Insilico analysis of Arabidopsis ferric reductase oxidases (FRO) proteins associated with iron homeostasis

The ferric reduction oxidase (FRO) gene family is involved in various biological processes of plants and plays an essential role in metal homeostasis, tolerance, and signaling networks in response to several abiotic stresses. Our study describes the structural, functional characterization, and evolutionary relationships of eight Arabidopsis FRO proteins. The studies predicted the subcellular localization of FRO proteins to the plasma membrane, mitochondria, and chloroplast organelles. The structural analysis revealed localization of proteins onto the first and fifth chromosomes having 8-9exons and 8-10 transmembrane helices. The protein features of FRO proteins revealed 699-747 amino acids having 79600.02-84126.3 (Da) molecular weight. The six highly conserved protein motifs were predicted with 45-50 amino acids long representing ferric chelate reductase family domains. The phylogeny tree constructed using Clustal W divided the FRO proteins into two clusters and the interactome network revealed the co-expression of COPT1, NRAMP1, NRAMP3, NRAMP4, FRD3, OPT3, IRT1, IRT2, ZIF1, PYE proteins along with the seven FRO proteins

Assessment of Genetic Variation and Character Association among Yield and Yield Attributing Traits in Sweet Corn (Zea mays L. saccharata) Inbred Lines

In this study, forty-six sweet corn inbred lines were evaluated to assess the variability and association among 11 yield and yield attributing traits at College Farm, College of Agriculture, Rajendranagar, PJTSAU. The traits plant height, ear height, cob length, cob girth, number of rows per ear, number of kernels per row, cob weight with husk and cob weight without husk have shown moderate to high range of PCV, GCV, heritability and genetic advance over mean displaying the importance of these traits in selecting superior sweet corn inbreds. Critical analysis of results obtained from correlation and path analysis revealed that the traits cob length, cob girth, number of kernel rows per ear and cob weight without husk posses high positive significant associations and positive direct effects. From this study it was concluded that cob length, cob girth, number of kernel rows per ear and cob weight without husk are the key traits to be given prime importance during selection for further improvement of production as they exhibited significant positive direct effects with cob weight with husk

Studies On Spatio-Temporal and Phytohormonal Expression Pattern of MTP2 Protein and its Characterization across Different Plant Species

Zinc is an essential micronutrient involved in a wide range of physiological processes of plants (Marschner, 1993). Even though it is required for plantgrowth, the excess amount of Zn causes toxic effects to plants. It is known that during Fe deficiency, the uptake of Zn is induced which leads to the accumulation of the excess amount of Zn in the plant cytoplasm. The plant has evolved an efficient mechanism in detoxifying the excess Zn uptake during Fe deficiency conditions, of which vacuoles are identified to be the main storage and detoxifying organelles for Zn excess in the cytoplasm (Gustin et al., 2009). Zinc is normally available to the plants in its oxidized form Zn2+ from the soil through the mass flow and diffusion mechanism by roots (Rattan and Deb, 1981). Availability of Zn is high in low acidic pH wherein the high pH reduces its availability in soil and the root plasma membrane transporters reported for major uptake of Zn from the soil (Marschner, 1993)

Rainbow refractrometry: On the validity domain of Airy's and Nussenzveig's theories

cited By 33International audienceno abstrac

Assessment of Various Variability Parameters and Correlation of Quantitative Characters in Maize (Zea mays L.) Inbred Lines

The present research problem was carried out to assess the variability parameters, heritability, genetic advance and correlations among various quantitative traits in maize inbred lines. A total of 69 inbred lines were evaluated in alpha lattice design with 2 replications at Winter Nursery Centre, Hyderabad. ANOVA results displays genotypes were significantly different from each other. From the results it was depicted that Phenotypic Coefficient of Variation (PCV) was higher than Genotypic Coefficient of Variation (GCV) indicating the influence of environment. The traits, No. of kernels per row, Cob weight, Grain yield per cob showed high GCV and PCV values likewise Days to 50% tasseling, Days to 50% silking and Days to 75% dry husk showed low GCV and PCV values. Most of the characters showed high heritability and traits such as Plant height, Tassel length, Ear length, No. of kernels per row, Cob weight and Grain yield per cob showed high GAM and its values ranged from 21.5% to 101.30%. So, the traits such as No. of kernels per row, Cob weight and Grain yield per cob can be used for further crop improvement in the breeding programme. Of all the traits under study except, Days to 50% tasseling, Days to 50% silking and Days to 75% dry husk showed positive significant correlation with grain yield per cob indicating selection for the traits will enhance the grain yield whereas the excepted traits showed negative significant association with grain yield per cob indicating selection for these traits is also essential as it reduces duration of crop

Assessment of rickettsial diseases presenting as acute undifferentiated febrile illness using clinical tools in a government tertiary care teaching hospital, Chamarajanagar, Karnataka

Background: Rickettsial diseases are re-emerging in India. Acute undifferentiated febrile illness is a difficult case to treat (AUFI). The greatest problem for clinicians is the early diagnosis of these diseases when antibiotic therapy is most successful.To determine the prevalence of RDs, therapy outcomes, and socio-demographic factors associated with RDs in AUFI. Patients and Methods: Fever and/or clinical signs of Rickettsial infection in patients admitted to the CIMS teaching hospital in Chamarajanagar for four years. Purposive sampling found AUFI in 1638 people. The diagnosis of rickettsial disease relied on clinical features. The Weil-Felix test was one. Then came a 48–72-hour estimate of doxycycline response. Results:Out of 1810 AUFI cases, 198 (10.93%) were rickettsial, with 190 (95.95%) having an RGA score of 14 or more, and 18 (9.09%) having an RGA score of 14 or less. Males aged 30-40 were most affected. Leucopenia, thrombocytopenia, etc. were found. Doxycycline was effective in 151 (89.35%) of cases within one week of onset. The remaining 18 (10.65%) required longer-term doxycycline with other drugs, three had severe complications, and one died. RD prevalence 70.01 %. Enteric fever, Dengue fever, Chikungunya fever, and Rickettsial diseases were the most common diseases diagnosed in patients with undifferentiated febrile illness.&nbsp

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Not AvailableCastor is an industrially important oilseed crop, which provides raw material to many industries. Despite its industrial importance, the genomic resources available for molecular breeding applications are limited. In the present study, a set of 135 SNP loci polymorphic between two castor inbred lines namely, JC-12 and RG-1963 were targeted to develop competitive allele specific PCR assays for genotyping purposes. The assays were validated in the F2 population of JC-12 × RG-1963 cross. Out of 135 assays designed, 124 were found to be successful in discriminating three expected genotypes in the F2 population. Using the SNP genotyping data, a genetic linkage map representing 10 haploid chromosomes of castor was constructed, which corresponded very well with the physical map. This set of validated SNP markers is a useful resource for application in molecular breeding of castor.ICA

Rainbow refractrometry: On the validity domain of Airy's and Nussenzveig's theories

cited By 33International audienceno abstrac