13 research outputs found
Measuring fluorescence into a nanofiber by observing field quadrature noise
We perform balanced homodyne detection of the electromagnetic field in a
single-mode tapered optical nanofiber surrounded by rubidium atoms in a
magneto-optical trap. Resonant fluorescence of atoms into the nanofiber mode
manifests itself as increased quantum noise of the field quadratures. The
autocorrelation function of the homodyne detector's output photocurrent
exhibits exponential fall-off with a decay time constant of ns,
which is consistent with the theoretical expectation under our experimental
conditions. To our knowledge, this is the first experiment in which
fluorescence has been observed and measured by balanced optical homodyne
detection
Discrete Routh Reduction
This paper develops the theory of abelian Routh reduction for discrete
mechanical systems and applies it to the variational integration of mechanical
systems with abelian symmetry. The reduction of variational Runge-Kutta
discretizations is considered, as well as the extent to which symmetry
reduction and discretization commute. These reduced methods allow the direct
simulation of dynamical features such as relative equilibria and relative
periodic orbits that can be obscured or difficult to identify in the unreduced
dynamics. The methods are demonstrated for the dynamics of an Earth orbiting
satellite with a non-spherical correction, as well as the double
spherical pendulum. The problem is interesting because in the unreduced
picture, geometric phases inherent in the model and those due to numerical
discretization can be hard to distinguish, but this issue does not appear in
the reduced algorithm, where one can directly observe interesting dynamical
structures in the reduced phase space (the cotangent bundle of shape space), in
which the geometric phases have been removed. The main feature of the double
spherical pendulum example is that it has a nontrivial magnetic term in its
reduced symplectic form. Our method is still efficient as it can directly
handle the essential non-canonical nature of the symplectic structure. In
contrast, a traditional symplectic method for canonical systems could require
repeated coordinate changes if one is evoking Darboux' theorem to transform the
symplectic structure into canonical form, thereby incurring additional
computational cost. Our method allows one to design reduced symplectic
integrators in a natural way, despite the noncanonical nature of the symplectic
structure.Comment: 24 pages, 7 figures, numerous minor improvements, references added,
fixed typo
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PHF6 suppresses self-renewal of leukemic stem cells in AML
Acute myeloid leukemia is characterized by uncontrolled proliferation of self-renewing myeloid progenitors accompanied by a differentiation arrest. PHF6 is a chromatin-binding protein mutated in myeloid leukemias, and its isolated loss increases mouse HSC self-renewal without malignant transformation. We report here that Phf6 knockout increases the aggressiveness of Hoxa9-driven AML over serial transplantation, and increases the frequency of leukemia initiating cells. We define the in vivo hierarchy of Hoxa9-driven AML and identify a population that we term the “LIC-e” (leukemia initiating cells enriched) population. We find that Phf6 loss expands the LIC-e population and skews its transcriptome to a more stem-like state; concordant transcriptome shifts are also observed on PHF6 knockout in a human AML cell line and in PHF6 mutant patient samples from the BEAT AML dataset. We demonstrate that LIC-e accumulation in Phf6 knockout AML occurs not due to effects on cell cycle or apoptosis, but due to an increase in the fraction of its progeny that retain LIC-e identity. Our work indicates that Phf6 loss increases AML self-renewal through context-specific effects on leukemia stem cells
Effects of proteins on the permeability of monolayers of cultured bovine arterial endothelium.
1. Monolayers of arterial endothelium on porous membranes were exposed to a pressure of 15 cmH2O at 37 degrees C, or of 30 cmH2O at 0 degree C. At constant pressure, the rate of liquid flow per unit area (Jv/A) through each monolayer decreased with time, in the way previously described for cultured endothelium. This phenomenon has been called sealing. After Jv/A stabilized, the pressure was reduced and the hydraulic permeability (Lp) of the endothelium was calculated from the relationship between Jv/A and pressure. Endothelium was seen to be damaged after some experiments at 37 degrees C, but appeared undamaged after experiments at 0 degree C. 2. Bovine serum albumin (BSA) did not influence the Lp of cultured endothelium. At 37 degrees C, the mean (+/- S.E.M.) endothelial Lp was 47.2 +/- 7.3 x 10(-7) cm s-1 cmH2O-1 (n = 10) in the presence of BSA (5 g (100 ml)-1). This is not significantly different from the mean (+/- S.E.M.) Lp of 53.4 +/- 9.0 x 10(-7) cm s-1 cmH2O-1 (n = 9) in the absence of added protein (P greater than 0.10). At 0 degree C also, there was no significant difference between mean Lps in the presence of BSA (0.1 g (100 ml)-1) and in the absence of added protein. 3. Solutions of BSA (5 g (100 ml)-1 or of the neutral polymer Ficoll 70 (4 g (100 ml)-1) did not exert any effective osmotic pressure across endothelium at 37 or 0 degrees C, respectively. 4. BSA (0.1 g (100 ml)-1) did not enable solutions of Ficoll 70 (4 g (100 ml)-1) to exert an effective osmotic pressure across endothelium at 0 degree C. 5. The mean Lp of endothelium at 0 degree C was significantly lower in the presence of cationized ferritin (CF; 0.1 g (100 ml)-1) than in the absence of added protein (P less than 0.001). Native ferritin (NF; 0.1 g (100 ml)-1) had no effect on Lp. 6. In the presence of CF (0.1 g (100 ml)-1), solutions of Ficoll 70 (4 g (100 ml)-1) exerted a mean effective osmotic pressure of 27.7 cmH2O (n = 5) across endothelium at 0 degree C. The mean effective osmotic pressure exerted across endothelium by solutions of Ficoll 70 (4 g (100 ml)-1) plus NF (0.1 g (100 ml)-1) was 1.2 cmH2O (n = 4)