Distribution of arbuscular mycorrhizal fungi in sugarcane rhizosphere from various agricultural management practices in Northeast, Thailand

Sugarcane (Saccharum spp.) is one of the economically most important crops in Thailand. Sugarcane forms symbiotic associations with arbuscular mycorrhizal fungi (AMF). Species diversity of and root colonization by AMF may vary by agricultural management and soil properties. The objective of this study was to investigate the community composition of AMF in sugarcane rhizosphere soil with various cultivation practices. Twelve sugarcane rhizosphere soils were collected from sugarcane fields in three provinces (Nakhon Ratchasima, Khon Kaen, and Buri Ram) with various forms of agricultural management, including organic farming (OM), semi-organic farming (SM), and conventional farming with mineral fertilizers (CM). The results showed that root colonization ranged between 10 and 22%, while spore density ranged from 11 to 168 spores 100 g soil-1. Based on morphological identification of AMF, a total of 43 taxa, representing 11 genera, were observed, viz. the genera Acaulospora, Claroideoglomus, Dentiscutata, Diversispora, Entrophospora, Funneliformis, Gigaspora, Glomus, Racocetra, Rhizophagus, and Septoglomus. One unidentified species was found. The dominant genera were Acaulospora and Glomus, which were found in all sites. Diversispora pustulata was the most widely distributed species, isolated in 75% of the sites. Species diversity of AMF, expressed by Shannon–Wiener index of diversity (H’), ranged from 1.03 to 2.14 with the highest diversity in OM systems and lowest diversity in CM systems. Our results may be used for considerations of agricultural management practices to benefit from communities of native AMF, which could be important for sustainable production of sugarcane

Species richness and composition of arbuscular mycorrhizal fungi occurring on eucalypt trees (Eucalyptus camaldulensis Dehnh.) in rainy and dry season

River red gum (Eucalyptus camaldulensis Dehnh.), the most commonly planted eucalypt species globally, has several advantages and is widely used for many purposes, which makes the tree important. Mycorrhizal establishment in eucalyptus has been known for many years, and the benefits of this symbiosis have been commercially explored. The main goal of this research was to assess the diversity and distribution of arbuscular mycorrhizal fungi (AMF) on eucalyptus planted in agricultural fields in the rainy and dry season. Fields were chosen in ten different sites located in four provinces in the northeast of Thailand. Rhizosphere soil and root samples were collected and the number of AMF spores and AMF root colonization were assessed. The number of AMF spores was higher in the rainy season than in the dry season, while AMF root colonization was higher in the dry season than in the rainy season. On the basis of morphological identification of AMF, a total of 35 AMF fungal taxa in eight genera were identified, ten belonging to Acaulospora, one to Dentiscutata, one to Entrophospora, 16 to Glomus, three to Gigaspora, one to Racocetra, two to Scutellospora, and one to Septoglomus. Glomus was the dominant genus followed by Acaulospora. Relative abundance, and frequency of occurrence were higher in the rainy season than in the dry season. Racocetra fulgida was the most common species with a frequency of occurrence of 90% in rainy season, and 80% in dry season. Species richness, Simpson's index of dominance and Shannon-Wiener index of diversity were not significantly different between both seasons

Hospitalization and cost after switching from atypical to typical antipsychotics in schizophrenia patients in Thailand

Tuanthon Boonlue,1,2 Suphat Subongkot,1,2 Piyameth Dilokthornsakul,3,4 Ronnachai Kongsakon,5 Oraluck Pattanaprateep,6 Orabhorn Suanchang,7 Nathorn Chaiyakunapruk3,8–10 1Clinical Pharmacy Division, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand; 2The College of Pharmacotherapy of Thailand, Nonthaburi, Thailand; 3Center of Pharmaceutical Outcomes Research, Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok, Thailand; 4Center for Pharmaceutical Outcomes Research, University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences, Aurora, CO, USA; 5Department of Psychiatry, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand; 6Department of Health Informatics, Ramathibodi Hospital, Mahidol University, 7Department of Pharmacy, Somdet Chaopraya Institute of Psychiatry, Bangkok, Thailand; 8School of Pharmacy, Monash University Malaysia, Selangor, Malaysia; 9School of Population Health, University of Queensland, Brisbane, Australia; 10School of Pharmacy, University of Wisconsin-Madison, Madison, WI, USA Background: Several clinical practice guidelines suggest using atypical over typical antipsychotics in patients diagnosed with schizophrenia. Nevertheless, cost-containment policy urged restricting usage of atypical antipsychotics and switching from atypical to typical antipsychotics. Objective: This study aimed to evaluate clinical and economic impacts of switching from atypical to typical antipsychotics in schizophrenia patients in Thailand. Methods: From October 2010 through September 2013, a retrospective cohort study was performed utilizing electronic database of two tertiary hospitals. Schizophrenia patients aged 18 years or older and being treated with atypical antipsychotics were included. Patients were classified as atypical antipsychotic switching group if they switched to typical antipsychotics after 180 days of continual atypical antipsychotics therapy. Outcomes were schizophrenia-related hospitalization and total health care cost. Logistic and Poisson regression were used to evaluate the risk of hospitalization, and generalized linear model with gamma distribution was used to determine the health care cost. All analyses were adjusted by employing propensity score and multivariable analyses. All cost estimates were adjusted according to 2013 consumer price index and converted to US$at an exchange rate of 32.85 Thai bahts/US$. Results: A total of 2,354 patients were included. Of them, 166 (7.1%) patients switched to typical antipsychotics. The adjusted odds ratio for schizophrenia-related hospitalization in atypical antipsychotic switching group was 1.87 (95% confidence interval [CI] 1.23–2.83). The adjusted incidence rate ratio was 2.44 (95% CI 1.57–3.79) for schizophrenia-related hospitalizations. The average total health care cost was lower in patients with antipsychotic switching (–$64; 95$459 to $332). Conclusion: Switching from atypical to typical antipsychotics is associated with an increased risk of schizophrenia-related hospitalization. Nonetheless, association with average total health care cost was not observed. These findings can be of use as a part of evidence in executing prospective cost-containment policy. Keywords: antipsychotic switching, schizophrenia, hospitalization, cost, atypical antipsychotics, typical antipsychotic

A halotolerant laccase from Chaetomium strain isolated from desert soil and its ability for dye decolourization

13 p.-6 fig.-3 tab.A novel fungal laccase produced by the ascomycete Chaetomium sp. isolated from arid soil was purified and characterized and its ability to remove dyes was determined. Extracellular laccase was purified 15-fold from the crude culture to homogeneity with an overall yield of 50% using ultrafiltration and anion-exchange chromatography. The purified enzyme was found to be a monomeric protein with a molecular mass of 68 kDa, estimated by SDS-PAGE, and with an isoelectric point of 5.5. The optimal temperature and pH value for laccase activity toward 2,6-DMP were 60 °C and 3.0, respectively. It was stable at temperatures below 50 °C and at alkaline conditions. Kinetic study showed that this laccase showed higher affinity on ABTS than on 2,6-DMP. Its activity was enhanced by the presence of several metal ions such as Mg2+, Ca2+ and Zn2+, while it was strongly inhibited by Fe2+, Ag+ and Hg2+. The novel laccase also showed high, remarkable sodium chloride tolerance. Its ability to decolorize different dyes, with or without HBT (1-hydroxy-benzotriazole), as redox mediator, suggests that this protein may be useful for different industrial applications and/or bioremediation processes.Peer reviewe