15 p.To select mice donors for intestinal microbiota transplantation
based on its metabolic phenotype in response to a high fat diet (HFD) and quercetin treatment (Q). Intestinal microbiota. Resumen de un trabajo resultado del proyecto de investigación financiado por la Consejería de Educación de la Junta de Castilla y León  (referencia LE063U16)S

García-Mediavilla, María Victoria

González-Gallego, Javier

Jorquera, F.

Martínez-Flórez, Susana

Nistal, María Esther

Pisonero-Vaquero, Sandra

Porras, David

Sánchez-Campos, Sonia

English

Leon University (Spain)

SEIQ abstracts 15Conclusions: Although results cannot be generalized due to a small sample,surgeons adopted different postures during the intervention while using eachtype of laparoscopic needle holder.Acknowledgements. This study was supported by the Government of Extremadura,Spain, the European Social Fund (PO14034) and the European Regional DevelopmentFund (CTS037).Intestinal microbiota transplantation to germ-free mice in a in vivomodel of nafld associated with a quercetin treatmentD. Porras1, M.V. García-Mediavilla1,2, E. Nistal1,3, S. Martínez-Flórez1,F. Jorquera1,2,3, S. Pisonero-Vaquero1, J. González-Gallego1,2,S. Sánchez-Campos1,21Institute of Biomedicine (IBIOMED), University of León, León,; 2Centro de Investi-gación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Institutode Salud Carlos III, 3Departament of Gastroenterology, Complejo Asistencial Univer-sitario de León, León. SpainBackground: Gut microbiota is involved in obesity, metabolic syndrome (MS)and nonalcoholic fatty liver disease (NAFLD).Objectives: To select mice donors for intestinal microbiota transplantationbased on its metabolic phenotype in response to a high fat diet (HFD) andquercetin treatment (Q). Intestinal microbiota transplantation from donors togerm-free mice under the same experimental model.Methods: HFD with or without quercetin feeding C57BL/6J mice wereeuthanized after 16 weeks, and blood, liver, and caecal samples were collected.Liver histological studies were carried out. Liver function and plasma lipid andglucose levels were analyzed.Results: HFD mice showed typical pathophysiological features of NAFLD,with obesity, MS, steatosis and liver damage. HFD caused dysbiosis as indi-cated by the reduction of total number of bacteria and Firmitutes/Bacteroidetesrate increase. Quercetin tended to normalize these parameters, showing pre-biotic capacity. Donors of intestinal microbiota were selected on the basis ofbody weight gain, MS, histological findings and altered gut-liver axis. Miceresponder (HFD+) and non-responder (HFD-) to HFD together with a con-trol, a ControlQ, and a HFDQ (responder to quercetin) were selected as donors.Germ-free mice were colonised with gut microbiota from donors and dividedinto 20 groups according to the transplanted feces and diets (Control, Con-trolQ, HFD or HFDQ) in order to establish the metabolic phenotype transferin response to the HFD diet and quercetin.Conclusions HFD induced different metabolic phenotypes allowing tothe selection of donors of intestinal microbiota from HFD responders andnon-responders. Also, quercetin supplementation modified the metabolicphenotype of HFD mice.Supported by BFU2013-48141-R, LEU35U13, LE063U16 (Junta de Castilla y Leóny Fondo Europeo de Desarrollo Regional (FEDER)) y GRS 1428/A/16. CIBERehd estáfinanciado por el Instituto de Salud Carlos III.Pressure ulcers treatment in paraplegic patients using bone-marrowautologous mononuclear cells vs combined with platelet-rich plasmaS. Pérez López, M. Pérez Basterrechea , M. Álvarez Viejo , A. FerreroGutiérrez, J.M. García Gala, Á. Pérez Arias, Á. Fernández Rodríguez,M.L. López Llano, J. Otero HernándezUnit of Transplants, Cell Therapy and Regenerative Medicine, University Hospitalof Asturias, Oviedo, SpainObjectives: Pressure ulcers are a major health problem for bedridden patientsor persons with reduced mobility. In patients with SCI, ulcers are often a chronicproblem and approximately 85% of these patients will develop pressure ulcerssometime during their lives. Pressure ulcers are difficult to treat with standardmedical therapy and often they recur. Taking into account preliminary positiveresults obtained with autologous bone marrow mononuclear cells (BM-MNCs)and published by our group, an alternative approach was considered in orderto heal ulcers that are not able to be treated with this method due to their areaand/or dimensions.Methods: 29 patients with type IV chronic pressure ulcers were included in thestudy. All these patients had undergone prior surgery with a lack of response.The bursa was washed with saline solution and the wound was debrided.After this intervention the patients were divided into two groups dependingon the pressure ulcer characteristics. One group named BM-MNCs (n= 11,x= 51⋅09 years) received a MNCs suspension obtained from autologous bonemarrow. A second group called PRP (n= 18, x= 55,27) received a combinationof autologous BM-MNCs and PRP obtained from peripheral blood from thesame patient. Finally, the ulcer margins were revitalized and the wound closedby a purse-string silk suture.Results: The mean number of MNCs infused in each group was 104,44 ⋅ 106for BM-MNCs y 123,25 ⋅ 106 for PRP group. Ulcer resolution was 72⋅72% forthe first group and 78⋅95% for the second group. The mean time to completeclosure was 37,66 days in the BM-MNCs group and 44,23 days for PRP group.During follow-up (44 months) none of the patients whose wounds had resolvedunderwent ulcer recurrence.Conclusions: Cell therapy using autologous BM-MNCs combined with PRPseems to be an adequate option to treat type IV chronic pressure ulcers whoseparticular morphology needs an alternative method. Moreover, the mean timeperiod to achieve the wound closure is similar in the two groups.Genetic changes in a new animal model of human pancreatic cancerbased on mesenchymal cells versus a classic model based on peritu-moral fibroblastsJ. F. Vázquez-Villa1,2, C. Barneo-Caragol3, R. Rodríguez-Uría4,S. Sanz-Navarro4, E. Fernando-Macías1, B. Porrero-Guerrero1,C. López-Arévalo1, J.R. de los Toyos2,5, A. Gómez-Pinillos1, L. Barneo1,2,41Faculty of Medicine and Health Sciences, Department of Surgery and Surgical MedicalSpecialties, University of Oviedo, Spain; 2University Institute of Oncology of Asturias,Spain; 3Clinical Biochemistry Service, Central University Hospital of Asturias, Oviedo,Spain; 4General and Digestive Surgery, Central University Hospital of Asturias,Oviedo, Spain; 5Faculty of Medicine and Health Science, Functional Biology Depart-ment, University of Oviedo, SpainObjectives: We have described two animal models of human pancreatic can-cer developed by orthotopic transplantation. The new model uses immor-talized human mesenchymal cells (IHBMMC), whereas peritumoral fibrob-lasts (FPT) were used for the classic model. Both models show large tumorgrowth with metastatic dissemination. In this paper, we try to determinethe differences between both xenograft models in the expression of humangenes involved in tumor development of Hedgehog signaling pathway and theepithelial-mesenchymal transition (EMT) process.Methods: Five experimental groups of severe combined immunodeficiency(SCID) transplant recipient mice were established with: IHBMMC (Group I),FPT (Group II), Capan-1 tumor cells (Group III), IHBMMC and Capan-1(Group IV), and FPT and Capan-1 (group V). From 24 pancreas tissue samples,mRNA expression levels were determined by qPCR of the Hedgehog and EMTpathways.Results: Statistical analysis of the data in the new model, based on theco-transplantation IHBMMC and Capan-1, shows that Hedgehog pathwaySHH and EMT process SNAI1 genes are overexpressed when compared to thelevels detected in recipient mice Capan-1. In the classical model based on FPTand Capan-1, the expression profile is similar, although the aforementionedgenes fail to be overexpressed.Conclusions: The gene expression pattern of the new animal model ofpancreatic cancer obtained using immortalized mesenchymal cells is differentfrom that generated with peritumoral fibroblasts. Specifically, the co-transplantof IHBMMC and Capan-1 cells stimulates the expression of SHH and SNAILgenes.Grants: MICINN-10-IPT-010000-2010-31 y GRUPIN14-121© 2017 The Authors www.bjs.co.uk BJS 2017; 104 (S1): 5–25BJS © 2017 BJS Society Ltd

Intestinal microbiota transplantation to germ-free mice in a in vivo model of nafld associated with a quercetin treatment

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Intestinal microbiota transplantation to germ-free mice in a in vivo model of nafld associated with a quercetin treatment

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Leon University (Spain)