Development, characterization, and use of a fetal skin cell bank for tissue engineering in wound healing.

Wound healing in fetal skin is characterized by the absence of scar tissue formation, which is not dependent on the intrauterine environment and amniotic fluid. Fetal cells have the capacity of extraordinary expansion and we describe herein the development of a fetal skin cell bank where from one organ donation (2-4 cm2) it is possible to produce several hundred million fetal skin constructs of 9 x 12 cm2. Fetal cells grow three to four times more rapidly than older skin cells cultured in the same manner and these banked fetal cells are very resistant against physical and oxidative stress when compared to adult skin cells under the same culture conditions. They are up to three times more resistant to UVA radiation and two times more resistant towards hydrogen peroxide treatment. This mechanism may be of major importance for fetal cells when they are delivered to hostile wound environments. For fetal cell delivery to patients, cells were associated with a collagen matrix to form a three-dimensional construct in order to analyze the capacity of these cells for treating various wounds. We have seen that fetal cells can modify the repair response of skin wounds by accelerating the repair process and reducing scarring in severe bums and wounds of various nature in children. Hundreds of thousands of patients could potentially be treated for acute and chronic wounds from one standardized and controlled cell bank

Detection of Ureaplasma urealyticum in Second-Trimester Amniotic Fluid by Polymerase Chain Reaction Correlates with Subsequent Preterm Labor and Delivery

Ureaplasma urealyticum is the microorganism most frequently isolated from the amniotic fluid of women in preterm labor. The relationship between intra-amniotic U. urealyticum in healthy second-trimester pregnant women and subsequent pregnancy outcome was investigated. Transabdominal amniotic fluid obtained from 254 asymptomatic women at 15-17 weeks' gestation were tested by polymerase chain reaction (PCR). U. urealyticum was identified in 29 subjects (11.4%). A subsequent preterm labor occurred in 17 U. urealyticum-positive women (58.6%), compared with 10 (4.4%) U. urealyticum-negative women (P<.0001). Preterm birth was documented in 7 (24.1%) U. urealyticum-positive women compared with only 1 U. urealyticum-negative woman (0.4%) (P<.0001). U. urealyticum-positive women also had a higher prevalence of preterm labor in a prior pregnancy (20.7%) than did the negative women (2.7%; P=.0008). PCR testing of second-trimester amniotic fluid for U. urealyticum can identify women at risk for subsequent preterm labor and deliver

The contractile properties of vaginal myofibroblasts: Is the myofibroblasts contraction force test a valuable indication of future prolapse development?

Using a specific myofibroblast contraction test, we try to predict future utero-vaginal prolapse development in young primiparae women. We compare myofibroblast cultures of the vaginal wall in primiparae women (group 1), young multiparae women (group 2) and older multiparae women (group 3) who were operated on for severe utero-vaginal prolapse. A myofibroblast-mediated collagen gel contraction assay determined a contraction factor that was compared in the three groups of women. The myofibroblasts contraction factor after 24 and 48 hours was significantly higher in group 1 women (2.4 ± 0.6/4.4 ± 1.9) compared to group 2 (1.6 ± 0.3/ 1.8 ± 0.1) andgroup 3 (1.6 ± 0.3/1.8 ± 0.3), but showed no differences in group 1 women without (2.1 ± 0.5/3.5 ± 1.9) and with (2.7 ± 0.6/5.1 ± 1.7) cystocoele. Vaginal myofibroblasts of young women show better contraction forces than young women with severe utero-vaginal prolapse. The latter have a myofibroblast contraction factor similar to those of older post-menopausal women operated for the same conditio

Ultrafast electron dynamics at metal surfaces: Competition between electron-phonon coupling and hot-electron transport

An experimental scheme (double pump/reflectivity probe using femtosecond laser pulses) enables the investigation of nonequilibrium electron dynamics at metal surfaces by measuring the equilibrated surface temperature. The competition between electron-phonon coupling and hot-electron transport gives rise to a reduced equilibrated temperature when the two pump pulses overlap in time, and provides a way of accurately determining the electron-phonon coupling constant. These observations have important consequences for femtosecond photochemical investigations

Screening for infectious diseases

INTRODUCTION: Fetal brain injury is an essential cause of lifelong morbidity. Infection appears as a cause of brain damage. Apart from chorioamnionitis, screening for infectious diseases must be considered in pregnancies with a risk of congenital infection or cases with abnormal cerebral ultrasound findings. DISCUSSION: Congenital infections include most of the major components of the TORCH complex: toxoplasmosis, rubella, cytomegalovirus, herpes, and varicella. Seronegative mothers can develop primary infection, which carries a risk of vertical transmission. The timing of the infection is a critical point, because fetal damage often depends on the gestational age at which acute maternal infection took place and occurs more likely in the first half of pregnancy. Antenatal ultrasound can detect brain abnormalities, like hydrocephalus, periventricular leukomalacia, calcifications or hemorrhage. Maternal serologic tests must be performed to look for an infectious etiology; the most frequent agents are the components of the TORCH complex. But additional serology must include parvovirus B19, HIV, and coxsackieviruses

First US Performance Measurements of Next Generation 3D USCT 2.5 Transducers

The KIT’s 3D Ultrasound Computer Tomography (USCT) II system has a multistatic setup of 2041 ultrasound transducers with approx. 1.5 MHz 6dB bandwidth and 36◦ 3 dB opening angle for 2.5 MHz. To increase the region of interest for a next USCT generation, the opening angle should be increased to approx. 60◦ and the bandwidth doubled. To increase the opening angle the size of the transducer elements was decreased to approximately half the size. A circular aperture was chosen for homogenicity of the radiation pattern in 3D. The transducer design utilizes piezo-fibres by the established Fraunhofer IMT piezo-fibre composite technology. The fibres were fabricated from PZT powder using the polysulfone spinning process. 17 fibres were positioned with a mechanical mask and filled with a matrix of epoxy. From this rod piezo composite discs were sawed and polarized. Electrodes were generated by silver-filled epoxy adhesive on the top and bottom side. Materials for acoustic backing is a Tungsten-Polyurethane composite and for acoustic matching ia aluminium oxide composite material (TMM4). Ultrasound characteristics were evaluated quantitatively with a Onda HNC-400 hydrophone in a 3-axis water tank for a randomly selected sample transducer (see Fig. a.)). Characteristics evaluated were the pressure field as function over frequency and angle in the far-field (see Fig. b.)), following the use-case. For excitation a linear encoded chirp was used, for SNR improvements averaging of measurements (64 to 256 times) was conducted. The analysis compensated for the hydrophon’s frequency and angular damping characteristics. The presented results show that the desired characteristics were mostly achieved: the 6 dB bandwidth could be vastly improved by roughly 200% (see Fig. d.)). The 6 dB pressure opening angle was approx. 50◦ (see Fig. c.)), not completly fullfilling the simulated expectations, an improvement by 31% was achieved. The results are promising for the next 3D USCT III generation

Review: ‘Gimme five’: future challenges in multiple sclerosis. ECTRIMS Lecture 2009

This article is based on the ECTRIMS lecture given at the 25th ECTRIMS meeting which was held in Düsseldorf, Germany, from 9 to 12 September 2009. Five challenges have been identified: (1) safeguarding the principles of medical ethics; (2) optimizing the risk/benefit ratio; (3) bridging the gap between multiple sclerosis and experimental autoimmune encephalitis; (4) promoting neuroprotection and repair; and (5) tailoring multiple sclerosis therapy to the individual patient. Each of these challenges will be discussed and placed in the context of current research into the pathogenesis and treatment of multiple sclerosis

Detection of Ureaplasma urealyticum in second-trimester amniotic fluid by polymerase chain reaction correlates with subsequent preterm labor and delivery

Ureaplasma urealyticum is the microorganism most frequently isolated from the amniotic fluid of women in preterm labor. The relationship between intra-amniotic U. urealyticum in healthy second-trimester pregnant women and subsequent pregnancy outcome was investigated. Transabdominal amniotic fluid obtained from 254 asymptomatic women at 15-17 weeks' gestation were tested by polymerase chain reaction (PCR). U. urealyticum was identified in 29 subjects (11.4%). A subsequent preterm labor occurred in 17 U. urealyticum-positive women (58.6%), compared with 10 (4.4%) U. urealyticum-negative women (P&lt;.0001). Preterm birth was documented in 7 (24.1%) U. urealyticum-positive women compared with only 1 U. urealyticum-negative woman (0.4%) (P&lt;.0001). U. urealyticum-positive women also had a higher prevalence of preterm labor in a prior pregnancy (20.7%) than did the negative women (2.7%; P=.0008). PCR testing of second-trimester amniotic fluid for U. urealyticum can identify women at risk for subsequent preterm labor and delivery