Enhanced ribosomal association of p27Kip1 mRNA is a mechanism contributing to accumulation during growth arrest

p27(Kip1) regulates the decision to enter into S-phase or withdraw from the cell cycle by establishing an inhibitory threshold above which G(1) cyclin-dependent kinases accumulate before activation. We have used the HL-60 cell line to study regulation of p27 as cells withdraw from the cell cycle following treatment with 12-O-tetra-decanoylphorbol-13-acetate (TPA). We found that the amount of p27 is maximal in G(0) cells, lower in G(1) cells, and undetectable in S-phase cells, In contrast to the protein, the amount of p27 mRNA was the same in these populations, suggesting tliat accumulation of p27 during the cell cycle and as cells withdraw hom the cell cycle is controlled by post-transcriptional mechanisms, In S-phase cells, the degradation of p27 appears to predominate as a regulatory mechanism, In G(0) cells, there was an increase in the synthesis rate of p27, Our data demonstrate that, in G(0) cells, accumulation of p27 is due to an increase in the amount of p27 mRNA in polyribosomes

Penetrating Dust Tori in AGN

We present preliminary results from high resolution (~ 0.05") adaptive optics observations of Cygnus A. The images show a bi-conic structure strongly suggestive of an obscuring torus around a quasar nucleus. A bright (K'=18.5) point source is found near the expected position of the nucleus. We interpret this source as the hot inner rim of the torus seen through the opening of the torus. Using high angular resolution K-band spectroscopy, we measure the ratio of molecular to recombination hydrogen lines as a function of distance to the center of the putative torus. These measurements place constraints on the properties of the torus and indicate a projected diameter of ~600 pc.Comment: To appear in proceeding from "Penetrating Bars through Masks of Cosmic Dust" conference in South Africa. 5 page

Observation of the first gravitational microlensing event in a sparse stellar field : the Tago event

We report the observation of the first gravitational microlensing event in a sparse stellar field, involving the brightest (V=11.4 mag) andclosest (~ 1 kpc) source star to date. This event was discovered by an amateurastronomer, A. Tago, on 2006 October 31 as a transient brightening, by ~4.5 mag during a ~15 day period, of a normal A-type star (GSC 3656-1328) in the Cassiopeia constellation. Analysis of both spectroscopic observations and the light curve indicates that this event was caused by gravitational microlensing rather than an intrinsically variable star. Discovery of this single event over a 30 year period is roughly consistent with the expected microlensing rate for the whole sky down to V = 12 mag stars. However, the probability for finding events with such a high magnification (~ 50) is much smaller, by a factor ~1/50, which implies that the true event rate may be higher than expected. This discovery indicates the potential of all sky variability surveys, employing frequent sampling by telescopes with small apertures and wide fields of view, for finding such rare transient events, and using the observations to explore galactic disk structure and search for exo-planets.Comment: 13 pages, 2 tables, 3 figures, accepted by Ap

Rotation-Measures across Parsec-scale Jets of FRI radio galaxies

We present the results of a parsec-scale polarization study of three FRI radio galaxies - 3C66B, 3C78 and 3C264 - obtained with the Very Long Baseline Array at 5, 8 and 15 GHz. Parsec-scale polarization has been detected in a large number of beamed radio-loud active galactic nuclei, but in only a handful of the relatively unbeamed radio galaxies. We report here the detection of parsec-scale polarization at one or more frequencies in all three FRI galaxies studied. We detect Faraday rotation measures of the order of a few hundred rad/m^2 in the nuclear jet regions of 3C78 and 3C264. In 3C66B polarization was detected at 8 GHz only. A transverse rotation measure gradient is observed across the jet of 3C78. The inner-jet magnetic field, corrected for Faraday rotation, is found to be aligned along the jet in both 3C78 and 3C264, although the field becomes orthogonal further from the core in 3C78. The RM values in 3C78 and 3C264 are similar to those previously observed in nearby radio galaxies. The transverse RM gradient in 3C78, the increase in the degree of polarization at the jet edge, the large rotation in the polarization angles due to Faraday rotation and the low depolarization between frequencies, suggests that a layer surrounding the jet with a sufficient number of thermal electrons and threaded by a toroidal or helical magnetic field is a good candidate for the Faraday rotating medium. This suggestion is tentatively supported by Hubble Space Telescope optical polarimetry but needs to be examined in a greater number of sources.Comment: Accepted for publication in The Astrophysical Journal, March 2009 - 20 v694 issu

Differential effects of warming and nitrogen fertilization on soil respiration and microbial dynamics in switchgrass croplands

The mechanistic understanding of warming and nitrogen (N) fertilization, alone or in combination, on microbially mediated decomposition is limited. In this study, soil samples were collected from previously harvested switchgrass (Panicum virgatum L.) plots that had been treated with high N fertilizer (HN: 67 kg N ha−1) and those that had received no N fertilizer (NN) over a 3-year period. The samples were incubated for 180 days at 15 °C and 20 °C, during which heterotrophic respiration, δ13C of CO2, microbial biomass (MB), specific soil respiration rate (Rs: respiration per unit of microbial biomass), and exoenzyme activities were quantified at 10 different collections time. Employing switchgrass tissues (referred to as litter) with naturally abundant 13C allowed us to partition CO2 respiration derived from soil and amended litter. Cumulative soil respiration increased significantly by 16.4% and 4.2% under warming and N fertilization, respectively. Respiration derived from soil was elevated significantly with warming, while oxidase, the agent for recalcitrant soil substrate decomposition, was not significantly affected by warming. Warming, however, significantly enhanced MB and Rs indicating a decrease in microbial growth efficiency (MGE). On the contrary, respiration derived from amended litter was elevated with N fertilization, which was consistent with the significantly elevated hydrolase. N fertilization, however, had little effect on MB and Rs, suggesting little change in microbial physiology. Temperature and N fertilization showed minimal interactive effects likely due to little differences in soil N availability between NN and HN samples, which is partly attributable to switchgrass biomass N accumulation (equivalent to ~53% of fertilizer N). Overall, the differential individual effects of warming and N fertilization may be driven by physiological adaptation and stimulated exoenzyme kinetics, respectively. The study shed insights on distinct microbial acquisition of different substrates under global temperature increase and N enrichment

The magnetic field and geometry of the oblique shock in the jet of 3C 346

We investigate the brightest regions of the kpc-scale jet in the powerful radio galaxy 3C 346, using new optical HST ACS/F606W polarimetry together with Chandra X-ray data and 14.9 GHz and 22.5 GHz VLA radio polarimetry. The jet shows a close correspondence in optical and radio morphology, while the X-ray emission shows an 0.80 +/- 0.17 kpc offset from the optical and radio peak positions. Optical and radio polarimetry show the same apparent magnetic field position angle and fractional polarization at the brightest knot, where the jet undergoes a large kink of almost 70 degrees in the optical and radio images. The apparent field direction here is well-aligned with the new jet direction, as predicted by earlier work that suggested the kink was the result of an oblique shock. We have explored models of the polarization from oblique shocks to understand the geometry of the 3C 346 jet, and find that the upstream flow is likely to be highly relativistic (0.91 +0.05 / -0.07 c), where the plane of the shock front is inclined at an angle of 51 (+/- 11) degrees to the upstream flow which is at an angle 14 (+8 / -7) degrees to our line of sight. The actual deflection angle of the jet in this case is only 22 degrees.Comment: 11 pages, 5 figures. Accepted by MNRA