Immunoregulatory gene polymorphisms in women with preeclampsia

The costimulatory molecules CD28, cytotoxic T-lymphocyte antigen-4 (CTLA-4) (cytotoxic T-lymphocyte-associated antigen-4) and inducible costimulator (ICOS) are believed to have a critical modulatory role in the immune response. However, few studies have been performed on the role of these immune regulatory molecules and their polymorphisms in women with preeclampsia (PE). the aim of our study was to evaluate the CTLA4 (+49 A/G) (rs 231775), CD28 (+17 T/C) (rs 3116496) and ICOS (-1564 T/C) (rs 4675378) gene polymorphisms in Brazilian women with PE. This case-control study included 130 patients with PE and 261 control women without any obstetric or systemic disorders. Genomic DNA was extracted from peripheral blood, and the polymorphism genotyping was performed by digesting the PCR products with the restriction endonucleases BbvI (CTLA-4), Afel (CD28) and AluI (ICOS). Data were analyzed by X(2) or Fisher's exact test; a P-value of < 0.05 was considered as significant. There were significant differences in the ICOS genotype and allelic frequencies between the PE and control groups (P=0.01 and P=0.01, respectively). We found a significantly lower frequency of the ICOS (-1564) T allele in women with mild PE compared with the controls. There were no differences in the CTLA-4 (+49 A/G) and CD28 (+17 T/C) genotypes and allelic frequencies between the PE patients and controls. Our data suggest that PE is associated with ICOS, but is not associated with the CTLA-4 or CD28 gene polymorphisms. Hypertension Research (2011) 34, 384-388; doi:10.1038/hr.2010.247; published online 16 December 2010Fundacao de Amparo a PesquisaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de São Paulo, Dept Obstet, BR-01415002 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Obstet, BR-01415002 São Paulo, BrazilFundacao de Amparo a Pesquisa: 07/57446-0Web of Scienc

DomainSenticNet: An Ontology and a Methodology Enabling Domain-Aware Sentic Computing

In recent years, SenticNet and OntoSenticNet have represented important developments in the novel interdisciplinary field of research known as sentic computing, enabling the development of a variety of Sentic applications. In this paper, we propose an extension of the OntoSenticNet ontology, named DomainSenticNet, and contribute an unsupervised methodology to support the development of domain-aware Sentic applications. We developed an unsupervised methodology that, for each concept in OntoSenticNet, mines semantically related concepts from WordNet and Probase knowledge bases and computes domain distributional information from the entire collection of Kickstarter domain-specific crowdfunding campaigns. Subsequently, we applied DomainSenticNet to a prototype tool for Kickstarter campaign authoring and success prediction, demonstrating an improvement in the interpretability of sentiment intensities. DomainSenticNet is an extension of the OntoSenticNet ontology that integrates each of the 100,000 concepts included in OntoSenticNet with a set of semantically related concepts and domain distributional information. The defined unsupervised methodology is highly replicable and can be easily adapted to build similar domain-aware resources from different domain corpora and external knowledge bases. Used in combination with OntoSenticNet, DomainSenticNet may favor the development of novel hybrid aspect-based sentiment analysis systems and support further research on sentic computing in domain-aware applications