1,146 research outputs found

    NDAS Hardware Translation Layer Development

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    The NASA Data Acquisition System (NDAS) project is aimed to replace all DAS software for NASA s Rocket Testing Facilities. There must be a software-hardware translation layer so the software can properly talk to the hardware. Since the hardware from each test stand varies, drivers for each stand have to be made. These drivers will act more like plugins for the software. If the software is being used in E3, then the software should point to the E3 driver package. If the software is being used at B2, then the software should point to the B2 driver package. The driver packages should also be filled with hardware drivers that are universal to the DAS system. For example, since A1, A2, and B2 all use the Preston 8300AU signal conditioners, then the driver for those three stands should be the same and updated collectively

    RDM Solutions for Smaller Institutions

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    Learn how to showcase RDM services at your smaller institution. Join us as we host Ryan Clement the Data Services Librarian at Middlebury College and Wendy Shook the Science Data Librarian, at Middlebury College, as they discuss research data management (RDM) services at a smaller liberal arts college. The webinar will introduce challenges and the innovative solutions as described in the paper ā€œTeam Based Data Management Instruction at Small Liberal Arts Collegesā€ and a case study from what is currently being done at Middlebury College and plans for the future

    Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoS

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    <p>Abstract</p> <p>Background</p> <p><it>Borrelia burgdorferi </it>has limited biosynthetic capabilities and must scavenge N-acetylglucosamine (GlcNAc), an essential component of the microbial cell wall, from the surrounding environment. Spirochetes cultured in the absence of free GlcNAc exhibit biphasic growth; however, addition of chitobiose (a dimer of GlcNAc) substitutes for free GlcNAc resulting in a single exponential phase. We evaluated the effect of RpoS and RpoN, the only alternative sigma factors in <it>B. burgdorferi</it>, on biphasic growth and chitobiose utilization in the absence of free GlcNAc. In addition, we investigated the source of GlcNAc in the second exponential phase.</p> <p>Results</p> <p>By comparing the growth of wild-type cells to insertional mutants for <it>rpoS </it>and <it>rpoN </it>we determined that RpoS, but not RpoN, partially regulates both biphasic growth and chitobiose utilization. The <it>rpoS </it>mutant, cultured in the absence of free GlcNAc, exhibited a significant delay in the ability to initiate a second exponential phase compared to the wild type and <it>rpoS </it>complemented mutant. Expression analysis of <it>chbC</it>, which encodes the membrane-spanning protein of the chitobiose phosphotransferase system, suggests the delay is due to the inability of the <it>rpoS </it>mutant to up regulate <it>chbC</it>. Furthermore, supplementing GlcNAc starved cultures with high concentrations (75 or 150 Ī¼M) of chitobiose resulted in biphasic growth of the <it>rpoS </it>mutant compared to a single exponential phase for the wild type and <it>rpoS </it>complemented mutant. In contrast, growth of the <it>rpoN </it>mutant under all conditions was similar to the wild type. 5' Rapid amplification of cDNA ends (5' RACE) revealed the transcriptional start site for <it>chbC </it>to be 42 bp upstream of the translational start site. Analysis of the <it>chbC </it>promoter region revealed homology to previously described RpoD and RpoS <it>B. burgdorferi </it>promoters. We also determined that yeastolate, a component of the growth medium (BSK-II), is not essential for second exponential phase growth.</p> <p>Conclusion</p> <p>Together these results suggest that RpoD and RpoS, but not RpoN, regulate biphasic growth and chitobiose utilization in <it>B. burgdorferi </it>by regulating the expression of the chitobiose transporter (<it>chbC</it>). The data also demonstrate that the second exponential phase observed in wild-type cells in the absence of free GlcNAc is not due to free chitobiose or GlcNAc oligomers present in the medium.</p

    Leadership learning design principles: Co-creating dialogic and critical pedagogy within cohort and community contexts

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    The purpose of this paper is to share our pedagogical evolution as graduate faculty in relationship with increasingly diverse cohort communities of early childhood professionals learning across the landscape of leadership roles engaged with young children, families, and other adults. The early childhood leadership program described in this paper offers a graduate certificate where annually, a cohort of 18-22 early childhood professionals from across Colorado in the United States learn together for 13 months. As faculty, we share a strong commitment to both learning about our teaching and to inviting student voices, the early childhood professionals, through dialogic processes in participatory study as we co-learn and grow our practices of learning and teaching. This paper introduces and explores four leadership learning design principles: (1) identity and agency, (2) socially constructed pedagogy, (3) contextually relevant learning experience, and (4) appreciative stance. As we go, the story will unfold around how we engaged cohort members of the 2019-2020 program year through initial survey reflections and then deepening our shared understanding of these leadership learning design principles through iterations of dialogue after the program was concluded. We end the paper with reflections on how this process of study has ultimately brought us to an awareness and eagerness to engage in relational forms of inquiry, placing student voices at the center with even more intention and depth

    Perancangan Media Informasi Cara Mengatasi Ular Yang Masuk Kedalam Rumah Di Komplek Bukit Datuk Kota Dumai

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    Reciprocity as deliberative capacity: lessons from a citizens deliberation on carbon pricing mechanisms in Australia

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    Australia has seen a deep division in opinion in search of a carbon pricing mechanism. While concepts of carbon taxation and emission trading have comparable public support, climate scepticism is influencing the debates in political and public spheres in downplaying the need for carbon pricing. Public deliberation is a possible engagement option to address the conflict inherent in climate policy preferences. This research explores the way that a deliberative forum involving twenty-four Australians promoted effective communication between participants through which conflict between policy preferences became more tangible. While the forum did not eliminate disagreement in preferences in the choice of carbon pricing mechanisms, participants reached consensus on fundamental principles such as the need for trusted sources of information, trusted governance procedures, and transparent accountability by appropriate institutions. Shared political expectations encouraged dialogue and cooperation in discussions by enhancing reciprocal understanding. Two sceptical participants who originally had strong opinions different from the rest of the group managed to find common ground. Public deliberative forums that are conducive to reciprocal communication are able to provide a mechanism for joint problem-solving processes that are less adversarial and more responsive to the range of people\u27s preferences. Keywords: public deliberation, consensus, emission trading, carbon tax, deliberative democracy, Australi

    Evaluation of multi-segmental kinematic modelling in the paediatric foot using three concurrent foot models

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    Background: Various foot models are used in the analysis of foot motion during gait and selection of the appropriate model can be difficult. The clinical utility of a model is dependent on the repeatability of the data as well as an understanding of the expected error in the process of data collection. Kinematic assessment of the paediatric foot is challenging and little is reported about multi-segment foot models in this population. The aim of this study was to examine three foot models and establish their concurrent test-retest repeatability in evaluation of paediatric foot motion during gait. Methods: 3DFoot, Kinfoot and the Oxford Foot Model (OFM) were applied concurrently to the right foot and lower limb of 14 children on two testing sessions. Angular data for foot segments were extracted at gait cycle events and peaks and compared between sessions by intraclass correlation coefficient (ICC) with 95% confidence intervals (95% CI) and standard error of measurement (SEM). Results: All foot models demonstrated moderate repeatability: OFM (ICC 0.55, 95% CI 0.16 to 0.77), 3DFoot (ICC 0.47, 95% CI 0.15 to 0.64) and Kinfoot (ICC 0.43, 95% CI āˆ’0.03 to 0.59). On the basis of a cut-off of 5Ā°, acceptable mean error over repeated sessions was observed for OFM (SEM 4.61Ā° Ā± 2.86Ā°) and 3DFoot (SEM 3.88Ā° Ā± 2.18Ā°) but not for Kinfoot (SEM 5.08Ā° Ā± 1.53Ā°). Reliability of segmental kinematics varied, with low repeatability (ICC < 0.4) found for 14.3% of OFM angles, 22.7% of 3DFoot angles and 37.6% of Kinfoot angles. SEM greater than 5Ā° was found in 26.2% of OFM, 15.2% of 3DFoot, and 43.8% of Kinfoot segmental angles. Conclusion: Findings from this work have demonstrated that segmental foot kinematics are repeatable in the paediatric foot but the level of repeatability and error varies across the segments of the different models. Information on repeatability and test-retest errors of three-dimensional foot models can better inform clinical assessment and advance understanding of foot motion during gait
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