Stories of courage in a group of adults with Substance Use Disorder

The treatment for drug addiction is considered a difficult path for the most of patients. As matter of fact, individuals with Substance Use Disorder (SUD) experience numerous challenges before, during and after the treatment (e.g. tackling an unpredictable, uncertain and paradoxically negative future, tackling the anxiety and anticipatory fear of physical pain associated with abstinence; decide to go beyond self-justification and self-deception). Courage could be considered a positive and functional resource to help people with SUD to face challenges and difficulties related to treatment. In connection therewith, the aim of this study was to examine, using an embedded mix method analysis, the personal stories of courage of 80 individuals with SUD in order to identify the themes and types of courage used in their life. The analysis carried out showed that individuals with SUD reported more frequently stories of courage related to their SUD condition respect to other life situation. Moreover, the quantitative analysis showed that participants used more frequently psychological courage respect to moral and physical courage when these stories were referred to their SUD condition then other life situations

ka band phase locked loop oscillator dielectric resonator oscillator for satellite ehf band receiver

This paper describes the design and fabrication of a Ka Band PLL DRO having a fundamental oscillation frequency of 19.250 GHz, used as local oscillator in the low-noise block of a down converter (LNB) for an EHF band receiver. Apposite circuital models have been created to describe the behaviour of the dielectric resonator and of the active component used in the oscillator core. The DRO characterization and measurements have shown very good agreement with simulation results. A good phase noise performance is obtained by using a very highQdielectric resonator

Functional characterization of the sea urchin sns chromatin insulator in erythroid cells

Chromatin insulators are regulatory elements that determine domains of genetic functions. We have previously described the characterization of a 265 bp insulator element, termed sns, localized at the 3' end of the early historic H2A gene of the sea urchin Paracentrotus lividus. This sequence contains three cis-acting elements (Box A, Box B, and Box C+T) all needed for the enhancer-blocking activity in both sea urchin and human cells. The goal of this Study was to further characterize the sea urchin sns insulator in the erythroid environment. We employed colony assays in human (K562) and mouse (MEL) erythroid cell lines. We tested the capability of sns to interfere with the communication between the 5HS2 enhancer of the human beta-globin LCR and the gamma-globin promoter. We found that the sns sequence displays directional enhancer-blocking activity. By the use of antibodies against known DNA binding proteins, in electrophoretic mobility shift assays, we demonstrated the binding of the erythroid-specific GATA-1 and the ubiquitous Oct-1 and Sp1 transcription factors. These factors bind to Box A, Box B, and Box C+T, respectively, in both K562 and MEL nuclear extracts. These results may have significant implications for the conservation of insulator function ill evolutionary distant organisms and may prove to be of practical benefit in gene transfer applications for erythroid disorders such as hemoglobinopathies and thalassemias

Quaternary marine and continental unconformity-bounded stratigraphic units of the NW Sicily coastal belt

In the coastal sector of NW Sicily, the regional correlation of relevant unconformities recognised within the Quaternary sedimentary successions allowed the mapping of seven unconformitybounded stratigraphic units (UBSUs). The regional unconformities are marine or subaerial erosional surfaces, as well as non-depositional surfaces, locally marked by paleosoils. The erosional surfaces were produced from marine abrasion, surface water overland/ concentrated flow, river erosion, karst solution, mass movement, or wind erosion. The main lithofacies of the Quaternary UBSUs consist of: (a) marine and coastal bioclastic calcarenites, (b) aeolian sandstones, (c) river deposits, (d) colluvial deposits, (e) talus slope deposits, (f) landslide deposits, and (g) chemical carbonates (travertines and speleothems). Quaternary environmental changes, due to tectonics, climate, and sea-level oscillations, are the causes that favoured the development of erosion/deposition processes responsible for the genesis of unconformities and deposits. As a result, through the UBSU map of the NW Sicilian coastal belt, it is possible to: (i) recognise stratigraphic units controlled by tectonic, climatic, and environmental processes (and their interplay) and (ii) detect Quaternary sedimentary evolution

A Frailty Index based on clinical data to quantify mortality risk in dogs

Frailty is defined as a decline in an organism's physiological reserves resulting in increased vulnerability to stressors. In humans, a single continuous variable, the so-called Frailty Index (FI), can be obtained by multidimensionally assessing the biological complexity of an ageing organism. Here, we evaluate this variability in dogs and compare it to the data available for humans. In dogs, there was a moderate correlation between age and the FI, and the distribution of the FI increased with age. Deficit accumulation was strongly related to mortality. The effect of age, when combined with the FI, was negligible. No sex-related differences were evident. The FI could be considered in epidemiological studies and/or experimental trials to account for the potential confounding effects of the health status of individual dogs. The age-related deficit accumulation reported in dogs is similar to that demonstrated in humans. Therefore, dogs might represent an excellent model for human aging studies

MORPHOLOGICAL ANALYSIS OF ANCIENT GRAPE SEEDS FROM A SINK IN THE MIDDLE-AGE TOWN OF PALERMO

The archaeological excavations in Piazza della Vittoria, in the Roman-Middle Age town of Palermo (Sicily) put in light a sink 3.20 m deep and 1 square m. large, partially filled by thin organic sediments. Grape seeds (grape-stones), fish scales and few vertebrate bones have been found in specific strata sealed under a stratum chronologically attributed to Islamic Middle-Age period (a post-quem limit). The finding of well preserved grape seeds is peculiar and their study opens the opportunity to improve the actual knowledge about evolution, cultivation, use and trade of Vitis L. in the Mediterranean area. This preliminary work focuses on morphologic and morphometric analysis of the ancient grape seeds with two aims: i) systematically describe the remains collection and, ii) define seeds typology and a consequent morphotaxonomic attribution. Over 200 seeds have been carefully dry cleaned (soft brush), photographed and analyzed for total breadth (B), total length (L) and length of stalk (LS) parameters, the most efficient for typological attribution (1, 2); Stummer index has been also calculated (1). Apical notch length (AN) has been for the first time evaluated. Measurements on digital images have been performed using ImageJ 1.31 platform; morphological parameters have been assembled in a dedicated database. Descriptive analysis and linear correlations have been performed using SYSTAT 10. Analysis of variance (ANOVA) and Tukey’s HSD (5% level of significance, α = 0.05) have been applied. All the parameters approximate a normal distribution. Major variation has been observed in LS (c.v. = 35.6%) and AN (c.v. = 35.6%), while B and L showed a c.v. of 9.5% and 12.6% respectively. All the analyzed parameters behave as independent variables with the exception of a significant correlation between Stummer index and L (R2 = 0.45; y = 8.17-0.047x with y = L and x = Stummer index). This correlation reveals that Stummer index depends more from the L and not from the B parameter. On the base of LS measures three subgroups have been arbitrarily created in relationship with the LS: LS1 0.90 mm (35 seeds). Analyzing together the LS groups toward AN, we have found a proportional and significant correlation (p = 0.05) between the extremes LS1 and LS3. In the entire collection, Stummer index varies from 55.76 to 100.86; in the LS groups, the range is 68.38-97.87 in LS1, 61.02-100.86 in LS2 and 55.76-81.70 in LS3. A small group (17) of seeds has been excluded for the impossibility to measure the stalk. The analyzed ancient grape seeds show a wide range of variability for all the considered parameters, revealing a polymorphic collection. In general, the seeds have a rounded heart-like shape, with a noticeable pointy stalk and a very invaginated apical notch. This typical shape is more marked in LS3 group. On the base of LS measures, LS1 is ascribable to wild grapevines, while LS2 and LS3 seem to be ascribable neither to wild nor to cultivated autochthonous Vitis. Furthermore, these seeds differ from those already described in other archaeological horizons in Italy (3) and in France (2). The Stummer index varies highly, exceeding the known range of wild Vitis vinifera (4), although values close to 100 have been already found in wild grapevines in Spain (5) and values above 80 have been also described in Extra-European Vitis species (6). A deep evaluation of the sample, including isotopic analysis and aDNA studies, is in progress. 1) T. T. Korenčič, J. Jakše, Z. Korošec-Koruza (2008) Veget. Hist. Archaeobot., 17(Suppl. 1), S93-S102 2) L. Bouby, I. Figueiral, A. Bouchette, N. Rovina, S. Ivorra, T. Lacombe, T. Pastor, S. Picq, P. Marinval, J. F. Terral (2013) PLoS ONE, 8(5), e63195 3) C. Milanesi, F. Antonucci, P. Menesatti, C. Costa, C. Faleri, M. Cresti (2011) Interdisciplinaria Archaeologica – Natural Sciences in Archaeology, II(2), 95-100 4) A. Stummer (1911) Mitt. Anthropol. Gesellschaft Wien, 41, 283-296 5) F. M. De Toda, J. C. Sancha (1999) Am. J. Enol. Vitic., 50(4), 443-446 6) D. Rivera, B. Miralles, C. Obón, E. Carreño, J. A. Palazón (2007) Vitis, 46(4), 158-16

11 maggio 2011: il terremoto previsto e l’Open Day all’INGV

Fin dal 2010, un terremoto devastante era stato previsto per l’11 maggio 2011 a Roma. La previsione era stata erroneamente attribuita a Raffaele Bendandi, uno studioso autodidatta di scienze naturali, originario di Faenza e vissuto fra il 1893 e il 1979. Nei mesi precedenti, l’Istituto Nazionale di Geofisica e Vulcanologia (INGV) aveva ricevuto un notevole numero di richieste d’informazioni non solo da parte dei residenti a Roma, ma anche da parte di turisti e pendolari. Con l’approssimarsi del mese di maggio, cresceva l’attenzione della popolazione e dei media. L’INGV ha quindi deciso di organizzare un Open Day presso la propria sede di Roma per consentire al pubblico di approfondire la conoscenza del terremoto come fenomeno naturale e di avere informazioni sulla sismicità e pericolosità sismica italiana. L’Open Day è stato preceduto da una conferenza stampa, con lo scopo di presentare l’iniziativa e di avviare una discussione scientifica con i giornalisti sulla previsione dei terremoti e sul rischio sismico in Italia. Più di 30 giornalisti di quotidiani nazionali e locali, tv, agenzie di stampa e testate web hanno partecipato alla conferenza stampa e centinaia di articoli sono apparsi nei giorni successivi, pubblicizzando l’Open Day dell’11 maggio. L’INGV ha aperto la propria sede al pubblico per tutto il giorno e ha organizzato incontri con i ricercatori, visite guidate della Sala di Monitoraggio Sismico e delle mostre interattive sui terremoti e sul campo magnetico terrestre, conferenze su temi di carattere generale, quale l’impatto sociale della diffusione di voci incontrollate e la riduzione del rischio sismico. Durante la giornata sono stati inoltre inseriti sul canale YouTube/INGVterremoti 13 nuovi video per spiegare come e perché avviene un terremoto e per fornire aggiornamenti periodici sulla sismicità in Italia dalla Sala di Monitoraggio Sismico. L’11 maggio, dalle 10 del mattino alle 9 di sera, la sede INGV è stata pacificamente invasa da oltre 3000 visitatori: famiglie, scolaresche con e senza insegnanti, gruppi di protezione civile e molti giornalisti. L’iniziativa, costruita in poche settimane, ha avuto notevole risonanza ed è stata un’importante occasione per fare informazione capillare sul rischio sismico

Activity of N-Acetylcysteine Alone and in Combination with Colistin against Pseudomonas aeruginosa Biofilms and Transcriptomic Response to N-Acetylcysteine Exposure

Chronic colonization by Pseudomonas aeruginosa is critical in cystic fibrosis (CF) and other chronic lung diseases, contributing to disease progression. Biofilm growth and a propensity to evolve multidrug resistance phenotypes drastically limit the available therapeutic options. In this perspective, there has been growing interest in evaluating combination therapies, especially for drugs that can be administered by nebulization, which allows high drug concentrations to be reached at the site of infections while limiting systemic toxicity. Here, we investigated the potential antibiofilm activity of N-acetylcysteine (NAC) alone and in combination with colistin against a panel of P. aeruginosa strains (most of which are from CF patients) and the transcriptomic response of a P. aeruginosa CF strain to NAC exposure. NAC alone (8,000 mg/L) showed a limited and strain-dependent antibiofilm activity. Nonetheless, a relevant antibiofilm synergism of NAC-colistin combinations (NAC at 8,000 mg/L plus colistin at 2 to 32 mg/L) was observed with all strains. Synergism was also confirmed with the artificial sputum medium model. RNA sequencing of NAC-exposed planktonic cultures revealed that NAC (8,000 mg/L) mainly induced (i) a Zn21 starvation response (known to induce attenuation of P. aeruginosa virulence), (ii) downregulation of genes of the denitrification apparatus, and (iii) downregulation of flagellar biosynthesis pathway. NAC-mediated inhibition of P. aeruginosa denitrification pathway and flagellum-mediated motility were confirmed experimentally. These findings suggested that NAC-colistin combinations might contribute to the management of biofilm-associated P. aeruginosa lung infections. NAC might also have a role in reducing P. aeruginosa virulence, which could be relevant in the very early stages of lung colonization. © 2022 Valzano et al