128 research outputs found
Intestinal microbiota transplantation to germ-free mice in a in vivo model of nafld associated with a quercetin treatment
15 p.To select mice donors for intestinal microbiota transplantation
based on its metabolic phenotype in response to a high fat diet (HFD) and quercetin treatment (Q). Intestinal microbiota. Resumen de un trabajo resultado del proyecto de investigación financiado por la Consejería de Educación de la Junta de Castilla y León (referencia LE063U16)S
Protective effect of quercetin on high-fat diet-induced non-alcoholic fatty liver disease in mice is mediated by modulating intestinal microbiota imbalance and related gut-liver axis activation
60 p.Gut microbiota is involved in obesity, metabolic syndrome and the progression of
nonalcoholic fatty liver disease (NAFLD). It has been recently suggested that the
flavonoid quercetin may have the ability to modulate the intestinal microbiota
composition, suggesting a prebiotic capacity which highlights a great therapeutic
potential in NAFLD. The present study aims to investigate benefits of experimental
treatment with quercetin on gut microbial balance and related gut-liver axis activation in
a nutritional animal model of NAFLD associated to obesity. C57BL/6J mice were
challenged with high fat diet (HFD) supplemented or not with quercetin for 16 weeks.
HFD induced obesity, metabolic syndrome and the development of hepatic steatosis as
main hepatic histological finding. Increased accumulation of intrahepatic lipids was
associated with altered gene expression related to lipid metabolism, as a result of
deregulation of their major modulators. Quercetin supplementation decreased insulin
resistance and NAFLD activity score, by reducing the intrahepatic lipid accumulation
through its ability to modulate lipid metabolism gene expression, cytochrome P450 2E1
(CYP2E1)-dependent lipoperoxidation and related lipotoxicity. Microbiota composition
was determined via 16S ribosomal RNA Illumina next-generation sequencing.
Metagenomic studies revealed HFD-dependent differences at phylum, class and genus
levels leading to dysbiosis, characterized by an increase in Firmicutes/Bacteroidetes
ratio and in Gram-negative bacteria, and a dramatically increased detection of
Helicobacter genus. Dysbiosis was accompanied by endotoxemia, intestinal barrier
dysfunction and gut-liver axis alteration and subsequent inflammatory gene
overexpression. Dysbiosis-mediated toll-like receptor 4 (TLR-4)-NF-B signaling
pathway activation was associated with inflammasome initiation response and reticulum
stress pathway induction. Quercetin reverted gut microbiota imbalance and related
endotoxemia-mediated TLR-4 pathway induction, with subsequent inhibition of
inflammasome response and reticulum stress pathway activation, leading to the
blockage of lipid metabolism gene expression deregulation. Our results support the
suitability of quercetin as a therapeutic approach for obesity-associated NAFLD via its
anti-inflammatory, antioxidant and prebiotic integrative response.Gut microbiota is involved in obesity, metabolic syndrome and the progression of
nonalcoholic fatty liver disease (NAFLD). It has been recently suggested that the
flavonoid quercetin may have the ability to modulate the intestinal microbiota
composition, suggesting a prebiotic capacity which highlights a great therapeutic
potential in NAFLD. The present study aims to investigate benefits of experimental
treatment with quercetin on gut microbial balance and related gut-liver axis activation in
a nutritional animal model of NAFLD associated to obesity. C57BL/6J mice were
challenged with high fat diet (HFD) supplemented or not with quercetin for 16 weeks.
HFD induced obesity, metabolic syndrome and the development of hepatic steatosis as
main hepatic histological finding. Increased accumulation of intrahepatic lipids was
associated with altered gene expression related to lipid metabolism, as a result of
deregulation of their major modulators. Quercetin supplementation decreased insulin
resistance and NAFLD activity score, by reducing the intrahepatic lipid accumulation
through its ability to modulate lipid metabolism gene expression, cytochrome P450 2E1
(CYP2E1)-dependent lipoperoxidation and related lipotoxicity. Microbiota composition
was determined via 16S ribosomal RNA Illumina next-generation sequencing.
Metagenomic studies revealed HFD-dependent differences at phylum, class and genus
levels leading to dysbiosis, characterized by an increase in Firmicutes/Bacteroidetes
ratio and in Gram-negative bacteria, and a dramatically increased detection of
Helicobacter genus. Dysbiosis was accompanied by endotoxemia, intestinal barrier
dysfunction and gut-liver axis alteration and subsequent inflammatory gene
overexpression. Dysbiosis-mediated toll-like receptor 4 (TLR-4)-NF-B signaling
pathway activation was associated with inflammasome initiation response and reticulum
stress pathway induction. Quercetin reverted gut microbiota imbalance and related
endotoxemia-mediated TLR-4 pathway induction, with subsequent inhibition of
inflammasome response and reticulum stress pathway activation, leading to the
blockage of lipid metabolism gene expression deregulation. Our results support the
suitability of quercetin as a therapeutic approach for obesity-associated NAFLD via its
anti-inflammatory, antioxidant and prebiotic integrative respons
Intestinal Microbiota Transplantation From HFD-fed and Quercetin Treated Donors Results in a Complex Metabolic Phenotype Transfer that Modulates Obesity-Related NAFLD in Germ Free Mice
2 p.Intestinal microbiota imbalance and related
gut-liver axis activation have been identified as key mechanisms
in nonalcoholic fatty liver disease (NAFLD) development.
Modulation of intestinal microbiota, through
administration of prebiotics or faecal microbiota transplantation,
is a promising therapeutic approach for obesity
associated diseases including NAFLD. The aim of the
present study is to evaluate the benefits of gut microbiota
transplantation from donors to germ free mice (GFm)
following an experimental treatment with the flavonoid
quercetin in a high fat diet (HFD)-based NAFLD model. Resumen de un trabajo resultado del proyecto de investigación financiado por la Consejería de Educación de la Junta de Castilla y León (referencia LE063U16)S
Metabolic Phenotype Transfer Through Gut Microbiota Transplantation from HFD-Fed and Quercetin Treated Donors Modulates Obesity-Related NAFLD in Germ-Free Mice
1 p.Gut microbiota is involved in obesity,
metabolic syndrome and nonalcoholic fatty liver disease
(NAFLD). Strategies to modulate it, including faecal transplantation
and administration of prebiotics as quercetin, are actively
being examined. The present study aims to investigate benefits
of experimental gut microbiota transfer from donors to
germ-free mice (GFm) in a high-fat diet (HFD)-based NAFLD
model. Resumen de un trabajo resultado del proyecto de investigación financiado por la Consejería de Educación de la Junta de Castilla y León (referencia LE063U16)S
Vascular Calcification and Oxidative DNA Damage as Nontraditional Cardiovascular Risk Factors in Chronic Renal Disease
The number of CKD sufferers that require renal replacement techniques (RRTs) is increasing. The severity of cardiovascular disease (CVD) is disproportionate in these kinds of patients and contributes considerably to mortality in CKD patients. We evaluated the association between oxidative DNA damage, antioxidant activity and vascular calcification (VC) in CKD. An analytical cross-sectional study was performed. Two simple plaques were taken for each patient (pelvis-hip, and hands-wrists). The presence of VC was scored as presence (1) and absence (0). Oxidative stress was determined by activity of catalase, superoxide dismutase (SOD) and oxidative DNA damage by determination of 8-OHdG marker. Eighty-one patients were included. The RRT type was similar for hemodialysis (HD) and peritoneal dialysis (PD). Thirty-eight patients (47%) presented VC (p < 0.01); in 61%, the VC was severe (≥3 points). VC prevalence in women was significantly higher, (67%) (p < 0.001), and (29%) men. Sixty four percent of the patients submitted to HD presented VC and 27% to PD (p < 0.001). The activity of the catalase enzyme was significantly decreased in CKD vs. the healthy control (HC) (p < 0.0001). The oxidative DNA damage in CKD was greater vs. HC (p < 0.0001). In conclusion, the VC was frequent (47%) in CKD, and decreased catalase activity and greater oxidative DNA damage
Effect of exercise on gut microbiota and metabolic status modulation in an in vivo model of early obesity and NAFLD
1 p.Childhood obesity is one of the most serious
public health concerns from this century, associated with metabolic
syndrome, nonalcoholic fatty liver disease (NAFLD) and gut microbiota
alterations. Physical exercise improves obesity and NAFLD
progression, modulating the gut microbial balance. We aim to
investigate the effect of physical exercise on gut microbiota and the
metabolic status of an in vivo model of early obesity, metabolic
syndrome, and NAFLD. Resumen de un trabajo resultado del proyecto de investigación financiado por la Consejería de Educación de la Junta de Castilla y León (referencia LE063U16)S
Protective effect of quercetin treatment on HFD-induced intestinal dysbiosis and barrier dysfunction in an in vivo model of non-alcoholic fatty liver disease
1 p.Gut microbiota is involved in obesity,
metabolic syndrome and nonalcoholic fatty liver disease (NAFLD).
Quercetin may have the ability to modulate the intestinal microbiota
composition, suggesting therapeutic potential in NAFLD. The present
study aims to investigate the beneficial effect of quercetin treatment
on dysbiosis, intestinal barrier dysfunction and gut-liver axis
alteration in high-fat diet (HFD)-fed mice. Resumen de un trabajo resultado del proyecto de investigación financiado por la Consejería de Educación de la Junta de Castilla y León (referencia LE063U16)S
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