Phosphorus and arsenic distributions in a seasonally-stratified, iron- and manganese-rich lake: microbiological and geochemical controls

Seasonal stratification in temperate lakes greater than a few metres deep provides conditions amenable to pronounced vertical zonation of redox chemistry. Such changes are particularly evident in eutrophic systems where high phytoplankton biomass often leads to seasonally-established anoxic hypolimnia and profound changes in geochemical conditions. In this study, we investigated the behaviour of trace elements in the water column of a seasonally-stratified, eutrophic lake. Two consecutive years of data from Lake Ngapouri, North Island, New Zealand, demonstrate the occurrence of highly correlated profiles of phosphorus (P), arsenic (As), iron (Fe) and manganese (Mn), all of which increased in concentration by 1-2 orders of magnitude within the anoxic hypolimnion. Stoichiometric and mass-balance considerations demonstrate that increases in alkalinity in hypolimnetic waters were consistent with observed changes in sulfate, Fe and Mn concentrations with depth, corresponding to dissimilatory reduction of sulfate, Fe(III) and Mn(IV) hydroxides. Thermodynamic constraints on Fe, Mn and Al solubility indicate that amorphous Fe(III), Mn(IV) hydroxides most probably controlled Fe and Mn in the surface mixed layer (~0 to 8 m) while Al(III) hydroxides were supersaturated throughout the entire system. Surface complexation modelling indicated that iron hydroxides (HFO) potentially dominated As speciation in the lake. It is likely that other colloidal phases such as allophanic clays also limited HPO42- activity, reducing competition for HAsO42- adsorption to iron hydroxides. This research highlights the coupling of P, As, Fe and Mn in Lake Ngapouri, and the apparent role of multiple colloidal phases in affecting P and As activity within overarching microbiological and geochemical processes

Specimens of Somali Tales.

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We discuss the current minimisation strategies adopted by research projects involving the determination of parton distribution functions (PDFs) and fragmentation functions (FFs) through the training of neural networks. We present a short overview of a proton PDF determination obtained using the covariance matrix adaptation evolution strategy (CMA-ES) optimisation algorithm. We perform comparisons between the CMA-ES and the standard nodal genetic algorithm (NGA) adopted by the NNPDF collaboration

APFELgrid: A high performance tool for parton density determinations

We present a new software package designed to reduce the computational burden of hadron collider measurements in Parton Distribution Function (PDF) fits. The APFELgrid package converts interpolated weight tables provided by APPLgrid files into a more efficient format for PDF fitting by the combination with PDF and as evolution factors provided by APFEL. This combination significantly reduces the number of operations required to perform the calculation of hadronic observables in PDF fits and simplifies the structure of the calculation into a readily optimised scalar product. We demonstrate that our technique can lead to a substantial speed improvement when compared to existing methods without any reduction in numerical accuracy. Program Summary Program Title: APFELgrid Program Files doi: http://dx.doi.org/10.17632/mhwjt5nsg7.1 Licensing provisions: MIT license Programming language: C++ Nature of problem: Fast computation of hadronic observables under the variation of parton distribution functions. Solution method: Combination of interpolated weight grids from APPLgrid files and evolution factors from APFEL into efficient FastKernel tables. External routines/libraries: APPLgrid, APFE

Low-Reynolds-number gravity-driven migration and deformation of bubbles near a free surface

International audienceWe investigate numerically the axisymmetric migration of bubbles toward a free surface, using a boundary-integral technique. Our careful numerical implementation allows to study the bubble(s) deformation and film drainage; it is benchmarked against several tests. The rise of one bubble toward a free surface is studied and the computed bubble shape compared with the results of Princen [J. Colloid Interface Sci. 18, 178 (1963)]. The liquid film between the bubble and the free surface is found to drain exponentially in time in full agreement with the experimental work of Debre'geas et al. [Science 279, 1704 (1998)]. Our numerical results also cast some light on the role played by the deformation of the fluid interfaces and it turns out that for weakly deformed interfaces (high surface tension or a tiny bubble) the film drainage is faster than for a large fluid deformation. By introducing one or two additional bubble(s) below the first one, we examine to which extent the previous trends are affected by bubble-bubble interactions. For instance, for a 2-bubble chain, decreasing the bubblebubble separation increases the deformation of the last bubble in the chain. Finally, the exponential drainage of the film between the free surface and the closest bubble is preserved, yet the drainage is enhanced

Creation and luminescence of size-selected gold nanorods

Fluorescent metal nanoparticles have attracted great interest in recent years for their unique properties and potential applications. Their optical behaviour depends not only on size but also on shape, and will only be useful if the morphology is stable. In this work, we produce stable size-selected gold nanorods (aspect ratio 1-2) using a size-selected cluster source and correlate their luminescence behaviour with the particle shape. Thermodynamic modelling is used to predict the preferred aspect ratio of 1.5, in agreement with the observations, and confirms that the double-icosahedron observed in experiments is significantly lower in energy than the alternatives. Using these samples a fluorescence lifetime imaging microscopy study observed two photon luminescence from nanoparticle arrays and a fast decay process (<100 ps luminescence lifetime), which are similar to those found from ligand stabilized gold nanorods under the same measurement conditions, indicating that a surface plasmon enhanced two-photon excitation process is still active at these small sizes. By further reducing the nanoparticle size, this approach has the potential to investigate size-dependent luminescence behaviour at smaller sizes than has been possible before

Local and Regional Indian Summer Monsoon Precipitation Dynamics During Termination II and the Last Interglacial

To date Indian summer monsoon (ISM) dynamics have been assessed by changes in stalagmite δ18O. However, stalagmite δ18O is influenced by multiple environmental factors (e.g., atmospheric moisture transport, rainfall amount at the study site, and ISM seasonality), precluding simple and clear reconstructions of rainfall amount or variability. This study aims to disentangle these environmental factors by combining δ18O, δ44Ca, and elemental data from a stalagmite covering Termination II and the last interglacial from Mawmluh Cave, NE India, to produce a semiquantitative reconstruction of past ISM rainfall. We interpret δ18O as a mixed signal of rainfall source dynamics and rainfall amount and coupled δ44Ca and X/Ca ratios as indicators of local infiltration rate and prior calcite precipitation in the karst zone. The wettest conditions in our studied interval (135 and 100 kyrs BP; BP = before present, with the present being 1950 CE) occurred during Marine Isotope Stage 5e. Our multiproxy data set suggests a likely change in seasonal distribution of Marine Isotope Stage 5e rainfall compared to the Holocene; the wet season was longer with higher‐than‐modern dry season rainfall. Using the last interglacial as an analogue for future anthropogenic warming, our data suggest a more erratic ISM behavior in a warmer world

Characterisation of atypical enteropathogenic E. coli strains of clinical origin

BACKGROUND: Enteropathogenic E. coli (EPEC) is a prominent cause of diarrhoea, and is characterised in part by its carriage of a pathogenicity island: the locus for enterocyte effacement (LEE). EPEC is divided into two subtypes according to the presence of bundle-forming pili (BFP), a fimbrial adhesin that is a virulence determinant of typical EPEC (tEPEC), but is absent from atypical EPEC (aEPEC). Because aEPEC lack BFP, their virulence has been questioned, as they may represent LEE-positive Shiga toxin-producing E. coli (STEC) that have lost the toxin-encoding prophage, or tEPEC that have lost the genes for BFP. To determine if aEPEC isolated from humans in Australia or New Zealand fall into either of these categories, we undertook phylogenetic analysis of 75 aEPEC strains, and compared them with reference strains of EPEC and STEC. We also used PCR and DNA hybridisation to determine if aEPEC carry virulence determinants that could compensate for their lack of BFP. RESULTS: The results showed that aEPEC are highly heterogeneous. Multilocus sequence typing revealed that 61 of 75 aEPEC strains did not belong to known tEPEC or STEC clades, and of those that did, none expressed an O:H serotype that is frequent in tEPEC or STEC strains associated with disease. PCR for each of 18 known virulence-associated determinants of E. coli was positive in less than 15% of strains, apart from NleB which was detected in 30%. Type I fimbriae were expressed by all aEPEC strains, and 12 strains hybridised with DNA probes prepared from either bfpA or bfpB despite being negative in the PCR for bfpA. CONCLUSION: Our findings indicate that clinical isolates of aEPEC obtained from patients in Australia or New Zealand are not derived from tEPEC or STEC, and suggest that functional equivalents of BFP and possibly type I fimbriae may contribute to the virulence of some aEPEC strains

Direct Observation of Martensitic Phase-Transformation Dynamics in Iron by 4D Single-Pulse Electron Microscopy

The in situ martensitic phase transformation of iron, a complex solid-state transition involving collective atomic displacement and interface movement, is studied in real time by means of four-dimensional (4D) electron microscopy. The iron nanofilm specimen is heated at a maximum rate of ∼10^(11) K/s by a single heating pulse, and the evolution of the phase transformation from body-centered cubic to face-centered cubic crystal structure is followed by means of single-pulse, selected-area diffraction and real-space imaging. Two distinct components are revealed in the evolution of the crystal structure. The first, on the nanosecond time scale, is a direct martensitic transformation, which proceeds in regions heated into the temperature range of stability of the fcc phase, 1185−1667 K. The second, on the microsecond time scale, represents an indirect process for the hottest central zone of laser heating, where the temperature is initially above 1667 K and cooling is the rate-determining step. The mechanism of the direct transformation involves two steps, that of (barrier-crossing) nucleation on the reported nanosecond time scale, followed by a rapid grain growth typically in ∼100 ps for 10 nm crystallites