A Solvent-free Synthesis of Polyhydroquinolines via Hantzsch Multicomponent Condensation Catalyzed by Nanomagnetic-supported Sulfonic Acid

A simple and efficient procedure for the synthesis of polyhydroquinolines was developed, involving a one-pot four-component Hantzsch condensation of aromatic aldehydes, 1,3-cyclohexanediones, alkyl acetoacetate and ammonium acetate in the presence of a catalytic amount of nanomagnetic-supported sulfonic acid under solvent-free conditions. The method offers several advantages including high yields, short reaction times, a simple work-up procedure and catalyst reusability for several runs. Furthermore, easy isolation of the catalyst from the reaction mixture was enabled by use of an external magnet.KEYWORDS Nanomagnetic-supported sulfonic acid, multicomponent reaction, solvent-free conditions, heterocyclic compound, Hantzsch reaction

Preparation of palladated porous nitrogen-doped carbon using halloysite as porogen: disclosing its utility as a hydrogenation catalyst

In this article, halloysite nanoclay (Hal) was used as porogen for the synthesis of nitrogen doped porous carbon material with high specific surface area and pore volume. To this purpose, polymerization of melamine and terephthalaldehyde (MT) was performed in the presence of amine-functionalized carbon coated Hal (Hal@Glu-2N) that was prepared from hydrothermal treatment of Hal and glucose. Then, the prepared nanocomposite was palladated and carbonized to afford Pd@Hal@C. To further improve the textural properties of the nanocomposite, and introduce more pores in its structure, Hal nanotubes were etched. The characterization of the resulting compound, Pd@C, and comparing it with Pd@Hal@C, showed that etching of Hal significantly increased the specific surface area and pore volume in Pd@C. Pd@C was successfully used as a heterogeneous catalyst for promoting hydrogenation of nitroarens in aqueous media using hydrogen with atmospheric pressure as a reducing agent. The comparison of the structural features and catalytic activity of the catalyst with some control catalysts, including, Pd@Hal, Pd@Hal@Glu, Pd@Hal@Glu-MT and Pd@Hal@C confirmed that nitrogen groups in C could improve the Pd anchoring and suppress its leaching, while etching of Hal and introduction of more pores could enhance the catalytic activity through facilitating the mass transfer

Cloning, expression, purification and antigenicity of antigenic region of MOMP protein from chlamydia trachomatis

Introduction: Chlamydia trachomatis is an obligate intracellular parasites, gram-negative bacteria and also is one of the most common sexually transmitted infections. Correspondingly, the MOMP protein contains more than 60 of the extra-membrane proteins, which indicates the importance of this protein for the detection of Chlamydia trachomatis. The purpose of the study is to produce recombinant protein composed of antigenic regions of the MOMP protein and its antigenicity. Materials and Methods: In this study, the regions with the highest antigenic property of the omp1 gene, with a length of 674 bp, were obtained based on bioinformatics software's and linked with flexible linker. Then, it was cloned and expressed in plasmid vector pET32a. After purification of the recombinant protein, its antigenicity was evaluated using Western blot technique with serum of infected people with Chlamydia. Results: Based on the results, in all serum samples of patients with chlamydial infections, antibody response bands were observed in nitrocellulose paper. Anyway, no antibody response band was detected in the serum samples of healthy people. Conclusion: Data showed that antigenic region of MOMP protein can be expressed by in E. coli. This protein was recognized by sera patients suffering from Chlamydia trachomatis infection. Conclusively, the recombinant protein has similar epitopes and close antigenic properties to the natural form of this antigen. © 2017, Semnan University of Medical Sciences. All rights reserved

Cloning, expression, purification and antigenicity of antigenic region of MOMP protein from chlamydia trachomatis

Introduction: Chlamydia trachomatis is an obligate intracellular parasites, gram-negative bacteria and also is one of the most common sexually transmitted infections. Correspondingly, the MOMP protein contains more than 60 of the extra-membrane proteins, which indicates the importance of this protein for the detection of Chlamydia trachomatis. The purpose of the study is to produce recombinant protein composed of antigenic regions of the MOMP protein and its antigenicity. Materials and Methods: In this study, the regions with the highest antigenic property of the omp1 gene, with a length of 674 bp, were obtained based on bioinformatics software's and linked with flexible linker. Then, it was cloned and expressed in plasmid vector pET32a. After purification of the recombinant protein, its antigenicity was evaluated using Western blot technique with serum of infected people with Chlamydia. Results: Based on the results, in all serum samples of patients with chlamydial infections, antibody response bands were observed in nitrocellulose paper. Anyway, no antibody response band was detected in the serum samples of healthy people. Conclusion: Data showed that antigenic region of MOMP protein can be expressed by in E. coli. This protein was recognized by sera patients suffering from Chlamydia trachomatis infection. Conclusively, the recombinant protein has similar epitopes and close antigenic properties to the natural form of this antigen. © 2017, Semnan University of Medical Sciences. All rights reserved

CMSRPC efficiency measurement using the tag-and-probe method

We measure the efficiency of CMS Resistive Plate Chamber (RPC) detectors in proton-proton collisions at the centre-of-mass energy of 13 TeV using the tag-and-probe method. A muon from a Z(0) boson decay is selected as a probe of efficiency measurement, reconstructed using the CMS inner tracker and the rest of CMS muon systems. The overall efficiency of CMS RPC chambers during the 2016-2017 collision runs is measured to be more than 96% for the nominal RPC chambers

RPC radiation background simulations for the high luminosity phase in the CMS experiment

The high luminosity expected from the HL-LHC will be a challenge for the CMS detector. The increased rate of particles coming from the collisions and the radioactivity induced in the detector material could cause significant damage and result in a progressive degradation of its performance. Simulation studies are very useful in these scenarios as they allow one to study the radiation environment and the impact on detector performance. Results are presented for CMS RPC stations considering the operating conditions expected at the HL-LHC