Lack of increased availability of root-derived C may explain the low N2O emission from low N-urine patches

Urine deposition on grassland causes significant N2O losses, which in some cases may result from increased denitrification stimulated by labile compounds released from scorched plant roots. Two 12-day experiments were conducted in 13C-labelled grassland monoliths to investigate the link between N2O production and carbon mineralization following application of low rates of urine-N. Measurements of N2O and CO2 emissions from the monoliths as well as δ13C signal of evolved CO2 were done on day -4, -1, 0, 1, 2, 4, 5, 6 and 7 after application of urine corresponding to 3.1 and 5.5 g N m-2 in the first and second experiment, respectively. The δ13C signal was also determined for soil organic matter, dissolved organic C and CO2 evolved by microbial respiration. In addition, denitrifying enzyme activity (DEA) and nitrifying enzyme activity (NEA) were measured on day -1, 2 and 7 after the first urine application event. Urine did not affect DEA, whereas NEA was enhanced 2 days after urine application. In the first experiment, urine had no significant effect on the N2O flux, which was generally low (-8 to 14 μg N2O-N m-2 h-1). After the second application event, the N2O emission increased significantly to 87 μg N2O-N m-2 h-1 and the N2O emission factor for the added urine-N was 0.18 %. However, the associated 13C signal of soil respiration was unaffected by urine. Consequently, the increased N2O emission from the simulated low N-urine patches was not caused by enhanced denitrification stimulated by labile compounds released from scorched plant roots

Prediction of the derivative discontinuity in density functional theory from an electrostatic description of the exchange and correlation potential

We propose a new approach to approximate the exchange and correlation (XC) functional in density functional theory. The XC potential is considered as an electrostatic potential, generated by a fictitious XC density, which is in turn a functional of the electronic density. We apply the approach to develop a correction scheme that fixes the asymptotic behavior of any approximated XC potential for finite systems. Additionally, the correction procedure gives the value of the derivative discontinuity; therefore it can directly predict the fundamental gap as a ground-state property.Comment: 5 pages, 4 figure

A dynamic mode of mitotic bookmarking by transcription factors.

During mitosis, transcription is shut off, chromatin condenses, and most transcription factors (TFs) are reported to be excluded from chromosomes. How do daughter cells re-establish the original transcription program? Recent discoveries that a select set of TFs remain bound on mitotic chromosomes suggest a potential mechanism for maintaining transcriptional programs through the cell cycle termed mitotic bookmarking. Here we report instead that many TFs remain associated with chromosomes in mouse embryonic stem cells, and that the exclusion previously described is largely a fixation artifact. In particular, most TFs we tested are significantly enriched on mitotic chromosomes. Studies with Sox2 reveal that this mitotic interaction is more dynamic than in interphase and is facilitated by both DNA binding and nuclear import. Furthermore, this dynamic mode results from lack of transcriptional activation rather than decreased accessibility of underlying DNA sequences in mitosis. The nature of the cross-linking artifact prompts careful re-examination of the role of TFs in mitotic bookmarking

A simple rule for the evolution of fast dispersal at the edge of expanding populations

Evolution by natural selection is commonly perceived as a process that favors those that replicate faster to leave more offspring; nature, however, seem to abound with examples where organisms forgo some replicative potential to disperse faster. When does selection favor invasion of the fastest? Motivated by evolution experiments with swarming bacteria we searched for a simple rule. In experiments, a fast hyperswarmer mutant that pays a reproductive cost to make many copies of its flagellum invades a population of mono-flagellated bacteria by reaching the expanding population edge; a two-species mathematical model explains that invasion of the edge occurs only if the invasive species' expansion rate, v₂, which results from the combination of the species growth rate and its dispersal speed (but not its carrying capacity), exceeds the established species', v₁. The simple rule that we derive, v₂ > v₁, appears to be general: less favorable initial conditions, such as smaller initial sizes and longer distances to the population edge, delay but do not entirely prevent invasion. Despite intricacies of the swarming system, experimental tests agree well with model predictions suggesting that the general theory should apply to other expanding populations with trade-offs between growth and dispersal, including non-native invasive species and cancer metastases.First author draf

Microlensing by Cosmic Strings

We consider the signature and detectability of gravitational microlensing of distant quasars by cosmic strings. Because of the simple image configuration such events will have a characteristic light curve, in which a source would appear to brighten by exactly a factor of two, before reverting to its original apparent brightness. We calculate the optical depth and event rate, and conclude that current predictions and limits on the total length of strings on the sky imply optical depths of \la 10^{-8} and event rates of fewer than one event per $10^9$ sources per year. Disregarding those predictions but replacing them with limits on the density of cosmic strings from the CMB fluctuation spectrum, leaves only a small region of parameter space (in which the sky contains about $3\times10^5$ strings with deficit angle of order 0.3 milli-arcseconds) for which a microlensing survey of exposure $10^7$ source-years, spanning a 20--40-year period, might reveal the presence of cosmic strings.Comment: 4 pages, accepted for publication in MNRA