Loci Controlling Resistance to High Plains Virus and Wheat Streak Mosaic Virus in a B73 × Mo17 Population of Maize

High Plains disease has the potential to cause significant yield loss in susceptible corn (Zea mays L.) and wheat (Triticum aestivum L.) genotypes, especially in the central and western USA. The primary causal agent, High Plains virus (HPV), is vectored by wheat curl mite (WCM; Aceria tossicheila Keifer), which is also the vector of wheat streak mosaic virus (WSMV). In general, the two diseases occur together as a mixed infection in the field. The objective of this research was to characterize the inheritance of HPV and WSMV resistance using B73 (resistant to HPV and WSMV) × Mo17 (moderately susceptible to HPV and WSMV) recombinant inbred lines. A population of 129 recombinant inbred lines scored for 167 molecular markers was used to evaluate resistance to WSMV and to a mixed infection of WSMV and HPV. Loci conferring resistance to systemic movement of WSMV in plants mapped to chromosomes 3, 6, and 10, consistent with the map position of wsm2, wsm1, and wsm3, respectively. Major genes for resistance to systemic spread of HPV in doubly infected plants mapped to chromosomes 3 and 6, coincident or tightly linked with the WSMV resistance loci. Analysis of doubly infected plants revealed that chromosome 6 had a major effect on HPV resistance, consistent with our previous analysis of B73 × W64A and B73 × Wf9 populations. Quantitative trait loci (QTL) affecting resistance to localized symptom development mapped to chromosomes 4 (umc66), 5 (bnl5.40), and 6 (umc85), and accounted for 24% of the phenotypic variation. Localized symptoms may reflect the amount of mite feeding or the extent of virus spread at the point of infection. Identification of cosegregating markers may facilitate selection for HPV and WSMV resistance in corn breeding programs

On the Use of High-Frequency Surface Wave Oceanographic Research Radars as Bistatic Single-Frequency Oblique Ionospheric Sounders

We demonstrate that bistatic reception of high-frequency oceanographic radars can be used as single-frequency oblique ionospheric sounders. We develop methods that are agnostic of the software-defined radio system to estimate the group range from the bistatic observations. The group range observations are used to estimate the virtual height and equivalent vertical frequency at the midpoint of the oblique propagation path. Uncertainty estimates of the virtual height and equivalent vertical frequency are presented. We apply this analysis to observations collected from two experiments run at two locations in different years, but utilizing similar software-defined radio data collection systems. In the first experiment, 10 d of data were collected in March 2016 at a site located in Maryland, USA, while the second experiment collected 20 d of data in October 2020 at a site located in South Carolina, USA. In both experiments, three Coastal Oceanographic Dynamics and Applications Radars (CODARs) located along the Virginia and North Carolina coast of the US were bistatically observed at 4.53718 MHz. The virtual height and equivalent virtual frequency were estimated in both experiments and compared with contemporaneous observations from a vertical incident digisonde-ionosonde at Wallops Island, VA, USA. We find good agreement between the oblique CODAR-derived and WP937 digisonde virtual heights. Variations in the virtual height from the CODAR observations and the digisonde are found to be nearly in phase with each other. We conclude from this investigation that observations of oceanographic radar can be used as single-frequency oblique incidence sounders. We discuss applications with respect to investigations of traveling ionospheric disturbances, studies of day-to-day ionospheric variability, and using these observations in data assimilation

Chloroplast DNA and Nuclear DNA Content Variations among Cultivars of Switchgrass, \u3ci\u3ePanicum virgatum\u3c/i\u3e L.

Switchgrass, Panicum virgatum L., is a native, cross-pollinated, morphologically diverse species with an array of ploidy levels and ecotypes. Switchgrass is found throughout most of the USA and Canada, primarily east of the Rocky Mountains and south of Hudson Bay. The objective of this research was to determine if chloroplast DNA (cpDNA) restriction fragment length polymorphisms (RFLPs) occur among switchgrass cultivars and experimental strains that differ in ploidy level or ecotype classification. Eighteen switchgrass cultivars or experimental strains representative of reported ecotypes, ploidy levels, and the geographical range of switchgrass were surveyed for cpDNA polymorphisms by means of four restriction endonucleases and 20 sorghum cpDNA probes. One polymorphism was detected which was associated with the lowland-upland ecotype classification. The lowland cultivars contained a restriction site change that was not present in the upland cultivars. The two cytotypes discovered have been designated as the U (upland) or L (lowland) cytotype. The lowland cultivars had 3 pg DNA/nuclei as measured by flow cytometry while the upland types had either 3 or 6 pg DNA/nuclei. There were no cpDNA polymorphisms among the upland cultivars regardless of ploidy level as measured by DNA content. These results demonstrate that cpDNA differs among switchgrasses and that this variation is associated with ecotype variation but not with nuclear DNA content

Genetic dissection of maturity using RFLPs.

Several genetic regions having major effects on maturity have been identified using RFLP analysis. One such region on chromosomes 8 is important across several diverse genotypes and ccounts for up to 50% of the variation for maturity in a cross involving an inbred line (N28) and a 20-backcross generation derivative (N28E). In addition to the chromsome 8 QTL for maturity, we have evidence using the backcross-derived line approach for regions controlling maturity on chromosomes 1, 2, 3, 5, 7, and 9. Chromosome 5 appears to be especially important in both magnitude of effect and across several genetic hackground. Maturity as measured by days to pollen shed or silking may be controlled by additive or nearly dominant gene action depending on the chromosome region. The marker-trait linkages were consistent across environnments based on A662 X B73 F3 per se and testcross evaluations from three locations in one year. UMC12 (chromosome 8) and UMC54 (chromosome 5) also marked important regions for maturity in these materials

Current Closure in the Auroral Ionosphere: Results from the Auroral Current and Electrodynamics Structure Rocket Mission

The Auroral Current and Electrodynamics Structure (ACES) mission consisted of two sounding rockets launched nearly simultaneously from Poker Flat Research Range, AK on January 29, 2009 into a dynamic multiple-arc aurora. The ACES rocket mission was designed to observe electrodynamic and plasma parameters above and within the current closure region of the auroral ionosphere. Two well instrumented payloads were flown along very similar magnetic field footprints, at different altitudes, with small temporal separation between both payloads. The higher altitude payload (apogee 360 km), obtained in-situ measurements of electrodynamic and plasma parameters above the current closure region to determine the input signature. The low altitude payload (apogee 130 km), made similar observations within the current closure region. Results are presented comparing observations of the electric fields, magnetic components, and the differential electron energy flux at magnetic footpoints common to both payloads. In situ data is compared to the ground based all-sky imager data, which presents the evolution of the auroral event as the payloads traversed through magnetically similar regions. Current measurements derived from the magnetometers on the high altitude payload observed upward and downward field-aligned currents. The effect of collisions with the neutral atmosphere is investigated to determine it is a significant mechanism to explain discrepancies in the low energy electron flux. The high altitude payload also observed time-dispersed arrivals in the electron flux and perturbations in the electric and magnetic field components, which are indicative of Alfven waves

ADAM17 substrate release in proximal tubule drives kidney fibrosis

Kidney fibrosis following kidney injury is an unresolved health problem and causes significant morbidity and mortality worldwide. In a study into its molecular mechanism, we identified essential causative features. Acute or chronic kidney injury causes sustained elevation of a disintegrin and metalloprotease 17 (ADAM17); of its cleavage-activated proligand substrates, in particular of pro-TNFα and the EGFR ligand amphiregulin (pro-AREG); and of the substrates\u27 receptors. As a consequence, EGFR is persistently activated and triggers the synthesis and release of proinflammatory and profibrotic factors, resulting in macrophage/neutrophil ingress and fibrosis. ADAM17 hypomorphic mice, specific ADAM17 inhibitor-treated WT mice, or mice with inducible KO of ADAM17 in proximal tubule (Slc34a1-Cre) were significantly protected against these effects. In vitro, in proximal tubule cells, we show that AREG has unique profibrotic actions that are potentiated by TNFα-induced AREG cleavage. In vivo, in acute kidney injury (AKI) and chronic kidney disease (CKD, fibrosis) patients, soluble AREG is indeed highly upregulated in human urine, and both ADAM17 and AREG expression show strong positive correlation with fibrosis markers in related kidney biopsies. Our results indicate that targeting of the ADAM17 pathway represents a therapeutic target for human kidney fibrosis

Current Closure in the Auroral Ionosphere: Results from the Auroral Current and Electrodynamics Structure Rocket Mission

The Auroral Current and Electrodynamics Structure (ACES) mission consisted of two sounding rockets launched nearly simultaneously from Poker Flat Research Range, AK on January 29, 2009 into a dynamic multiple-arc aurora. The ACES rocket mission was designed to observe electrodynamic and plasma parameters above and within the current closure region of the auroral ionosphere. Two well instrumented payloads were flown along very similar magnetic field footprints, at different altitudes, with small temporal separation between both payloads. The higher altitude payload (apogee 360 km), obtained in-situ measurements of electrodynamic and plasma parameters above the current closure region to determine the input signature. The low altitude payload (apogee 130 km), made similar observations within the current closure region. Results are presented comparing observations of the electric fields, magnetic components, and the differential electron energy flux at magnetic footpoints common to both payloads. In situ data is compared to the ground based all-sky imager data, which presents the evolution of the auroral event as the payloads traversed through magnetically similar regions. Current measurements derived from the magnetometers on the high altitude payload observed upward and downward field-aligned currents. The effect of collisions with the neutral atmosphere is investigated to determine if it is a significant mechanism to explain discrepancies in the low energy electron flux. The high altitude payload also observed time-dispersed arrivals in the electron flux and perturbations in the electric and magnetic field components, which are indicative of Alfven waves

Impacts of acoustic and gravity waves on the ionosphere

The impact of regional-scale neutral atmospheric waves has been demonstrated to have profound effects on the ionosphere, but the circumstances under which they generate ionospheric disturbances and seed plasma instabilities are not well understood. Neutral atmospheric waves vary from infrasonic waves of <20 Hz to gravity waves with periods on the order of 10 min, for simplicity, hereafter they are combined under the common term Acoustic and Gravity Waves (AGWs). There are other longer period waves like planetary waves from the lower and middle atmosphere, whose effects are important globally, but they are not considered here. The most ubiquitous and frequently observed impact of AGWs on the ionosphere are Traveling Ionospheric Disturbances (TIDs), but AGWs also affect the global ionosphere/thermosphere circulation and can trigger ionospheric instabilities (e.g., Perkins, Equatorial Spread F). The purpose of this white paper is to outline additional studies and observations that are required in the coming decade to improve our understanding of the impact of AGWs on the ionosphere

Impacts of acoustic and gravity waves on the ionosphere

The impact of regional-scale neutral atmospheric waves has been demonstrated to have profound effects on the ionosphere, but the circumstances under which they generate ionospheric disturbances and seed plasma instabilities are not well understood. Neutral atmospheric waves vary from infrasonic waves of <20 Hz to gravity waves with periods on the order of 10 min, for simplicity, hereafter they are combined under the common term Acoustic and Gravity Waves (AGWs). There are other longer period waves like planetary waves from the lower and middle atmosphere, whose effects are important globally, but they are not considered here. The most ubiquitous and frequently observed impact of AGWs on the ionosphere are Traveling Ionospheric Disturbances (TIDs), but AGWs also affect the global ionosphere/thermosphere circulation and can trigger ionospheric instabilities (e.g., Perkins, Equatorial Spread F). The purpose of this white paper is to outline additional studies and observations that are required in the coming decade to improve our understanding of the impact of AGWs on the ionosphere