Fe(III)-Chloroquine Complex: A New Potent Compound in Wellness Industries of High Antimicrobial and Antimalarial Activities

Fe(III)- Chloroquine complex has been synthesized and screened for its physicochemical, microbial as well as pharmacological activity have been done in solid and aqueous phase. On the basis of elemental analysis, polarographic studies, amperometric titration and IR spectral studies the probable formula for the complex has been determined at 25±1OC and ionic strength of µ= 1.0[KCl]. Raper's paper disc method was used for microbial study against various pathogenic bacteria and fungi. In vivo syudy of Swiss mice [25-30gm] were used for antimalarial activity against Chloroquine and its complex on xyline-Alcoholic activity test Kidney, liver and serum of these rats were also studied. On the basis of observed result it could be concluded that Fe (III)-Chloroquine complex were found to be non-toxic and more potent than pure chloroquine drug

Bio-Inorganic Studies on the Fe(II) Sparfloxacin Complex

The qualitative and quantitative analysis of an antibiotic drug, 5-amino-1 cyclopropyl-7 (cis-3, 5 dimethyl-1-piperazyl)-6,8- dihydro-1, 4 dihydro-4-oxo-3-quinoline carboxylic acid (Sparfloxacin, SFX) and its pharmaceutical formulation i.e.sparx-100 tablet, has been done using polarographic and amperometric methods. Complexation behavior of SFX with Fe(II), both in solid and liquid phases has been studied by elemental analysis, IR.-spectra and polarographic and amperometric methods. SFX produces a single cathodic reduction wave in 0.1 M ammonium tartrate (supporting electrolyte) at pH 6.0 ±0.1. The wave is diffusion controlled and wave height is proportional to the concentration of SFX. The complex is also reversibly reduced at the electrode surface with diffusion-controlled kinetics. The stoichiometry of the Fe(II)- SFX complex is 1:1. Antibacterial studies on the drug and its metal complex have been performed against different bacteria. The observed results revealed the complex to be more potent in its antibacterial activity as compared to the parent drug. On the basis of observed results it could be concluded that the prepared Fe(II)- SFX complex may be recommended to the therapeutic experts for its possible use as a more potent antibiotic drug

OnTheFly: a tool for automated document-based text annotation, data linking and network generation

OnTheFly is a web-based application that applies biological named entity recognition to enrich Microsoft Office, PDF and plain text documents. The input files are converted into the HTML format and then sent to the Reflect tagging server, which highlights biological entity names like genes, proteins and chemicals, and attaches to them JavaScript code to invoke a summary pop-up window. The window provides an overview of relevant information about the entity, such as a protein description, the domain composition, a link to the 3D structure and links to other relevant online resources. OnTheFly is also able to extract the bioentities mentioned in a set of files and to produce a graphical representation of the networks of the known and predicted associations of these entities by retrieving the information from the STITCH database

Short Co-occurring Polypeptide Regions Can Predict Global Protein Interaction Maps

A goal of the post-genomics era has been to elucidate a detailed global map of protein-protein interactions (PPIs) within a cell. Here, we show that the presence of co-occurring short polypeptide sequences between interacting protein partners appears to be conserved across different organisms. We present an algorithm to automatically generate PPI prediction method parameters for various organisms and illustrate that global PPIs can be predicted from previously reported PPIs within the same or a different organism using protein primary sequences. The PPI prediction code is further accelerated through the use of parallel multi-core programming, which improves its usability for large scale or proteome-wide PPI prediction. We predict and analyze hundreds of novel human PPIs, experimentally confirm protein functions and importantly predict the first genome-wide PPI maps for S. pombe (∼9,000 PPIs) and C. elegans (∼37,500 PPIs)

Global investigation of protein–protein interactions in yeast Saccharomyces cerevisiae using re-occurring short polypeptide sequences

Protein–protein interaction (PPI) maps provide insight into cellular biology and have received considerable attention in the post-genomic era. While large-scale experimental approaches have generated large collections of experimentally determined PPIs, technical limitations preclude certain PPIs from detection. Recently, we demonstrated that yeast PPIs can be computationally predicted using re-occurring short polypeptide sequences between known interacting protein pairs. However, the computational requirements and low specificity made this method unsuitable for large-scale investigations. Here, we report an improved approach, which exhibits a specificity of ∼99.95% and executes 16 000 times faster. Importantly, we report the first all-to-all sequence-based computational screen of PPIs in yeast, Saccharomyces cerevisiae in which we identify 29 589 high confidence interactions of ∼2 × 107 possible pairs. Of these, 14 438 PPIs have not been previously reported and may represent novel interactions. In particular, these results reveal a richer set of membrane protein interactions, not readily amenable to experimental investigations. From the novel PPIs, a novel putative protein complex comprised largely of membrane proteins was revealed. In addition, two novel gene functions were predicted and experimentally confirmed to affect the efficiency of non-homologous end-joining, providing further support for the usefulness of the identified PPIs in biological investigations

Losses in pendular suspensions due to centrifugal coupling

We present an analysis of the centrifugal coupling of a simple pendulum to a dissipative support. We show that such a coupling leads to an amplitude dependent quality factor. For amplitudes which could be present in laser interferometer gravitational wave detector suspensions, this mechanism could limit the quality factor of the test mass suspension significantly to 1010 and should be considered in the design of advanced LIGO type detectors

Crecimiento y caracterización de películas delgadas de V6 O13 por Sputtering Magnetron dc

Se reporta el crecimiento de películas delgadas de óxido de vanadio V6 O13 en fase pura sobre substratos amorfos, por la técnica de deposición sputtering reactivo con magnetrón dc. El estudio a partir de películas delgadas de este material ha tomado mucha importancia por sus propiedades electrocrómicas y su potencial uso como interruptores ópticos. Estudios de difracción de rayos x demostraron que las películas de V6O13 están altamente orientadas en la dirección (00l) . Las películas se lograron crecer bajo una atmósfera de oxígeno y argón con presión parcial de 0.8mTorr y 5.1mTorr respectivamente, y a una temperatura de substrato de 400ºC. Las películas fueron caracterizadas por sus propiedades ópticas a partir de medidas de transmitancia en el rango de luz visible y ultravioleta, así como por sus propiedades eléctricas midiéndose el cambio de resistencia en función de la temperatura. Reportamos el comportamiento que exhiben las películas de V6 O13 en radiación de largo de onda del IR. Las medidas de transmisión se hicieron en un rango de temperatura de 4 K a 180 K, rango en el cual el material experimenta cambio de fases de metálico. El estudio topográfico se hizo con microscopio fuerza atómica (AFM). A partir de los espectros de transmitancia en el rango de luz visible y ultravioleta se estimó en 2.75eV el ancho de banda prohibida del V6 O13, resultado que está dentro del rango de valores estimado para este material.Abstract: We report the growing of thin films of pure vanadium oxide V6 O13 on amorphous substrates, using the reactive sputtering technique with dc magnetron. The study of these thin films has become relevant due to their electrocromic properties and their potential to be used as optical switches. X rays diffraction studies showed that the thin films are highly oriented in the direction. They were grown under an oxygen and argon atmosphere and with a partial pressure of 0.8 mTorr and 5.1 mTorr respectively while the temperature was kept at 400 ºC. Optical properties of the thin films were studied measuring transmission in the visible light and ultraviolet ranges. Additional, electrical measurements of the resistance as a function of the temperature were obtained. We report the behavior that this films exhibit in the IR wavelength range. The transmission data was gathered in the temperature range of 4 to 180K, values within the material experiences a phase change. The topographic study was done with atomic force microscope (AFM). From the transmission spectra in the visible Light and ultraviolet range a energy gap of 2.75 eV was determined for the films, result that is in agreement with the known values for this material