Comparative study of green sub- and supercritical processes to obtain carnosic acid and carnosol-enriched rosemary extracts with in vitro anti-proliferative sctivity on colon cancer cells

In the present work, four green processes have been compared to evaluate their potential to obtain rosemary extracts with in vitro anti-proliferative activity against two colon cancer cell lines (HT-29 and HCT116). The processes, carried out under optimal conditions, were: (1) pressurized liquid extraction (PLE, using an hydroalcoholic mixture as solvent) at lab-scale; (2) Single-step supercritical fluid extraction (SFE) at pilot scale; (3) Intensified two-step sequential SFE at pilot scale; (4) Integrated PLE plus supercritical antisolvent fractionation (SAF) at pilot scale. Although higher extraction yields were achieved by using PLE (38.46% dry weight), this extract provided the lowest anti-proliferative activity with no observed cytotoxic effects at the assayed concentrations. On the other hand, extracts obtained using the PLE + SAF process provided the most active rosemary extracts against both colon cancer cell lines, with LC50 ranging from 11.2 to 12.4 µg/mL and from 21.8 to 31.9 µg/mL for HCT116 and HT-29, respectively. In general, active rosemary extracts were characterized by containing carnosic acid (CA) and carnosol (CS) at concentrations above 263.7 and 33.9 mg/g extract, respectively. Some distinct compounds have been identified in the SAF extracts (rosmaridiphenol and safficinolide), suggesting their possible role as additional contributors to the observed strong anti-proliferative activity of CA and CS in SAF extracts.This work was supported by the projects AGL2014-53609-P (Ministerio de Economía y Competitividad, Spain) and S2013/ABI-2728 (Comunidad de Madrid).We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI)

Cytomolecular identification of individual wheat-wheat chromosome arm associations in wheat-rye hybrids

Chromosome pairing in the meiotic metaphase I of wheatrye hybrids has been characterized by sequential genomic and fluorescent in situ hybridization allowing not only the discrimination of wheat and rye chromosomes, but also the identification of the individual wheat and rye chromosome arms involved in the chromosome associations. The majority of associations (93.8%) were observed between the wheat chromosomes. The largest number of wheat-wheat chromosome associations (53%) was detected between the A and D genomes, while the frequency of B-D and A-B associations was significantly lower (32 and 8%, respectively). Among the A-D chromosome associations, pairing between the 3AL and 3DL arms was observed with the highest frequency, while the most frequent of all the chromosome associations (0.113/ cell) was found to be the 3DS-3BS. Differences in the pairing frequency of the individual chromosome arms of wheat-rye hybrids have been discussed in relation to the homoeologous relationships between the constituent genomes of hexaploid wheat

Motion of CMS detector and mechanical structures during Magnet Cycles and Stability Periods from 2008 to 2013 as observed by the Link Alignment System

Magnet Cycles and Stability Periods of the CMS Experiment are studied with the Alignment Link System data recorded along the 2008-2013 years of operation. The motions of the mechanical structures due to the magnetic field forces are studied and the mechanical stability of the detector during the physics data taking periods is verified.Peer Reviewe

Recovering Bioactive Compounds from Olive Oil Filter Cake by Advanced Extraction Techniques

The potential of by-products generated during extra-virgin olive oil (EVOO) filtration as a natural source of phenolic compounds (with demonstrated bioactivity) has been evaluated using pressurized liquid extraction (PLE) and considering mixtures of two GRAS (generally recognized as safe) solvents (ethanol and water) at temperatures ranging from 40 to 175 °C. The extracts were characterized by high-performance liquid chromatography (HPLC) coupled to diode array detection (DAD) and electrospray time-of-flight mass spectrometry (HPLC-DAD-ESI-TOF/MS) to determine the phenolic-composition of the filter cake. The best isolation procedure to extract the phenolic fraction from the filter cake was accomplished using ethanol and water (50:50, v/v) at 120 °C. The main phenolic compounds identified in the samples were characterized as phenolic alcohols or derivatives (hydroxytyrosol and its oxidation product), secoiridoids (decarboxymethylated and hydroxylated forms of oleuropein and ligstroside aglycones), flavones (luteolin and apigenin) and elenolic acid derivatives. The PLE extraction process can be applied to produce enriched extracts with applications as bioactive food ingredients, as well as nutraceuticals

Amaranth: distribution and morphological diversity of the genetic resource in parts of the Mayan region (southeast of Mexico, Guatemala and Honduras)

Antecedentes y Objetivos: El amaranto es de interés mundial por su valor nutricional como verdura y grano. Las especies cultivadas de mayor importancia comercial por su producción de grano son Amaranthus caudatus, A. cruentus y A. hypochondriacus. Dos hipótesis aceptadas sobre el origen de A. cruentus consideran a A. hybridus como su ancestro. Los objetivos del presente estudio son 1) reportar la distribución actualizada y frecuencia de A. cruentus y A. hybridus en el sureste de México, Guatemala y Honduras y 2) evaluar su diversidad morfológica mediante caracteres florales y del fruto de ambas especies en México y Guatemala.Métodos: La información proveniente de 147 ejemplares de herbario fue empleada para actualizar la distribución y frecuencia de A. cruentus y A. hybridus en la zona de estudio, posible área de domesticación. La diversidad a nivel morfológico se determinó del análisis de 22 caracteres relacionados a estructuras de flores pistiladas y del fruto, usando un análisis multivariado de componentes principales y evaluando para ello 15 ejemplares de A. cruentus y 31 de A. hybridus procedentes de Guatemala y el sureste de México.Resultados clave: La mayor distribución de A. cruentus se presentó en Guatemala y, en el caso de A. hybridus en México. La variación acumulada en los tres primeros componentes principales fue de 58% para ambas especies. La variación morfológica para las dos especies se asoció principalmente con caracteres relacionados a los sépalos internos y externos. El gráfico del análisis de componentes principales permite observar una amplia variación morfológica en A. cruentus y A. hybridus, independiente de su distribución geográfica en ambos casos.Conclusiones: La distribución geográfica de A. cruentus en México es más amplia con respecto a reportes previos. Se describe la diversidad morfológica de estructuras de flores pistiladas y frutos en A. cruentus y A. hybridus.Background and Aims: Amaranth is of worldwide interest for its nutritional value as a vegetable and grain. The principal cultivated species used as grain are Amaranthus caudatus, A. cruentus and A. hypochondriacus. Two accepted hypotheses about the origin of A. cruentus consider A. hybridus as its ancestor. The objectives of this study are 1) to report the updated distribution and frequency of A. cruentus and A. hybridus in southeastern Mexico, Guatemala, and Honduras and 2) to assess their morphological diversity, using floral and fruit characters of both species in Mexico and Guatemala.Methods: The information gathered from 147 herbarium examples was used to update the distribution and determine the frequency of A. cruentus and A. hybridus in the study area, possible area of domestication. Morphological diversity was determined using 22 morphological characters related to pistillate flowers and fruits by conducting a multivariate analysis of main components in a sampling of 15 specimens of A. cruentus and 31 of A. hybridus from Guatemala and southeastern Mexico.Key results: The main distribution of A. cruentus was shown in Guatemala, and of A. hybridus in Mexico. The accumulated variation in the first three principal components was 58% for both species. The morphological variation for both species was mainly associated with characters related to the internal and external sepals. The principal components analysis graph showed wide morphological variation in A. cruentus and A. hybridus regardless of their geographical distribution in both cases.Conclusions: The geographical distribution of A. cruentus in Mexico is wider than previously reported. Diversity was described using morphological characters of pistillate flowers and fruits for A. cruentus and A. hybridus

Measurement of four-jet production in proton-proton collisions at √s=7 TeV

This article is available under the terms of the Creative Commons Attribution 3.0 License.-- et al.Measurements of the differential cross sections for the production of exactly four jets in proton-proton collisions are presented as a function of the transverse momentum pT and pseudorapidity η, together with the correlations in azimuthal angle and the pT balance among the jets. The data sample was collected in 2010 at a center-of-mass energy of 7 TeV with the CMS detector at the LHC, with an integrated luminosity of 36pb-1. The cross section for exactly four jets, with two hard jets of pT>50GeV each, together with two jets of pT>20GeV each, within |η|<4.7 is measured to be σ= 330±5(stat.)±45(syst.)nb. It is found that fixed-order matrix element calculations including parton showers describe the measured differential cross sections in some regions of phase space only, and that adding contributions from double parton scattering brings the Monte Carlo predictions closer to the data.We acknowledge support from BMWF and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MEYS (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES (Croatia); RPF (Cyprus); MoER, SF0690030s09 and ERDF (Estonia); Academy of Finland, MEC, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF and WCU (Korea); LAS (Lithuania); CINVESTAV, CONACYT, SEP, and UASLPFAI (Mexico); MSI (New Zealand); PAEC (Pakistan); MSHE and NSC (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine Uzbekistan); MON, RosAtom, RAS and RFBR (Russia); MSTD (Serbia); SEIDI and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); ThEP, IPST and NECTEC (Thailand); TUBITAK and TAEK (Turkey); NASU (Ukraine); STFC (United Kingdom); DOE and NSF (USA). Individuals have received support from the Marie-Curie programme and the European Research Council and EPLANET(EuropeanUnion); the Leventis Foundation; the A.P. Sloan Foundation; the Alexander von Humboldt Foundation; the Belgian Federal Science Policy Office; the Fonds pour la Formation à la Recherche dans l’Industrie et dansl’Agriculture (FRIA-Belgium); the Agentschap voor Innovatie door Wetenschapen Technologie (IWT-Belgium); the Ministry of Education, Youth and Sports(MEYS) of Czech Republic; the Council of Science and Industrial Research, India; the Compagnia di San Paolo(Torino); the HOMINGPLUS programme of Foundation for PolishScience, cofinanced by EU,Re-gional Development Fund; and the Thalis and Aristeia programmes cofinanced by EU-ESFand the Greek NSRF.Peer reviewe

Observation of long-range, near-side angular correlations in pPb collisions at the LHC

This article is published Open Access at sciencedirect.com. It is distributed under the terms of the Creative Commons Attribution License 3.0.-- et al.Results on two-particle angular correlations for charged particles emitted in pPb collisions at a nucleon–nucleon center-of-mass energy of 5.02 TeV are presented. The analysis uses two million collisions collected with the CMS detector at the LHC. The correlations are studied over a broad range of pseudorapidity, η , and full azimuth, ϕ , as a function of charged particle multiplicity and particle transverse momentum, pTpT. In high-multiplicity events, a long-range (2<|Δη|<42<|Δη|<4), near-side (Δϕ≈0Δϕ≈0) structure emerges in the two-particle Δη –Δϕ correlation functions. This is the first observation of such correlations in proton–nucleus collisions, resembling the ridge-like correlations seen in high-multiplicity pp collisions at s=7 TeV and in AA collisions over a broad range of center-of-mass energies. The correlation strength exhibits a pronounced maximum in the range of pT=1–1.5 GeV/cpT=1–1.5 GeV/c and an approximately linear increase with charged particle multiplicity for high-multiplicity events. These observations are qualitatively similar to those in pp collisions when selecting the same observed particle multiplicity, while the overall strength of the correlations is significantly larger in pPb collisions.Acknowledge support from BMWF and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MEYS (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES (Croatia); RPF (Cyprus); MoER, SF0690030s09 and ERDF (Estonia); Academy of Finland, MEC, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF and WCU (Korea); LAS (Lithuania); CINVESTAV, CONACYT, SEP, and UASLPFAI (Mexico); MSI (New Zealand); PAEC (Pakistan); MSHE and NSC (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine Uzbekistan); MON, RosAtom, RAS and RFBR (Russia); MSTD (Serbia); SEIDI and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); ThEP, IPST and NECTEC (Thailand); TUBITAK and TAEK (Turkey); NASU (Ukraine); STFC (United Kingdom); DOE and NSF (USA).Peer Reviewe

Measurement of the Υ(1S), Υ(2S), and Υ(3S) polarizations in pp collisions at √s=7 TeV

The polarizations of the υ(1S), υ(2S), and υ(3S) mesons are measured in proton-proton collisions at √s=7 TeV, using a data sample of υ(nS)→μ+μ- decays collected by the CMS experiment, corresponding to an integrated luminosity of 4.9 fb-1. The dimuon decay angular distributions are analyzed in three different polarization frames. The polarization parameters λ™, λφ, and λ, as well as the frame-invariant quantity λ, are presented as a function of the υ(nS) transverse momentum between 10 and 50 GeV, in the rapidity ranges |y|<0.6 and 0.6<|y|<1.2. No evidence of large transverse or longitudinal polarizations is seen in the explored kinematic region. © 2013 CERN.We acknowledge support from: BMWF and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES (Croatia); RPF (Cyprus); MoER, SF0690030s09 and ERDF (Estonia); Academy of Finland, MEC, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF and WCU (Korea); LAS (Lithuania); CINVESTAV, CONACYT, SEP, and UASLP-FAI (Mexico); MSI (New Zealand); PAEC (Pakistan); MSHE and NSC (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine, and Uzbekistan); MON, RosAtom, RAS, and RFBR (Russia); MSTD (Serbia); SEIDI and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); TUBITAK and TAEK (Turkey); STFC (United Kingdom); and DOE and NSF (USA).Peer Reviewe

Search for narrow resonances using the dijet mass spectrum in pp collisions at √s=8 TeV

Results are presented of a search for the production of new particles decaying to pairs of partons (quarks, antiquarks, or gluons), in the dijet mass spectrum in proton-proton collisions at √s=8 TeV. The data sample corresponds to an integrated luminosity of 4.0 fb-1, collected with the CMS detector at the LHC in 2012. No significant evidence for narrow resonance production is observed. Upper limits are set at the 95% confidence level on the production cross section of hypothetical new particles decaying to quark-quark, quark-gluon, or gluon-gluon final states. These limits are then translated into lower limits on the masses of new resonances in specific scenarios of physics beyond the standard model. The limits reach up to 4.8 TeV, depending on the model, and extend previous exclusions from similar searches performed at lower collision energies. For the first time mass limits are set for the Randall-Sundrum graviton model in the dijet channel.We acknowledge support from BMWF and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MEYS (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES (Croatia); RPF (Cyprus); MoER, SF0690030s09 and ERDF (Estonia); Academy of Finland, MEC, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NKTH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); NRF and WCU (Korea); LAS (Lithuania); CINVESTAV, CONACYT, SEP, and UASLPFAI (Mexico); MSI (New Zealand); PAEC (Pakistan); MSHE and NSC (Poland); FCT (Portugal); JINR (Armenia, Belarus, Georgia, Ukraine Uzbekistan); MON, RosAtom, RAS and RFBR (Russia); MSTD (Serbia); SEIDI and CPAN (Spain); Swiss Funding Agencies (Switzerland); NSC (Taipei); ThEP, IPST and NECTEC (Thailand); TUBITAK and TAEK (Turkey); NASU (Ukraine); STFC (United Kingdom); DOE and NSF (USA).Peer Reviewe

Search for pair production of excited top quarks in the lepton + jets final state

Open Access: This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0).-- et al.A search is performed for pair-produced spin-3/2 excited top quarks (t*t̄*), each decaying to a top quark and a gluon. The search uses data collected with the CMS detector from pp collisions at a center-of-mass energy of √s = 8 TeV, selecting events that have a single isolated muon or electron, an imbalance in transverse momentum, and at least six jets, of which one must be compatible with originating from the fragmentation of a b quark. The data, corresponding to an integrated luminosity of 19.5 fb-1, show no significant excess over standard model predictions, and provide a lower limit of 803 GeV at 95% confidence on the mass of the spin-3/2 t* quark in an extension of the Randall-Sundrum model, assuming a 100% branching fraction of its decay into a top quark and a gluon. This is the first search for a spin-3/2 excited top quark performed at the LHC.We acknowledge the enduring support for the construction and operation of the LHC and the CMS detector provided by the following funding agencies: the Austrian Federal Ministry of Science and Research; the Belgian Fonds de la Recherche Scientifique, and Fonds voor Wetenschappelijk Onderzoek; the Brazilian Funding Agencies (CNPq, CAPES, FAPERJ, and FAPESP); the Bulgarian Ministry of Education, Youth and Science; CERN; the Chinese Academy of Sciences, Ministry of Science and Technology, and National Natural Science Foundation of China; the Colombian Funding Agency (COLCIENCIAS); the Croatian Ministry of Science, Education and Sport; the Research Promotion Foundation, Cyprus; the Ministry of Education and Research, Recurrent financing contract SF0690030s09 and European Regional Development Fund, Estonia; the Academy of Finland, Finnish Ministry of Education and Culture, and Helsinki Institute of Physics; the Institut National de Physique Nucle´aire et de Physique des Particules/CNRS, and Commissariat à l’Énergie Atomique et aux E'nergies Alternatives/CEA, France; the Bundesministerium für Bildung und Forschung, Deutsche Forschungsgemeinschaft, and Helmholtz-Gemeinschaft Deutscher Forschungszentren, Germany; the General Secretariat for Research and Technology, Greece; the National Scientific Research Foundation, and National Office for Research and Technology, Hungary; the Department of Atomic Energy and the Department of Science and Technology, India; the Institute for Studies in Theoretical Physics and Mathematics, Iran; the Science Foundation, Ireland; the Istituto Nazionale di Fisica Nucleare, Italy; the Korean Ministry of Education, Science and Technology and the World Class University program of NRF, Republic of Korea; the Lithuanian Academy of Sciences; the Mexican Funding Agencies (CINVESTAV, CONACYT, SEP, and UASLP-FAI); the Ministry of Science and Innovation, New Zealand; the Pakistan Atomic Energy Commission; the Ministry of Science and Higher Education and the National Science Centre, Poland; the Fundaçao para a Ciência e a Tecnologia, Portugal; JINR (Armenia, Belarus, Georgia, Ukraine, Uzbekistan); the Ministry of Education and Science of the Russian Federation, the Federal Agency of Atomic Energy of the Russian Federation, Russian Academy of Sciences, and the Russian Foundation for Basic Research; the Ministry of Science and Technological Development of Serbia; the Secretaría de Estado de Investigación, Desarrollo e Innovación and Programa Consolider-Ingenio 2010, Spain; the Swiss Funding Agencies (ETH Board, ETH Zurich, PSI, SNF, UniZH, Canton Zurich, and SER); the National Science Council, Taipei; the Thailand Center of Excellence in Physics, the Institute for the Promotion of Teaching Science and Technology of Thailand and the National Science and Technology Development Agency of Thailand; the Scientific and Technical Research Council of Turkey, and Turkish Atomic Energy Authority; the Science and Technology Facilities Council, U.K.; the U.S. Department of Energy, and the U.S. National Science Foundation. Individuals have received support from the Marie-Curie program and the European Research Council (European Union); the Leventis Foundation; the A. P. Sloan Foundation; the Alexander von Humboldt Foundation; the Belgian Federal Science Policy Office; the Fonds pour la Formation à la Recherche dans l’Industrie et dans l’Agriculture (FRIA-Belgium); the Agentschap voor Innovatie door Wetenschap en Technologie (IWT-Belgium); the Ministry of Education, Youth and Sports (MEYS) of Czech Republic; the Council of Science and Industrial Research, India; the Compagnia di San Paolo (Torino); and the HOMING PLUS program of Foundation for Polish Science, cofinanced from European Union, Regional Development Fund; and the Thalis and Aristeia programmes cofinanced by EU-ESF and the Greek NSRF.Article funded by SCOAP3.Peer Reviewe