Genetic variability studies in amaranthus (Amaranthus spp.)

An investigation was carried out to estimate the nature and extent of genetic variability​ ​among twenty Amaranthus genotypes (Amaranthus spp.) under a randomized block designduring the year 2019-20. The phenotypic coefficient of variation was higher than the genotypic​ ​coefficient of variation for all the traits. High genotypic coefficient of variation (GCV) and​ ​phenotypic coefficient variation (PCV) was observed for leaf area, leaf area index, leaf area​ ​duration, AGR, dry weight of leaf per plant, specific leaf weight, speed of germination,​ ​chlorophyll content and ascorbic acid (vitamin C). Moderate GCV and PCV were observed​ ​for leaf length, leaf width, petiole length, dry weight of stem per plant, leaf: stem ratio and​ ​foliage yield per plant. The high estimates of heritability coupled with higher values of genetic​ ​advance as per cent mean (GAM) were observed for the parameters like test weight, speed of​ ​germination, germination percentage, seedling dry matter, seedling vigour index-1, seedling​ ​vigour index-2, plant height, leaf length, leaf width, leaf area, leaf area index, leaf area​ ​duration, AGR, specific leaf weight, stem weight per plot, dry weight of leaf, dry weight of​ ​stem, leaf: stem ratio, foliage yield per plant, chlorophyll, ascorbic acid and beta- carotene​ ​content which indicates the predominance of additive gene action. Arka Arunima,​ ​Chikmagalur local, IC-551486, IC-551494 and IC-551466 recorded high foliage yield per​ ​plot and these can be utilized in further breeding programmes

Proceedings of the Second workshop on scientific results of FORV Sagar Sampada

Since inception in 1984, the Fisheries and Oceanographic Research Vessel Sagar Sampada, with sophisticated modern facilities onboard, has undertaken more than 140 cruises all over the Indian Ocean primarily in our Exclusive Economic Zone for fishery and oceanographic survey. Scientists from many institutions have made use of this facility to explore the resource potential in our sea and to understand its correlation with the environmental parameters. These survey and exploration efforts are continuing. This volume contains many papers which are the outcome of research work carried out onboard 'Sagar Sampada' during last five years (1989-1993) and presented in a Workshop to evaluate the scientific work. About 60 papers, included in this volume, bring out the results pertaining to environmental assessment, hydrology, productivity estimates, fishery resources availability, fishing technology, pollution monitoring etc. These relate mainly to the Arabian Sea, Bay of Bengal and Lakshadweep, Andaman and Nicobar Islands groups in the EEZ of India. These efforts along with other mission-oriented cruises have yielded valuable information on the fishery resources and their distribution in space and time

Gaps and opportunities in refractory status epilepticus research in children: A multi-center approach by the Pediatric Status Epilepticus Research Group (pSERG)

PURPOSE: Status epilepticus (SE) is a life-threatening condition that can be refractory to initial treatment. Randomized controlled studies to guide treatment choices, especially beyond first-line drugs, are not available. This report summarizes the evidence that guides the management of refractory convulsive SE (RCSE) in children, defines gaps in our clinical knowledge and describes the development and works of the \u27pediatric Status Epilepticus Research Group\u27 (pSERG). METHODS: A literature review was performed to evaluate current gaps in the pediatric SE and RCSE literature. In person and online meetings helped to develop and expand the pSERG network. RESULTS: The care of pediatric RCSE is largely based on extrapolations of limited evidence derived from adult literature and supplemented with case reports and case series in children. No comparative effectiveness trials have been performed in the pediatric population. Gaps in knowledge include risk factors for SE, biomarkers of SE and RCSE, second- and third-line treatment options, and long-term outcome. CONCLUSION: The care of children with RCSE is based on limited evidence. In order to address these knowledge gaps, the multicenter pSERG was established to facilitate prospective collection, analysis, and sharing of de-identified data and biological specimens from children with RCSE. These data will allow identification of treatment strategies associated with better outcomes and delineate evidence-based interventions to improve the care of children with SE

SLC35A2â CDG: Functional characterization, expanded molecular, clinical, and biochemical phenotypes of 30 unreported Individuals

Pathogenic de novo variants in the Xâ linked gene SLC35A2 encoding the major Golgiâ localized UDPâ galactose transporter required for proper protein and lipid glycosylation cause a rare type of congenital disorder of glycosylation known as SLC35A2â congenital disorders of glycosylation (CDG; formerly CDGâ IIm). To date, 29 unique de novo variants from 32 unrelated individuals have been described in the literature. The majority of affected individuals are primarily characterized by varying degrees of neurological impairments with or without skeletal abnormalities. Surprisingly, most affected individuals do not show abnormalities in serum transferrin Nâ glycosylation, a common biomarker for most types of CDG. Here we present data characterizing 30 individuals and add 26 new variants, the single largest study involving SLC35A2â CDG. The great majority of these individuals had normal transferrin glycosylation. In addition, expanding the molecular and clinical spectrum of this rare disorder, we developed a robust and reliable biochemical assay to assess SLC35A2â dependent UDPâ galactose transport activity in primary fibroblasts. Finally, we show that transport activity is directly correlated to the ratio of wildâ type to mutant alleles in fibroblasts from affected individuals.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/150498/1/humu23731_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150498/2/humu23731-sup-0001-Supp_Mat__2019.2.10_.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/150498/3/humu23731.pd

Isolation, Characterization, Cultivation of Trichoderma Species and its Applications as Biopesticide

Trichoderma is a genus of anamorphic fungi that are mostly found in rhizospere soil. and can be used to combat fungal phytopathogens (Fusarium spp which cause the wilt stem in chilli plant). Trichoderma species have long been employed as a biopesticide in agriculture. It also provides the plant with growth-promoting chemicals, such as Indole acetic acid and ammonia. The molecular mechanisms underlying this very desirable favourable effect of plant growth promotion, which includes improved nutrient availability and uptake for the plant, are not entirely understood. Extracellular enzyme activity of cellulase, lipase, luccase, protease, and amylase in Trichoderma spp. In vitro antagonistic potential of Trichoderma spp. against Fusarium spp. was investigated. Keywords: Trichoderma, Pytopathogens, Biopesticide, Fusariu

Surgical Treatment of Pediatric Epileptic Encephalopathies

Pediatric epileptiform encephalopathies are a group of neurologically devastating disorders related to uncontrolled ictal and interictal epileptic activity, with a poor prognosis. Despite the number of pharmacological options for treatment of epilepsy, many of these patients are drug resistant. For these patients with uncontrolled epilepsy, motor and/or neuropsychological deterioration is common. To prevent these secondary consequences, surgery is often considered as either a curative or a palliative option. Magnetic resonance imaging to look for epileptic lesions that may be surgically treated is an essential part of the workup for these patients. Many surgical procedures for the treatment of epileptiform encephalopathies have been reported in the literature. In this paper the evidence for these procedures for the treatment of pediatric epileptiform encephalopathies is reviewed

Proceedings of the Second Workshop on Scientific Results of FORV Sugar Sampudu Planktonic amphipod distribution in the deep scattering layer of the Exclusive Economic Zone of India

ABSTRACT The amphipods collected from the deep scattering layer (DSL) of Arabian Sea and Bay of Bengal belonged to 13 families viz. Cystisomatidae, Oxyccphalidae, Pronoidae, Anapronoidae, Vibiliidae, Lycaeidae, Lycaeopsidae, Phronimidae, Platyscelidae, Phrosinidae, Scinidae, Lanceolidae and Hyperiidae. The amphipod abundance showed wide fluctuations. Oxyccphalidae, Phronimidae and platyscelidae were widely distributed in both the shelf and oceanic regions of the west coast. In the east coast Phronimidae and Phrosinidae were widely distributed in the shelf and these two families together with Vibiliidae and Platyscelidae were widely distributed in the oceanic regions. In this investigation only Phronimidae was found to have wide distribution in the shelf and oceanic regions of both the Arabian Sea and Bay of Bengal. Vibiliidae and Phrosinidae showed aggregation in the oceanic region and these two together with Lycaeidae showed such a trend in the shelf of the west coast. Similarly in the east coast while Lycaeidae and Pronoidae showed aggregation in the shelf, only the former showed that trend in the oceanic region. While Cystisomatidae had a moderate distribution in the shelf of both the coasts and in the oceanic region of the west coast, it had a poor distribution in the oceanic region of the east coast. Vibiliidae and Oxycephalidae were moderately distributed in both the shelf and oceanic regions of the east coast. AH other families of amphipods had a poor distribution

Proceedings of the Second Workshop on Scientific Results of FORV Sugar Sampudu Some observations on primary production and plankton biomass along the continental shelf and slope off the northeast coast of India during January 1989

ABSTRACT The present study deals with the quantitative aspects of chlorophyll pigments, primary productivity and plankton biomass from the continental shelf and slope off the northeast coast of India between 16° and 20°N latitudes towards the end of northeast monsoon season. In surface waters, the mean values of chl-a, -b, and -c were 0.249,0.275 and 0.837 mg/m^ along the shelf and 0.246,0.260 and 0.805 mg/m^ in the slope respectively while the net primary productivity values were 0.074 and 0.081 g C/m /d for the shelf and slope waters respectively. Column productivity in the upper 0-50 m water in the shelf and slope regions were 2.9 and 3.25 g C/m /d with an average production of 3.08 g C/m /d. Higher rate of production was observed around 18° and 20°N latitudes. Zooplankton biomass exhibited progressive increase in volume from 16° to 20°N. The estimated mean zooplankton biomass volume of the study area was 28.83 ml/m . The mean transfer coefficient from primary to secondary production was found to be 14% when 50% of the zooplankton biomass was considered as the daily rate of production. From the mean primary and secondary productivity values, potential tertiary production of pelagic fishery resources in the upper 0-50 m water column of the study area for the month was assessed. The factors that are likely to cause error in the estimation of secondary production are discussed

The effect of emergency department crowding on clinically oriented outcomes

BACKGROUND: An Institute of Medicine (IOM) report defines six domains of quality of care: safety, patient-centeredness, timeliness, efficiency, effectiveness, and equity. The effect of emergency department (ED) crowding on these domains of quality has not been comprehensively evaluated. OBJECTIVES: The objective was to review the medical literature addressing the effects of ED crowding on clinically oriented outcomes (COOs). METHODS: We reviewed the English-language literature for the years 1989-2007 for case series, cohort studies, and clinical trials addressing crowding\u27s effects on COOs. Keywords searched included ED crowding, ED overcrowding, mortality, time to treatment, patient satisfaction, quality of care, and others. RESULTS: A total of 369 articles were identified, of which 41 were kept for inclusion. Study quality was modest; most articles reflected observational work performed at a single institution. There were no randomized controlled trials. ED crowding is associated with an increased risk of in-hospital mortality, longer times to treatment for patients with pneumonia or acute pain, and a higher probability of leaving the ED against medical advice or without being seen. Crowding is not associated with delays in reperfusion for patients with ST-elevation myocardial infarction. Insufficient data were available to draw conclusions on crowding\u27s effects on patient satisfaction and other quality endpoints. CONCLUSIONS: A growing body of data suggests that ED crowding is associated both with objective clinical endpoints, such as mortality, as well as clinically important processes of care, such as time to treatment for patients with time-sensitive conditions such as pneumonia. At least two domains of quality of care, safety and timeliness, are compromised by ED crowding