Bifidobacterium animalis subsp lactis HN019 presents antimicrobial potential against periodontopathogens and modulates the immunological response of oral mucosa in periodontitis patients

Objective To evaluate the effects of Bifidobacterium animalis subsp. lactis HN019 (HN019) on clinical periodontal parameters (plaque accumulation and gingival bleeding), on immunocompetence of gingival tissues [expression of beta-defensin (BD)-3, toll-like receptor 4 (TLR4), cluster of differentiation(CD)-57 and CD-4], and on immunological properties of saliva (IgA levels) in non-surgical periodontal therapy in generalized chronic periodontitis (GCP) patients. Adhesion to buccal epithelial cells (BEC) and the antimicrobial properties of HN019 were also investigated. Materials and methods Thirty patients were recruited and monitored clinically at baseline (before scaling and root planing-SRP) and after 30 and 90 days. Patients were randomly assigned to Test (SRP +Probiotic, n = 15) or Control (SRP+Placebo, n = 15) group. Probiotic lozenges were used for 30 days. Gingival tissues and saliva were immunologically analyzed. The adhesion of HN019 with or without Porphyromonas gingivalis in BEC and its antimicrobial properties were investigated in in vitro assays. Data were statistically analyzed (p<0.05). Results Test group presented lower plaque index (30 days) and lower marginal gingival bleeding (90 days) when compared with Control group. Higher BD-3, TLR4 and CD-4 expressions were observed in gingival tissues in Test group than in Control group. HN019 reduced the adhesion of P. gingivalis to BEC and showed antimicrobial potential against periodontopathogens. Conclusion Immunological and antimicrobial properties of B. lactis HN019 make it a potential probiotic to be used in non-surgical periodontal therapy of patients with GCP.Fil: Invernici, Marcos M.. Universidade de Sao Paulo; BrasilFil: Furlaneto, Flávia A. C.. Universidade de Sao Paulo; BrasilFil: Salvador, Sérgio L. Universidade de Sao Paulo; BrasilFil: Ouwehand, Arthur C.. Dupont, Nutrition and Health; FinlandiaFil: Salminen, Seppo. University of Turku. Functional Foods Forum; FinlandiaFil: Mantziari, Anastasia. University of Turku. Functional Foods Forum; FinlandiaFil: Vinderola, Celso Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Ervolino, Edilson. São Paulo State University. Division of Histology, Department of Basic Sciences, Dental School of Araçatuba; BrasilFil: Santana, Sandro Isaías. Universidade de Sao Paulo; BrasilFil: Silva, Pedro Henrique Felix. Universidade de Sao Paulo; BrasilFil: Messora, Michel R.. Universidade de Sao Paulo; Brasi

MAMMALS IN PORTUGAL : A data set of terrestrial, volant, and marine mammal occurrences in P ortugal

Mammals are threatened worldwide, with 26% of all species being includedin the IUCN threatened categories. This overall pattern is primarily associatedwith habitat loss or degradation, and human persecution for terrestrial mam-mals, and pollution, open net fishing, climate change, and prey depletion formarine mammals. Mammals play a key role in maintaining ecosystems func-tionality and resilience, and therefore information on their distribution is cru-cial to delineate and support conservation actions. MAMMALS INPORTUGAL is a publicly available data set compiling unpublishedgeoreferenced occurrence records of 92 terrestrial, volant, and marine mam-mals in mainland Portugal and archipelagos of the Azores and Madeira thatincludes 105,026 data entries between 1873 and 2021 (72% of the data occur-ring in 2000 and 2021). The methods used to collect the data were: live obser-vations/captures (43%), sign surveys (35%), camera trapping (16%),bioacoustics surveys (4%) and radiotracking, and inquiries that represent lessthan 1% of the records. The data set includes 13 types of records: (1) burrowsjsoil moundsjtunnel, (2) capture, (3) colony, (4) dead animaljhairjskullsjjaws, (5) genetic confirmation, (6) inquiries, (7) observation of live animal (8),observation in shelters, (9) photo trappingjvideo, (10) predators dietjpelletsjpine cones/nuts, (11) scatjtrackjditch, (12) telemetry and (13) vocalizationjecholocation. The spatial uncertainty of most records ranges between 0 and100 m (76%). Rodentia (n=31,573) has the highest number of records followedby Chiroptera (n=18,857), Carnivora (n=18,594), Lagomorpha (n=17,496),Cetartiodactyla (n=11,568) and Eulipotyphla (n=7008). The data setincludes records of species classified by the IUCN as threatened(e.g.,Oryctolagus cuniculus[n=12,159],Monachus monachus[n=1,512],andLynx pardinus[n=197]). We believe that this data set may stimulate thepublication of other European countries data sets that would certainly contrib-ute to ecology and conservation-related research, and therefore assisting onthe development of more accurate and tailored conservation managementstrategies for each species. There are no copyright restrictions; please cite thisdata paper when the data are used in publications.info:eu-repo/semantics/publishedVersio

Self-similar expansion of a turbulent Bose-Einstein condensate: a generalized hydrodynamic model

We generalize the superfluid hydrodynamic equations to describe the expansion of a turbulent condensate cloud. Our Lagrangian formalism includes the kinetic energy associated with an entangled vortex configuration. The expressions developed here clarify the physics behind the self-similar free expansion as seen in experiments. This feature is a crucial and characteristic signature of turbulence. Our present study improves on our previous model (Caracanhas et al. in J. Low Temp. Phys. 166: 49, 2012).FAPESP (CEPID)CNPq / CAPES (INCT)NSF (PHY09-69790, PHY10-1066292

Consumo de combustível na operação de subsolagem realizada antes e depois de diferentes sistemas de preparo periódico do solo Fuel consumption in the subsoiling operation accomplished before and after different preparation systems of periodic soil tillage

A subsolagem tem aumentado nos últimos anos de forma indiscriminada, faltando estudos que norteiem os melhores procedimentos para que novos problemas não sejam acrescentados devido a subsolagens inadequadas ou mesmo em solos onde a operação é desnecessária, e principalmente buscar redução no consumo de combustível. Dessa forma, este trabalho teve o objetivo de avaliar o consumo de combustível na operação de subsolagem efetuada antes e depois de diferentes sistemas de preparo periódico do solo, classificado como Nitossolo Vermelho distroférrico. Os preparos foram realizados com arado de discos, arado de discos mais uma gradagem de nivelamento, grade pesada, grade pesada mais gradagem de nivelamento e escarificador. A realização da subsolagem depois dos sistemas de preparo periódico requereu 15% menos de potência na barra de tração. A subsolagem depois dos diferentes sistemas de preparo economizou 16,5% de combustível por área. O deslizamento das rodas motrizes e a velocidade média operacional obtiveram melhor desempenho quando se realizou a subsolagem depois do preparo do solo.<br>The subsoiling has had a great increase in the last few years, but there is still a lack of studies that guide the best procedures so that new problems do not appear because of inadequate subsoiling or even in soil where the same operation is unnecessary, and primarily look for a reduction in the consumption of fuel. Thus, this work had the objective of evaluating the fuel consumption in the operation of subsoiling performed before and after different systems of preparation of a regular land classified as Nitossolo Red Distroférrico. The periodic tillage systems were: plowing with disks, plowing with disks followed by one leveling, plowing harrow, plowing harrow followed by leveling and chiseling. The accomplishment of the subsoiling after the preparation of periodic systems required 15% less power in the bar of traction. The subsoiling after the different systems of preparation saved 16.5% of fuel per area. The slip of the driving wheels and average operational speed obtained better performance when it took the subsoiling after preparing the soil

Effects of the probiotic Bifidobacterium animalis subsp. lactis on the non-surgical treatment of periodontitis. A histomorphometric, microtomographic and immunohistochemical study in rats.

Lactobacillus probiotics have been investigated in periodontitis. However, the effects of the genus Bifidobacterium on periodontitis are hardly known. This study evaluated the effects of the probiotic (PROB) Bifidobacterium animalis subsp. lactis (B. lactis) HN019 as an adjunct to scaling and root planing (SRP) in rats with experimental periodontitis (EP). At baseline, 32 rats were assigned to 4 groups: C (control), PROB, EP-SRP and EP-SRP-PROB. In groups EP-SRP and EP-SRP-PROB, the mandibular first molars of the animals received a ligature. At day 14, the ligatures were removed and SRP was performed. Animals of groups PROB and EP-SRP-PROB were orally administered with 10 mL/day of 109 colony forming units of B. lactis HN019 for 15 days, starting at day 14. Animals were euthanized at day 29. Histomorphometric, microtomographic and immunohistochemical analyses were performed. Microbiological effects of B. lactis on biofilm were also evaluated. Data were statistically analyzed (ANOVA, Tukey; Kruskal-Wallis, Dunn's; Two-tailed t-test; p<0.05). Group EP-SRP-PROB presented reduced alveolar bone resorption and attachment loss when compared with Group EP-SRP (p<0.05). Group EP-SRP-PROB showed significantly fewer osteoclasts, increased expression of anti-inflammatory cytokines and reduced expression of proinflammatory cytokines compared with Group EP-SRP (p<0.05). B. lactis promoted a higher ratio between aerobic and anaerobic bacteria in biofilm samples (p<0.05). B. lactis HN019 may have a role in the treatment of EP in rats, as an adjunct to SRP

Immunohistochemical analyses—IL-1β and CINC.

<p>Medians, interquartile range and maximum and minimum values of the immunolabeling scores for IL-1β (A) and CINC (B), with comparisons among groups. Photomicrographs showing immunolabeling for IL-1β (C-F) and CINC (G-J) in the furcation regions of mandibular first molars: Group C (C and G); Group PROB (D and H); Group EP-SRP (E and I); Group EP-SRP-PROB (F and J). Abbreviations: ab = alveolar bone; ct = connective tissue; pdl = periodontal ligament; * = <i>p</i><0.05. Scale bars: C-J = 80 μm. (Hematoxylin counterstaining).</p

Histomorphometric analysis of periodontal tissues.

<p>Means and standard deviations of ANB (A; furcation area) and AL (B; interproximal area), with comparisons among groups. Photomicrographs of periodontal tissues in the furcation (C-F) and interproximal areas (G-J) of mandibular first molars: Group C (C and G); Group PROB (D and H); Group EP-SRP (E and I); Group EP-SRP-PROB (F and J). Abbreviations and symbols: ab = alveolar bone; ct = connective tissue; pdl = periodontal ligament; ANB = area of no bone; AL = attachment loss; FM = first molar; SM = second molar; black arrows = cementoenamel junction; white arrows = epithelial attachment; * = <i>p</i><0.05; ** = <i>p</i><0.01; *** = <i>p</i><0.001. Scale bars: C-J = 200 μm. (Hematoxylin and Eosin stain).</p