Additional file 1: Supplementary Tables. of Whole-body patterns of the range of joint motion in young adults: masculine type and feminine type

Table S1. Age, hand/arm and foot/leg dominances, and experience of sports of the subjects dominances of the subjects. Table S2. Somatometric and sthenometric measurements in the subjects. (DOCX 23 kb

Characteristics of study participants, broken down by populations.

<p>Characteristics of study participants, broken down by populations.</p

The effects of tropical-climate-related SNPs and root-crop-diet-related SNPs on health indicators; general linear model, after adjustment for age, gender, and population differences (also see S2–S6 Tables).

<p>The effects of tropical-climate-related SNPs and root-crop-diet-related SNPs on health indicators; general linear model, after adjustment for age, gender, and population differences (also see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172676#pone.0172676.s002" target="_blank">S2</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172676#pone.0172676.s006" target="_blank">S6</a> Tables).</p

The allele frequencies of five SNPs in populations analyzed in the Human Genome Diversity Project (HGDP) and in this study.

<p>This study analyzed the Kusaghe (rural Melanesian, N = 183), Munda (urban Melanesian, N = 215), and Ravaki peoples (Micronesian, N = 163); Ravaki people were sampled from the Solomon Islands, and their original location is shown on the map. HGDP East Asia (N = 234) includes the following geographic origins: China, Japan, Siberia; HGDP Europe (N = 160): France, Italy, Italy (Bergamo), Orkney Islands, Russia, Russia (Caucasus); HGDP Melanesia (N = 36): Bougainville, Papua New Guinea, HGDP Middle East (N = 146): Israel (Carmel), Israel (Central), Israel (Negev); HGDP North Africa (N = 30): Algeria (Mzab); HGDP Latin America (N = 108): Brazil, Colombia, and Mexico; HGDP South Asia (N = 197): Pakistan; HGDP Southeast Asia (N = 11): Cambodia; and HGDP Sub-Saharan Africa (N = 121): Central African Republic, Democratic Republic of Congo, Kenya, Namibia, Nigeria, Senegal, and South Africa.</p

The effects of tropical-climate-related SNPs and root-crop-diet-related SNPs on the occurrence of diseases; logistic regression analyses, after adjustment for age, gender, and population differences (also see S9–S13 Tables).

<p>The effects of tropical-climate-related SNPs and root-crop-diet-related SNPs on the occurrence of diseases; logistic regression analyses, after adjustment for age, gender, and population differences (also see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172676#pone.0172676.s009" target="_blank">S9</a>–<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0172676#pone.0172676.s013" target="_blank">S13</a> Tables).</p

An ancestral haplotype of the human <i>PERIOD2</i> gene associates with reduced sensitivity to light-induced melatonin suppression

<div><p>Humans show various responses to the environmental stimulus in individual levels as “physiological variations.” However, it has been unclear if these are caused by genetic variations. In this study, we examined the association between the physiological variation of response to light-stimulus and genetic polymorphisms. We collected physiological data from 43 subjects, including light-induced melatonin suppression, and performed haplotype analyses on the clock genes, <i>PER2</i> and <i>PER3</i>, exhibiting geographical differentiation of allele frequencies. Among the haplotypes of <i>PER3</i>, no significant difference in light sensitivity was found. However, three common haplotypes of <i>PER2</i> accounted for more than 96% of the chromosomes in subjects, and 1 of those 3 had a significantly low-sensitive response to light-stimulus (<i>P</i> < 0.05). The homozygote of the low-sensitive <i>PER2</i> haplotype showed significantly lower percentages of melatonin suppression (<i>P</i> < 0.05), and the heterozygotes of the haplotypes varied their ratios, indicating that the physiological variation for light-sensitivity is evidently related to the <i>PER2</i> polymorphism. Compared with global haplotype frequencies, the haplotype with a low-sensitive response was more frequent in Africans than in non-Africans, and came to the root in the phylogenetic tree, suggesting that the low light-sensitive haplotype is the ancestral type, whereas the other haplotypes with high sensitivity to light are the derived types. Hence, we speculate that the high light-sensitive haplotypes have spread throughout the world after the Out-of-Africa migration of modern humans.</p></div

Comparison of the distributions of melatonin suppression for the genotypes of <i>PER2</i>.

<p>The thick middle lines in the boxes represent the medians, the tops and bottoms of the boxes represent the third and the first quartiles, respectively, and the lower and upper error bars indicate the minimum and the maximum values. One dot represents one subject, and the numbers of individuals, N, are shown in the parentheses. In the case of heterozygote, differently colored dots represent different genotypes. The Kruskal-Wallis test and Scheffe’s method of multiple comparison tests were conducted, and the pairs of Hap1-Hap3 and Hap2-Hap3 homozygotes showed significant differences.</p

SNPs examined in the <i>PER2</i> gene and frequencies of the haplotypes in the global populations.

<p>(A) Relative map for the six SNPs examined. The SNPs examined in this study are numbered serially, and their rs numbers, alleles reported, and allele frequencies of JPT in the HapMap database are shown. For the SNP6, the amino acid change (Gly/Glu) has been reported in dbSNP. (B) Ancestral allele frequencies of SNPs in <i>PER2</i>. The allele frequency data from Japan are divided into four groups: subjects with physiological data, Northern Kyushu, Ryukyu, and JPT. Those are compared to three approximate geographic populations: East Asia (CHB, CHS), Europe (CEU, FIN, GBR, IBS, TSI) and Africa (LWK, YRI) from the 1000 Genomes Project database. (C) Haplotype frequencies of <i>PER2</i> in each geographical region. The numbers of local populations included in the geographical region are shown in parentheses. The N represents the numbers of the individuals. The combined frequencies of the remaining haplotype (Residuals) are less than 1.0% in all the geographical regions, indicating that each haplotype in the residual is extremely uncommon among the samples examined.</p

Summary of subjects’ physiological characteristics.

<p>Summary of subjects’ physiological characteristics.</p

Ancestry populations for the Japanese and continental Asians modeled using the ADMIXTURE program.

<p>Eight ancestry populations are inferred. In this bar plot, each individual is represented by a vertical bar, which is colored by eight segments that indicate the proportion contributed by the corresponding ancestry populations. The individuals are sorted by 23 continental Asian clusters (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0185487#pone.0185487.s014" target="_blank">S4 Table</a> for details) and nine Japanese clusters. The bar widths are halved for Japanese individuals, to fit the plot in space. The inset shows color-codes for eight ancestry populations and geographic areas of clusters, to which the individual population mainly contributes.</p