lntrogression of Bacterial Leaf Blight Resistance Gene from Oryza Minuta J.B. Presl. Ex C. B. Presl. into New Rice Type (Oryza sativa L.)

F1 Hybrids, backcross progenies, advanced introgression lines (2n=24) and monosomic alien addition lines or MAALs (2n=25) were successfully produced following embryo rescue between an elite new plant type (NPT) breeding line of Oryza sativa (2n=24, AA) and a wild species, O. minuta (2n=48, BBCC). F1 hybrids performance were intermediate between the parents. The F1 hybrids had 36 chromosomes indicating having 12 chromosome A from O. sativa and 12 B and 12 C from O. minuta. THE BACK CROSS progenies had different chromosome number indicating abnormal meiosis of the hybrids and back cross progenies. Plant with 2n=24 and 25 chromosomes were obtained in BC4FI. The hybrids and backcross progenies were susceptible to bacterial leaf blight (BB). However, several of the 2n=24 plants derived resistant plant to bacterial leaf blight race 1 of the Philippines races. The gene is different from introgressed gene intio rice from O. longistaminata ( Xa21) and from O. Minuta Acc. 101141   I Key words: Oryza minuta, MAALS, Bacterial leaf bligh

CORRELATION AND PATH ANALYSIS ON PHENOTYPIC CHARACTERS OF DOUBLED HAPLOID RICE LINES

Grain yield improvement is the main objective in rice breeding programs. The objective of this study was to determine the phenotypic characters that have a direct or indirect effect on grain yield of double haploid lines. It expected can be used as selection criteria. The study was conducted on August 2009 at Cikeumeuh Bogor using randomized block design (RBD) with three replications. The materials used were 33 doubled haploid rice lines and three varieties/landraces rice. The results showed that number of productive tillers per hill, number of grains per panicle and 1,000 grain weight of rice can be used as selection criteria in improving grain yield. These three characters has a positive and very signifi cant correlation, positive and high direct effect on grain yield, and havea high heritability value

Pembentukan Galur Haploid Ganda Padi Gogo dengan Sifat-Sifat Tipe Baru melalui Kultur Antera

The breeding of upland rice with New Plant Type characters in relatively short time can be done by using anther culture technique. The technique has been recognized as a rapid and efficient technology for crop improvement. Plant materials used in this research were F1 crossing P1 (Fatmawati x Way Rarem), P2 (Fatmawati x SGJT-28),  P3 (Fatmawati x SGJT-36), P4  (Way Rarem x Fatmawati), P5 (SGJT-28 x Fatmawati), and P6  (SGJT-36 x Fatmawati). Media for calli induction (N6) and regeneration (MS) were according to Dewi methods (2003). The results of this study indicated that P3 (Fatmawati x SGJT-36) and P6 (SGJT-36 x Fatmawati)  from resiprocal crosses gave better response in anther culture than the others crosses for their calli induction and green plant regeneration. From the six F1 crosses, 348 (53.5%) spontaneous doubled haploid (DH) pure lines were obtained, six lines from Farmawati x Way Rarem, 13 lines from Fatmawati x SGJT-28, 187 lines from Fatmawati x SGJT-36, three lines from Way Rarem x Fatmawati, five lines from SGJT-28 x Fatmawati, and 134 lines from SGJT-36 x Fatmawati.  These lines should be evaluated and characterized for their agronomical and morphological  traits.    Key words: Anther culture, doubled haploid, callus induction, green plant regeneration       

Anther Culture Ability from Crossess Between Upland and New Plant Types of Rice

Anther culture provides rapid route in obtaining pure lines in a single generation through producing green haploid plants that may be spontaneously doubled. This technique has been used for crop improvement especially in rice. The objective of this research was to determine regeneration ability of eight F1s derived from crossess between upland and new plant types of rice and from their four parents through anther culture. Completely randomized design with 25 replications was used in this research. Treatments consisted of four parent lines/varieties i.e. P1 (Fatmawati and BP360E-MR-79-2), P2 (Fulan Telo Gawa and Fulan Telo Mihat) and eight F1s obtained from reciprocal crosses of P1 and P2. Callus induction medium was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1  kinetin + 10-3  M Putrescine, while regeneration medium was based on MS + 0.5 mg L-1  NAA + 2.0 mg L-1  kinetin + 10-3  M Putrescine. The result indicated that F1 derived from Fatmawati x Fulan Telo Gawa (5.00% green plants per total anther) and their reciprocal (3.80% green plants per total anther) crosses were the most responsive genotypes in rice anther culture (had high anther culture ability). The F1 genotypes were more effective to produce green and doubled haploid plants in rice anther culture than their parents. From this research, 161 double haploid plants (29.81%) from total acclimated green plantlets were obtained.  Keywords: anther culture, upland rice, new plant type of ric