'Fat mass and obesity associated' gene (FTO): No significant association of variant rs9939609 with weight loss in a lifestyle intervention and lipid metabolism markers in German obese children and adolescents

<p>Abstract</p> <p>Background</p> <p>We have previously identified strong association of six single nucleotide polymorphisms (SNPs) in <it>FTO </it>(fat mass and obesity associated gene) to early onset extreme obesity within the first genome wide association study (GWA) for this phenotype. The aim of this study was to investigate whether the obesity risk allele of one of these SNPs (rs9939609) is associated with weight loss in a lifestyle intervention program. Additionally, we tested for association of rs9939609 alleles with fasting blood parameters indicative of glucose and lipid metabolism.</p> <p>Methods</p> <p>We initially analysed rs9939609 in a case-control study comprising 519 German overweight and obese children and adolescents and 178 normal weight adults. In 207 of the obese individuals who took part in the outpatient obesity intervention program 'Obeldicks' we further analysed whether carrier status of the obesity risk A-allele of rs9939609 has a differential influence on weight loss after the intervention program. Additionally, we investigated in 480 of the overweight and obese patients whether rs9939609 is associated with fasting blood levels of glucose, triglycerides and HDL and LDL-cholesterol. Genotyping was performed using allele specific polymerase chain reaction (ARMS-PCR). For the association study (case-control approach), the Cochran-Armitage trend test was applied. Blood parameters were analysed using commercially available test kits and the log10-transformed blood parameters and changes in BMI-standard deviation scores (BMI-SDS) were analysed by linear regression with sex and age as covariates under an additive mode of inheritance with the rs9939609 A-allele as risk allele.</p> <p>Results</p> <p>We confirmed the association of the risk A-allele of rs9939609 with overweight and early onset obesity (one sided p = 0.036). However, we observed no association of rs9939609 alleles with weight loss or fasting levels of blood glucose, triglycerides and cholesterol.</p> <p>Conclusion</p> <p>We confirmed the rs9939609 A-allele as a risk factor for early onset obesity whereas its impact on weight loss or on serum levels of glucose, triglycerides and cholesterol could not be detected in our samples.</p> <p>Trial Registration</p> <p><b>This study is registered </b>at clinicaltrials.gov (NCT00435734).</p

Long-term effect of lifestyle intervention on adiposity, metabolic parameters, inflammation and physical fitness in obese children: a randomized controlled trial

Epidemiology in Pediatrics and Child Healt

Características agronômicas dos acessos do Banco Ativo de Germoplasma de Uva.

Este trabalho teve por objetivo caracterizar agronomicamente 19 acessos do Banco Ativo de Germoplasma de Uva (BAGUva), por um período compreendido entre sete e 11 anos. Os materiais são cultivados a campo, em condições padronizadas, sob porta-enxerto '101-14?, com sistema de sustentação em espaldeira e poda em Guyot. Foram avaliadas características relacionadas ao cacho, à baga, à produtividade e à incidência das principais doenças da videira no país

Relative yield of wheat in coexistence with concurrent plants as indicator of competitiveness.

One of the factors that limit the yield of wheat is the weed competition, being the competitive potential of plants affected by their morphophysiological characteristics. The objective of this study was to determine the competitiveness of wheat when in coexistence with Italian ryegrass (Lolium multiflorum) and radish (Raphanus sativus) competitive plants or with wheat as simulator competition plant. There were realized four experiments in the greenhouse of the Agricultural Department of Environmental Sciences of the UFSM, Campus of Frederico Westphalen ? RS, during July to September, 2012. The treatments were arranged in a replacement series, in the proportions of 100:00 (wheat monoculture), 75:25, 50:50, 25:75 and 100:00 (competitor plant monoculture) for wheat plants (cv. Fundacep Cristalino) and for Italian ryegrass, radish and wheat (cv. BRS Guamirim) as competitor plants. The wheat is more competitive than Italian ryegrass in initial coexistence, even in the smallest proportion of plants. The radish when intercropped with wheat demonstrates similar competitive potential for environmental resources to the crop. There is mutual beneficial effect when wheat cultivars Fundacep Cristalino and BRS Guamirim coexisted, and each cultivar seems to have mechanism to avoid competition

Caracterização da qualidade do mosto de acessos do Banco Ativo de Germoplasma de Uva.

O objetivo deste trabalho foi avaliar a qualidade do mosto, com relação ao pH, conteúdo de sólidos solúveis totais (SS) e acidez total titulável (ATT) de 19 acessos do Banco Ativo de Germoplasma de Uva

Anatomy of the neural fibers at the superior mesenteric artery-a cadaver study

PURPOSE Most surgeons perform right-sided semicircular clearance of the superior mesenteric artery (SMA) nerve plexus for pancreatic head carcinoma, presuming a linear course of the SMA nerve fibers. The hypothesis was that the SMA nerve plexus fibers follow a non-linear course, and the goal of the present study was to assess the neural fibers distribution along the SMA. METHODS The course of neural fibers along the retropancreatic and suprapancreatic SMA was assessed in 7 cadavers. RESULTS In the retropancreatic course of the vessel, the main nerve cords branch and form a large number of finer nerve branches performing an anti-clockwise rotation of slightly less than 90° around the SMA. Finer nerve branches are located rather close to the vessel, while the main nerve cords are localized in the loose connective tissue of the peripheral parts of the vascular sheath. Nerve fibers around the suprapancreatic SMA run as two main nerve cords framing the artery on the right lateral-ventral and the left lateral to lateral-dorsal side. CONCLUSION The rotation of the nerve fiber around the SMA indicates that a more radical resection of at least 180° of neural tissue around the SMA might be required to achieve tumor clearance in pancreatic cancer with perineural invasion at the uncinate margin

Heisenberg model in a random field: phase diagram and tricritical behavior

By using the differential operator technique and the effective field theory scheme we study the tricritical behavior of Heisenberg classical model of spin-1/2 in a random field. The phase diagram in the T-h plane on a square and simple cubic lattice for a cluster with two spins is obtained when the random field is bimodal distributed.Comment: 10 pages, 1 figur

Análise fenológica de acessos mantidos pelo Banco Ativo de Germoplasma de Uva.

O objetivo deste trabalho foi avaliar o ciclo fenológico de 19 acessos mantidos no Banco Ativo de Germoplasma de Uva

Características agronômicas dos acessos do Banco Ativo de Germoplasma de Uva da Embrapa Uva e Vinho.

A caracterização de acessos de uva mantidos em coleção é uma etapa importante, que contribui para o melhoramento genético. Este trabalho teve como objetivo caracterizar agronomicamente 43 acessos do Banco Ativo de Germoplasma de Uva (BAG-Uva), mantido pela Embrapa Uva e Vinho, localizada em Bento Gonçalves-RS

Avaliação físico-química de acessos do Banco Ativo de Germoplasma de Uva (BAG-Uva).

O objetivo deste trabalho é caracterizar 43 acessos do BAG-Uva, com relação à qualidade do mosto [pH, Sólidos Solúveis Totais (SS) e Acidez Total Titulável (ATT)], por um período que variou entre sete e 10 anos. Trinta e oito acessos também foram avaliados com relação ao Índice de Polifenóis Totais (IPT) e conteúdo de antocianinas (ANT), por um período que variou de dois e três anos