An Unusual Melanocytic Nevus of Conjunctiva : Balloon Cell Nevus

Melanocytic nevi are one of the most common benign tumors of the conjunctiva. The balloon cell nevus is a rare and unusual variant of nevus of melanocytic origin, in which more than 50% of the tumor is composed of large polygonal cells with small nucleus and a clear to vacuolated cytoplasm. We describe a case of balloon cell nevus in a 6-year-old child who presented with pigmented lesion of the right eye since birth. The melanocytic nature of these cells was confirmed by immunohistochemical study. Excision of the tumor results in cure. This report describes a rare balloon cell nevus of conjunctiva which to the best of our knowledge is not documented in Indian literature

Cultural Sustainability: Sustaining Traditional Architecture for Tourism in Malaysia - a Case Study of Bangsar, Bukit Bintang and Sunway Lagoon Resort

The process of globalisation has both positive (technological advancement, bridging communities, breaking down cultural and political borders) and negative (eroding cultural values, creating standardised cities) impact on the lives of the society be it in the sphere of economy, politics, social or culture. The two most important facets of globalisation are the economy and culture. While the economic growth and development are manifested in terms of tourism development, the thrust of the tourism industry capitalises on the natural and cultural capital of a nation. A nation is greatly challenged to safeguard, preserve and conserve its cultural identity especially as it is also an asset in the tourism industry. The thrust of the thesis is based on the sustainable tourism development policy as advocated by WTO (1996) campaigning for equal power sharing relations between the local authority, private sector and the local community. The researcher relied on the triangulation method to obtain secondary and primary data. Interviews based on semi-structured and structured research schedules helped to obtain the qualitative and quantitative data from the government officers,local authority officials, private sector (tour and travel agents, hotel owners associations, and car rental associations) and the local community (local tourists, foreign tourists and non-governmental organisations). Data was processed and analysed using SPSS and content analysis methods. The findings indicate that the government plays a major role to shape the local traditional architectural landscape, especially at the study locations of Bangsar, Bukit Bintang and Sunway Lagoon Resort. While the private sector complemented with regular inputs and decorations at hotels, the local community did not play an active role in the planning or decision-making process. The locals were unhappy with the absence of local architectural designs at the study locations, but were satisfied with the presence of a well-managed and modern atmosphere. A suggestion for a Malaysian Sustainable Tourism Development Indicator was made incorporating the social and cultural elements

Report of Acoustic Test on PSLV IS.1/2L Structure

The results of acoustic conducted on PSLV IS.1/2L at Acoustic Test Facility are briefly given. It contains test set up, Instrumentation details and tables of spectral response

P R A methods for studying the impact of technology adoption in crop and poultry enterprises in a rural village

Participatory Rural Appraisal (PRA) methods have of late been highly successful in bridging the gap between development functionaries and target groups for whom the development straties and efforts have been focused at. PRA describes a growing family of approaches and methods to enable local people to share, enhance and analyze their knowledge of life and conditions, to plan and to act. (Chambers, 1994)

Livelihood analysis using wealth ranking tool of PRA

The present study was undertaken in Maroorpatti village in Namakkal district of Tamilnadu. From the results o/the livelihood analysis conducted/or the three different categories a/wealth ranking, it may be pointed out that with respect to the size of land holdings the rich farmer had double the size a/the medium and as such large as 10 times that the poor farmer. The rich farmer had an incomparably large number o/cattle with respect to his counterparts. While the rich farmer received monthly income from livestock, the medium farmer, who was thus classified solely by his agricultural land holdings, always expected his agricultural field to boost his monthly income. Furthermore, from the expenditure pal1ern observed, the rich farmer's were inclined to save nearly 35% of their monthly income, while the poor farmers were always in need of 25% loan to meet their monthly needs. As a consequence, the crises analysis indicated that the poor farmer as well as the medium farmer usually takes a loan of 40% towards meeting any calamities faced in their agricultural and livestock sectors

Application of PRA technique for field problem identification

This paper deals with the application of participatory rural appraisal technique for identification of agricultural field problems of Maroorpatti village . The economic importance of problems was also worked out

Biophysics at the coffee shop: lessons learned working with George Oster

Over the past 50 years, the use of mathematical models, derived from physical reasoning, to describe molecular and cellular systems has evolved from an art of the few to a cornerstone of biological inquiry. George Oster stood out as a pioneer of this paradigm shift from descriptive to quantitative biology not only through his numerous research accomplishments, but also through the many students and postdocs he mentored over his long career. Those of us fortunate enough to have worked with George agree that his sharp intellect, physical intuition and passion for scientific inquiry not only inspired us as scientists but also greatly influenced the way we conduct research. We would like to share a few important lessons we learned from George in honor of his memory and with the hope that they may inspire future generations of scientists.Comment: 22 pages, 3 figures, accepted in Molecular Biology of the Cel

Highly efficient transfection of human induced pluripotent stem cells using magnetic nanoparticles.

PurposeThe delivery of transgenes into human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) represents an important tool in cardiac regeneration with potential for clinical applications. Gene transfection is more difficult, however, for hiPSCs and hiPSC-CMs than for somatic cells. Despite improvements in transfection and transduction, the efficiency, cytotoxicity, safety, and cost of these methods remain unsatisfactory. The objective of this study is to examine gene transfection in hiPSCs and hiPSC-CMs using magnetic nanoparticles (NPs).MethodsMagnetic NPs are unique transfection reagents that form complexes with nucleic acids by ionic interaction. The particles, loaded with nucleic acids, can be guided by a magnetic field to allow their concentration onto the surface of the cell membrane. Subsequent uptake of the loaded particles by the cells allows for high efficiency transfection of the cells with nucleic acids. We developed a new method using magnetic NPs to transfect hiPSCs and hiPSC-CMs. HiPSCs and hiPSC-CMs were cultured and analyzed using confocal microscopy, flow cytometry, and patch clamp recordings to quantify the transfection efficiency and cellular function.ResultsWe compared the transfection efficiency of hiPSCs with that of human embryonic kidney (HEK 293) cells. We observed that the average efficiency in hiPSCs was 43%±2% compared to 62%±4% in HEK 293 cells. Further analysis of the transfected hiPSCs showed that the differentiation of hiPSCs to hiPSC-CMs was not altered by NPs. Finally, robust transfection of hiPSC-CMs with an efficiency of 18%±2% was obtained.ConclusionThe difficult-to-transfect hiPSCs and hiPSC-CMs were efficiently transfected using magnetic NPs. Our study offers a novel approach for transfection of hiPSCs and hiPSC-CMs without the need for viral vector generation