Bythotrephes Cederstroemi in Ohio Reservoirs: Evidence from Fish Diets

Author Institution: Aquatic Ecology Laboratory, Department of Evolution, Ecology, and Organismal Biology, The Ohio State UniversityThe invading European cladoceran Bythotrephes cederstroemi, previously reported in North America from the Great Lakes and inland lakes in Ontario and Minnesota, was found in diets of juvenile largemouth bass (Micropterus salmoides) from three Ohio reservoirs in the Ohio River drainage, representing a potential expansion of the range of this exotic species. In summer 1996 samples, we found B. cederstroemi in the stomachs of small largemouth bass (37.0-115.0 mm total length) from Knox, Pleasant Hill, and Tappan Reservoirs, all within the Muskingum River watershed. Although uncommon, B. cederstroemi occurred in diets collected during mid July and late August

From Star Charts to Stoneflies: Detecting Relationships in Continuous Bivariate Data

Within many ecological systems, relationships between controlling factors and associated response variables are complex. In many cases, the response should vary little when the controlling factor exerts strong effects. Conversely, when the effect of the controlling factor is weak or absent, the response may vary greatly with effects of other factors. Correlation or regression analyses often may not be appropriate for testing these relationships, because variance of the response changes with values of the controlling factor. We suggest using a technique from the astronomy literature, a two-dimensional Kolmogorov- Smirnov (2DKS) test, to detect relationships in bivariate data with these patterns of variance. This technique successfully identified simulated bivariate data composed of paired independent values as having nonsignificant relationships and simulated bivariate data in which mean and variance of y was constrained at high levels of x as having significant relationships. Using these simulations and examples from aquatic and terrestrial systems, we demonstrate that the 2DKS is a robust test for detecting nonrandom patterns in bivariate distributions that commonly arise in many ecological systems.Support for this research was provided to E. A. Marschall by National Science Foundation (NSF) grant DEB 9410327, to J. E. Garvey and R. A. Wright by NSF grant DEB 9407859 and Federal Aid in Sport Fish Restoration Project F-69-P (administered jointly by the U.S. Fish and Wildlife Service and the Ohio Division of Wildlife, to R. A. Stein, The Ohio State University), and to J. E. Garvey by a Presidential Fellowship from The Ohio State University

Overwinter Growth and Survival of Largemouth Bass: Interactions among Size, Food, Origin, and Winter Severity

Winter severity (temperature, duration, and photocycle), geographic origin, food availability, and initial body size likely influence growth, survival, and, therefore, recruitment of age-0 largemouth bass Micropterus salmoides. We collected age-0 largemouth bass (70–160 mm total length) from low (33N), intermediate (40N), and high (45N) latitudes throughout their natural range (origin), and we subjected all three groups of fish to three experimental winters that mimicked these latitudes (N = 9 largemouth bass per treatment). Within each winter and origin, one-half of the largemouth bass were fed fish prey, whereas the remaining one-half were starved. Winter strongly influenced survival; overall survival rates in the high-, intermediate-, and low-latitude winters were 34.9, 59.4, and 61.1%, respectively (x2 test, P < 0.05). Largemouth bass from 33N suffered high mortality in the high-latitude winter. Across all winters, more fed fish (64.5%) survived than did starved fish (38.1%) (x2 test, P =100 mm) size classes revealed that more small fish died than did large fish in the low- and high-latitude winters, but this was not the case in the middle-latitude winter. Wet weights (g) of fed largemouth bass increased, remained constant, and declined in the low-, intermediate-, and high-latitude winters, respectively. Wet weights and total energy content (kJ) of fed individuals were consistently higher than those of their starved counterparts in all winters. However, energy density (kJ/g) of fed individuals often declined to levels similar to those of starved largemouth bass. Winter temperature combined with duration likely dictate the northern limit of largemouth bass by reducing growth, even when food is abundant. Because survival of individuals from the low latitude was poor in higher latitude winters, stocking southern largemouth bass in northern systems may translate to high mortality and perhaps to degradation of physiological tolerances of local populations through hybridization.This research was funded by National Science Foundation grant DEB 9407859 and associated Research Experiences for Undergraduates supplement to A. H. Fullerton and by Federal Aid in Sport Fish Restoration project F-69-P, administered jointly by the U.S. Fish and Wildlife Service and the Ohio Division of Wildlife. A Presidential Fellowship from The Ohio State University supported J. E. Garvey

Predicting How Winter Affects Energetics of Age-0 Largemouth Bass: How Do Current Models Fare?

During the first winter of life, loss of energy reserves as a function of low feeding activity and scarce prey may contribute to high mortality of age-0 largemouth bass Micropterus salmoides. To explore how two current bioenergetics models predict winter energy depletion, we quantified growth and consumption by age-0 largemouth bass from Alabama, Ohio, and Wisconsin fed maintenance rations in 55-L aquaria in three simulated winters mimicking temperatures and photoperiods at low temperate latitudes (Alabama; 33N), middle latitudes (Ohio; 40N), and high temperate latitudes (Wisconsin; 46N).We compared observed growth in aquaria with that predicted by putting observed consumption into both models. During winter 1995–1996, we validated one of the models with a separate pool experiment (5,800-L) in which age-0 largemouth bass were fed either at 0.5 X or 1.5 X maintenance ration. In aquaria, energy density of the largemouth bass declined in the high- and middle- but not in the low-latitude winter. Though error was slight in the low- and middle-latitude winters for one of the models, both models underestimated growth in the high-latitude winter. To fit the model to the data, the function that estimates weight-specific resting metabolism had to be reduced by about 16%. In pools, where we predicted consumption from observed growth, the model adequately predicted consumption by largemouth bass fed 1.5 X maintenance, but overestimated consumption by 0.5 X maintenance individuals. Current bioenergetics models perform poorly at the cold temperatures (<6C), photoperiods, and low prey abundances typical of high-latitude lakes, likely because metabolic costs are overestimated.This research was funded by National Science Foundation grant DEB 9407859 to R.A.S. and Federal Aid in Sport Fish Restoration Project F-69-P, administered jointly by the U.S. Fish and Wildlife Service and the Ohio Division of Wildlife. A University PostDoctoral Fellowship and a Presidential Fellowship from The Ohio State University supported R.A.W. and J.E.G., respectively, during part of this work

Evaluating How Local- and Regional-Scale Processes Interact to Regulate Growth of Age-0 Largemouth Bass

Regional- and local-scale processes may interact to influence early growth and survival, thereby governing cohort strength. During summer through fall 1994–1996, we assessed how precipitation (a regional-scale process) and prey availability (a local-scale process) influenced piscivory and growth of age-0 largemouth bass Micropterus salmoides in five Ohio reservoirs (190–1,145 ha). We expected early growth to vary with the abundance and relative sizes of age- 0 gizzard shad Dorosoma cepedianum. We collected age-0 largemouth bass and prey fishes every 3 weeks in each reservoir. In 1994, May precipitation was low (total = 4 cm), resulting in low mean daily reservoir discharge (x¯5 reservoirs = 3.6 m3/s). In four reservoirs, stable water levels may have led to successful largemouth bass reproduction and perhaps an early hatch. As such, age-0 largemouth bass in these systems were abundant, consumed gizzard shad, and reached large sizes by fall (15.3 g). In 1995 and 1996, high precipitation (total > 12 cm) and high reservoir discharge [x¯5 reservoirs = 13.8 m3/s (1995), 28.8 m3/s (1996)] in some reservoirs in May likely reduced largemouth bass abundances. Growth during these years was density dependent across reservoirs. When age-0 largemouth bass abundance was low, nonshad prey fish were consumed, and mean fall sizes were similar to those in 1994 (12.0 g). Conversely, fall weights (4.5–7.4 g) declined in reservoirs with increasing largemouth bass density. Surveying May precipitation in Ohio across 48 years revealed that conditions like those in 1994 occurred less than 15% of the time. Because gizzard shad should rarely be available and other prey fish species probably are limited, density-dependent processes should often regulate early piscivory, growth, and potentially, cohort strength in these systems.This research was funded by National Science Foundation grant DEB 9407859 to RAS and Federal Aid in Sport Fish Restoration project F-69-P, administered jointly by the U.S. Fish and Wildlife Service and the Ohio Division of Wildlife. A Postdoctoral Fellowship and Presidential Fellowship from The Ohio State University supported RAW and JEG, respectively, during part of this work

Individual growth and foraging responses of age-0 largemouth bass to mixed prey assemblages during winter

We conducted an outdoor pool experiment at a mid-temperate latitude (Ohio, 40°N) to determine how commonly occurring prey assemblages affect individual foraging and growth of individually marked, age-0 largemouth bass during winter. The treatments were low prey, bluegill prey only, macroinvertebrates only, and bluegill plus macroinvertebrates. Across all treatments, growth in mass (g) was unrelated to body size. Conversely, small individuals lost more energy (kJ) than large counterparts in all but the macroinvertebrate-only treatment. With low prey, overall growth of largemouth bass was negative, with losses varying among individuals by 30% and 60% for mass and energy content, respectively. Counterparts in bluegill-only pools also consistently lost mass and energy, with less variability (15% mass; 30% energy). In the macroinvertebrate-only treatment, 31% of individuals gained mass, reflecting the greatest range in mass (100%) and energy (60%) change. With macroinvertebrates plus bluegill, overall growth was generally negative, with intermediate variance among individuals. Variation in growth among individuals typically increased with the frequency that prey occurred in diets during sampling. Apparently, some individuals were inactive, foraged infrequently, and consistently lost intermediate quantities of mass and energy. Others were active and foraged with variable success. Because activity and growth vary among individuals as a function of prey composition during winter, prey assemblages during this season will affect patterns of first-year survival and cohort strength.Funding for this project was provided by National Science Foundation grant DEB 9407859 to R.A.S. and Federal Aid in Sport Fish Restoration Project F-69-P, administered jointly by the U.S. Fish and Wildlife Service and Ohio Division of Wildlife