therotation behavior up to 4 weeks post-injection in rats.Stages of the progressive movement of the tracer alongthe dopaminergic pathway can be visualize.PS01-084Poster Viewing Session I[18F]-Fluoromisonidazole PET/MRIimaging exhibits hypoxic-ischemic tissuearound the hematoma in experimentalintracerebral hemorrhageN. Go´mez-Lado1, C. Correa-Paz2,L. Garcı´a-Varela1, E. Lo´pez-Arias2,A´. Ruibal1, J. Castillo2, J. Pardo-Montero1,F. Campos2, P. Aguiar1 and T. Sobrino21Health Research Institute of Santiago de Compostela -Universidade de Santiago de Compostela, Molecular ImagingResearch Group - In Vivo Molecular Imaging and MedicalPhysics Group, Santiago de Compostela, Spain2Health Research Institute of Santiago de Compostela, ClinicalNeurosciences Research Laboratory, Santiago de Compostela,SpainAbstractObjective: Hematoma expansion is one of the factorsmore associated to poor outcome in intracerebral hemor-rhage (ICH) patients. In this regard, some studies havesuggested that an ‘‘ischemic penumbra’’ might arise whenthe hematoma has a large expansion. However, clinicalstudies are inconclusive. Therefore, our aim was tostudy the presence of hypoxic-ischemic tissue around thehematoma by means of [18F]-Fluoromisonidazole ([18F]-FMISO) PET/MRI in an experimental ICH model.Methods: We used SD rats (350–375 g). Rats were ran-domized into: 1) control group (SD normal rats;n¼ 3); 2)ICH group (SD rats subjected to collagenase-inducedICH;n¼ 6). [18F]-FMISO was intravenous administratedin both groups. [18F]-FMISO PET studies were performedat different post-ICH times within the hematoma expan-sion period: 18,24,42 and 48 hours. FMISO uptake wasAbstracts 149

Imaging neuronal pathways with 52 Mn PET in rats

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Imaging neuronal pathways with 52 Mn PET in rats

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