88 research outputs found

    Human cathepsin D.

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    Präparative gewinnung hochgereinigter lysosomenenzyme aus rattenlebern

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    RésuméBy means of a relatively simple procedure lysosomal enzymes from rat livers with 20% yield and a 50-fold enrichment were prepared. Density gradient centrifugation or tissue-preloading with triton VR 1339 is not necessary. Up to several liters of 50% liver homogenate may be used. Intact lysosomes are not obtained, because the crucial step in the tissue fractionation scheme is the selective release of lysosomal enzymes from a crude lysosome pellet by water extraction. The presence of neutral endopeptidases with high specific activities in lysosomes (estimated using azocasein and other proteins as substrates) is demonstrated
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