Longitudinal Variability in Hydrochemistry and Zooplankton Community of a Large River: A Lagrangian‐Based Approach

The variability in water quality and zooplankton community structure during downstream transport was investigated in the Po river (Italy) using for the first time a Lagrangian sampling approach. Two surveys were conducted, one in spring under relatively high discharge levels, and one at low flows in summer. Twelve stations along a 332‐km stretch of the river’s lowland reach and four major tributaries were sampled. A hydrodynamic modelling system was used to determine water transport time along the river, with a satisfying fit between simulated and observed discharge values. No clear downstream trend in phosphorus and nitrogen concentrations was found. Conversely, a marked longitudinal decrease in dissolved silica supports the hypothesis of increasing downstream silica limitation during the phytoplankton growing season.In spring, at low residence time, no apparent plankton growth was observed during downstream transport. In summer, higher temperatures and lower turbulence and turbidity associated with longer residence time stimulated algal growth and in‐stream reproduction of fast‐growing rotifer taxa, with the gradual downstream development of a truly potamal assemblage and the increase of the ratio of euplanktonic to littoral/epibenthic rotifer taxa. Crustacean zooplankton density was generally low. The importance of biotic interactions within the zooplankton in driving community abundance and composition appeared to increase in the downstream direction, paralleled by a decrease in the influence of physical forcing. Tributary influence was especially evident where severe anthropogenic alterations of river hydrology and trophic status resulted in enhanced plankton growth, ultimately affecting zooplankton structure in the main river. Copyright © 2016 John Wiley & Sons, Ltd.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/134064/1/rra3028.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/134064/2/rra3028_am.pd

Gamma-ray observations of Cygnus X-1 above 100 MeV in the hard and soft states

We present the results of multi-year gamma-ray observations by the AGILE satellite of the black hole binary system Cygnus X-1. In a previous investigation we focused on gamma-ray observations of Cygnus X-1 in the hard state during the period mid-2007/2009. Here we present the results of the gamma-ray monitoring of Cygnus X-1 during the period 2010/mid-2012 carried out for which includes a remarkably prolonged `soft state' phase (June 2010 -- May 2011). Previous 1--10 MeV observations of Cyg X-1 in this state hinted at a possible existence of a non-thermal particle component with substantial modifications of the Comptonized emission from the inner accretion disk. Our AGILE data, averaged over the mid-2010/mid-2011 soft state of Cygnus X-1, provide a significant upper limit for gamma-ray emission above 100 MeV of F_soft < 20 x 10^{-8} ph/cm^2/s, excluding the existence of prominent non-thermal emission above 100 MeV during the soft state of Cygnus X-1. We discuss theoretical implications of our findings in the context of high-energy emission models of black hole accretion. We also discuss possible gamma-ray flares detected by AGILE. In addition to a previously reported episode observed by AGILE in October 2009 during the hard state, we report a weak but important candidate for enhanced emission which occurred at the end of June 2010 (2010-06-30 10:00 - 2010-07-02 10:00 UT) exactly in coincidence with a hard-to-soft state transition and before an anomalous radio flare. An appendix summarizes all previous high-energy observations and possible detections of Cygnus X-1 above 1 MeV.Comment: 16 pages, 12 figures, 1 table, accepted for publication in Ap

Chronic oleoylethanolamide treatment decreases hepatic triacylglycerol level in rat liver by a pparγ/srebp-mediated suppression of fatty acid and triacylglycerol synthesis

none11noOleoylethanolamide (OEA) is a naturally occurring bioactive lipid belonging to the family of N-acylethanolamides. A variety of beneficial effects have been attributed to OEA, although the greater interest is due to its potential role in the treatment of obesity, fatty liver, and eating-related disorders. To better clarify the mechanism of the antiadipogenic effect of OEA in the liver, using a lipidomic study performed by1H-NMR, LC-MS/MS and thin-layer chromatography analyses we evaluated the whole lipid composition of rat liver, following a two-week daily treatment of OEA (10 mg kg−1 i.p.). We found that OEA induced a significant reduction in hepatic triacylglycerol (TAG) content and significant changes in sphingolipid composition and ceramidase activity. We associated the antiadipogenic effect of OEA to decreased activity and expression of key enzymes involved in fatty acid and TAG syntheses, such as acetyl-CoA carboxylase, fatty acid synthase, diacylglycerol acyltransferase, and stearoyl-CoA desaturase 1. Moreover, we found that both SREBP-1 and PPARγ protein expression were significantly reduced in the liver of OEA-treated rats. Our findings add significant and important insights into the molecular mechanism of OEA on hepatic adipogenesis, and suggest a possible link between the OEA-induced changes in sphingolipid metabolism and suppression of hepatic TAG level.openRomano A.; Friuli M.; Del Coco L.; Longo S.; Vergara D.; Del Boccio P.; Valentinuzzi S.; Cicalini I.; Fanizzi F.P.; Gaetani S.; Giudetti A.M.Romano, A.; Friuli, M.; Del Coco, L.; Longo, S.; Vergara, D.; Del Boccio, P.; Valentinuzzi, S.; Cicalini, I.; Fanizzi, F. P.; Gaetani, S.; Giudetti, A. M

Molecular dynamics study of Eu3+ in an aqueous solution: Luminescence spectrum from simulated environments

Because doping ions are mainly surrounded by solvent molecules in the early stages of sol—gel processing, the luminescence of rare-earth ions in such an environment is known to be very similar to that observed in aqueous solutions. Thus we performed a molecular dynamics simulation of the trivalent europium in aqueous solution in order to produce a statistical set of environments and to calculate the spectroscopic features thereof. From radial and angular ordering studies, the hydration number of Eu3+ was found to be between eight and nine. The eight-hydrated configurations are nevertheless predominant and present a specific arrangement, according to the geometry of a square antiprism polyhedron. From the whole set of simulated configurations, we computed the ligand field parameters without considering any a priori symmetry. We then calculated the energy splitting of each J manifold and the transition probabilities from the 5D0 emitting state. A simulated luminescent spectrum was finally obtained and compared with the experimental sectrum. In addition to overall good agreement, we were able to analyse the 5D0 → 7F1 transition shape, which revealed a sensitive dependence on the local structure, and especially on the hydration number

On the Angular Resolution of the AGILE gamma-ray imaging detector

We present a study of the Angular Resolution of the AGILE gamma-ray imaging detector (GRID) that is operational in space since April 2007. The AGILE instrument is made of an array of 12 planes each equipped with a Tungsten converter and Silicon micros trip detectors and is sensitive in the energy range 50 MeV - 10 GeV. Among the space instruments devoted to gamma-ray astrophysics, AGILE uniquely exploits an analog readout system with dedicated electronics coupled with Silicon detectors. We show the results of Monte Carlo simulations carried out to reproduce the gamma-ray detection by the GRID, and we compare them to in-flight data. We use the Crab (pulsar + Nebula) system for discussion of real data performance, since its E^{-2} energy spectrum is representative of the majority of gamma-ray sources. For Crab-like spectrum sources, the GRID angular resolution (FWHM of ~4deg at 100 MeV; ~0.8deg at 1 GeV; ~0.9deg integrating the full energy band from 100 MeV to tens of GeV) is stable across a large field of view, being characterized by a flat response up to 30deg off-axis. A comparison of the angular resolution obtained by the two operational gamma-ray instruments, AGILE-GRID and Fermi-LAT, is interesting in view of future gamma-ray missions, that are currently under study. The two instruments exploit different detector configurations affecting the angular resolution: the former being optimized in the readout and track reconstruction especially in the low-energy band, the latter in terms of converter thickness and power consumption. We show that, despite these differences, the angular resolution of both instruments is very similar between 100 MeV and a few GeV.Comment: 19 pages, 8 figures, accepted for publication in Ap

AGILE Observations of the Gravitational Wave Event GW150914

We report the results of an extensive search in the AGILE data for a gamma-ray counterpart of the LIGO gravitational wave event GW150914. Currently in spinning mode, AGILE has the potential of covering with its gamma-ray instrument 80 % of the sky more than 100 times a day. It turns out that AGILE came within a minute from the event time of observing the accessible GW150914 localization region. Interestingly, the gamma-ray detector exposed about 65 % of this region during the 100 s time intervals centered at -100 s and +300 s from the event time. We determine a 2-sigma flux upper limit in the band 50 MeV - 10 GeV, $UL = 1.9 \times 10^{-8} \rm \, erg \, cm^{-2} \, s^{-1}$ obtained about 300 s after the event. The timing of this measurement is the fastest ever obtained for GW150914, and significantly constrains the electromagnetic emission of a possible high-energy counterpart. We also carried out a search for a gamma-ray precursor and delayed emission over timescales ranging from minutes to days: in particular, we obtained an optimal exposure during the interval -150 / -30 s. In all these observations, we do not detect a significant signal associated with GW150914. We do not reveal the weak transient source reported by Fermi-GBM 0.4 s after the event time. However, even though a gamma-ray counterpart of the GW150914 event was not detected, the prospects for future AGILE observations of gravitational wave sources are decidedly promising.Comment: 20 pages, 6 figures. Submitted to the Astrophysical Journal Letters on April 1, 201

Regulatory T cells with multiple suppressive and potentially pro-tumor activities accumulate in human colorectal cancer

Tregs can contribute to tumor progression by suppressing antitumor immunity. Exceptionally, in human colorectal cancer (CRC), Tregs are thought to exert beneficial roles in controlling pro-tumor chronic inflammation. The goal of our study was to characterize CRC-infiltrating Tregs at multiple levels, by phenotypical, molecular and functional evaluation of Tregs from the tumor site, compared to non-tumoral mucosa and peripheral blood of CRC patients. The frequency of Tregs was higher in mucosa than in blood, and further significantly increased in tumor. Ex vivo, those Tregs suppressed the proliferation of tumor-infiltrating CD8(+) and CD4(+) T cells. A differential compartmentalization was detected between Helioshigh and Helios(low) Treg subsets (thymus-derived versus peripherally induced): while Helios(low) Tregs were enriched in both sites, only Helios(high) Tregs accumulated significantly and specifically in tumors, displayed a highly demethylated TSDR region and contained high proportions of cells expressing CD39 and OX40, markers of activation and suppression. Besides the suppression of T cells, Tregs may contribute to CRC progression also through releasing IL-17, or differentiating into Tfr cells that potentially antagonize a protective Tfh response, events that were both detected in tumor-associated Tregs. Overall, our data indicate that Treg accumulation may contribute through multiple mechanisms to CRC establishment and progression

Neutral pion emission from accelerated protons in the supernova remnant W44

We present the AGILE gamma-ray observations in the energy range 50 MeV - 10 GeV of the supernova remnant (SNR) W44, one of the most interesting systems for studying cosmic-ray production. W44 is an intermediate-age SNR (20, 000 years) and its ejecta expand in a dense medium as shown by a prominent radio shell, nearby molecular clouds, and bright [SII] emitting regions. We extend our gamma-ray analysis to energies substantially lower than previous measurements which could not conclusively establish the nature of the radiation. We find that gamma-ray emission matches remarkably well both the position and shape of the inner SNR shocked plasma. Furthermore, the gamma-ray spectrum shows a prominent peak near 1 GeV with a clear decrement at energies below a few hundreds of MeV as expected from neutral pion decay. Here we demonstrate that: (1) hadron-dominated models are consistent with all W44 multiwavelength constraints derived from radio, optical, X-ray, and gamma-ray observations; (2) ad hoc lepton-dominated models fail to explain simultaneously the well-constrained gamma-ray and radio spectra, and require a circumstellar density much larger than the value derived from observations; (3) the hadron energy spectrum is well described by a power-law (with index s = 3.0 \pm 0.1) and a low-energy cut-off at Ec = 6 \pm 1 GeV. Direct evidence for pion emission is then established in an SNR for the first time.Comment: accepted for publication on ApJ

Agile Detection of Delayed Gamma-Ray Emission from the Short Gamma-Ray Burst GRB 090510

Short gamma-ray bursts (GRBs), typically lasting less than 2 s, are a special class of GRBs of great interest. We report the detection by the AGILE satellite of the short GRB 090510 which shows two clearly distinct emission phases: a prompt phase lasting ~ 200 msec and a second phase lasting tens of seconds. The prompt phase is relatively intense in the 0.3-10 MeV range with a spectrum characterized by a large peak/cutoff energy near 3 MeV, in this phase, no significant high-energy gamma-ray emission is detected. At the end of the prompt phase, intense gamma-ray emission above 30 MeV is detected showing a power-law time decay of the flux of the type t^-1.3 and a broad-band spectrum remarkably different from that of the prompt phase. It extends from sub-MeV to hundreds of MeV energies with a photon index alpha ~ 1.5. GRB 090510 provides the first case of a short GRB with delayed gamma-ray emission. We present the timing and spectral data of GRB 090510 and briefly discuss its remarkable properties within the current models of gamma-ray emission of short GRBs.Comment: Accepted by the Astrophysical Journal Letters on September 11, 200