Trust Based Participant Driven Privacy Control in Participatory Sensing

Widespread use of sensors and multisensory personal devices generate a lot of personal information. Sharing this information with others could help in various ways. However, this information may be misused when shared with all. Sharing of information between trusted parties overcomes this problem. This paper describes a model to share information based on interactions and opinions to build trust among peers. It also considers institutional and other controls, which influence the behaviour of the peers. The trust and control build confidence. The computed confidence bespeaks whether to reveal information or not thereby increasing trusted cooperation among peers.Comment: 14 page

Reducing Interconnect Cost in NoC through Serialized Asynchronous Links

This work investigates the application of serialization as a means of reducing the number of wires in NoC combined with asynchronous links in order to simplify the clocking of the link. Throughput is reduced but savings in routing area and reduction in power could make this attractiv

A STUDY ON THE BIOACTIVE POTENTIAL OF FRESH AND DRIED SPROUTS OF COCOS NUCIFERA L.–AN IN VITRO AND IN-SILICO APPROACH

Objective: The main objective of the study is to analyze the medicinal properties, by giving scientific validation to the existing bioactive compounds present in the coconut sprouts and recommending the coconut sprouts as a natural product for the treatment of bacterial infection leading to inflammations.Methods: Phytochemical screening by qualitative and quantitative analysis of the primary and secondary constituents were carried out in aqueous and methanol extracts of the coconut sprouts (fresh and dried) using standard procedures. The phytochemicals were characterized using FT-IR, TLC and GC-MS analysis. Antibacterial activity was studied against several human pathogens by agar well diffusion method. Antioxidant activity using Reducing power, Hydrogen peroxide scavenging and DPPH radical scavenging methods and Anti-inflammatory activity through protein denaturation method were carried out. Further confirmation of the functional role of the phytoconstituent through in-silico docking was studied.Results: The qualitative phytochemical screening revealed the presence of essential phytoconstituents. The quantitative analysis revealed the presence of maximum Carbohydrates (0.60±0.1 mg/g), Proteins (39±0.8 mg/ml), Flavonoids (0.28±0.1 mg QE/g) and Terpenoids (95±1.2 mg/g) in methanolic fresh coconut sprout extract with respective standards. Maximum zone of inhibitions were shown by Shigella flexneri, Salmonella typhi and Klebsiella pneumoniae. Antioxidant and anti-inflammatory studies proved the presence of therapeutic bioactive potential in the sample. Through in-silico analysis, docking studies were performed to confirm the functional role of the specific therapeutic phytochemical.Conclusion: The fresh coconut sprouts are natural, economically potent food source for human health and can be a nutrient supplement with cost effective approach. The dried sprouts can also be recommended to the food industry for the large-scale production of nutrient-based foods with a quality check

BIOINFORMATIC STUDY OF AN ANTITUMOR PROTEIN, AZURIN

Objective: The main objective of this study is to analyze the structure and function of an antitumor protein, azurin, thereby giving validation to the protein structure and existing physicochemical properties in the anticancer protein which are responsible for the anticancer activity.Methods: Protein sequence analysis was done using Basic Local Alignment Search Tool (BLAST) with ten different randomly selected species of Pseudomonas obtained from GenBank. The physicochemical properties, prediction of secondary structure, identification of motifs and domains, three-dimensional (3-D) structure of the antitumor protein, validation through Ramachandran plot, multiple sequence alignment (MSA), and phylogenetic analysis were studied and functional property was confirmed through in silico docking.Results: The similarity search (BLAST-P analysis) for the primary sequence from GenBank carried out showed 86% similarity to the second sequence, azurin (Pseudomonas nitroreducens). The ProtParam, ExPASy tool server indicated the presence of essential physicochemical properties in azurin. Secondary structure prediction revealed random coil, extended strand, alpha helix, and beta turn. The study on domains indicated the presence of one domain in azurin responsible for the anticancer activity. The 3-D structural analysis revealed azurin as metalloprotein, of length-128, and polymer-1 with α-helices, β-sheets, and β-barrels. The validation carried out through Ramachandran plot showed the presence of two outliers (phi and psi). The biological relationship between the input sequences was studied through MSA and phylogenetic analysis. Further, azurin docked against the target protein (p53 tumor suppressor) showed the maximum binding affinity confirming its functional property of causing apoptosis.Conclusion: All the properties analyzed in the present study revealed that the azurin protein can act as a very good anticancer agent, and through the phylogenetic analysis, it was identified that Pseudomonas nitroreducens was closely related to the test organism Pseudomonas aeruginosa

Physical interaction between MYCN oncogene and polycomb repressive complex 2 (PRC2) in neuroblastoma: Functional and therapeutic implications

This article is made available through the Brunel Open Access Publishing Fund. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.CLU (clusterin) is a tumor suppressor gene that we have previously shown to be negatively modulated by the MYCN proto-oncogene, but the mechanism of repression was unclear. Here, we show that MYCN inhibits the expression of CLU by direct interaction with the non-canonical E box sequence CACGCG in the 5′-flanking region. Binding of MYCN to the CLU gene induces bivalent epigenetic marks and recruitment of repressive proteins such as histone deacetylases and Polycomb members. MYCN physically binds in vitro and in vivo to EZH2, a component of the Polycomb repressive complex 2, required to repress CLU. Notably, EZH2 interacts with the Myc box domain 3, a segment of MYC known to be essential for its transforming effects. The expression of CLU can be restored in MYCN-amplified cells by epigenetic drugs with therapeutic results. Importantly, the anticancer effects of the drugs are ablated if CLU expression is blunted by RNA interference. Our study implies that MYC tumorigenesis can be effectively antagonized by epigenetic drugs that interfere with the recruitment of chromatin modifiers at repressive E boxes of tumor suppressor genes such as CLU.SPARKS, The Neuroblastoma Society, a Wellcome Trust grant (to A. S.), and the Italian Association for Cancer Research

Tree leaf meal from fodder trees in silvipasture and their potential to support growth in young ruminants

The study was conducted with the aim of utilizing leaf meal prepared from Leucaena leucocephala and Gliricidia sepium trees from silvipasture model of agroforestry as alternate feeding strategy on partial replacement of feed ingredients in compound feeds of goat kids and buffalo calves. The edible leaf biomass from Leucaena leucocephala and Gliricidia sepium was 9.20 and 18.54 MT/ha/year respectively and their respective crude protein content was 14.81 and 17.66 per cent. Two feeding trials were conducted with Leucaena leucocephala and Gliricidia sepium leaf meal mix (1:1 ratio) incorporated in concentrate feed for goat kids and buffalo calves at 30 per cent inclusion. The average daily gain of goat kids and buffalo calves fed tree leaf meal incorporated concentrate feed was comparable to daily gain of kids / calves fed conventional concentrate feed in 90 days feeding trial. It was concluded that tree leaf meal (Leuceana leucocephala / Gliricidia sepium - 1:1) can be included up to 30% level in concentrate feed of goat kids and buffalo calves without any change in the growth rate but reducing feed cost