МЕТОДИЧНІ ВКАЗІВКИ та завдання до практичних занять і самостійної роботи З НАВЧАЛЬНОЇ ДИСЦИПЛІНИ «НАРИСНА ГЕОМЕТРІЯ». ЧАСТИНА 4. ПЕРСПЕКТИВА для здобувачів вищої освіти першого (бакалаврського) рівня за спеціальністю 191 «Архітектура та містобудування» денної форми навчання. METHODICAL GUIDELINES and tasks to a practical training and independent work IN THE DISCIPLINE «DESCRIPTIVE GEOMETRY». PART 4. PROSPECT for higher education students of the first (bachelor) level in the field of study 191 «Architecture and Urban planning» of full-time education form

Methodical guidelines and tasks to a practical training and independent work are developed according to the syllabus of the course for higher education students of the first (bachelor) level in the field of study 191 “Architecture and Urban planning” of full-time education form. The purpose of a practical training is fixing of theoretical knowledge, acquisition of skills of creation of prospect in a vertical picture and also development of spatial imagination by correlation of orthogonal projections of geometrical objects and their perspective images. Methodical guidelines contain questions for independent preparation, two exercises (on 27 tasks), short methodical recommendations about their performance and the list of references. The first exercise is creation of prospect and shadows of composition from parallelepipeds. This exercise has propaedeutic character. The second exercise is the confidant to real architectural objects. Tasks are performed by a pencil in A3 format. The constructed prospects are distinguished with the reinforced line a soft pencil. Auxiliary constructions are shown with a thin line with a firm pencil. Shadows are represented by dot graphics. The results of the completed tasks are used to monitor current progress and admission to examinations

МЕТОДИЧНІ ВКАЗІВКИ та завдання до практичних занять і самостійної роботи З НАВЧАЛЬНОЇ ДИСЦИПЛІНИ «НАРИСНА ГЕОМЕТРІЯ». ЧАСТИНА 1. ОРТОГОНАЛЬНІ ПРОЕКЦІЇ для здобувачів вищої освіти першого (бакалаврського) рівня за спеціальністю 191 «Архітектура та містобудування» денної форми навчання. Methodical guidelines and tasks to a practical training and independent work in the discipline «Descriptive Geometry» for higher education students of the first (bachelor) level in the field of study 191 «Architecture and Urban planning» of full-time education form

Methodical guidelines and tasks to a practical training and independent work are developed according to the syllabus of the course for higher education students of the first (bachelor) level in the field of study 191 “Architecture and Urban planning” of full-time education form. The purpose of practical training is fixing of theoretical knowledge, acquisition of skills of the solution of the various problems of descriptive geometry arising in practice of architectural and construction design and also development of space imagination by correlation of geometrical objects in three-dimensional space with their image on the plane. Each subject consists of questions of independent training, tasks and short methodical guidelines. All tasks are performed by pencil on A3 format. Basic data and results are allocated with the reinforced line. Auxiliary constructions are shown with a thin line with a hard pencil. Results of the carried-out tasks serve for control of the current progress and admission to examinations

МЕТОДИЧНІ ВКАЗІВКИ та завдання до практичних занять і самостійної роботи З НАВЧАЛЬНОЇ ДИСЦИПЛІНИ «НАРИСНА ГЕОМЕТРІЯ». ЧАСТИНА 2. ТІНІ В ОРТОГОНАЛЬНИХ ПРОЕКЦІЯХ для здобувачів вищої освіти першого (бакалаврського) рівня за спеціальністю 191 «Архітектура та містобудування» денної форми навчання. Methodical guidelines and tasks to a practical training and independent work IN THE DISCIPLINE «DESCRIPTIVE GEOMETRY». PART 2. SHADOWS IN ORTHOGONAL PROJECTIONS for higher education students of the first (bachelor) level in the field of study 191 «Architecture and Urban planning» of full-time education form

Methodical guidelines and tasks to a practical training and independent work are developed according to the syllabus of the course for higher education students of the first (bachelor) level in the field of study 191 “Architecture and Urban planning” of full-time education form. The purpose of practical classes is to consolidate theoretical knowledge, acquire skills in building shadows on architectural forms, as well as develop spatial imagination by comparing geometric objects in three-dimensional space and their images on a plane. Methodical guidelines contain questions for independent training, tasks and exercises and short methodical guidelines. All tasks are performed in pencil on A3 format. Basic data and results are allocated with the reinforced line. Auxiliary constructions show the thin line a firm pencil. Results of the carried-out tasks serve for control of the current progress and admission to examinations

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Methods for site-specific modification of proteins should be quantitative and versatile with respect to the nature and size of the biological or chemical targets involved. They should require minimal modification of the target, and the underlying reactions should be completed in a reasonable amount of time under physiological conditions. Sortase-mediated transpeptidation reactions meet these criteria and are compatible with other labeling methods. Here we describe the expression and purification conditions for two sortase A enzymes that have different recognition sequences. We also provide a protocol that allows the functionalization of any given protein at its C terminus, or, for select proteins, at an internal site. The target protein is engineered with a sortase-recognition motif (LPXTG) at the place where modification is desired. Upon recognition, sortase cleaves the protein between the threonine and glycine residues, facilitating the attachment of an exogenously added oligoglycine peptide modified with the functional group of choice (e.g., fluorophore, biotin, protein or lipid). Expression and purification of sortase takes ∼3 d, and sortase-mediated reactions take only a few minutes, but reaction times can be extended to increase yields.National Institutes of Health (U.S.) (Grant RO1 AI08787

МЕТОДИЧНІ ВКАЗІВКИ та завдання до практичних занять і самостійної роботи З НАВЧАЛЬНОЇ ДИСЦИПЛІНИ «НАРИСНА ГЕОМЕТРІЯ». ЧАСТИНА 3. АКСОНОМЕТРІЯ для здобувачів вищої освіти першого (бакалаврського) рівня за спеціальністю 191 «Архітектура та містобудування» денної форми навчання. Methodical guidelines and tasks to a practical training and independent work IN THE DISCIPLINE «DESCRIPTIVE GEOMETRY». PART 3. AXONOMETRY for higher education students of the first (bachelor) level in the field of study 191 «Architecture and Urban planning» of full-time education form

Methodical guidelines and tasks to a practical training and independent work are developed according to the syllabus of the course for higher education students of the first (bachelor) level in the field of study 191 “Architecture and Urban planning” of full-time education form. The purpose of a practical training is fixing of theoretical knowledge, acquisition of skills of creation of an axonometry and also development of spatial imagination by correlation of orthogonal projections of a geometrical object (task) and their axonometrical images. Methodical guidelines contain questions for independent preparation, a task (26 options of buildings in orthogonal projections), short methodical recommendations about their performance and the list of references. Tasks are performed by a pencil in A3 format. The constructed axonometry are distinguished with the reinforced line a soft pencil. Auxiliary constructions are shown with a thin line with a firm pencil. Shadows are represented by dot graphics. The results of the completed tasks are used to monitor current progress and admission to examinations

Forward Physics at the LHC (Elba 2010)

The papers review the main theoretical and experimental aspects of the Forward Physics at the Large Hadron Collider

First Results from the TOTEM Experiment

The first physics results from the TOTEM experiment are here reported, concerning the measurements of the total, differential elastic, elastic and inelastic pp cross-section at the LHC energy of $\sqrt{s}$ = 7 TeV, obtained using the luminosity measurement from CMS. A preliminary measurement of the forward charged particle $\eta$ distribution is also shown.Comment: Conference Proceeding. MPI@LHC 2010: 2nd International Workshop on Multiple Partonic Interactions at the LHC. Glasgow (UK), 29th of November to the 3rd of December 201

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Transpeptidation catalyzed by ​sortase A allows the preparation of proteins that are site-specifically and homogeneously modified with a wide variety of functional groups, such as fluorophores, PEG moieties, lipids, glycans, bio-orthogonal reactive groups and affinity handles. This protocol describes immobilization of ​sortase A on a solid support (Sepharose beads). Immobilization of ​sortase A simplifies downstream purification of a protein of interest after labeling of its N or C terminus. Smaller batch and larger-scale continuous-flow reactions require only a limited amount of enzyme. The immobilized enzyme can be reused for multiple cycles of protein modification reactions. The described protocol also works with a Ca²⁺-independent variant of ​sortase A with increased catalytic activity. This heptamutant variant of ​sortase A (7M) was generated by combining previously published mutations, and this immobilized enzyme can be used for the modification of calcium-senstive substrates or in instances in which low temperatures are needed. Preparation of immobilized ​sortase A takes 1–2 d. Batch reactions take 3–12 h and flow reactions proceed at 0.5 ml h⁻¹, depending on the geometry of the reactor used.United States. National Institutes of Health (RO1 AI087879