Supplementary material for the article: Kop, T.; Bjelaković, M.; Dordević, J.; Žekić, A.; Milić, D. Fulleropyrrolidines Derived from Dioxa- and Trioxaalkyl-Tethered Diglycines. RSC Advances 2015, 5 (115), 94599–94606. https://doi.org/10.1039/c5ra17392b

Supplementary material for: [https://doi.org/10.1039/c5ra17392b]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1998

Prediction of metabolisable energy of poultry feeds by estimating in vitro organic matter digestibility

The aim of this study was to develop equations to predict the in vivo apparent metabolisable energy (AME) of poultry feeds using an in vitro method for estimation of organic matter digestibility. In this study, a total of 57 samples of feedstuffs and 23 samples complete diets for poultry were used. Dry matter (DM), crude protein (CP), crude fibre (CF), crude fat (CFat) and crude ash (CA) of the diets were determined. A modified method for estimating the enzymatic digestibility of organic matter (EDOM) was used. For the determination of in vivo ME, the rooster digestibility assay was followed. Obtained laboratory results, that is in vitro and proximate analysis values were regressed against the in vivo ME values and equations for predicting the in vivo ME of feeds for poultry have been derived. Using CA, CF, CFat and in vitro EDOM as predictors, the following equation for predicting the in vivo ME in poultry feeds was derived: ME (MJ/kg DM) = 5.46 – 0.2166 x CA – 0.0946 x CF + 0.2219 x CFat + 0.1054 x EDOM (R2 = 0.844, RSD = 1.10). Using only EDOM as predictor generated the equation: ME (MJ/kg DM) = -0.41 + 0.1769 x EDOM (R2 = 0.689; RSD = 1.63). Results show that using only EDOM as a predictor was not as accurate as when the other variables were included.Key words: Metabolisable energy, prediction, poultry, feeds, organic matter digestibilit

Supplementary material for the article: Kop, T.; Bjelaković, M.; Dordević, J.; Žekić, A.; Milić, D. Fulleropyrrolidines Derived from Dioxa- and Trioxaalkyl-Tethered Diglycines. RSC Advances 2015, 5 (115), 94599–94606. https://doi.org/10.1039/c5ra17392b

Supplementary material for: [https://doi.org/10.1039/c5ra17392b]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/1998

Fulleropyrrolidines derived from dioxa- and trioxaalkyl-tethered diglycines

Two different alpha,omega-diglicynes linked by linear polyoxaalkyl chains in the presence of formaldehyde underwent Prato reaction to the fullerene C-60. The shorter linker templated formation of only cis-bisadducts, while the longer one afforded a mixture of four bisadducts (all cis and the equatorial) and difullerene dumbbell compound. Their structures were confirmed by the extensive analysis of the spectral data and molecular symmetry, as well. All compounds expressed an ability to arrange into hierarchically ordered supramolecular aggregates, the form of which depended both on the addition pattern and the spacer structure. The attenuated electron-accepting affinity, examined by cyclic voltammetry was in agreement with diminished delocalization of the pi-electronic system. In addition, all compounds exerted a notable radical scavenging activity.Supplementary material: [http://cherry.chem.bg.ac.rs/handle/123456789/3370

Electrochemical, theoretical, and morphological studies of antioxidant fullerosteroids

Four fullerosteroidal conjugates, previously confirmed to express two- to threefold better antioxidant activity in vitro than C-60, were subjected to additional studies, including electrochemical, theoretical, and morphological examination. All tested compounds underwent reversible, diffusion-controlled reductions. A notable influence of the solvent properties on the reduction potential, the level of aggregation, and the lowest unoccupied molecular orbital (LUMO) energy was observed. Theoretical calculations indicated that the energy gain obtained by an intermediate formation, together with compounds' polarizability, polarity, and lipophilicity contributed to the radical quenching capacity. Very large supramolecular aggregates of all fullerosteroidal esters with no hierarchical arrangement were observed in precipitated samples, while solvent induced self-assembling led to round nanoplates, which further arranged to flower-shaped hierarchically ordered architectures or uniformly distributed discoid particles. As in electrochemical studies, fine tuning of the aggregation level was achieved by the solvent