Analysis of the Effect of Intestinal Ischemia and Reperfusion on the Rat Neutrophils Proteome

Intestinal ischemia and reperfusion injury is a model system of possible consequences of severe trauma and surgery, which might result into tissue dysfunction and organ failure. Neutrophils contribute to the injuries preceded by ischemia and reperfusion. However, the mechanisms by which intestinal ischemia and reperfusion stimulate and activate circulating neutrophils is still not clear. In this work, we used proteomics approach to explore the underlying regulated mechanisms in Wistar rat neutrophils after ischemia and reperfusion. We isolated neutrophils from three different biological groups; control, sham laparotomy, and intestinal ischemia/reperfusion. In the workflow, we included iTRAQ-labeling quantification and peptide fractionation using HILIC prior to LC-MS/MS analysis. From proteomic analysis, we identified 2,045 proteins in total that were grouped into five different clusters based on their regulation trend between the experimental groups. A total of 417 proteins were found as significantly regulated in at least one of the analyzed conditions. Interestingly, the enzyme prediction analysis revealed that ischemia/reperfusion significantly reduced the relative abundance of most of the antioxidant and pro-survival molecules to cause more tissue damage and ROS production whereas some of the significantly up regulated enzymes were involved in cytoskeletal rearrangement, adhesion and migration. Clusters based KEGG pathways analysis revealed high motility, phagocytosis, directional migration, and activation of the cytoskeletal machinery in neutrophils after ischemia and reperfusion. Increased ROS production and decreased phagocytosis were experimentally validated by microscopy assays. Taken together, our findings provide a characterization of the rat neutrophil response to intestinal ischemia and reperfusion and the possible mechanisms involved in the tissue injury by neutrophils after intestinal ischemia and reperfusion

Construction of Quantum Target Space from World-Sheet States using Quantum State Tomography

In this paper, we will construct the quantum states of target space coordinates from world-sheet states, using quantum state tomography. To perform quantum state tomography of an open string, we will construct suitable quadrature operators. We do this by first defining the quadrature operators in world-sheet, and then using them to construct the quantum target space quadrature operators for an open string. We will connect the quantum target space to classical geometry using coherent string states. We will be using a novel construction based on a string displacement operator to construct these coherent states. The coherent states of the world-sheet will also be used to construct the coherent states in target space.Comment: 13 pages, 4 figures, 1 appendi

A Novel Application of Quantum Speed Limit to String Theory

In this work, we investigate the implications of the concept of quantum speed limit in string field theory. We adopt a novel approach to the problem of time on world-sheet based on Fisher information, and arrive at a minimum time for a particle state to evolve into another particle state. This is done using both the Mandelstam-Tamm bound and the Margolus-Levitin bound. This implies that any interaction has to be smeared over such an interval, and any interaction in the effective quantum field theory has to be non-local. As non-local quantum field theories are known to be finite, it is expected that divergences should be removed from effective quantum field theories due to the quantum speed limit of string theory.Comment: 9 page

Phosphoproteomic analysis of rat neutrophils shows the effect of intestinal ischemia/reperfusion and preconditioning on kinases and phosphatases

Intestinal ischemia reperfusion injury (iIRI) is a severe clinical condition presenting high morbidity and mortality worldwide. Some of the systemic consequences of IRI can be prevented by applying ischemic preconditioning (IPC), a series of short ischemia/reperfusion events preceding the major ischemia. Although neutrophils are key players in the pathophysiology of ischemic injuries, neither the dysregulation presented by these cells in iIRI nor the protective effect of iIPC have their regulation mechanisms fully understood. Protein phosphorylation, as well as the regulation of the respective phosphatases and kinases are responsible for regulating a large number of cellular functions in the inflammatory response. Moreover, in previous work we found hydrolases and transferases to be modulated in iIR and iIPC, suggesting the possible involvement of phosphatases and kinases in the process. Therefore, in the present study, we analyzed the phosphoproteome of neutrophils from rats submitted to mesenteric ischemia and reperfusion, either submitted or not to IPC, compared to quiescent controls and sham laparotomy. Proteomic analysis was performed by multi-step enrichment of phosphopeptides, isobaric labeling, and LC-MS/MS analysis. Bioinformatics was used to determine phosphosite and phosphopeptide abundance and clustering, as well as kinases and phosphatases sites and domains. We found that most of the phosphorylation-regulated proteins are involved in apoptosis and migration, and most of the regulatory kinases belong to CAMK and CMGC families. An interesting finding revealed groups of proteins that are modulated by iIR, but such modulation can be prevented by iIPC. Among the regulated proteins related to the iIPC protective effect, Vamp8 and Inpp5d/Ship are discussed as possible candidates for control of the iIR damage

Poly(Acrylic acid)–Based Hybrid Inorganic–Organic Electrolytes Membrane for Electrical Double Layer Capacitors Application

Nanocomposite polymer electrolyte membranes (NCPEMs) based on poly(acrylic acid)(PAA) and titania (TiO2) are prepared by a solution casting technique. The ionic conductivity of NCPEMs increases with the weight ratio of TiO2.The highest ionic conductivity of (8.36 ± 0.01) × 10−4 S·cm−1 is obtained with addition of 6 wt % of TiO2 at ambient temperature. The complexation between PAA, LiTFSI and TiO2 is discussed in Attenuated total reflectance-Fourier Transform Infrared (ATR-FTIR) studies. Electrical double layer capacitors (EDLCs) are fabricated using the filler-free polymer electrolyte or the most conducting NCPEM and carbon-based electrodes. The electrochemical performances of fabricated EDLCs are studied through cyclic voltammetry (CV) and galvanostatic charge-discharge studies. EDLC comprising NCPEM shows the specific capacitance of 28.56 F·g−1 (or equivalent to 29.54 mF·cm−2) with excellent electrochemical stability