Mumps vaccine failure investigation in Novosibirsk, Russia, 2002–2004

ABSTRACTThe aims of this study were to estimate the importance of vaccine failure (VF) in cases of mumps during 2002–2004 in the city of Novosibirsk, Western Siberia, Russia, and to genotype the responsible virus strain. Mumps virus-specific RT-PCR testing of saliva was performed for 18 cases of mumps. Sera were tested for IgM and IgG, IgG avidity, and the ability to neutralise a panel of mumps viruses, including the Leningrad-3 mumps vaccine virus. Of the 12 patients for whom vaccination status was positively determined, 11 showed serological evidence of primary VF. Sequence analysis of virus RNA amplified from saliva revealed a genotype C2 virus in 2002, a genotype H2 virus in 2003, and both genotypes in 2004. Although several vaccinated patients were positive for mumps virus IgG at the time of first sampling, only nominal levels of neutralising antibody were detected, and these were effective in neutralising the vaccine strain, but not genotype C and H mumps virus strains. These results suggest that the majority of cases of mumps in vaccinees are caused by primary VF, defined as either a lack of seroconversion or a lack of IgG maturity, as based on avidity testing. The results also support the hypothesis that sera of low neutralising antibody titre have a limited ability to neutralise heterologous mumps virus strains, suggesting that antigenic differences between circulating and mumps vaccine virus strains may play a role in cases of breakthrough infection. Consistent with previous reports, mumps virus genotypes C and H continue to circulate in Novosibirsk

Periodicities in data observed during the minimum and the rising phase of solar cycle 23; years 1996 - 1999

Three types of observations: the daily values of the solar radio flux at 7 frequencies, the daily international sunspot number and the daily Stanford mean solar magnetic field were processed in order to find all the periodicities hidden in the data. Using a new approach to the radio data, two time series were obtained for each frequency examined, one more sensitive to spot magnetic fields, the other to large magnetic structures not connected with sunspots. Power spectrum analysis of the data was carried out separately for the minimum (540 days from 1 March 1996 to 22 August 1997) and for the rising phase (708 days from 23 August 1997 to 31 July 1999) of the solar cycle 23. The Scargle periodograms obtained, normalized for the effect of autocorrelation, show the majority of known periods and reveal a clear difference between the periodicities found in the minimum and the rising phase. We determined the rotation rate of the `active longitudes' in the rising phase as equal to 444.4 $\pm$ 4 nHz (26\fd0 \pm 0\fd3). The results indicate that appropriate and careful analysis of daily radio data at several frequencies allows the investigation of solar periodicities generated in different layers of the solar atmosphere by various phenomena related to the periodic emergence of diverse magnetic structures.Comment: 15 pages, 8 figure

Glutamate Cysteine Ligase-Modulatory Subunit Knockout Mouse Shows Normal Insulin Sensitivity but Reduced Liver Glycogen Storage.

Glutathione (GSH) deficits have been observed in several mental or degenerative illness, and so has the metabolic syndrome. The impact of a decreased glucose metabolism on the GSH system is well-known, but the effect of decreased GSH levels on the energy metabolism is unclear. The aim of the present study was to investigate the sensitivity to insulin in the mouse knockout (KO) for the modulatory subunit of the glutamate cysteine ligase (GCLM), the rate-limiting enzyme of GSH synthesis. Compared to wildtype (WT) mice, GCLM-KO mice presented with reduced basal plasma glucose and insulin levels. During an insulin tolerance test, GCLM-KO mice showed a normal fall in glycemia, indicating normal insulin secretion. However, during the recovery phase, plasma glucose levels remained lower for longer in KO mice despite normal plasma glucagon levels. This is consistent with a normal counterregulatory hormonal response but impaired mobilization of glucose from endogenous stores. Following a resident-intruder stress, during which stress hormones mobilize glucose from hepatic glycogen stores, KO mice showed a lower hyperglycemic level despite higher plasma cortisol levels when compared to WT mice. The lower hepatic glycogen levels observed in GCLM-KO mice could explain the impaired glycogen mobilization following induced hypoglycemia. Altogether, our results indicate that reduced liver glycogen availability, as observed in GCLM-KO mice, could be at the origin of their lower basal and challenged glycemia. Further studies will be necessary to understand how a GSH deficit, typically observed in GCLM-KO mice, leads to a deficit in liver glycogen storage