23 research outputs found
Examining c-di-GMP and possible quorum sensing regulation in Pseudomonas fluorescens SBW25:links between intra and inter-cellular regulation benefits community cooperative activities such as biofilm formation
Bacterial success in colonizing complex environments requires individual response to micro-scale conditions as well as community-level cooperation to produce large-scale structures such as biofilms. Connecting individual and community responses could be achieved by linking the intracellular sensory and regulatory systems mediated by bis-(3β²-5β²)-cyclic dimeric guanosine monophosphate (c-di-GMP) and other compounds of individuals with intercellular quorum sensing (QS) regulation controlling populations. There is growing evidence to suggest that biofilm formation by many pseudomonads is regulated by both intra and intercellular systems, though in the case of the model Pseudomonas fluorescens SBW25 Wrinkly Spreader in which mutations increasing c-di-GMP levels result in the production of a robust cellulose-based air-liquid interface biofilm, no evidence for the involvement of QS regulation has been reported. However, our recent review of the P. fluorescens SBW25 genome has identified a potential QS regulatory pathway and other QSβassociated genes linked to c-di-GMP homeostasis, and QS signal molecules have also been identified in culture supernatants. These findings suggest a possible link between c-di-GMP and QS regulation in P. fluorescens SBW25 which might allow a more sophisticated and responsive control of cellulose production and biofilm formation when colonising the soil and plant-associated environments P. fluorescens SBW25 normally inhabits.ΠΠ½Π°Π»ΠΈΠ· Ρ-Π΄ΠΈ-ΠΠΠ€ ΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠ³ΠΎ ΡΡΠ²ΡΡΠ²Π° ΠΊΠ²ΠΎΡΡΠΌΠ° Ρ Pseudomonas fluorescens SBW 25: ΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ Π²Π½ΡΡΡΠΈ ΠΈ ΠΌΠ΅ΠΆΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠ΅ΠΉ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΠΊΠΎΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΌΡ ΠΏΠΎΠ²Π΅Π΄Π΅Π½ΠΈΡ Π² ΡΠΎΠΎΠ±ΡΠ΅ΡΡΠ²Π΅ ΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π±ΠΈΠΎΠΏΠ»ΡΠ½ΠΊΠΈΠ£ΡΠΏΠ΅ΡΠ½ΠΎΡΡΡ Π±Π°ΠΊΡΠ΅ΡΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΡΠ»ΠΎΠΆΠ½ΡΡ
ΡΠΊΠΎΠ½ΠΈΡ ΡΡΠ΅Π±ΡΠ΅Ρ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΡΠ²Π΅ΡΠ° Π½Π° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΡΠ»ΠΎΠ²ΠΈΠΉ Π½Π° ΠΌΠΈΠΊΡΠΎΡΡΠΎΠ²Π½Π΅ ΡΠ°Π²Π½ΠΎ ΠΊΠ°ΠΊ ΠΈ ΠΊΠΎΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ Π½Π° ΡΡΠΎΠ²Π½Π΅ ΡΠΎΠΎΠ±ΡΠ΅ΡΡΠ²Π° Π΄Π»Ρ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ ΡΠ°ΠΊΠΈΡ
ΠΊΡΡΠΏΠ½ΠΎ ΠΌΠ°ΡΡΡΠ°Π±Π½ΡΡ
ΡΡΡΡΠΊΡΡΡ ΠΊΠ°ΠΊ Π±ΠΈΠΎΠΏΠ»ΡΠ½ΠΊΠΈ. ΠΠΎΠΎΡΠ΄ΠΈΠ½Π°ΡΠΈΡ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΡΡ
ΠΎΡΠ²Π΅Ρ ΠΎΠ² ΠΈ ΠΎΡΠ²Π΅ΡΠΎΠ² ΡΠΎΠΎΠ±ΡΠ΅ΡΡΠ²Π° ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ Π΄ΠΎΡΡΠΈΠ³Π½ΡΡΠ° ΠΏΡΡΠ΅ΠΌ ΡΠ²ΡΠ·ΡΠ²Π°Π½ΠΈΡ Π²Π½ΡΡΡΠΈΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
ΡΠ΅Π½ΡΠΎΡΠ½ΡΡ
ΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΡ
ΡΠΈΡΡΠ΅ΠΌ, ΠΎΠΏΠΎΡΡΠ΅Π΄ΡΠ΅ΠΌΡΡ
Π±ΠΈΡ-(3',5')-ΡΠΈΠΊΠ»ΠΈΡΠ΅ΡΠΊΠΈΠΌ Π΄ΠΈΠΌΠ΅ΡΠ½ΡΠΌ Π³ΡΠ°Π½ΠΎΠ·ΠΈΠ½ΠΌΠΎΠ½ΠΎΡΠΎΡΡΠ°ΡΠΎΠΌ (Ρ-Π΄ΠΈ-ΠΠΠ€) ΠΈ Π΄ΡΡΠ³ΠΈΠΌΠΈ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡΠΌΠΈ ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΡΠΌΠΎΠ² Ρ ΠΌΠ΅ΠΆΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠ΅ΠΉ - ΡΡΠ²ΡΡΠ²ΠΎΠΌ ΠΊΠ²ΠΎΡΡΠΌΠ° (Π§Π), ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΡΡΠ΅ΠΌ ΠΏΠΎΠΏΡΠ»ΡΡΠΈ Ρ. ΠΠ°ΠΊΠ°ΠΏΠ»ΠΈΠ²Π°Π΅ΡΡΡ Π²ΡΡ Π±ΠΎΠ»ΡΡΠ΅ Π΄ΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΡΡΠ² ΡΠΎΠ³ΠΎ, ΡΡΠΎ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ Π±ΠΈΠΎΠΏΠ»Π΅Π½ΠΊΠΈ ΠΌΠ½ΠΎΠ³ΠΈΠΌΠΈ ΠΏΡΠ΅Π²Π΄ΠΎΠΌΠΎΠ½Π°Π΄Π°ΠΌΠΈ ΡΠ΅Π³ΡΠ»ΠΈΡΡΠ΅ΡΡΡ ΠΊΠ°ΠΊ Π²Π½ΡΡΡΠΈ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΌΠΈ, ΡΠ°ΠΊ ΠΈ ΠΌΠ΅ΠΆ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΠΌΠΈ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠΌΠΈ ΡΠΈΡΡΠ΅ΠΌΠ°ΠΌΠΈ, Ρ
ΠΎΡΡ Π² ΡΠ»ΡΡΠ°Π΅ ΠΌΠΎΠ΄Π΅Π»ΡΠ½ΠΎΠΉ Pseudomonas fluorescens SBW25 Wrinkly Spreader, Ρ ΠΊΠΎΡΠΎΡΠΎΠΉ ΠΌΡΡΠ°ΡΠΈΠΈ, ΠΏΠΎΠ²ΡΡΠ°ΡΡ ΠΈΠ΅ ΡΡΠΎΠ²Π½ΠΈ Ρ-Π΄ΠΈ-ΠΠΠ€, ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡ ΠΊ ΡΠΎΠ·Π΄Π°Π½ΠΈΡ ΠΏΡΠΎΡΠ½ΠΎΠΉ ΡΠ΅Π»Π»ΡΠ»ΠΎΠ·Π½ΠΎΠΉ Π±ΠΈΠΎΠΏΠ»ΡΠ½ΠΊΠΈ Π½Π° Π³ΡΠ°Π½ΠΈΡΠ΅ ΡΠ°Π·Π΄Π΅Π»Π° ΡΠ°Π· Π²ΠΎΠ·Π΄ΡΡ
-ΠΆΠΈΠ΄ΠΊΠΎΡΡΡ, Π½Π΅ Π±ΡΠ»ΠΎ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ Π½ΠΈ ΠΊΠ° ΠΊΠΎΠ³ΠΎ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²Π° Π²ΠΎΠ²Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΊΠ²ΠΎΡΡΠΌ-Π·Π°Π²ΠΈΡΠΈΠΌΠΎΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΈΠΈ. ΠΠ΄Π½Π°ΠΊΠΎ Π½Π°Ρ Π½Π΅Π΄Π°Π²Π½ΠΈΠΉ ΠΎΠ±Π·ΠΎΡ Π³Π΅Π½ΠΎΠΌΠ° P. fluorescens SBW25 Π²ΡΡΠ²ΠΈΠ» ΠΏΠΎΡΠ΅Π½ΡΠΈΠ°Π»ΡΠ½ΡΠΉ Π§Π-Π·Π°Π²ΠΈΡΠΈΠΌΡΠΉ ΡΠ΅Π³ΡΠ»ΡΡΠΎΡΠ½ΡΠΉ ΠΏΡ ΡΡ ΠΈ Π΄ΡΡΠ³ΠΈΠ΅ Π§Π-Π·Π°Π²ΠΈΡΠΈΠΌΡΠ΅ Π³Π΅Π½Ρ, ΡΠ²ΡΠ·Π°Π½Π½ΡΠ΅ Ρ Π³ΠΎΠΌΠ΅ΠΎΡΡΠ°Π·ΠΎΠΌ Ρ-Π΄ΠΈ-ΠΠΠ€, Π° ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ Π§Π-ΡΠΈΠ³Π½Π°Π»ΠΈΠ½Π³Π° Π±ΡΠ»ΠΈ ΠΈΠ΄Π΅Π½ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Ρ Π² ΠΊΡΠ»ΡΡΡΡΠ΅. ΠΡΠΈ Π΄Π°Π½Π½ΡΠ΅ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΠΉ ΡΠ²ΡΠ·ΠΈ ΠΌΠ΅ΠΆΠ΄Ρ Ρ-Π΄ΠΈ-ΠΠΠ€-ΡΠ΅Π³ΡΠ»ΡΡΠΈΠ΅ΠΉ ΠΈ Π§Π Ρ P. fluorescens SBW25, ΡΡΠΎ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ Π±ΠΎΠ»Π΅Π΅ ΡΠ»ΠΎΠΆΠ½ΡΠΉ ΠΈ Π³ΠΈΠ±ΠΊΠΈΠΉ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ Π½Π°Π΄ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠ΅ΠΉ ΡΠ΅Π»Π»ΡΠ»ΠΎΠ·Ρ ΠΈ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈ Π΅ΠΌ Π±ΠΈΠΎΠΏΠ»Π΅Π½ΠΊΠΈ ΠΏΡΠΈ ΠΊΠΎΠ»ΠΎΠ½ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΠΎΡΠ² ΠΈ ΡΠΊΠΎΠ½ΠΈΡ, aΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Ρ ΡΠ°ΡΡΠ΅Π½ΠΈΡΠΌ ΠΈ, - Π΅ΡΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ ΡΡΠ΅Π΄Π°ΠΌΠΈ ΠΎΠ±ΠΈΡΠ°Π½ΠΈΡ P. fluorescens SBW25
Ornamental grasses breeding activity in the botanical garden of ural federal university
This paper described the importance of ornamental grasses in modern landscape design; the use of new non- traditional plant species and the need for new modern varieties in order to improve plant breeding under challenging climatic conditions in the region. Methods were tested and developed in the botanical garden of the Ural Federal University at different stages of plant breeding. It is an Anatomo-morphological method (test) of rapid selection of initial seed production according to the principle of correlation relationship between floral and vegetative traits. The main and typical features of future cultivar of wide-spread Leymus divaricatus (Drobow) Tzvelev are presented by an identity card of future cultivar. Β© 2021 Author(s).Ministry of Education and Science of the Russian Federation, Minobrnauka, (FEUZ-2020-0057)This work was supported by the Ministry of Science and Higher Education of the Russian Federation as part of the state assignment Κ FEUZ-2020-0057
eDNA inactivation and biofilm inhibition by the polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl)
The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here, we investigated how different biocides affect the release of eDNA from mature biofilms of two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles. The effect of four groups of biocides, alcohols, hydrogen peroxide, quaternary ammonium compounds, and the polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl), was evaluated using PA and SA biofilms. Most biocides, except for PHMG-Cl and 70% ethanol, caused substantial eDNA release, and PHMG-Cl was found to block biofilm development when used at concentrations of 0.5% and 0.1%. This might be associated with the formation of DNAβPHMG-Cl complexes as PHMG-Cl is predicted to bind to AT base pairs by molecular docking assays. PHMG-Cl was found to bind high-molecular DNA and plasmid DNA and continued to inactivate DNA on surfaces even after 4 weeks. PHMG-Cl also effectively inactivated biofilm-associated antibiotic resistance gene eDNA released by a pan-drug-resistant Klebsiella strain, which demonstrates the potential of a polymeric biocide as a new surface-active agent to combat the spread of antibiotic resistance in hospital settings
p73 Regulates Neurodegeneration and Phospho-Tau Accumulation during Aging and Alzheimer's Disease
SummaryThe genetic mechanisms that regulate neurodegeneration are only poorly understood. We show that the loss of one allele of the p53 family member, p73, makes mice susceptible to neurodegeneration as a consequence of aging or Alzheimer's disease (AD). Behavioral analyses demonstrated that old, but not young, p73+/β mice displayed reduced motor and cognitive function, CNS atrophy, and neuronal degeneration. Unexpectedly, brains of aged p73+/β mice demonstrated dramatic accumulations of phospho-tau (P-tau)-positive filaments. Moreover, when crossed to a mouse model of AD expressing a mutant amyloid precursor protein, brains of these mice showed neuronal degeneration and early and robust formation of tangle-like structures containing P-tau. The increase in P-tau was likely mediated by JNK; in p73+/β neurons, the activity of the p73 target JNK was enhanced, and JNK regulated P-tau levels. Thus, p73 is essential for preventing neurodegeneration, and haploinsufficiency for p73 may be a susceptibility factor for AD and other neurodegenerative disorders
ΠΠ»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈΠ½Π΄Π΅ΠΊΡ ΡΠ°ΡΠΈΠΎΠ½ΠΎΠ² ΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΠΈΡΠ°Π½ΠΈΡ ΠΈ ΡΠΈΡΠΊ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° Ρ ΠΌΡΠΆΡΠΊΠΎΠ³ΠΎ Π³ΠΎΡΠΎΠ΄ΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ° (ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅)
Objective: to study the value of the glycemic index (GI) diets in men of Novosibirsk, the link it withΒ nutritional factors and assessment of the risk of metabolic syndrome (MS). A cross-sectional study was carriedΒ out in the framework of the international project HAPIEE, random representative sample of the unorganizedpopulation of Novosibirsk included 3699 men without diabetes.Materials and metods. Assessment of the actual power β frequency method. To calculate the GI diets usedΒ data from the International tables of glycemic index and glycemic load, as well as data published Lobykinoy E.N.Β et al. The criteria for the metabolic syndrome were made in accordance with the Russian national guidelinesΒ All-Russian Scientific Society of Cardiology. For comparison accepted criteria National Cholesterol EducationΒ Program β Adult Treatment Panel III (NCEP-ATP III, 2001). Statistical analysis performed using theΒ application package SPSS.11.5 (Bonferroni test procedure GLM). Estimation of odds ratios was performedΒ using binary logistic regression in quartiles of glycemic index. Differences were considered statisticallyΒ significant at p < 0,05. The average glycemic index diets (M Β± SD) in men Novosibirsk averaged 55,2 Β± 3,3,Β the minimum value of the first quartile β 50,8 Β± 2,4 and a maximum value in the fourth β 58,9 Β± 1,3. AgainstΒ the background of lower-GI group first quartile, the proportion of persons with obesity, hypertriglyceridemia and hyperglycemia was significantly greater than in the quartile with the highest GI. Accordingly, the odds ratio (OR) the risk of metabolic syndrome in the fourth quartile minimum: OR = 0,70 (CI 0,58; 0,84; p < 0,001) (criteria All-Russian Scientific Society of Cardiology) and OR = 0,69 (CI 0,54; 0,88; p < 0,001) (criteria NCEP-ATP III, 2001). Under the first quartile of the GI is set higher consumption of animal products and atherogenic saturated fatty acids.Β Conclusion. Against the background of higher consumption of animal products, fats in general, betterΒ consumption profile group of plant products in the first quartile reduces GI diet, but not the risk of developingΒ MS. There is evidence that high intake of fat, including Meat and meat products leads to the risk of developingΒ MS. Along with recommendations to reduce sugar consumption, increase the number of products with βslowβΒ carbohydrates, should strengthen the nutrition component of the fat correction. Particular attention shouldΒ be paid to reduce salt intake.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ Π³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ½Π΄Π΅ΠΊΡΠ° (ΠΠ) ΡΠ°ΡΠΈΠΎΠ½ΠΎΠ² ΠΏΠΈΡΠ°Π½ΠΈΡ ΠΈ ΡΠΈΡΠΊΠ°Β ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° Ρ ΠΌΡΠΆΡΠΈΠ½ Π³. ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ°.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΠΎΡΡ-ΡΠ΅ΠΊΡΠΈΠΎΠ½Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΎ Π² ΡΠ°ΠΌΠΊΠ°Ρ
ΠΌΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠ³ΠΎΒ ΠΏΡΠΎΠ΅ΠΊΡΠ° HAPIEE. ΠΠ·ΡΡΠ°Π»Π°ΡΡ ΡΠ»ΡΡΠ°ΠΉΠ½Π°Ρ ΡΠ΅ΠΏΡΠ΅Π·Π΅Π½ΡΠ°ΡΠΈΠ²Π½Π°Ρ Π²ΡΠ±ΠΎΡΠΊΠ° Π½Π΅ΠΎΡΠ³Π°Π½ΠΈΠ·ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡΒ Π³. ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ°, 3 699 ΠΌΡΠΆΡΠΈΠ½, ΡΡΠ΅Π΄Π½ΠΈΠΉ Π²ΠΎΠ·ΡΠ°ΡΡ 58,1 Β± 7,0, Π±Π΅Π· ΡΠ°Ρ
Π°ΡΠ½ΠΎΠ³ΠΎ Π΄ΠΈΠ°Π±Π΅ΡΠ°. ΠΡΠ΅Π½ΠΊΠ° ΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎΒ ΠΏΠΈΡΠ°Π½ΠΈΡ β ΡΠ°ΡΡΠΎΡΠ½ΡΠΉ ΠΌΠ΅ΡΠΎΠ΄. ΠΠ»Ρ ΡΠ°ΡΡΠ΅ΡΠ° ΠΠ ΡΠ°ΡΠΈΠΎΠ½ΠΎΠ² ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π΄Π°Π½Π½ΡΠ΅ ΠΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΡΡ
ΡΠ°Π±Π»ΠΈΡΒ Π³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ½Π΄Π΅ΠΊΡΠ° ΠΈ Π³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠΉ Π½Π°Π³ΡΡΠ·ΠΊΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ Π΄Π°Π½Π½ΡΡ
, ΠΎΠΏΡΠ±Π»ΠΈΠΊΠΎΠ²Π°Π½Π½ΡΡ
ΠΠΎΠ±ΡΠΊΠΈΠ½ΠΎΠΉ Π.Π. ΡΒ ΡΠΎΠ°Π²Ρ. ΠΡΠΈΡΠ΅ΡΠΈΠΈ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π»ΠΈΡΡ Π² ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΠΈΠΈ Ρ Π ΠΎΡΡΠΈΠΉΡΠΊΠΈΠΌΠΈ Π½Π°ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌΠΈΒ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄Π°ΡΠΈΡΠΌΠΈ ΠΠΠΠ. ΠΠ»Ρ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ ΠΏΡΠΈΠ½ΠΈΠΌΠ°Π»ΠΈΡΡ ΠΊΡΠΈΡΠ΅ΡΠΈΠΈ ΠΠ‘ NCEP ATP III, 2001. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠ°Ρ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠ° Π΄Π°Π½Π½ΡΡ
ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΏΠ°ΠΊΠ΅ΡΠ° ΠΏΡΠΈΠΊΠ»Π°Π΄Π½ΡΡ
ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌ SPSS.11.5 (ΠΊΡΠΈΡΠ΅ΡΠΈΠΉΒ ΠΠΎΠ½ΡΠ΅ΡΡΠΎΠ½ΠΈ Π² ΠΏΡΠΎΡΠ΅Π΄ΡΡΠ΅ GLM). ΠΡΠ΅Π½ΠΊΡ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΡΠ°Π½ΡΠΎΠ² OR ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ Π±ΠΈΠ½Π°ΡΠ½ΠΎΠΉΒ Π»ΠΎΠ³ΠΈΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π³ΡΠ΅ΡΡΠΈΠΈ Π² ΠΊΠ²Π°ΡΡΠΈΠ»ΡΡ
Π³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΠ½Π΄Π΅ΠΊΡΠ°. Π Π°Π·Π»ΠΈΡΠΈΡ ΡΡΠΈΡΠ°Π»ΠΈΡΡ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈΒ Π·Π½Π°ΡΠΈΠΌΡΠΌΠΈ ΠΏΡΠΈ p < 0,05. Π‘ΡΠ΅Π΄Π½ΠΈΠΉ Π³Π»ΠΈΠΊΠ΅ΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈΠ½Π΄Π΅ΠΊΡ ΡΠ°ΡΠΈΠΎΠ½ΠΎΠ² ΠΏΠΈΡΠ°Π½ΠΈΡ Ρ ΠΌΡΠΆΡΠΈΠ½ Π³. ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ°Β ΡΠΎΡΡΠ°Π²ΠΈΠ» Π² ΡΡΠ΅Π΄Π½Π΅ΠΌ 55,2 Β± 3,3 Ρ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΡΠΌ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ΠΌ Π² ΠΏΠ΅ΡΠ²ΠΎΠΌ ΠΊΠ²Π°ΡΡΠΈΠ»Π΅ 50,8 Β± 2,4 ΠΈ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΌΒ β 58,9 Β± 1,3 Π² ΡΠ΅ΡΠ²Π΅ΡΡΠΎΠΌ. ΠΠ° ΡΠΎΠ½Π΅ Π±ΠΎΠ»Π΅Π΅ Π½ΠΈΠ·ΠΊΠΎΠ³ΠΎ ΠΠ Π² Π³ΡΡΠΏΠΏΠ΅ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ ΠΊΠ²Π°ΡΡΠΈΠ»Ρ Π΄ΠΎΠ»Ρ Π»ΠΈΡ Ρ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ,Β Π³ΠΈΠΏΠ΅ΡΡΡΠΈΠ³Π»ΠΈΡΠ΅ΡΠΈΠ΄Π΅ΠΌΠΈΠ΅ΠΉ ΠΈ Π³ΠΈΠΏΠ΅ΡΠ³Π»ΠΈΠΊΠ΅ΠΌΠΈΠ΅ΠΉ Π² Π½Π΅ΠΉ Π·Π½Π°ΡΠΈΠΌΠΎ Π±ΠΎΠ»ΡΡΠ΅, ΡΠ΅ΠΌ Π² ΠΊΠ²Π°ΡΡΠΈΠ»Π΅ Ρ ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΡΠΌ ΠΠ.Β Π‘ΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ OR ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ° Π² ΡΠ΅ΡΠ²Π΅ΡΡΠΎΠΌ ΠΊΠ²Π°ΡΡΠΈΠ»Π΅ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΡΠ΅: 0,70Β (ΠΠ 0,58; 0,84; Ρ < 0,001) (ΠΊΡΠΈΡΠ΅ΡΠΈΠΈ ΠΠ‘ ΠΠΠΠ, 2009) ΠΈ 0,69 (ΠΠ 0,54; 0,88; Ρ < 0,001) (ΠΊΡΠΈΡΠ΅ΡΠΈΠΈ ΠΠ‘Β NCEP ATP III, 2001). Π Π³ΡΡΠΏΠΏΠ΅ ΠΏΠ΅ΡΠ²ΠΎΠ³ΠΎ ΠΊΠ²Π°ΡΡΠΈΠ»Ρ ΠΠ ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΎΠ΅ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Β ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² ΠΈ Π°ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΡΡ
Π½Π°ΡΡΡΠ΅Π½Π½ΡΡ
ΠΆΠΈΡΠ½ΡΡ
ΠΊΠΈΡΠ»ΠΎΡ.Β ΠΠ° ΡΠΎΠ½Π΅ Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ², ΠΆΠΈΡΠΎΠ² Π² ΡΠ΅Π»ΠΎΠΌ Π±ΠΎΠ»Π΅Π΅ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΉ ΠΏΡΠΎΡΠΈΠ»ΡΒ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡ ΡΠ°ΡΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Π² ΠΏΠ΅ΡΠ²ΠΎΠΌ ΠΊΠ²Π°ΡΡΠΈΠ»Π΅ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΠ ΡΠ°ΡΠΈΠΎΠ½Π°,Β Π½ΠΎ Π½Π΅ ΡΠΈΡΠΊΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠ½Π΄ΡΠΎΠΌΠ°. ΠΠ°ΡΡΠ΄Ρ Ρ ΡΠ΅ΠΊΠΎΠΌΠ΅Π½Π΄Π°ΡΠΈΡΠΌΠΈ ΠΏΠΎ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡΒ ΡΠ°Ρ
Π°ΡΠ°, ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Ρ Β«ΠΌΠ΅Π΄Π»Π΅Π½Π½ΡΠΌΠΈΒ» ΡΠ³Π»Π΅Π²ΠΎΠ΄Π°ΠΌΠΈ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΡΡΠΈΠ»ΠΈΡΡ ΠΊΠΎΡΡΠ΅ΠΊΡΠΈΡΒ ΠΆΠΈΡΠΎΠ²ΠΎΠ³ΠΎ ΠΊΠΎΠΌΠΏΠΎΠ½Π΅Π½ΡΠ° ΠΏΠΈΡΠ°Π½ΠΈΡ. ΠΡΠΎΠ±ΠΎΠ΅ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ»Π΅Π΄ΡΠ΅Ρ ΡΠ΄Π΅Π»ΠΈΡΡ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΡ ΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΡ ΡΠΎΠ»ΠΈ
Retinoic Acid Functions as a Key GABAergic Differentiation Signal in the Basal Ganglia
Retinoic acid (RA) is essential for the generation of GABAergic inhibitory neurons in the mouse forebrain, and RA treatment of embryonic stem cells induces the production of GABAergic neurons
Polymorphism taqib (rs708272) gene protein cholesterol ester transfer (CETP) in women with autoimmune thyroiditis with the result of hypothyroidism
Objective: To study the polymorphism TaqIB (rs708272) gene protein cholesterolΠΎl ester transfer (CETP) in women with autoimmune thyroiditis.
Materials and Methods: We examined 98 women with autoimmune thyroiditis, mean age 55.2 Β± 7.8 years, disease duration 8.0 Β± 6.4 years, menopause duration 6.4 Β± 3.5 years. The average dose of L-thyroxine 84.3 Β± 28.5. Control group consisted of 135 women of similar age without thyroid pathology. Polymorphism of TaqIB (rs708272) CETP gene formed by PCR RFLP on published by technique.
Results: As a result, it was found that in the group of women with AIT Π1Π1 and Π2Π2 genotypes determined in 37.8 % and 21.4 %. Win Π1Π2 heterozygotes in patients with hypothyroidism compared with the control group was significantly lower: 40.8 % and 54.8 %, p = 0.035. Average levels of total cholesterol, LDL cholesterol, HDL cholesterol, triglycerides were not significantly different in carriers of different genotypes of TaqIB polymorphism (rs708272) CETP gene in the two groups
Analysis of some biomarkers of dyslipidemia in obstructive sleep apnea and obese patients
The aim of this study was to examine the relationship between obstructive sleep apnea (OSA) and the biomarkers of dyslipidemia in obese patients. 66 patients diagnosed with OSA and obesity (Body mass index (BMI) β₯ 30 kg/m2) were enrolled. The study demonstrated higher level of total cholesterol and low-density lipoproteins in patients with severe OSA. There was further significant correlation between elevated levels of triglycerides and low-density lipoproteins and symptoms of hyperactivity of the sympathetic nervous system such as reduced heart rate variability and circadian index according to the daily ECG monitoring