Development of a reproducible and optimized synthetic protocol for the preparation of monodisperse core-shell-type magnetic mesoporous silica nanoparticles

[ES] La fabricación de nanopartículas con tamaños por debajo de los 100 nm ha permitido el desarrollo de innovadores nanodispositivos capaces de interactuar de forma directa con sistemas vivos a nivel celular y molecular, convirtiéndose en una parte fundamental dentro del campo de la nanomedicina. Uno de los principales retos a los que se enfrenta la ingeniería de nanopartículas es el desarrollo de nanodispositivos con propiedades físico-químicas bien definidas, ya que de ellas depende el comportamiento y biodistribución de dichos sistemas una vez introducidos en el organismo. No menos importante es el desarrollo de protocolos de síntesis reproducibles y optimizados, indispensables para la fabricación y escalado de nanodispositivos que puedan ser trasladados a futuras aplicaciones biomédicas. El principal objetivo de este proyecto de doctorado es el estudio y fabricación de nanopartículas magnéticas mesoporosas de sílice con estructura "core-shell" para su aplicación como agentes teranósticos en el campo de la nanomedicina. En este estudio se analiza en profundidad la síntesis y caracterización de dichos nanomateriales con el objetivo de producir nanopartículas con unas propiedades físico-químicas bien definidas de forma controlada y reproducible. La obtención de dichas nanopartículas supondría un gran avance de cara al desarrollo de nanodispositivos más complejos y sofisticados. El contenido de la tesis se ha estructurado en distintos capítulos que se detallan brevemente a continuación: ¿El capítulo 1 es una introducción a la nanomedicina, destacando el papel fundamental que tienen las nanopartículas en el desarrollo de nuevas aplicaciones biomédicas. A continuación se presentan las nanopartículas de sílice mesoporosa, mostrando la gran versatilidad de dichos nanomateriales para el desarrollo de dispositivos teranósticos así como sistemas para la liberación controlada de fármacos. Por último, se destaca la importancia de fabricar nanodispositivos con unas propiedades físico-químicas bien definidas como requisito indispensable para la traslación de los resultados experimentales hacia el campo clínico. ¿El capítulo 2 incluye los objetivos principales de la tesis. ¿El capítulo 3 se centra en la síntesis y caracterización de nanopartículas superparamagnéticas de óxido de hierro (USPIONs), siendo estas utilizadas en capítulos posteriores para la síntesis de las nanopartículas mesoporosas tipo "core-shell". Las USPIONs son preparadas a través de un método sencillo de coprecipitación en el que se emplean condiciones de reacción moderadas. Las nanopartículas obtenidas son caracterizadas en profundidad, analizando sus propiedades magnéticas para su aplicación en hipertermia magnética y como agentes de contraste dual en imagen por resonancia magnética (MRI). ¿El capítulo 4 está dedicado a la preparación de nanopartículas magnéticas mesoporosas de sílice con estructura "core-shell". Los conceptos fundamentales relacionados con los mecanismos de formación de este tipo de nanomateriales son ampliamente analizados, así como los parámetros de reacción involucrados en la síntesis. Como punto de partida, se propone un protocolo de síntesis general para la obtención de las nanopartículas tipo "core-shell". A continuación, se analiza en profundidad el efecto que los distintos parámetros de reacción tienen en las propiedades físico-químicas de dichas nanopartículas. Para la fase de optimización se utiliza un modelo semi-empírico como referencia, racionalizando los resultados experimentales observados en base a un posible mecanismo de formación. ¿El capítulo 5 se centra en el análisis y caracterización de la estructura mesoporosa de las nanopartículas tipo "core-shell". Además, se analiza el efecto que los distintos parámetros de reacción tienen sobre la estructura final de las nanopartículas, aportando información adicional sobre su posible mecanismo[CA] La fabricació de nanopartícules amb grandàries per davall dels 100 nm ha permés el desenvolupament d'innovadors nanodispositius capaços d'interactuar de forma directa amb sistemes vius a nivell cel¿lular i molecular, convertint-se en una part fonamental dins del camp de la nanomedicina. Un dels principals reptes als quals s'enfronta l'enginyeria de nanopartícules és el desenvolupament de nanodispositius amb propietats físic-químiques ben definides, ja que d'elles depén el comportament i biodistribució d'aquests sistemes una vegada introduïts en l'organisme. No menys important és el desenvolupament de protocols de síntesis reproduïbles i optimitzats, indispensables per a la fabricació a gran escala de nanodispositius que puguen ser utilitzats en futures aplicacions biomèdiques. El principal objectiu d'aquest projecte de doctorat és l'estudi i fabricació de nanopartícules magnètiques mesoporoses de sílice amb estructura "core-shell" per a la seua aplicació com a agents teranòstics en el camp de la nanomedicina. En aquest estudi s'analitza en profunditat la síntesi i caracterització d'aquests nanomaterials amb l'objectiu de produir nanopartícules amb unes propietats físic-químiques ben definides de forma controlada i reproduïble. L'obtenció d'aquestes nanopartícules suposaria un gran avanç de cara al desenvolupament de nanodispositius més complexos i sofisticats. El contingut de la tesi s'ha estructurat en diferents capítols que es detallen breument a continuació: ¿El capítol 1 és una introducció a la nanomedicina, destacant el paper fonamental que tenen les nanopartícules en el desenvolupament de noves aplicacions biomèdiques. A continuació es presenten les nanopartícules de sílice mesoporosa, mostrant la gran versatilitat d'aquests nanomaterials per al desenvolupament de dispositius teranòstics així com sistemes per a l'alliberament controlat de fàrmacs. Finalment, es destaca la importància de fabricar nanodispositius amb unes propietats físic-químiques ben definides com a requisit indispensable per a la translació dels resultats experimentals al camp clínic. ¿El capítol 2 inclou els objectius principals de la tesi així com els objectius específics proposats per a cada capítol de la tesi. ¿El capítol 3 està dedicat a la síntesi i caracterització de nanopartícules superparamagnétiques d'òxid de ferro (USPIONs), sent aquestes utilitzades en capítols posteriors per a la síntesi de les nanopartícules mesoporoses tipus "core-shell". Les USPIONs són preparades a través d'un mètode senzill de coprecipitació en el qual s'empren condicions de reacció moderades. Les nanopartícules obtingudes són caracteritzades en profunditat, analitzant les seues propietats magnètiques per a la seua aplicació en hipertèrmia magnètica i com a agents de contrast dual en imatge per ressonància magnètica (MRI). ¿El capítol 4 està dedicat a la preparació de nanopartícules magnètiques mesoporoses de sílice amb estructura "core-shell". Els conceptes fonamentals relacionats amb els mecanismes de formació d'aquest tipus de nanomaterials són àmpliament analitzats, així com els paràmetres de reacció involucrats en la síntesi. Com a punt de partida, es proposa un protocol de síntesi general per a l'obtenció de les nanopartícules tipus "core-shell". A continuació, s'analitza en profunditat l'efecte que els diferents paràmetres de reacció tenen en les propietats físic-químiques d'aquestes nanopartícules. Per a la fase d'optimització s'utilitza un model semi-empíric com a referència, racionalitzant els resultats experimentals observats sobre la base d'un possible mecanisme de formació. ¿El capítol 5 està dedicat a l'anàlisi i caracterització de l'estructura mesoporosa de les nanopartícules tipus "core-shell". A més, s'analitza l'efecte que els diferents paràmetres de reacció tenen sobre l'estructura final de les nanopartícules, aportant informació[EN] The fabrication of nanoparticles with sizes below 100 nm has opened the door to the development of innovative nanodevices that directly interact with living systems at the cellular and molecular level, becoming an essential part of nanomedicine. One of the main challenges that nanoparticle engineering is currently facing is the design of nanodevices with well-defined physico-chemical properties, which ultimately determine the fate and function of these systems inside the organism. Similarly, the development of reproducible and versatile synthetic protocols is of great importance for manufacture purposes, a fundamental requirement for an efficient translation of this technology into the clinic. The main objective of this PhD thesis is the study and fabrication of core-shell-type magnetic mesoporous silica nanoparticles (M-MSNs) for their application as theranostic nanodevices in the field of nanomedicine. A comprehensive study about the synthesis and characterization of this type of nanomaterials is presented with the aim of obtaining core-shell M-MSNs with well-defined physico-chemical properties in a robust and reproducible way. The fabrication of such particles would provide a versatile and reliable platform for the development of more complex nanodevices with advanced functionalities. The thesis has been structured into several chapters that are briefly summarized as follows: ¿Chapter 1 is an introduction to the topic of nanomedicine, highlighting the importance of nanoparticles in the development of new biomedical applications. Mesoporous silica nanoparticles are then introduced, showing the great versatility that this nanomaterials offer for the development of theranostic nanodevices and smart drug delivery systems. Finally, the development of nanodevices with well-defined physico-chemical properties is identified as a crucial requirement for overcoming biological barriers and facilitate the translation of nanomedicines from the bench to bedside. ¿Chapter 2 presents the aims of this thesis and the specific objectives that are addressed in the following chapters. ¿Chapter 3 is devoted to the synthesis and characterization of ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs), which are later used as magnetic seeds for the synthesis of core-shell M-MSNs. USPIONs are prepared through a simple coprecipitation method using mild reaction conditions. The obtained nanoparticles are fully characterized and their magnetic properties are analyzed focusing on magnetic hyperthermia and dual MR imaging applications. ¿Chapter 4 is a comprehensive study about the preparation of monodisperse core-shell M-MSNs. The main concepts related to the synthesis and formation mechanisms of this type of nanomaterials are revised, together with the reaction parameters that are expected to have a major contribution on the reaction. As a starting point, a general synthetic protocol for the synthesis of core-shell M-MSNs is presented. Then, specific reaction parameters are investigated in order to understand their effect on the physico-chemical properties of the obtained nanoparticles. The application of a semi-empirical model to the optimization stage is presented in an attempt to provide an adequate reference framework to understand the formation of this complex nanodevices. ¿Chapter 5 presents a detailed analysis about the characterization of mesoporous silica materials and, in particular, the assessment of the mesoporous structure of MSNs with a radial distribution of wormhole-like channels. The effects that specific reaction parameters have on the mesoporous silica structure of core-shell M-MSNs are also analysed, providing additional information about the formation of this type of nanoparticles. ¿Chapter 6 gathers the main conclusions of this thesis.Sánchez Cabezas, S. (2019). Development of a reproducible and optimized synthetic protocol for the preparation of monodisperse core-shell-type magnetic mesoporous silica nanoparticles [Tesis doctoral no publicada]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/129878TESI

Indirect calculation of monoclonal antibodies in nanoparticles using the radiolabeling process with technetium 99 metastable as primary factor: Alternative methodology for the entrapment efficiency

[EN] The use of monoclonal antibodies (Mab) in the current medicine is increasing. Antibody-drug conjugates (ADCs) represents an increasingly and important modality for treating several types of cancer. In this area, the use of Mab associated with nanoparticles is a valuable strategy. However, the methodology used to calculate the Mab entrapment, efficiency and content is extremely expensive. In this study we developed and tested a novel very simple one-step methodology to calculate monoclonal antibody entrapment in mesoporous silica (with magnetic core) nanoparticles using the radiolabeling process as primary methodology. The magnetic core mesoporous silica were successfully developed and characterised. The PXRD analysis at high angles confirmed the presence of magnetic cores in the structures and transmission electron microscopy allowed to determine structures size (58.9 +/- 8.1 nm). From the isotherm curve, a specific surface area of 872 m(2)/g was estimated along with a pore volume of 0.85 crn(3)/g and an average pore diameter of 3.15 nm. The radiolabeling process to proceed the indirect determination were well-done. Trastuzumab were successfully labeled (>97%) with Tc-99m generating a clear suspension. Besides, almost all the Tc-99m used (labeling the trastuzumab) remained trapped in the surface of the mesoporous silica for a period as long as 8 h. The indirect methodology demonstrated a high entrapment in magnetic core mesoporous silica surface of Tc-99m-traztuzumab. The results confirmed the potential use from the indirect entrapment efficiency methodology using the radiolabeling process, as a one-step, easy and cheap methodology. (C) 2018 Elsevier B.V. All rights reserved.The authors would like to thank the National Scientific and Technological Research Council (CNPQ) and the Rio de Janeiro State Research Foundation (FAPERJ) for funding. Authors also gratefully acknowledge the financial support from the Ministerio de Economia y Competitividad (Project MAT2012-38429-004-01) and the Generalitat Valenciana (project PROMETEO/2009/016) for support.Helal-Neto, E.; Sánchez-Cabezas, S.; Sancenón Galarza, F.; Martínez-Máñez, R.; Santos-Oliveira, R. (2018). Indirect calculation of monoclonal antibodies in nanoparticles using the radiolabeling process with technetium 99 metastable as primary factor: Alternative methodology for the entrapment efficiency. Journal of Pharmaceutical and Biomedical Analysis. 153:90-94. https://doi.org/10.1016/j.jpba.2018.02.017S909415

Influence of Medium Viscosity and Intracellular Environment on the Magnetization of Superparamagnetic Nanoparticles in Silk Fibroin Solutions and 3T3 Mouse Fibroblast Cell Cultures

IOP also requests that you include the following statement of provenance: "This is an author-created, un-copyedited versíon of an article published in Nanotechnology. IOP Publishing Ltd is not responsíble for any errors or omissíons in this versíon of the manuscript or any versíon derived from it. The Versíon of Record is available online at https://doi.org/10.1088/1361-6528/aacf4a.[EN] Biomedical applications based on the magnetic properties of superparamagnetic iron oxide nanoparticles (SPIONs) may be altered by the mechanical attachment or cellular uptake of these nanoparticles. When nanoparticles interact with living cells, they are captured and internalized into intracellular compartments. Consequently, the magnetic behavior of the nanoparticles is modified. In this paper, we investigated the change in the magnetic response of 14 nm magnetic nanoparticles (Fe3O4) in different solutions, both as a stable liquid suspension (one of them mimicking the cellular cytoplasm) and when associated with cells. The field-dependent magnetization curves from inert fluids and cell cultures were determined by using an alternating gradient magnetometer, MicroMagTM 2900. The equipment was adapted to measure liquid samples because it was originally designed only for solids. In order to achieve this goal, custom sample holders were manufactured. Likewise, the nuclear magnetic relaxation dispersion profiles for the inert fluid were also measured by fast field cycling nuclear magnetic relaxation relaxometry. The results show that SPION magnetization in inert fluids was affected by the carrier liquid viscosity and the concentration. In cell cultures, the mechanical attachment or confinement of the SPIONs inside the cells accounted for the change in the dynamic magnetic behavior of the nanoparticles. Nevertheless, the magnetization value in the cell cultures was slightly lower than that of the fluid simulating the viscosity of cytoplasm, suggesting that magnetization loss was not only due to medium viscosity but also to a reduction in the mechanical degrees of freedom of SPIONs rotation and translation inside cells. The findings presented here provide information on the loss of magnetic properties when nanoparticles are suspended in viscous fluids or internalized in cells. This information could be exploited to improve biomedical applications based on magnetic properties such as magnetic hyperthermia, contrast agents and drug delivery.The authors are thankful to their supporters: a grant from Universidad Politecnica de Madrid to Ana Lorena Urbano-Bojorge and a grant from Universidad Nacional Experimental del Tachira (UNET)- Venezuela to Oscar Casanova-Carvajal. This study was also financially supported in part by CIBER-BBN (Spain) and Madr.ib-CM (Spain).Urbano-Bojorge, AL.; Casanova-Carvajal, O.; González, N.; Fernández, L.; Madurga, R.; Sánchez-Cabezas, S.; Aznar, E.... (2018). Influence of Medium Viscosity and Intracellular Environment on the Magnetization of Superparamagnetic Nanoparticles in Silk Fibroin Solutions and 3T3 Mouse Fibroblast Cell Cultures. Nanotechnology. 29(38):1-13. https://doi.org/10.1088/1361-6528/aacf4aS113293

Influence of Medium Viscosity and Intracellular Environment on the Magnetization of Superparamagnetic Nanoparticles in Silk Fibroin Solutions and 3T3 Mouse Fibroblast Cell Cultures

IOP also requests that you include the following statement of provenance: "This is an author-created, un-copyedited versíon of an article published in Nanotechnology. IOP Publishing Ltd is not responsíble for any errors or omissíons in this versíon of the manuscript or any versíon derived from it. The Versíon of Record is available online at https://doi.org/10.1088/1361-6528/aacf4a.[EN] Biomedical applications based on the magnetic properties of superparamagnetic iron oxide nanoparticles (SPIONs) may be altered by the mechanical attachment or cellular uptake of these nanoparticles. When nanoparticles interact with living cells, they are captured and internalized into intracellular compartments. Consequently, the magnetic behavior of the nanoparticles is modified. In this paper, we investigated the change in the magnetic response of 14 nm magnetic nanoparticles (Fe3O4) in different solutions, both as a stable liquid suspension (one of them mimicking the cellular cytoplasm) and when associated with cells. The field-dependent magnetization curves from inert fluids and cell cultures were determined by using an alternating gradient magnetometer, MicroMagTM 2900. The equipment was adapted to measure liquid samples because it was originally designed only for solids. In order to achieve this goal, custom sample holders were manufactured. Likewise, the nuclear magnetic relaxation dispersion profiles for the inert fluid were also measured by fast field cycling nuclear magnetic relaxation relaxometry. The results show that SPION magnetization in inert fluids was affected by the carrier liquid viscosity and the concentration. In cell cultures, the mechanical attachment or confinement of the SPIONs inside the cells accounted for the change in the dynamic magnetic behavior of the nanoparticles. Nevertheless, the magnetization value in the cell cultures was slightly lower than that of the fluid simulating the viscosity of cytoplasm, suggesting that magnetization loss was not only due to medium viscosity but also to a reduction in the mechanical degrees of freedom of SPIONs rotation and translation inside cells. The findings presented here provide information on the loss of magnetic properties when nanoparticles are suspended in viscous fluids or internalized in cells. This information could be exploited to improve biomedical applications based on magnetic properties such as magnetic hyperthermia, contrast agents and drug delivery.The authors are thankful to their supporters: a grant from Universidad Politecnica de Madrid to Ana Lorena Urbano-Bojorge and a grant from Universidad Nacional Experimental del Tachira (UNET)- Venezuela to Oscar Casanova-Carvajal. This study was also financially supported in part by CIBER-BBN (Spain) and Madr.ib-CM (Spain).Urbano-Bojorge, AL.; Casanova-Carvajal, O.; González, N.; Fernández, L.; Madurga, R.; Sánchez-Cabezas, S.; Aznar, E.... (2018). Influence of Medium Viscosity and Intracellular Environment on the Magnetization of Superparamagnetic Nanoparticles in Silk Fibroin Solutions and 3T3 Mouse Fibroblast Cell Cultures. Nanotechnology. 29(38):1-13. https://doi.org/10.1088/1361-6528/aacf4aS113293

Planificación del trabajo psicológico en equipos de fútbol: Equipo técnico de psicólogos Sevilla F.C.

El Equipo Técnico de Psicólogos comenzó su labor en la temporada 1998-1999, cuando Miguel Morilla diseñó un proyecto de trabajo psicológico que desde entonces se ha desarrollado ininterrumpidamente (Morilla et al., 2009). El grupo está compuesto por 8 profesionales para la temporada 2009-2010 y sus objetivos se enmarcan en la línea de los fijados con carácter general para los escalafones inferiores. En ese sentido se propone un desarrollo armónico dentro de un proceso global, en el cual se integra la preparación mental a la técnica, la táctica y el entrenamiento físico. Así mismo, se vela por el adecuado crecimiento formativo y académico, paralelo al deportivo. Como resultado del trabajo psicológico, se observan mejoras en el desarrollo de habilidades psicológicas y en el rendimiento académico de los futbolistas, se han publicado diferentes libros y cuadernillos, y se han institucionalizado diferentes servicios dentro del Club

Combining magnetic hyperthermia and dual T1/T2 MR imaging using highly versatile iron oxide nanoparticles

[EN] Magnetic hyperthermia and magnetic resonance imaging (MRI) are two of the most important biomedical applications of magnetic nanoparticles (MNPs). However, the design of MNPs with good heating performance for hyperthermia and dual T1/T2 contrast for MRI remains a considerable challenge. In this work, ultrasmall superparamagnetic iron oxide nanoparticles (USPIONs) are synthesized through a simple one-step methodology. A post-synthetic purification strategy has been implemented in order to separate discrete nanoparticles from aggregates and unstable nanoparticles, leading to USPIONs that preserve chemical and colloidal stability for extended periods of time. The optimized nanoparticles exhibit high saturation magnetization and show good heating efficiency in magnetic hyperthermia experiments. Remarkably, the evaluation of the USPIONs as MRI contrast agents revealed that the nanoparticles are also able to provide significant dual T1/T2 signal enhancement. These promising results demonstrate that USPIONs are excellent candidates for the development of theranostic nanodevices with potential application in both hyperthermia and dual T1/T2 MR imaging.We are grateful to the Spanish Government (projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R (MINECO/FEDER)) and the Generalitat Valenciana (Projects PROMETEO/2018/024 and PROMETEOII/2014/047) for financial support. S. S. C. is grateful to the Spanish MEC for his FPU grant. JG acknowledges funding from FCT and the ERDF through NORTE2020 through the project Self-reporting immunestimulating formulation for on-demand cancer therapy with real-time treatment response monitoring (028052).Sánchez-Cabezas, S.; Montes-Robles, R.; Gallo, J.; Sancenón Galarza, F.; Martínez-Máñez, R. (2019). Combining magnetic hyperthermia and dual T1/T2 MR imaging using highly versatile iron oxide nanoparticles. Dalton Transactions. 48(12):3883-3892. https://doi.org/10.1039/c8dt04685aS388338924812Lee, J.-H., Jang, J., Choi, J., Moon, S. H., Noh, S., Kim, J., … Cheon, J. (2011). Exchange-coupled magnetic nanoparticles for efficient heat induction. Nature Nanotechnology, 6(7), 418-422. doi:10.1038/nnano.2011.95Hauser, A. K., Wydra, R. J., Stocke, N. A., Anderson, K. W., & Hilt, J. Z. (2015). Magnetic nanoparticles and nanocomposites for remote controlled therapies. Journal of Controlled Release, 219, 76-94. doi:10.1016/j.jconrel.2015.09.039González, B., Ruiz-Hernández, E., Feito, M. J., López de Laorden, C., Arcos, D., Ramírez-Santillán, C., … Vallet-Regí, M. (2011). Covalently bonded dendrimer-maghemite nanosystems: nonviral vectors for in vitro gene magnetofection. Journal of Materials Chemistry, 21(12), 4598. doi:10.1039/c0jm03526bGallo, J., Long, N. J., & Aboagye, E. O. (2013). Magnetic nanoparticles as contrast agents in the diagnosis and treatment of cancer. Chemical Society Reviews, 42(19), 7816. doi:10.1039/c3cs60149hBoyer, C., Whittaker, M. R., Bulmus, V., Liu, J., & Davis, T. P. (2010). The design and utility of polymer-stabilized iron-oxide nanoparticles for nanomedicine applications. NPG Asia Materials, 2(1), 23-30. doi:10.1038/asiamat.2010.6Wáng, Y. X. J., & Idée, J.-M. (2017). A comprehensive literatures update of clinical researches of superparamagnetic resonance iron oxide nanoparticles for magnetic resonance imaging. Quantitative Imaging in Medicine and Surgery, 7(1), 88-122. doi:10.21037/qims.2017.02.09Blanco-Andujar, C., Walter, A., Cotin, G., Bordeianu, C., Mertz, D., Felder-Flesch, D., & Begin-Colin, S. (2016). Design of iron oxide-based nanoparticles for MRI and magnetic hyperthermia. Nanomedicine, 11(14), 1889-1910. doi:10.2217/nnm-2016-5001Shin, T.-H., Choi, Y., Kim, S., & Cheon, J. (2015). Recent advances in magnetic nanoparticle-based multi-modal imaging. Chemical Society Reviews, 44(14), 4501-4516. doi:10.1039/c4cs00345dBusquets, M. A., Estelrich, J., & Sánchez-Martín, M. J. (2015). Nanoparticles in magnetic resonance imaging: from simple to dual contrast agents. International Journal of Nanomedicine, 1727. doi:10.2147/ijn.s76501Lee, N., & Hyeon, T. (2012). Designed synthesis of uniformly sized iron oxide nanoparticles for efficient magnetic resonance imaging contrast agents. Chem. Soc. Rev., 41(7), 2575-2589. doi:10.1039/c1cs15248cWang, G., Zhang, X., Skallberg, A., Liu, Y., Hu, Z., Mei, X., & Uvdal, K. (2014). One-step synthesis of water-dispersible ultra-small Fe3O4 nanoparticles as contrast agents for T1 and T2 magnetic resonance imaging. Nanoscale, 6(5), 2953. doi:10.1039/c3nr05550gKim, B. H., Lee, N., Kim, H., An, K., Park, Y. I., Choi, Y., … Hyeon, T. (2011). Large-Scale Synthesis of Uniform and Extremely Small-Sized Iron Oxide Nanoparticles for High-ResolutionT1Magnetic Resonance Imaging Contrast Agents. Journal of the American Chemical Society, 133(32), 12624-12631. doi:10.1021/ja203340uNegussie, A. H., Yarmolenko, P. S., Partanen, A., Ranjan, A., Jacobs, G., Woods, D., … Dreher, M. R. (2011). Formulation and characterisation of magnetic resonance imageable thermally sensitive liposomes for use with magnetic resonance-guided high intensity focused ultrasound. International Journal of Hyperthermia, 27(2), 140-155. doi:10.3109/02656736.2010.528140Hervault, A., & Thanh, N. T. K. (2014). Magnetic nanoparticle-based therapeutic agents for thermo-chemotherapy treatment of cancer. Nanoscale, 6(20), 11553-11573. doi:10.1039/c4nr03482aKumar, C. S. S. R., & Mohammad, F. (2011). Magnetic nanomaterials for hyperthermia-based therapy and controlled drug delivery. Advanced Drug Delivery Reviews, 63(9), 789-808. doi:10.1016/j.addr.2011.03.008Deatsch, A. E., & Evans, B. A. (2014). Heating efficiency in magnetic nanoparticle hyperthermia. Journal of Magnetism and Magnetic Materials, 354, 163-172. doi:10.1016/j.jmmm.2013.11.006Zhang, J., Li, X., Rosenholm, J. M., & Gu, H. (2011). Synthesis and characterization of pore size-tunable magnetic mesoporous silica nanoparticles. Journal of Colloid and Interface Science, 361(1), 16-24. doi:10.1016/j.jcis.2011.05.038COROT, C., ROBERT, P., IDEE, J., & PORT, M. (2006). Recent advances in iron oxide nanocrystal technology for medical imaging☆. Advanced Drug Delivery Reviews, 58(14), 1471-1504. doi:10.1016/j.addr.2006.09.013Gonzales, M., Mitsumori, L. M., Kushleika, J. V., Rosenfeld, M. E., & Krishnan, K. M. (2010). Cytotoxicity of iron oxide nanoparticles made from the thermal decomposition of organometallics and aqueous phase transfer with Pluronic F127. Contrast Media & Molecular Imaging, 5(5), 286-293. doi:10.1002/cmmi.391Laurent, S., Forge, D., Port, M., Roch, A., Robic, C., Vander Elst, L., & Muller, R. N. (2008). Magnetic Iron Oxide Nanoparticles: Synthesis, Stabilization, Vectorization, Physicochemical Characterizations, and Biological Applications. Chemical Reviews, 108(6), 2064-2110. doi:10.1021/cr068445eKiss, L. B., Söderlund, J., Niklasson, G. A., & Granqvist, C. G. (1999). New approach to the origin of lognormal size distributions of nanoparticles. Nanotechnology, 10(1), 25-28. doi:10.1088/0957-4484/10/1/006De Palma, R., Peeters, S., Van Bael, M. J., Van den Rul, H., Bonroy, K., Laureyn, W., … Maes, G. (2007). Silane Ligand Exchange to Make Hydrophobic Superparamagnetic Nanoparticles Water-Dispersible. Chemistry of Materials, 19(7), 1821-1831. doi:10.1021/cm0628000Roonasi, P., & Holmgren, A. (2009). A Fourier transform infrared (FTIR) and thermogravimetric analysis (TGA) study of oleate adsorbed on magnetite nano-particle surface. Applied Surface Science, 255(11), 5891-5895. doi:10.1016/j.apsusc.2009.01.031Yan, K., Li, H., Wang, X., Yi, C., Zhang, Q., Xu, Z., … Whittaker, A. K. (2014). Self-assembled magnetic luminescent hybrid micelles containing rare earth Eu for dual-modality MR and optical imaging. J. Mater. Chem. B, 2(5), 546-555. doi:10.1039/c3tb21381aGarland, E. R., Rosen, E. P., Clarke, L. I., & Baer, T. (2008). Structure of submonolayer oleic acid coverages on inorganic aerosol particles: evidence of island formation. Physical Chemistry Chemical Physics, 10(21), 3156. doi:10.1039/b718013fSmolensky, E. D., Park, H.-Y. E., Zhou, Y., Rolla, G. A., Marjańska, M., Botta, M., & Pierre, V. C. (2013). Scaling laws at the nanosize: the effect of particle size and shape on the magnetism and relaxivity of iron oxide nanoparticle contrast agents. Journal of Materials Chemistry B, 1(22), 2818. doi:10.1039/c3tb00369hKodama, R. . (1999). Magnetic nanoparticles. Journal of Magnetism and Magnetic Materials, 200(1-3), 359-372. doi:10.1016/s0304-8853(99)00347-9Bean, C. P., & Livingston, J. D. (1959). Superparamagnetism. Journal of Applied Physics, 30(4), S120-S129. doi:10.1063/1.2185850Li, Q., Kartikowati, C. W., Horie, S., Ogi, T., Iwaki, T., & Okuyama, K. (2017). Correlation between particle size/domain structure and magnetic properties of highly crystalline Fe3O4 nanoparticles. Scientific Reports, 7(1). doi:10.1038/s41598-017-09897-5Roca, A. G., Morales, M. P., O’Grady, K., & Serna, C. J. (2006). Structural and magnetic properties of uniform magnetite nanoparticles prepared by high temperature decomposition of organic precursors. Nanotechnology, 17(11), 2783-2788. doi:10.1088/0957-4484/17/11/010Coey, J. M. D. (1971). Noncollinear Spin Arrangement in Ultrafine Ferrimagnetic Crystallites. Physical Review Letters, 27(17), 1140-1142. doi:10.1103/physrevlett.27.1140Linderoth, S., Hendriksen, P. V., Bo/dker, F., Wells, S., Davies, K., Charles, S. W., & Mo/rup, S. (1994). On spin‐canting in maghemite particles. Journal of Applied Physics, 75(10), 6583-6585. doi:10.1063/1.356902Daou, T. J., Grenèche, J. M., Pourroy, G., Buathong, S., Derory, A., Ulhaq-Bouillet, C., … Begin-Colin, S. (2008). Coupling Agent Effect on Magnetic Properties of Functionalized Magnetite-Based Nanoparticles. Chemistry of Materials, 20(18), 5869-5875. doi:10.1021/cm801405nSerna, C. J., Bødker, F., Mørup, S., Morales, M. P., Sandiumenge, F., & Veintemillas-Verdaguer, S. (2001). Spin frustration in maghemite nanoparticles. Solid State Communications, 118(9), 437-440. doi:10.1016/s0038-1098(01)00150-8Laurent, S., Dutz, S., Häfeli, U. O., & Mahmoudi, M. (2011). Magnetic fluid hyperthermia: Focus on superparamagnetic iron oxide nanoparticles. Advances in Colloid and Interface Science, 166(1-2), 8-23. doi:10.1016/j.cis.2011.04.003N. T. Thanh , Magnetic Nanoparticles From Fabrication to Clinical Applications , CRC Press , Boca Raton , 2012Hergt, R., & Dutz, S. (2007). Magnetic particle hyperthermia—biophysical limitations of a visionary tumour therapy. Journal of Magnetism and Magnetic Materials, 311(1), 187-192. doi:10.1016/j.jmmm.2006.10.1156Dong-Hyun Kim, Thai, Y. T., Nikles, D. E., & Brazel, C. S. (2009). Heating of Aqueous Dispersions Containing ${\hbox{MnFe}}_{2}{\hbox{O}}_{4}$ Nanoparticles by Radio-Frequency Magnetic Field Induction. IEEE Transactions on Magnetics, 45(1), 64-70. doi:10.1109/tmag.2008.2005329Rosensweig, R. E. (2002). Heating magnetic fluid with alternating magnetic field. Journal of Magnetism and Magnetic Materials, 252, 370-374. doi:10.1016/s0304-8853(02)00706-0D. Ortega and Q. A.Pankhurst , in Nanoscience: Volume 1: Nanostructures through Chemistry , ed. P. O'Brien , The Royal Society of Chemistry , Cambridge , 2013 , vol. 1 , pp. 60–88Wildeboer, R. R., Southern, P., & Pankhurst, Q. A. (2014). On the reliable measurement of specific absorption rates and intrinsic loss parameters in magnetic hyperthermia materials. Journal of Physics D: Applied Physics, 47(49), 495003. doi:10.1088/0022-3727/47/49/495003Guibert, C., Dupuis, V., Peyre, V., & Fresnais, J. (2015). Hyperthermia of Magnetic Nanoparticles: Experimental Study of the Role of Aggregation. The Journal of Physical Chemistry C, 119(50), 28148-28154. doi:10.1021/acs.jpcc.5b07796Henoumont, C., Laurent, S., & Vander Elst, L. (2009). How to perform accurate and reliable measurements of longitudinal and transverse relaxation times of MRI contrast media in aqueous solutions. Contrast Media & Molecular Imaging, 4(6), 312-321. doi:10.1002/cmmi.294Biju, S., Gallo, J., Bañobre‐López, M., Manshian, B. B., Soenen, S. J., Himmelreich, U., … Parac‐Vogt, T. N. (2018). A Magnetic Chameleon: Biocompatible Lanthanide Fluoride Nanoparticles with Magnetic Field Dependent Tunable Contrast Properties as a Versatile Contrast Agent for Low to Ultrahigh Field MRI and Optical Imaging in Biological Window. Chemistry – A European Journal, 24(29), 7388-7397. doi:10.1002/chem.201800283Rohrer, M., Bauer, H., Mintorovitch, J., Requardt, M., & Weinmann, H.-J. (2005). Comparison of Magnetic Properties of MRI Contrast Media Solutions at Different Magnetic Field Strengths. Investigative Radiology, 40(11), 715-724. doi:10.1097/01.rli.0000184756.66360.d3Guldris, N., Argibay, B., Kolen’ko, Y. V., Carbó-Argibay, E., Sobrino, T., Campos, F., … Rivas, J. (2016). Influence of the separation procedure on the properties of magnetic nanoparticles: Gaining in vitro stability and T1–T2 magnetic resonance imaging performance. Journal of Colloid and Interface Science, 472, 229-236. doi:10.1016/j.jcis.2016.03.040Hu, F., & Zhao, Y. S. (2012). Inorganic nanoparticle-based T1 and T1/T2 magnetic resonance contrast probes. Nanoscale, 4(20), 6235. doi:10.1039/c2nr31865bDaldrup-Link, H. E. (2017). Ten Things You Might Not Know about Iron Oxide Nanoparticles. Radiology, 284(3), 616-629. doi:10.1148/radiol.2017162759Hu, F., Jia, Q., Li, Y., & Gao, M. (2011). Facile synthesis of ultrasmall PEGylated iron oxide nanoparticles for dual-contrastT1- andT2-weighted magnetic resonance imaging. Nanotechnology, 22(24), 245604. doi:10.1088/0957-4484/22/24/245604Tegafaw, T., Xu, W., Ahmad, M. W., Baeck, J. S., Chang, Y., Bae, J. E., … Lee, G. H. (2015). Dual-modeT1andT2magnetic resonance imaging contrast agent based on ultrasmall mixed gadolinium-dysprosium oxide nanoparticles: synthesis, characterization, andin vivoapplication. Nanotechnology, 26(36), 365102. doi:10.1088/0957-4484/26/36/365102Im, G. H., Kim, S. M., Lee, D.-G., Lee, W. J., Lee, J. H., & Lee, I. S. (2013). Fe3O4/MnO hybrid nanocrystals as a dual contrast agent for both T1- and T2-weighted liver MRI. Biomaterials, 34(8), 2069-2076. doi:10.1016/j.biomaterials.2012.11.05

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No abstract availabl

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