Investigating the Role of the Nucleosome Remodeling Factor NURF as a Regulator of Gene Expression

The nucleosome remodeling factor (NURF) is an evolutionary conserved ATP-dependent chromatin remodeling factor. It was first isolated from Drosophila as a complex with enzymatic activity that once recruited to nucleosome, it slides the nucleosome to provide accessibility for transcription factors. Since then, numerous works from animal models and cell lines show the role of NURF as a regulator of gene expression. NURF interacts with H3K4me3 and sequence specific transcription factors that recruit the complex to promoter regions. Whether this is the only mechanism by which NURF regulates gene expression is not known. However, other ATP-dependent chromatin remodeling complexes are known to regulate gene expression independent from transcription initiation. In order to explore the role of NURF in regulating gene expression, we utilized two genome wide approaches to map NURF binding and NURF dependent changes in chromatin structure using ChIP-Seq and FAIRE-Seq, respectively. From these analyses, we discovered that NURF broadly localizes in the genome with preferences to gene bodies and 3’ends of genes. Also, we found that NURF maintains open chromatin regions at upstream, intron and downstream of genes. These novel findings shed light on new roles for NURF complex within genes, in addition to its classical role at promoter regions. Furthermore, we discovered the function of a previously uncharacterized domain in the NURF specific subunit BPTF. We show that the N-terminal the plant homeodomain (PHD) of BPTF directly interacts with THOC4, a protein associated with RNA-pol 2. Also, we show using ChIP analyses that this interaction recruits BPTF to gene bodies. Next, we investigated functional consequences for NURF recruitment to gene bodies using Cyclin D1 (Ccnd1) gene as a model. These analyses revealed that NURF is required for normal mRNA processing and loss of NURF induces intron retention, which results in unstable transcripts. Finally, we show that the defect in mRNA processing is not specific to the Ccnd1 gene, as we observe similar defects in four other BPTF dependent genes. Together, our work uncovered new role of mammalian NURF complex in regulating gene expression through mRNA processing

Georgian Terrains: The Islamic Orient in James Elroy Flecker's Hassan

This study is mainly concerned with Georgian representations of the Islamic Orient. In placing it in a Saidian framework, it investigates how James Elroy Flecker’s Hassan treats the Islamic motifs. This play constructs the Orient as a network of destructive sexuality, alluring wealth, astonishing superstitions, and wonder in the exotic and the unfamiliar. Islam is blackly pictured, the Quran is undermined and the Abbasid Caliphate is severely attacked as an authority of corruption. By way of concluding, it can be suggested that Flecker’s misrepresentations of the Islamic Orient are attributed to his ideological hatred of Islam.Keywords: Orientalism; Georgian Poetry; The Arabian Nights; Abbasid Caliphate; the Bible; Islam, Christianity; Baghdad; Schizophrenic; Sufism; Lasciviousness; Houris; Islamic Sexuality; Oriental promiscuity; Femme fatale; Exoticism; Oriental superstition; Cruelty; Aggression; Violenc

Combined effect of frustration and dimerization in ferrimagnetic chains and square lattice

Within the zero-temperature linear spin-wave theory we have investigated the effect of frustration and dimerization of a Heisenberg system with alternating spins $s_{1}$ and $s_{2}$ on one- and two-dimensional lattices. The combined effect most visibly appears in the elementary excitation spectra. In contrast to the ground state energy that decreases with dimerization and increases with frustration, the excitation energies are shown to be suppressed in energy by both dimerization and frustration. The threshold value of frustration that signals a transition from a classical ferrimagnetic state to a spiral state, decreases with dimerization, showing that dimerization further helps in the phase transition. The correlation length and sublattice magnetization decrease with both dimerization and frustration indicating the destruction of the long-range classical ferrimagnetic. The linear spin wave theory shows that in the case of a square lattice, dimerization initially opposes the frustration-led transition to a spiral magnetic state, but then higher magnitudes of lattice deformation facilitate the transition. It also shows that the transition to spiral state is inhibited in a square lattice beyond a certain value of dimerization.Comment: 8 pages, latex, 12 postscript figure

Impact of chronic war trauma exposure on PTSD diagnosis from 2006 -2021: a longitudinal study in Palestine

© Crown 2023. This article is licensed under a Creative Commons Attribution 4.0. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Background: Trauma and Post-Traumatic Stress Disorders (PTSD) are a common result of the occurrence of natural and man-made disasters, the refugees are one of these traumas in which some resulted from political conflicts. Today, Palestinians are the largest refugee population in the world after the Nakba in 1948. This study aims to discover the impact of chronic war traumas on PTSD diagnosis and symptoms from 2006 to 2021. The current study is a follow-up of 607 adolescents and adults, aged between 10–30 years of which are also those children who participated in the 2006 study. This research used an adapted PTSD scale (PTSD-SRII, 33 items) from a validated original questionnaire developed by Altawil in 2008 to measure PTSD based on symptoms reported in DSM-IV and ICD-10. Results: The main findings show at least 97.2% of participants had experienced at least 6 traumatic experiences up to the year 2006 and 100% of the participants were exposed to traumas in 2021. This study found four categories of responses to chronic war trauma: (a) Acute group (n = 321, 52.9%) shows high PTSD symptoms in 2006 and in 2021; (b) Remitters group (n = 54, 8.9%) shows high PTSD symptoms in 2006 but did not show significant PTSD symptoms in 2021; (c) Delayed group (n = 204, 33.6%) shows no PTSD symptoms in 2006 but showed significant PTSD symptoms in 2021; (d) Resilient group (n = 28, 4.6%) had no PTSD symptoms in 2006 and did not show PTSD symptoms in 2021. Conclusions: The authors conclude that Palestinian children, youth, adults and their families in the Gaza Strip are continually at a huge risk of developing PTSD and other psychological problems. This means that Palestinians will suffer for a long time from PTSD, which should be called Chronic Traumatic Stress Disorder (CTSD) rather than PTSD. This PTSD or CTSD cannot be changed unless the root of the problem is solved by ending the 74 years of living under occupation.Peer reviewe

IMPACT OF ALCOHOL ON PANCREAS IN ALCOHOLIC PATIENTS

Objective: Prolonged hazardous drinking can result in progressive and irreversible damage to the pancreas gland. This occurs on the background of pancreatic inflammation, acinar atrophy and, ultimately, fibrosis and can result in significant exocrine and endocrine insufficiency. Withdrawal of alcohol at an early stage may arrest the process and, even when the condition is established, may reduce the number of inflammatory episodes and allow for better control of both exocrine and endocrine insufficiencies. This study is aimed to identify the impact of alcohol on the pancreas and to educate the patient about the importance of alcohol cessation. Methods: A retrospective study was conducted in a multispecialty clinic in the gastroenterology department for a period of one month. 60 patients were involved in the study who were diagnosed with pancreatitis (acute or chronic). The data was obtained by directly communicating with the patients which consisted of demographics and social habits. Results: Among 60 patients, the male was dominant (85%) than female (15%). Majority of the patients were in the age group of 41-60 y (65%) followed by patients 2140 y of age (25%) and patients above 60 y of age (10%). Epigastric pain was the most common presenting complaint in all patients (67%) followed by vomiting (33%). Majority of the patients involved are alcoholics (60%). Conclusion: Proper attention to the health at the time of diagnosis of disease by alcohol cessation can prevent the progression of the disease and helps the patient to lead a healthy life

Why do oligodendrocyte lineage cells express glutamate receptors?

The function of glutamate receptors on oligodendrocytes and their precursor cells is poorly understood, with their only clear action being to damage these cells in pathological conditions. Here we review recent studies of glutamate signalling to oligodendrocyte lineage cells, and explore what its physiological function may be