Repository as a Service (RaaS)

In his oft-quoted seminal paper ‘Institutional Repositories: Essential Infrastructure For Scholarship In The Digital Age’ Clifford Lynch (2003) described the Institutional Repository as “a set of services that a university offers to the members of its community for the management and dissemination of digital materials created by the institution and its community members.” This paper seeks instead to define the repository service at a more primitive level, without the specialism of being an ‘Institutional Repository’, and looks at how it can viewed as providing a service within appropriate boundaries, and what that could mean for the future development of repositories, our expectations of what repositories should be, and how they could fit into the set of services required to deliver an Institutional Repository service as describe by Lynch

Limiter lock systems at TEXTOR: flexible tools for plasma-wall investigation

Limiter lock systems on the top and the bottom of the TEXTOR vessel are essential elements for experimental investigations of plasma-wall interaction in a tokamak. The lock systems are designed as user facilities that allow the insertion of wall elements (limiter) and tools for diagnostic (electrical probes, gas injection) without breaking the TEXTOR vacuum. The specially designed holder on top of the central carrier and a powerful vacuum pump system permit the exchange of components within similar to1 h. Up to ten electrical signals, four thermocouples, and a gas supply can be connected at the holder interface. Between discharges, the inserted component can be positioned radially and turned with respect to the toroidal magnetic field. Additionally, the central carrier is electrically isolated to apply bias voltages and currents up to 1 kV and 1 kA, respectively.An important feature of the lock system is the good access for optical spectroscopic observation of the inserted components in the vicinity of the edge plasma. The whole spectrum from ultraviolet to infrared is covered by spectrometers and filters combined with cameras. Toroidally and poloidally resolved measurements are obtained front the view on top of the probes while the tangential poloidal view delivers radially and toroidally resolved information.A programmable logic controller (Simatic S5) that is operated inside the TEXTOR bunker and from remote locations outside the concrete wall drives all possible features of the lock system

Osteoarthritis of the knee – clinical assessments and inflammatory markers11Supported by a grant from the Robert Bosch Stiftung, Stuttgart, Germany.

AbstractObjective: The present cross sectional study was performed to test the hypothesis that in osteoarthritis (OA) of the knee severity of this disease is related to local levels of inflammatory metabolites and their corresponding enzymes.Methods: From 41 patients with OA of the knee (age range 45–79 years) undergoing arthroscopy blood, synovial fluid (SF) and synovial membrane (SM) were collected. Clinical conditions were primarily assessed by the WOMAC-index and radiographic grading (K&L-grade). Concentrations of PGE2, TxB2and NO2/3and that of IL-6, IL-1α, IL-1β, TNFα, COX-2 and iNOS were determined in SF and SM, respectively.Results: With advancing age K&L-grade and COX-2 in SM increased significantly (P=0.005 and P=0.01, respectively). TNFα and IL-1α were not detectable in SM samples. Apart from a correlation between PGE2and WOMAC-index (r=0.36, P=0.035) no significant relationships could be found between the various inflammatory parameters and any of the assessed clinical signs.Conclusions: Apparently no direct relationships exist between the measured markers of inflammation (e.g. PGE2, NO2/3) or the involved enzymes (e.g. COX-2, iNOS) and the severity of OA of the knee. The degenerative condition of this disease might be due to the more local, mainly mechanical injury with little systemic upset. However, further longitudinal studies are needed to clarify whether the assessed biochemical markers could serve as predictors for the progression of OA

Microbial community responses determine how soil–atmosphere exchange of carbonyl sulfide, carbon monoxide, and nitric oxide responds to soil moisture

Carbonyl sulfide (OCS) plays an important role in the global sulfur cycle and is relevant for climate change due to its role as a greenhouse gas, in aerosol formation and atmospheric chemistry. The similarities of the carbon dioxide (CO2) and OCS molecules within chemical and plant metabolic pathways have led to the use of OCS as a proxy for global gross CO2 fixation by plants (gross primary production, GPP). However, unknowns such as the OCS exchange from soils, where simultaneous OCS production (POCS) and consumption (UOCS) occur, currently limits the use of OCS as a GPP proxy. We estimated POCS and UOCS by measuring net fluxes of OCS, carbon monoxide (CO), and nitric oxide (NO) in a dynamic chamber system fumigated with air containing different mixing ratios [OCS]. Nine soils with different land use were rewetted and soil–air exchange was monitored as soils dried out to assess responses to changing moisture. A major control of OCS exchange was the total amount of available sulfur in the soil. POCS production rates were highest for soils at WFPS (water-filled pore space) &gt;60&thinsp;% and rates were negatively related to thiosulfate concentrations. These moist soils switched from a net source to a net sink activity at moderate moisture levels (WFPS 15&thinsp;% to 37&thinsp;%). For three soils we measured NO and CO mixing ratios at different mixing ratios of OCS and revealed that NO and potentially CO exchange rates are linked to UOCS at moderate soil moisture. High nitrate concentrations correlated with maximum OCS release rates at high soil moisture. For one of the investigated soils, the moisture and OCS mixing ratio was correlated with different microbial activity (bacterial 16S rRNA, fungal ITS RNA relative abundance) and gene transcripts of red-like cbbL and amoA.</p

O ensino de PHP em páginas web dinâmicas através do desenvolvimento de um "fórum online sobre património cultural"

Relatório da Prática de Ensino Supervisionada, Mestrado em Ensino da Informática, Universidade de Lisboa, Instituto de Educação, 2020O presente relatório foi concretizado no âmbito da unidade curricular de Iniciação à Prática Profissional IV do Mestrado em Ensino da Informática do Instituto de Educação da Universidade de Lisboa. Retrata a intervenção pedagógica na Escola Secundária Gago Coutinho, numa turma do segundo ano do Curso Profissional de Técnico de Gestão e Programação de Sistemas Informáticos na disciplina de Redes de Comunicação e concretamente no final do módulo cinco: “Desenvolvimento de Páginas Web Dinâmicas” com a duração de catorze tempos letivos de quarenta e cinco minutos de prática de ensino supervisionada. Em virtude da suspensão das aulas presenciais por ordem do Governo a dezasseis de março de 2020 devido à pandemia COVID-19, quatro tempos decorreram presencialmente e dez tempos letivos decorreram à distância de forma síncrona via Zoom. Os conteúdos programáticos abordados foram os seguintes: sessões, cookies, tratamento de erros na linguagem PHP (PHP: Hypertext Preprocessor) e a consolidação de conhecimentos em linguagens PHP, HTML (Hyper Text Markup Language), CSS (Cascading Style Sheets) e SQL (Structured Query Language), que permitiram criar um fórum online sobre o património do concelho de Vila Franca de Xira que se enquadrou no tema do DAC (Domínio de Autonomia Curricular) da turma. Foi realizado um trabalho interdisciplinar e de articulação curricular privilegiando o trabalho prático e o desenvolvimento das capacidades de pesquisa, relação e análise, com vista ao desenvolvimento das áreas de competências inscritas no Perfil dos Alunos à Saída da Escolaridade Obrigatória. No decorrer da prática de ensino supervisionada foram usadas várias metodologias, nomeadamente a ativa, interrogativa e também a expositiva, de modo a minimizar as dificuldades identificadas por alguns autores no ensino dos princípios da programação aos alunos. Por fim, com o objetivo de avaliar de forma contínua as atitudes, o trabalho desenvolvido pelos alunos e a aquisição de conhecimentos, foram utilizadas ferramentas de avaliação diagnóstica, formativa e sumativa.The following report was accomplished in the curricular unit ambit of Initiation to Professional Practice IV Master’s Degree in Computer Science Teaching at Lisbon University, Educational Institute. The pedagogical intervention retracts the Secondary School Gago Coutinho, of an A-levels class from a Professional and Technical course in Management and Computer Systems Developer, considering the subject of communication networks, more precisely at the end of the 5th unit: “Dynamic Web Pages Development” the supervised teaching practice had the duration of 14 academic days which lasted 45 minutes each. Due to the COVID-19 pandemic, school suspension of presential classes was ordered by the government, March 16th of 2020, so only 4 academic days were attended. The remaining 10 occurred in long-distance learning synchronously using Zoom. The addressed programmatic contents were the following: sessions, cookies, language error treatment PHP (PHP: Hypertext Preprocessor) and knowledge in languages consolidation in PHP, HTML (HyperText Markup Language), CSS (Cascading Style Sheets) e SQL (Structured Query Language), which allowed to create an online forum about Vila Franca de Xira municipality Patrimony, and framed the theme CAD (Curricular Autonomy Dominance) of the class. An interdisciplinary work was carried out in the curricular articulation, privileging the practical work. Development of searching capabilities, relationship association, and analyse. Considering this, the intent is to develop the several knowledge areas enrolled in the student’s profile when they exit the obligatory schooling. During the practicing supervised taught, several methodologies were used. Such as active, interrogative, and expositive, to minimize the difficulties presented by some authors concerning teaching programming principal to students. To summarize, the objective is: To evaluate the attitudes continuously, the acquisition of knowledge, and work developed by the students. This became possible using tools of diagnostic, formative, and summative evaluation

The Cytotoxic Necrotizing Factor of Yersinia pseudotuberculosis (CNFy) is Carried on Extracellular Membrane Vesicles to Host Cells

In this study we show Yersinia pseudotuberculosis secretes membrane vesicles (MVs) that contain different proteins and virulence factors depending on the strain. Although MVs from Y. pseudotuberculosis YPIII and ATCC 29833 had many proteins in common (68.8% of all the proteins identified), those located in the outer membrane fraction differed significantly. For instance, the MVs from Y. pseudotuberculosis YPIII harbored numerous Yersinia outer proteins (Yops) while they were absent in the ATCC 29833 MVs. Another virulence factor found solely in the YPIII MVs was the cytotoxic necrotizing factor (CNFy), a toxin that leads to multinucleation of host cells. The ability of YPIII MVs to transport this toxin and its activity to host cells was verified using HeLa cells, which responded in a dose-dependent manner; nearly 70% of the culture was multinucleated after addition of 5 mu g/ml of the purified YPIII MVs. In contrast, less than 10% were multinucleated when the ATCC 29833 MVs were added. Semi-quantification of CNFy within the YPIII MVs found this toxin is present at concentrations of 5 -10 ng per mu g of total MV protein, a concentration that accounts for the cellular responses see