32 research outputs found
Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of beta-catenin in adrenocortical carcinoma
Adrenocortical cancer (ACC) is a very aggressive tumor, and genomics studies demonstrate that the most frequent alterations of driver genes in these cancers activate the Wnt/beta-catenin signaling pathway. However, the adrenal-specific targets of oncogenic beta-catenin-mediating tumorigenesis have not being established. A combined transcriptomic analysis from two series of human tumors and the human ACC cell line H295R harboring a spontaneous beta-catenin activating mutation was done to identify the Wnt/beta-catenin targets. Seven genes were consistently identified in the three studies. Among these genes, we found that AFF3 mediates the oncogenic effects of beta-catenin in ACC. The Wnt response element site located at nucleotide position - 1408 of the AFF3 transcriptional start sites (TSS) mediates the regulation by the Wnt/beta-catenin signaling pathway. AFF3 silencing decreases cell proliferation and increases apoptosis in the ACC cell line H295R. AFF3 is located in nuclear speckles, which play an important role in RNA splicing. AFF3 overexpression in adrenocortical cells interferes with the organization and/or biogenesis of these nuclear speckles and alters the distribution of CDK9 and cyclin T1 such that they accumulate at the sites of AFF3/speckles. We demonstrate that AFF3 is a new target of Wnt/beta-catenin pathway involved in ACC, acting on transcription and RNA splicing
Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of ÎČ-catenin in adrenocortical carcinoma
International audienceAdrenocortical cancer (ACC) is a very aggressive tumor, and genomics studies demonstrate that the most frequent alterations of driver genes in these cancers activate the Wnt/ÎČ-catenin signaling pathway. However, the adrenal-specific targets of oncogenic ÎČ-catenin-mediating tumorigenesis have not being established. A combined transcriptomic analysis from two series of human tumors and the human ACC cell line H295R harboring a spontaneous ÎČ-catenin activating mutation was done to identify the Wnt/ÎČ-catenin targets. Seven genes were consistently identified in the three studies. Among these genes, we found that AFF3 mediates the oncogenic effects of ÎČ-catenin in ACC. The Wnt response element site located at nucleotide position â 1408 of the AFF3 transcriptional start sites (TSS) mediates the regulation by the Wnt/ÎČ-catenin signaling pathway. AFF3 silencing decreases cell proliferation and increases apoptosis in the ACC cell line H295R. AFF3 is located in nuclear speckles, which play an important role in RNA splicing. AFF3 overexpression in adrenocortical cells interferes with the organization and/or biogenesis of these nuclear speckles and alters the distribution of CDK9 and cyclin T1 such that they accumulate at the sites of AFF3/speckles. We demonstrate that AFF3 is a new target of Wnt/ÎČ-catenin pathway involved in ACC, acting on transcription and RNA splicing
Combined transcriptome studies identify AFF3 as a mediator of the oncogenic effects of beta-catenin in adrenocortical carcinoma
Adrenocortical cancer (ACC) is a very aggressive tumor, and genomics studies demonstrate that the most frequent alterations of driver genes in these cancers activate the Wnt/beta-catenin signaling pathway. However, the adrenal-specific targets of oncogenic beta-catenin-mediating tumorigenesis have not being established. A combined transcriptomic analysis from two series of human tumors and the human ACC cell line H295R harboring a spontaneous beta-catenin activating mutation was done to identify the Wnt/beta-catenin targets. Seven genes were consistently identified in the three studies. Among these genes, we found that AFF3 mediates the oncogenic effects of beta-catenin in ACC. The Wnt response element site located at nucleotide position - 1408 of the AFF3 transcriptional start sites (TSS) mediates the regulation by the Wnt/beta-catenin signaling pathway. AFF3 silencing decreases cell proliferation and increases apoptosis in the ACC cell line H295R. AFF3 is located in nuclear speckles, which play an important role in RNA splicing. AFF3 overexpression in adrenocortical cells interferes with the organization and/or biogenesis of these nuclear speckles and alters the distribution of CDK9 and cyclin T1 such that they accumulate at the sites of AFF3/speckles. We demonstrate that AFF3 is a new target of Wnt/beta-catenin pathway involved in ACC, acting on transcription and RNA splicing
Leaching of copper-refined anode slime
EThOS - Electronic Theses Online ServiceGBUnited Kingdo
Etude de lâauto-adaptivitĂ© du filtre actif parallĂšle aux variations de la charge
Cet article sâintĂ©resse Ă lâamĂ©lioration des performances du filtre actif parallĂšle pour sâadapter dâune maniĂšre automatique aux variations de la charge. Ce filtre est un onduleur de tension Ă MLI destinĂ© Ă Ă©liminer les harmoniques de courant gĂ©nĂ©rĂ©s par un pont redresseur triphasĂ© non commandĂ© (charge non linĂ©aire). Pour assurer lâauto-additivitĂ© du filtre aux variations de la charge, deux schĂ©mas de rĂ©gulation Ă base du rĂ©gulateur P sont proposĂ©s, lâun pour la tension continue et lâautre pour le courant injectĂ©. Les rĂ©sultats obtenus par simulation ont montrĂ©s une amĂ©lioration des performances de ce filtre. Le taux de distorsion harmonique (THD) calculĂ© aprĂšs filtrage est infĂ©rieur Ă 5% et le filtre sâadapte parfaitement aux variations de la charge. Mots clĂ©s: filtre actif parallĂšle; harmoniques; onduleur Ă MLI; taux de distorsion dâharmonique; mĂ©thode p-q. The purpose of this work is to improve shunt active filter performances to cope automatically with load changes. This filter is a PWM inverter used to reduce harmonic currents generated by uncontrolled three phase bridge rectifier (non linear load). To ensure filter auto-adaptivity, two control schemes based on P controller are proposed; one for dc voltage control and the second for the injected current (active filter current) control. The obtained results by simulation have showed effectiveness and improvement in the performances of the filter. The calculated total harmonic distortion (THD) factor is less than 5% and the filter copes with load variations.Keywords: shunt active filter; harmonics; PWM inverter; total harmonic distortion; p-q theory