Mycobacterium tuberculosis RNA Expression Patterns in Sputum Bacteria Indicate Secreted Esx Factors Contributing to Growth are Highly Expressed in Active Disease

To identify factors contributing to the ability of tubercle bacilli to grow in the lung during active infection, we analyzed RNA expression patterns in bacteria present in patient sputum. Prominent among bacterial transcripts identified were those encoding secreted peptides of the Esat-6 subfamily that includes EsxK and EsxL (Rv1197 and Rv1198). H37Rv esxKL and esxJI transcripts were differentially expressed under different growth conditions, and disruption of these genes altered growth phase kinetics in typical laboratory batch broth cultures. These growth defects, including the reduced intracellular growth of an ΔesxKL mutant in primary human macrophages, were reversed by either low multiplicity co-infection or co-culture with wild-type bacteria, demonstrating the ability of the secreted factors to rescue isogenic mutants. Complementing either only esxL or esxI alone (Rv1198 or Rv1037c) also reduced observed growth defects, indicating these genes encode factors capable of contributing to growth. Our studies indicate that the Mycobacterium tuberculosis Mtb9.9 family secreted factors EsxL and EsxI can act in trans to modulate growth of intracellular bacteria, and are highly expressed during active human lung infection

Analysis of Salmonella enterica Serotype Paratyphi A Gene Expression in the Blood of Bacteremic Patients in Bangladesh

Salmonella enterica serotype Paratyphi A is a significant and emerging global public health problem and accounts for one fifth of all cases of enteric fever in many areas of Asia. S. Paratyphi A only infects humans, and the lack of an appropriate animal model has limited the study of S. Paratyphi A infection. In this study, we report the application of an RNA analysis method, Selective Capture of Transcribed Sequences (SCOTS), to evaluate which S. Paratyphi A genes are expressed directly in the blood of infected humans. Our results provide insight into the bacterial adaptations and modifications that S. Paratyphi A may need to survive within infected humans and suggest that similar approaches may be applied to other pathogens in infected humans and animals

Design And Fabrication Of Vertical Axis Wind Turbine

Wind turbines are considered to be only 59% efficient (Ref.: Bets ,law) ,and more over with large rotor a large area wake formations means that spacing between two turbines has to be kept very large. Hence the conventional method of wind power generation has to be concluded again with the innovative approach. The VAWT with vertex turbine is one such concept that uses the principle of generating an added vortex using a two vortex generators, in order to improve the performance of the turbine this work will contain the design and development of a vortex chamber using ModelingSoftware and scaled the working model which will demonstrate the electricity generation and testing will be done on the same to determine the effect of wind speed, turbine speed, voltage, current and power generated bythe Prototype mode

Effect of geometrical properties on strength of externally prestressed steel–concrete composite beams

A parametric study was carried out to investigate the structural behaviour of composite steel-concrete T-beams under different prestressing conditions. The studied parameters include different cases of loading, tendon profiles, beam spans, initial prestressing levels and different dimensions of steel sections and concrete deck. The studied beams were modelled by the finite-element software Ansys. The effect of three geometrical parameters was investigated for three different tendon profiles. It was found that straight tendon profiles are more appropriate for beams under distributed loads, whereas a draped tendon profile is more convenient for beams under concentrated loads. In addition, the ratio of the tension flange area to the compression flange area is the most effective geometrical parameter on the ultimate resistance of the studied prestressed beams. For instance, increasing this ratio from 1 to 3 resulted in increasing the additional resistance of the beam due to prestressing from 15·4 to 46·1%. For composite beams, the presence of the concrete slab prevented the lateral-torsional buckling and accordingly minimised the effect of the span. Moreover, increasing the slab thickness-to-width ratio resulted in enhancing the average increase in beam strength from 13·5 to 19·9%