1,998 research outputs found
Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity
We have analyzed two residues in the helicase domain of the E1 initiator protein. These residues are part of a highly conserved structural motif, the beta-hairpin, which is present in the helicase domain of all papovavirus initiator proteins. These proteins are unique in their ability to transition from local template melting activity to unwinding. We demonstrate that the beta-hairpin has two functions. First, it is the tool used by the E1 double trimer (DT) to pry open and melt double-stranded DNA. Second, it is required for the unwinding activity of the hexameric E1 helicase. The fact that the same structural element, but not the same residues, contacts both dsDNA in the DT for melting and ssDNA in the double hexamer (DH) for helicase activity provides a link between local origin melting and DNA helicase activity and suggests how the transition between these two states comes about
Rotating mandrel speeds assembly of plastic inflatables
Rotating mandrel permits the accurate cutting, forming, and sealing of plastic gores for assembly of an inflatable surface of revolution. The gores remain on the mandrel until the final seam is reached. Tolerances are tightly controlled by the mandrel configuration
Deterministic Walks in Quenched Random Environments of Chaotic Maps
This paper concerns the propagation of particles through a quenched random
medium. In the one- and two-dimensional models considered, the local dynamics
is given by expanding circle maps and hyperbolic toral automorphisms,
respectively. The particle motion in both models is chaotic and found to
fluctuate about a linear drift. In the proper scaling limit, the cumulative
distribution function of the fluctuations converges to a Gaussian one with
system dependent variance while the density function shows no convergence to
any function. We have verified our analytical results using extreme precision
numerical computations.Comment: 18 pages, 9 figure
Dynamic look at DNA unwinding by a replicative helicase
A prerequisite for DNA replication is the unwinding of duplex DNA catalyzed by a replicative hexameric helicase. Despite a growing body of research, key elements of helicase mechanism remain under substantial debate. In particular, the number of DNA strands encircled by the helicase ring during unwinding and the ring orientation at the replication fork completely contrast in contemporary mechanistic models. Here we use single-molecule and ensemble assays to address these questions for the papillomavirus E1 helicase. We find that E1 unwinds DNA with a strand-exclusion mechanism, with the N-terminal side of the helicase ring facing the replication fork. We show that E1 generates strikingly heterogeneous unwinding patterns stemming from varying degrees of repetitive movements, which is modulated by the DNA-binding domain. Together, our studies reveal previously unrecognized dynamic facets of replicative helicase unwinding mechanisms
A strong pair correlation bound implies the CLT for Sinai Billiards
For Dynamical Systems, a strong bound on multiple correlations implies the
Central Limit Theorem (CLT) [ChMa]. In Chernov's paper [Ch2], such a bound is
derived for dynamically Holder continuous observables of dispersing Billiards.
Here we weaken the regularity assumption and subsequently show that the bound
on multiple correlations follows directly from the bound on pair correlations.
Thus, a strong bound on pair correlations alone implies the CLT, for a wider
class of observables. The result is extended to Anosov diffeomorphisms in any
dimension.Comment: 13 page
Peculiarities in produced particles emission in 208Pb + Ag(Br) interactions at 158 A GeV/c
The angular structures of particles produced in 208Pb induced collisions with
Ag(Br) nuclei in an emulsion detector at 158 A GeV/c have been investigated.
Nonstatistical ring-like substructures in azimuthal plane of the collision have
been found and their parameters have been determined. The indication on the
formation of the ring-like substructures from two symmetrical emission cones -
one in the forward and other in the backward direction in the center-of mass
system have been obtained. The ring-like substructures parameters have been
determined. The experimental results are in an agreement with I.M. Dremin idea,
that mechanism of the ring-like substructures formation in nuclear collisions
is similar to that of Cherenkov electromagnetic radiation.Comment: 10 pages, 7 figures, Report at the HADRON STRUCTURE'04 Conference,
Smolenice, Slovakia, 30.8.-3.9.200
- …