38 research outputs found
Circular Dichroism Studies on C-terminal Zinc Finger Domain of Transcription Factor GATA-2
The C-terminal zinc finger domain of GATA-2 transcription factor from Rattus norvegicus has been expressed and purified to elucidate its secondary structure using circular dichroism spectroscopy. Circular dichroism spectra showed that native GATA-2 C-terminal domain of (Cys)4 type zinc finger has 12% α-helix, 36% β-sheet and 52% random coil content. The estimated structure was compared with predicted structures by sequence based prediction software SOPMA and found to be similar. Furthermore, the effect of pH on the secondary structure of GATA-2 C-finger was examined. This study provides an insight into the understanding of the structure and function of transcription factor GATA-2
Mechanism of detoxification of aluminum ions by kraft lignin treated with alkaline oxygen
Spectral analysis of the successive degradation process of kraft lignin by alkaline oxygen treatment
On the mechanism of the formation of formaldehyde in the radiolysis of deaerated methanol
Analysis of progress of oxidation reaction during oxygen-alkali treatment of lignin I: method and its application to lignin oxidation
High-resolution nuclear magnetic resonance spectroscopic study of metabolites in the cerebrospinal fluid of patients with cervical myelopathy and lumbar radiculopathy
Ethanol contamination of cerebrospinal fluid during standardized sampling and its effect on 1H-NMR metabolomics
Standardization of body fluid sampling, processing and storage procedures is pivotal to ensure data quality in metabolomics studies. Yet, despite strict adherence to standard sampling guidelines, we detected variable levels of ethanol in the (1)H-NMR spectra of human cerebrospinal fluid (CSF) samples (range 9.2 × 10(−3)–10.0 mM). The presence of ethanol in all samples and the wide range of concentrations clearly indicated contamination of the samples of some sort, which affected the (1)H-NMR spectra quality and the interpretation. To determine where in the sampling protocol the ethanol contamination occurs, we performed a CSF sampling protocol simulation with 0.9 % NaCl (saline) instead of CSF and detected ethanol in all simulation samples. Ethanol diffusion through air during sampling and preparation stages appeared the only logical explanation. With a bench study, we showed that ethanol easily diffuses into ex vivo CSF samples via air transmission. Ethanol originated from routinely used skin disinfectants containing ethanol and from laboratory procedures. Ethanol affected the CSF sample matrix at concentrations above ~9.4 mM and obscured a significant part of the (1)H-NMR spectrum. CSF sample preparation for (1)H-NMR-based metabolomics analyses should therefore be carried out in a well-ventilated atmosphere with laminar flow, and use of ethanol should be avoided. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00216-015-8663-9) contains supplementary material, which is available to authorized users