Kualitas Soal Penilaian Akhir Semester (PAS) Buatan Guru Mata Pelajaran Kimia Kelas X SMK Jurusan Teknologi dan Rekayasa di Kabupaten Kotawaringin Timur pada Semester Ganjil Tahun Ajaran 2018/2019

Penelitian ini bertujuan untuk mengetahui dan mendeskripsikan tingkat kualitas soal PAS buatan guru mata pelajaran kimia kelas X SMK Jurusan Teknologi dan Rekayasa di Kabupaten Kotawaringin Timur pada semester ganjil tahun ajaran 2018/2019. Penelitian ini termasuk penelitian deskriptif. Subjek penelitian adalah soal buatan guru dan jawaban siswa hasil ulangan akhir semester. Teknik pengumpulan data dalam penelitian ini adalah metode dokumentasi. Analisis data dilakukan dengan menggunakan teknik analisis logical review dan empirical review. Hasil penelitian analisis logical review tingkat kesesuaian butir soal dengan indikator dalam silabus K13 sebesar 97,48%, dengan aspek materi, konstruksi dan bahasa sebesar 98,72%. Distribusi tingkat kognitif taksonomi bloom 52 (55%) soal kognitif C1, 23 (24%) soal kognitif C2, 17 (18%) soal kognitif C3, 3 (3%) soal tingkat C4, kesesuaian dengan kompetensi dasar sebesar 69,5%. Analisis secara empirical review reliabilitas 0,570 (cukup),  daya beda 75,7% soal baik, tingkat kesukaran butir soal 43,3% kategori sedang dan efektifitas distraktor 76,42% efektif

Pengaruh Kepemimpinan Partisipatif Terhadap Perilaku Kerja Pegawai Pada Dinas Penanaman Modal ESDM Dan Transmigrasi Provinsi Gorontalo

Penelitian ini bertujuan untuk mengetahui Pengaruh Kepemimpinan Partisipatif Terhadap Perilaku Kerja Pegawai. Metode penelitian yang digunakan dalam penelitian ini adalah metode kuantitatif dengan pendekatan kuantitatif deskriptif. Data yang digunakan adalah data primer yang diperoleh dari penyebaran kuesioner kepada pegawai di Dinas Penanaman Modal ESDM dan Transmigrasi Provinsi Gorontalo. Penarikan sampel dalam penelitian ini menggunakan rumus Slovin sehingga jumlah responden berjumlah 57. Analisis data dalam penelitian ini menggunakan analisis regresi linier sederhana dengan bantuan program SPSS 21. Hasil penelitian menunjukkan kepemimpinan partisipatif berpengaruh positif terhadap perilaku kerja pegawai. Koefisien determinasi dalam penelitian ini sebesar 55,4% yang berarti variabilitas perilaku kerja pegawai dapat dijelaskan oleh kepemimpinan partisipatif sebesar 55,4%. Sementara 44,6% dipengaruhi oleh variabel lain seperti kepemimpinan transformasional, lingkungan kerja, budaya organisasi, dan lain-lain. Dengan demikian, maka hipotesis teruji kebenarannya dan dapat diterima

A cruise ship emergency medical evacuation triggered by handheld ultrasound findings and directed by tele-ultrasound

Cruise ships travel far from shoreside medical care and present a unique austere medical environment. For the cruise ship physician, decisions regarding emergency medical evacuation can be challenging. In the event that a passenger or crew member becomes seriously ill or is injured, the use of point-of-care ultrasound may assist in clarifying the diagnosis and stratifying the risk of a delayed care, and at times expedite an emergent medical evacuation. In this report we present the first case reported in the literaturę of an emergency medical evacuation from a cruise ship triggered by handheld ultrasound. A point-of-care ultrasound performed by a trained cruise ship physician, reviewed by a remote telemedical consultant with experience in point-of-care ultrasound, identified an ectopic pregnancy with intraabdominal free fluid in a young female patient with abdominal pain and expedited emergent helicopter evacuation from a cruise ship to a shoreside facility, where she immediately underwent successful surgery. The case highlights a medical evacuation that was accurately triggered by utilising a handheld ultrasound and successfully directed via a tele-ultrasound consultation. American College of Emergency Physicians (ACEP) health care guidelines for cruise ship medical facilities should be updated to include guidelines for point-of-care ultrasound, including training and telemedical support

Surveillance of patulin in apple, grapes, juices and value-added products for sale in Pakistan

The prime objective of the current study was to investigate the occurrence of mycotoxin patulin (PAT) in apples, grapes and their value added products. PAT was determined by a validated method based on HPLC with UV detector. A total of 381 samples comprising apple and grape fruits (n = 133 each), apple-based products (n = 76, juice, puree, jam) and grape juice (n = 39) were analyzed. PAT was found in 58.9% samples of apple and apple-based products, with a mean of 49.8 µg/kg (maximum 396 µg/kg), while 27.3% samples contained PAT beyond the maximum regulatory limit of 50 µg/kg. The average levels of PAT contamination in apple-derived products was higher in apple juice concentrate, followed by apple puree, apple juice and apple jam. The incidence of PAT in table grapes was 65.1%, with a mean of 53.9 µg/kg (maximum 505 µg/kg), whereas 23.8% exceeded the maximum level. Among the fruit samples, there were differences in PAT contents due to apple variety (6 types) or grape variety (8 types), as well as for sampling location. Our investigations showed the wide PAT occurrence in fruits and derived value-added products affecting consumer product safety, so that the population is chronically exposed to this toxin

A limited survey of aflatoxins and zearalenone in feed and feed ingredients from Pakistan

This work presents current information on the presence of aflatoxins (AFs) and zearalenone (ZEN) in feed and feed ingredients from Punjab, Pakistan. The 105 samples tested were concentrated feed, i.e., cotton seed meal (18 samples) and soybean meal (14), and feed ingredients, i.e., crushed corn (17), crushed wheat (15), barley (17). and poultry feed (24). Samples were analyzed using high-performance liquid chromatography equipped with a fluorescence detector. Analysis revealed that 69 of 105 samples were contaminated with AFs, and the highest mean concentrations of AFB1 (6.20 μg/kg) and total AFs (9.30 μg/kg) were found in poultry feed samples. The mean total AF concentrations ranged from the limit of quantification to 165.5 μg/kg. However, 75 of the 105 samples were positive for ZEN. The highest mean concentration (19.45 μg/kg) was found in poultry feed samples. The mean ZEN concentrations were 0.15 to 145.30 μg/kg. The prevalence of AFs and ZEN was high in feed and feed ingredients and needs urgent attention

HnRNP K mislocalisation is a novel protein pathology of frontotemporal lobar degeneration and ageing and leads to cryptic splicing

Heterogeneous nuclear ribonucleoproteins (HnRNPs) are a group of ubiquitously expressed RNA-binding proteins implicated in the regulation of all aspects of nucleic acid metabolism. HnRNP K is a member of this highly versatile hnRNP family. Pathological redistribution of hnRNP K to the cytoplasm has been linked to the pathogenesis of several malignancies but, until now, has been underexplored in the context of neurodegenerative disease. Here we show hnRNP K mislocalisation in pyramidal neurons of the frontal cortex to be a novel neuropathological feature that is associated with both frontotemporal lobar degeneration and ageing. HnRNP K mislocalisation is mutually exclusive to TDP-43 and tau pathological inclusions in neurons and was not observed to colocalise with mitochondrial, autophagosomal or stress granule markers. De-repression of cryptic exons in RNA targets following TDP-43 nuclear depletion is an emerging mechanism of potential neurotoxicity in frontotemporal lobar degeneration and the mechanistically overlapping disorder amyotrophic lateral sclerosis. We silenced hnRNP K in neuronal cells to identify the transcriptomic consequences of hnRNP K nuclear depletion. Intriguingly, by performing RNA-seq analysis we find that depletion of hnRNP K induces 101 novel cryptic exon events. We validated cryptic exon inclusion in an SH-SY5Y hnRNP K knockdown and in FTLD brain exhibiting hnRNP K nuclear depletion. We, therefore, present evidence for hnRNP K mislocalisation to be associated with FTLD and for this to induce widespread changes in splicing

Evaluation of the Intestinal Bacterial Community of Local Omani and Cobb 500 Broiler Chickens Raised in an Open-Sided House Using 16S rDNA-Based Analysis

Little is known about how the intestinal bacterial microbiota differs among different strains of chickens raised in an open sided house, predominantly those with lower growth rates, such as Indigenous chickens. Ninety-one-day-old chicks of each strain of chickens were raised in an open-sided house system and fed a conventional corn-soybean meal diet from Day 0–35 days of age. The objective of this study was to assess the relative abundance of bacteria microbiota identified in the intestinal tract of local Omani and Cobb 500 broiler chickens raised in an open-sided house system using 16S rDNA-based analysis. The results obtained showed the diversity of bacterial populations in different intestinal regions of two chicken strains. Bacilli were found in higher numbers and reached 98.8% of the bacteria in the duodenum on Day 5 in Cobb 500 versus 72.5% in the Omani chickens. Local Omani chickens had significantly higher numbers of Clostridia at an early age period. On Day 5 Clostridia comprised 13.1% of the bacteria in the duodenum of local Omani chickens, versus only 0.062% in the Cobb 500. The relative abundance of the bacterial microbiota differed significantly (p <0.05) across different intestinal segments of the two strains of chickens, suggesting that each region generated its bacterial community with different relative abundances