Detection and quantification of Lyme spirochetes using sensitive and specific molecular beacon probes

<p>Abstract</p> <p>Background</p> <p>Lyme disease, caused by <it>Borrelia burgdorferi</it>, affects a large number of people in both the USA and Europe. The mouse is a natural host for this spirochete and is widely used as a model system to study Lyme pathogenesis mechanisms. Since disease manifestations often depend upon the spirochete burden in a particular tissue, it is critical to accurately measure the bacterial number in infected tissues. The current methods either lack sensitivity and specificity (SYBR Green), or require independent analysis of samples in parallel to quantitate host and bacterial DNA (TaqMan). We have developed a novel molecular beacon-based convenient multiplex real-time quantitative PCR assay to identify and detect small numbers of <it>B. burgdorferi </it>in infected mouse tissues.</p> <p>Results</p> <p>We show here that molecular beacons are effective, sensitive and specific probes for detecting and estimating wide-ranging numbers of <it>B. burgdorferi </it>in the presence of mouse DNA. In our assays, the spirochete <it>recA </it>and the mouse <it>nidogen </it>gene amplicons were detected simultaneously using molecular beacons labeled with different fluorophores. We further validated the application of these probes by quantifying the wild-type strain and <it>bgp</it>-defective mutant of <it>B. burgdorferi</it>. The <it>bgp</it>-defective mutant shows a ten-fold reduction in the level of spirochetes present in various tissues.</p> <p>Conclusion</p> <p>The high sensitivity and specificity of molecular beacons makes them superior probes for the detection of small numbers of <it>B. burgdorferi</it>. Furthermore, the use of molecular beacons can be expanded for the simultaneous detection and quantification of multiple pathogens in the infected hosts, including humans, and in the arthropod vectors.</p

Processing of ilmenite (FeOTiO2) for value added products

Beach Placer Ilmenite is an important source for production of titanium metal, titania slag and pigment grade titanium dioxide. Besides, these well known applications for which Ilmenite is mined and processed, there are other emerging processes that are being tried for obtaining highvalue products. Synthesis of Ilmenite based materials for varistor applications and direct electrochemical reduction of Ilmenite to produce ferrotitanium are two such attempts being discussed in this work. In this paper, recent efforts undertaken to study the electrical and magnetic characteristics are discussed. Ilmenite, FeTiO 3 , is one of the mixed-valence transition metalbearing minerals, inwhich Fe can be in two different oxidation states, Fe 2+ and Fe 3+ .. Similarly, Ti can be in Ti 3+ and Ti 4+ .Ilmenite is inherently suitable for making Varistors, which are devices, used for limiting the transient voltage surges in a circuit. Varistors produced from Ilmenite can withstand harsh environments seen in nuclear reactors and outerspace. Electrochemical reduction of Ilmenite can be used to prepare ferrotitanium directly without any reductant. The process essentially involves removal of oxygen from the mineral through electrolytic action using calcium chloride as electrolyte and graphite as anode. At a temperature of 950°C, it is shown to be possible that all the oxygen can be removed from the Ilmenite sample, which passes through the electrolyte, to form CO/CO 2 at the anode. The process has immense potential for cost effective production of titanium metal as well. Results of the above developmental works are presented in this paper

A Clinical study on the efficacy of Udvartana and Nasya on Ovulatory functions w.s.r. to Female infertility in Moderate Obese (Sthula) individuals

Childlessness and infertility are rising dramatically in cities. The increase may be due to many reasons like the way of living, coping with stress, job pressure, obesity, postponing parenthood, galloping urbanization, sexually transmitted infections and many others. Hence treating infertility is the challenging job for the doctors in present era. The failure to ovulate is the major problem in approximately 40% cases of female infertility. This can be anovulation or severe oligo ovulation. In the latter case even though the ovulation does occur, its relative infrequency decreases the woman's chance of pregnancy. Hence the infertility and especially ovarian factor induced female infertility needs an immediate attention from alternative medicines. Over last decades, fertility therapy has expanded more than any other field of medicine. Hormonal therapy, In vitro Fertilization (IVF), Embryo Transfer (ET), Gamete Intra Fallopian Transfer (GIFT) etc., all these have a minimal success rate. Additionally these procedures are associated with adverse effects &amp; are not affordable to all. Ayurveda may give a promising hand to cure this condition with the various treatment modalities mentioned in the classics. For the present clinical study, an effort was made to study the efficacy of Udvartana and Nasya followed by Shamanaushadhis in reversing the pathology of this disease and giving a “Never-Ending Joy” of parenthood to the couples through the Ayurvedic treatment

Rakshak - A Rescue Bot

Robotics is the field of designing and innovation that assumes a vital part in making our lives simpler to perform different undertakings. The primary point of the venture is to outline and build up, a sensor based mechanical arm which is controlled by human hand. The venture manages controlling a servo mechanical arm with the assistance of movement detecting innovation by Flex sensor and 3-hub accelerometer. The pick and place operation of the mechanical arm can be controlled utilizing Arduino. This framework can be utilized as a part of enterprises, dangerous conditions, biomedical surgeries and different applications

Serotype-specific differences in inhibition of reovirus infectivity by human-milk glycans are determined by viral attachment protein σ1

AbstractHuman milk contains many bioactive components, including secretory IgA, oligosaccharides, and milk-associated proteins. We assessed the antiviral effects of several components of milk against mammalian reoviruses. We found that glucocerebroside (GCB) inhibited the infectivity of reovirus strain type 1 Lang (T1L), whereas gangliosides GD3 and GM3 and 3′-sialyllactose (3SL) inhibited the infectivity of reovirus strain type 3 Dearing (T3D). Agglutination of erythrocytes mediated by T1L and T3D was inhibited by GD3, GM3, and bovine lactoferrin. Additionally, α-sialic acid, 3SL, 6′-sialyllactose, sialic acid, human lactoferrin, osteopontin, and α-lactalbumin inhibited hemagglutination mediated by T3D. Using single-gene reassortant viruses, we found that serotype-specific differences segregate with the gene encoding the viral attachment protein. Furthermore, GD3, GM3, and 3SL inhibit T3D infectivity by blocking binding to host cells, whereas GCB inhibits T1L infectivity post-attachment. These results enhance an understanding of reovirus cell attachment and define a mechanism for the antimicrobial activity of human milk

Adult immersion test for assessment of amitraz resistance in Rhipicephalus sanguineus ticks

Ticks are obligate blood sucking ectoparasites recognised globally as vectors of various pathogens, next to mosquitoes. The present study is conducted on brown dog ticks collected from naturally infested dogs in and around Thrissur district. Adult immersion test was performed and the parameters such as engorged tick weight, egg mass weight as well as tick mortality were recorded to derive the reproductive indices, percentage inhibition of oviposition and percentage mortalities. Non-linear regression analysis of the dose response data yielded the LC50 values at 95% confidence intervals and, the slopes and regression coefficients of the different parameters were also derived. The Resistance factors (RF) were calculated to categorise resistance levels into I, II, III and IV. Resistance to amitraz was detected at level II in tick isolates collected from Choolissery and Mundur, at level III and level IV in isolates collected from Mannuthy and Paravattani, respectively. Two isolates collected from Mannuthy were found to be susceptible. One way ANOVA of resistance levels revealed that the resistance levels III and IV were significantly different from that of susceptible and resistance level II. This is the first report on detection of amitraz resistance levels in dog ticks from south India calling for proper implementation of tick control strategies to prevent spread of amitraz resistance

Lactation and neonatal nutrition: defining and refining the critical questions.

This paper resulted from a conference entitled "Lactation and Milk: Defining and refining the critical questions" held at the University of Colorado School of Medicine from January 18-20, 2012. The mission of the conference was to identify unresolved questions and set future goals for research into human milk composition, mammary development and lactation. We first outline the unanswered questions regarding the composition of human milk (Section I) and the mechanisms by which milk components affect neonatal development, growth and health and recommend models for future research. Emerging questions about how milk components affect cognitive development and behavioral phenotype of the offspring are presented in Section II. In Section III we outline the important unanswered questions about regulation of mammary gland development, the heritability of defects, the effects of maternal nutrition, disease, metabolic status, and therapeutic drugs upon the subsequent lactation. Questions surrounding breastfeeding practice are also highlighted. In Section IV we describe the specific nutritional challenges faced by three different populations, namely preterm infants, infants born to obese mothers who may or may not have gestational diabetes, and infants born to undernourished mothers. The recognition that multidisciplinary training is critical to advancing the field led us to formulate specific training recommendations in Section V. Our recommendations for research emphasis are summarized in Section VI. In sum, we present a roadmap for multidisciplinary research into all aspects of human lactation, milk and its role in infant nutrition for the next decade and beyond

Conflict, Crisis, and Abuse in Dharavi, Mumbai: Experiences from Six Years at a Centre for Vulnerable Women and Children

Nayreen Daruwalla and colleagues describe the Centre for Vulnerable Women and Children, which serves clients coping with crisis and violence in the urban setting of Dharavi, Mumbai

Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymenas germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.</p

Comparative Genomics of Emerging Human Ehrlichiosis Agents

Anaplasma (formerly Ehrlichia) phagocytophilum, Ehrlichia chaffeensis, and Neorickettsia (formerly Ehrlichia) sennetsu are intracellular vector-borne pathogens that cause human ehrlichiosis, an emerging infectious disease. We present the complete genome sequences of these organisms along with comparisons to other organisms in the Rickettsiales order. Ehrlichia spp. and Anaplasma spp. display a unique large expansion of immunodominant outer membrane proteins facilitating antigenic variation. All Rickettsiales have a diminished ability to synthesize amino acids compared to their closest free-living relatives. Unlike members of the Rickettsiaceae family, these pathogenic Anaplasmataceae are capable of making all major vitamins, cofactors, and nucleotides, which could confer a beneficial role in the invertebrate vector or the vertebrate host. Further analysis identified proteins potentially involved in vacuole confinement of the Anaplasmataceae, a life cycle involving a hematophagous vector, vertebrate pathogenesis, human pathogenesis, and lack of transovarial transmission. These discoveries provide significant insights into the biology of these obligate intracellular pathogens