Method of treatment of viral hepatitis C in children with contraindications to standard antiviral therapy

The purpose of the work consists in obtaining the methods of treatment of chronic viral hepatitis C even in a situation when the patients have contraindications to the administration of standard antiviral therapy, and in improving the biochemical parameters and reducing the duration of hospitalization of the children with chronic viral hepatitis C. The presented materials demonstrate the effectiveness of an original method of treatment of viral hepatitis C in the case of high cytolysis associated with unbalanced diabetes, renal decompensation, non-viral autoimmune diseases, epilepsy and other diseases, which have contraindications to the administration of antiviral therapy. The basic therapy has been supplemented by the national phytopreparation Pacovirin in capsules with antiviral, immunomodulatory, antioxidant and interferonogenic activity. The efficacy of Pacovirin has been assessed on the basis of clinical, virological, immunological and biochemical indices. At the same time the analysis and evaluation of the results in the context of modern literature sources concerning the treatment of chronic hepatitis C in children with contraindications to standard antiviral therapy has been performed. The obtained results demonstrate an increased effectiveness of the treatment that is manifested by more rapid normalization of clinical and laboratory indices

Efficiency of the national drug pacovirin in the treatment of infectious mononucleosis in children

National Center of Public Health, the Republic of Moldova, Municipal Infectious Diseases Hospital for Children, Chisinau, Toma Ciorba Hospital of Infectious Diseases, Chisinau, the Republic of MoldovaBackground: In the article are presented the clinical results of the treatment of children with infectious mononucleosis where the basic therapy has been combined with the administration of the local drug pacovirin which has antiviral, immunomodulatory, interferonogenic and antioxidant activity. Material and methods: Pacovirin has been administered for one month by 50 mg capsules 2 times a day per os. The object of study has been presented by 175 patients (84 patients in the experimental group and 91 ones in the comparison group) and the records of the patients surveillance (form 003/e), containing the clinical diagnosis of infectious mononucleosis with Epstein-Barr virus and hepatitis syndrome. The duration of the treatment has been one month, the doses being age-appropriate and applied 2 times a day. Results: The administration of pacovirin as a medicinal remedy in addition to the traditional treatment, in accordance with the method proposed by us, has led to the more intensive normalization of clinical and laboratory indices, decrease of the pathological process and the rapid recovery of the patients, as well as the more intensive normalization of biochemical indices and significant reduction of the duration of the main clinical signs in the experimental group compared with the control group. Conclusions: The drug pacovirin has a positive effect on the main clinical signs of general toxic syndrome and digestive disorders, which have disappeared in a significant number of patients after the treatment. In most patients in the experimental group a decrease in the size of liver and spleen has been observed through palpatory evaluation by the end of the treatment course with pacovirin. In the the experimental group a positive dynamics of paraclinical indices (Alt, AST and γGTP) has been evident

Эпидемиологические и вирусологические аспекты гриппа, острых респираторных вирусных инфекций и тяжелых острых респираторных инфекций в Республике Молдова, сезон 2018/2019

Agenția Națională pentru Sănătate Publică, Congresul al VIII-lea al specialiștilor din domeniul sănătății publice și managementului sanitar 24-25 octombrie 2019 Chișinău, Republica MoldovaSezonul de gripă 2018/2019 a debutat mai devreme în comparație cu sezoanele precedente. Perioada epidemică a debutat în săptămâna 03/2019, atingând apogeul în săptămâna 06/2019 și s-a terminat în săptămâna 11/2019. Virusul gripal A(H1)pdm09 a fost predominant, fi ind detectat în 79,8% din cazurile de gripă confi rmate de laborator. Virusul gripal A(H3) a fost detectat în 20,2% cazuri. Virusurile gripale de tip B nu au fost detectate în acest sezon. Toate (4) virusurile gripale A(H1)pdm09 caracterizate genetic aparțin subgrupei 6B.1 (reprezentată prin A/Michigan/45/2015). Principalele caracteristici ale virusurilor din grupa 6B.1 sunt prezența substituțiilor aminoacizilor S84N, S162N (cu apariția unui nou loc potențial de glicozilare) și I216T în HA1. Virusurile gripale A/H3 detectate aparțin subclaselor 3C.2a1b și 3C.2a2. În ceea ce privește virusurile H3N2 circulante în prezent, caracterizarea lor antigenică prin reacția de hemaglutinoinhibare rămâne dificilă din cauza aglutinării instabile a globulelor roșii de cobai, curcan și umane. Nivelul morbidității prin gripă a sporit de 2,2 ori față de sezonul 2017/2018. Nivelul morbidității prin IACRS în sezonul 2018/2019 s-a majorat de 1,07 ori, iar prin SARI – de 1,17 ori față de sezonul precedent. Începând cu săptămâna 40/2018, virusurile respiratorii negripale au fost detectate în 37,7% (130/345) din probe colectate în timpul monitoringului în sistemul de tip santinelă. Au predominat rinovirusurile, adenovirusurile și virusul respirator sincițial.The influenza season started earlier that previous seasons. The epidemic period started at week 03/2019, peaked in week 06/2019 and ended in week 11/2019. Influenza A(H1)pdm09 was the predominant virus detected in 79,8% of ILI cases. Influenza A(H3) was detected in 20,2%. Influenza B viruses were not detected this season. All (4) of the A(H1)pdm09 viruses genetically characterized, belonged to the subgroup 6B.1 (represented by A/Michigan/45/2015). The main characteristics of viruses in the 6B.1 group are that the viruses carry the amino acid substitutions S84N, S162N (introducing a new potential glycosylation site) and I216T in HA1. Detected influenza A/ H3 viruses belonged to subclades 3C.2a1b and 3C.2a2. In the case of currently circulating H3N2 virus’s antigenic characterization continues to be difficult by HI assay due to variable agglutination of red blood cells from guinea pig, turkey and humans. The level of influenza morbidity increased 2,2 times compared to 2017/2018 season. The level of ARI morbidity in this season increased 1,07 times and the SARI recorded increased 1,17 times compared to the previous season. Since week 40/2018 other respiratory viruses were detected in 37,7% (130/345) of samples in sentinel surveillance. Rhinovirus, adenovirus and respiratory syncytial virus was detected in higher level during this season.Сезон гриппа 2018/2019 начался раньше, чем в предыдущие сезоны. Эпидемический период начался на неделе 03/2019, достигнув максимума на неделе 06/2019, и завершился на неделе 11/2019. Вирус гриппа A(H1)pdm09 был доминирующим и выявлен в 79,8% случаев гриппа. Вирус гриппа A(H3) был обнаружен в 20,2% случаев. Вирусы гриппа B не были выявлены в этом сезоне. Все (4) вирусы гриппа A(H1)pdm09б охарактеризованные генетически, относятся к подгруппе 6B.1 (представленную вирусом A/Michigan/45/2015). Основные характеристики вирусов из подгруппы 6B.1 – это наличие аминокислотных замен S84N, S162N (с появлением нового потенциального места гликозилирования) и I216T в HA1. Выявленные вирусы гриппа A/H3 относятся к подклассам 3C.2a1b и 3C.2a2. В случае циркулирующих в настоящее время H3N2, их антигенная характеристика с помощью реакции торможения гемаглютинации по-прежнему затруднена из за нестабильной агглютинации эритроцитов морской свинки, индейки и человека. Уровень заболеваемости гриппом повысился в 2,2 раза по сравнению с сезоном 2017/2018. Заболеваемость ОРВИ в сезоне 2018/2019 увеличилась в 1,07 раз по сравнению с предыдущим сезоном, а количество случаев ТОРИ увеличилось в 1,17 раз. Начиная с недели 40/2018 другие респираторные вирусы были выявлены в 37,7% (130/345) образцов, собранных во время дозорного надзора. Риновирусы, аденовирусы и респираторно-синцитиальные вирусы выявлялись в большом количестве в течении этого сезона

The effect of disorder on quantum phase transition in the double layered ruthenates (Sr1-xCax)3Ru2O7

(Sr1-xCax)3Ru2O7 is characterized by complex magnetic states, spanning from a long-range antiferromagnetically ordered state over an unusual heavy-mass nearly ferromagnetic (NFM) state to an itinerant metamagnetic (IMM) state. The NFM state, which occurs in the 0.4 > x > 0.08 composition range, freezes into a cluster-spin-glass (CSG) phase at low temperatures [Z. Qu et al., Phys. Rev. B 78, 180407(R) (2008)]. In this article, we present the scaling analyses of magnetization and the specific heat for (Sr1-xCax)3Ru2O7 in the 0.4 > x > 0.08 composition range. We find that in a temperature region immediately above the spin freezing temperature T$_f$, the isothermal magnetization M(H) and the temperature dependence of electronic specific heat C_e(T) exhibit anomalous power-law singularities; both quantities are controlled by a single exponent. The temperature dependence of magnetization M(T) also displays a power-law behavior, but its exponent differs remarkably from that derived from M(H) and C_e(T). Our analyses further reveal that the magnetization data M(H,T) obey a phenomenological scaling law of M(H,T) \propto H^\alpha f(H/T^\delta) in a temperature region between the spin freezing temperature T_f and the scaling temperature T_scaling. T_scaling systematically decreases with the decease of Ca content. This scaling law breaks down near the critical concentration x = 0.1 where a CSG-to-IMM phase transition occurs. We discussed these behaviors in term of the effect of disorder on the quantum phase transition.Comment: To be published in Phys. Rev.

The diversity and spread of genes encoding extended spectrum beta-lactamase enzymes in the strains of Escherichia coli

The aim of the study is to determine the mechanisms of antibiotic resistance of Escherichia coli (E. coli), the prevalence of extended spectrum betalactamase (ESBL) circulating strains of E. coli, establishing genotypes, phylogenetic groups of E. coli ESBL in the Republic of Moldova. On the basis of the data of local microbiological monitoring and using the phenotypic and molecular-genetic methods the genetic determinants (beta-lactamase), which cause the formation of resistance to beta-lactamase antibiotics have been identified. By polymerasis chaine reaction and the sequencing method the prevalence of E. coli strains producing ESBL in the urine probes has been identified. Such studies are unique for the Republic of Moldova and can serve as a basis for the establishment of the concept of causal and empirical treatment in our country. Urinary tract infections have been primarily (85%) determined by the E. coli species, followed by Enterococcus faecalis, Klebsiella pneumoniae, etc. The enzymes ESBL identified in the strains of E. coli in most of the cases have been of CTX-M type, the fact that explains the evolution and dissemination of E. coli producing ESBL. E. coli, a representative of the intestinal microflora, can serve as a reservoir of antibiotic resistance of microbic germs involved in the etiology of urinary tract infections. The detection of the type of beta-lactamase and the unification of the different subtypes of resistance to microorganisms are possible with the help of molecular biology techniques, by contrast with the phenotypic routine tests, the fact that is shown by the model of E. coli producing ESBL

Incommensurate magnetic order in the alpha-Fe(Te,Se) superconductor systems

Magnetic spin fluctuations is one candidate to produce the bosonic modes that mediate the superconductivity in the ferrous superconductors. Up until now, all of the LaOFeAs and BaFe2As2 structure types have simple commensurate magnetic ground states, as result of nesting Fermi surfaces. This type of spin-density-wave (SDW) magnetic order is known to be vulnerable to shifts in the Fermi surface when electronic densities are altered at the superconducting compositions. Superconductivity has more recently been discovered in alpha-Fe(Te,Se), whose electronically active antifluorite planes are isostructural to the FeAs layers found in the previous ferrous superconductors and share with them the same quasi-two-dimensional electronic structure. Here we report neutron scattering studies that reveal a unique complex incommensurate antiferromagnetic order in the parent compound alpha-FeTe. When the long-range magnetic order is suppressed by the isovalent substitution of Te with Se, short-range correlations survive in the superconducting phase.Comment: 27 pages, 7 figures, 1 tabl

A spin triplet supercurrent through the half-metallic ferromagnet CrO2

In general, conventional superconductivity should not occur in a ferromagnet, though it has been seen in iron under pressure. Moreover, theory predicts that the current is always carried by pairs of electrons in a spin singlet state, so conventional superconductivity decays very rapidly when in contact with a ferromagnet, which normally prohibits the existence of singlet pairs. It has been predicted that this rapid spatial decay would not occur when spin triplet superconductivity could be induced in the ferromagnet. Here we report a Josephson supercurrent through the strong ferromagnet CrO2, from which we infer that it is a spin triplet supercurrent. Our experimental setup is different from those envisaged in the earlier predictions, but we conclude that the underlying physical explanation for our result is a conversion from spin singlet to spin triplets at the interface. The supercurrent can be switched with the direction of the magnetization, analogous to spin valve transistors, and therefore could enable magnetization-controlled Josephson junctions.Comment: 14 pages, including 3 figure